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제주도 주변해역에서 발생하는 해양사고의 유형과 원인에 관한 연구
안영화,최찬문,김동근 제주대학교 해양과환경연구소 2001 해양과환경연구소 연구논문집 Vol.25 No.-
1.380 marine incidents occurred in the adjacent sea of Jeju Island, between 1990 - 1999, these were analysed using data from the National Maritime Police Agency. The occurrence rate of marine incidents is fishing vessels about 93%, which of form is the engine troubles 61.6% of the whole and these are human factors resulting from careless operation and poor engine maintenance, this accounted for the main pattern of incidents and great number of them occurred with fishing vessels per a year average are about 138, that if higher than the another seas. On research about marine security kind of work present condition of fish vessel crew in Juju Island is that each long line and books occupy 42% the whole license of crew is that small vessel driver 60%, six degree 35% and most of marine incidents in the fishing vessel cause frequently the engine troubles, propeller troubles lack of ability of repair and crews almost posses the small license
敎養課程으로서의 「人口와 未來」開設 및 運營에 관한 實驗硏究 : 梨花女子大學校를 中心으로
康宇哲,徐洸善,田贊和,金在恩,蘇興烈 이화여자대학교 한국문화연구원 1979 韓國文化硏究院 論叢 Vol.33 No.-
This is a research report of the research that led to the opening of a population education course at Ewha Womans University. The research itself may be divided into the following four parts: planning for the research ; preparatory work for a population education course; opening of a population course; and evaluation of the population course. In the first stage of the research, the work, as usual, consisted of organizing the research team, setting up the research objectives, planning the research schedule, etc. The project began in the Fall of 1974, and concluded in the Fall of 1978. In the second stage of the research, the work was carried out for the preparation of a course in population education. Several sessions of faculty conference, in which more than 40 professors from various fields of specialization participated, were held in order to determine the specific objectives of the proposed course, to draw up a syllabus, and to prepare for a textbook for the course. When a rough sketch of the syllabus, and the contents of the course was made a student group was organized to examine and evaluate it. In the third stage of the research, a population education course titled "Population and Future" was opened as an elective course in the general education curriculum. This was in the Fall of 1875, and the course remained open every semester until now. An anthology with the same title as the course itself was used for reading, and the teaching was carried out as a team teaching by a team of 6-9 professors. In the fourth stage of the research, an evaluative study was taken up in order to evaluate the course with regard to its structure, textbook, teaching method, and its contents, as well as to assess the effectiveness of the course on terms of the students' change in their cognitive and emotive or attitudinal response to population problem.
Kim, Hyun-Jung,Ji, Bo-Ram,Kim, Ji-Soo,Lee, Han-Na,Ha, Dong-Ho,Kim, Chan-Wha Springer 2012 In vitro cellular & developmental biology Animal Vol.48 No.3
<P>Mesenchymal stem cells (MSCs) hold great promise for use in cell-based therapies because of their multipotency and simple methods for in vitro expansion. However, during in vitro expansion, MSCs will age and lose their multipotency and proliferation capability. Previous studies have reported that calorie restriction (CR) increases proliferation of MSCs and decreases apoptosis. Therefore, in this study, we examined the effect of low glucose (LG) on human bone marrow-derived MSCs. Proliferation under low glucose (LG, 1.4 mM) conditions was compared with that under normal glucose (NG, 5.5 mM) conditions. In addition, comparative studies of population doubling (PD), β-galactosidase (β-GAL) activity, reactive oxygen species (ROS) generation and differentiation capacity (osteocytes and adipocytes) in NG and LG conditions were performed. In addition, protein expression patterns were compared between NG and LG conditions and several proteins were found to be up- or down-regulated under the glucose restriction condition (LG condition). As a result, CR does not seem to have a significant effect on proliferation, ROS generation, glucose consumption concentration, population doublings, and adipogenic differentiation of MSCs. Interestingly, however, the differentiation potential into osteocytes was maintained under CR and a lower senescence-associated β-galactosidase (β-GAL) activity was observed under CR than under the NG condition. In addition, we determined three up-regulated proteins (aldehyde dehydrogenase, neuropolyprptide h3, and prolyl 4-hydroxylase alpha subunit) and seven down-regulated proteins (laminin-binding protein, actin, sec 13 protein, alpha soluble N-ethylmaleimide-sensitive fusion protein (NSF)- attachment protein (SNAP), manganese superoxide dismutase, proteasome alpha 1 subunit, and ribosomal protein S12) via two-dimensional electrophoresis analysis. These results imply that differentially expressed proteins under the LG condition may provide further information on the aging and differentiation of stem cells.</P>
Analysis of Glycated Serum Proteins in Type 2 Diabetes Patients with Nephropathy
Kim, Mi-Ryung,Yu, Shin-Ae,Kim, Mi-Yeon,Choi, Kyung Mook,Kim, Chan-Wha 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
The aim of this study was to screen for proteins that are susceptible to glycation under hyperglycemic conditions in patients with type 2 diabetic nephropathy. Serum proteins were analyzed by a proteomic approach using two-dimensional electrophoresis (2-DE) and ESI-Q-TOF MS/MS. Gels were stained with Pro-Q Emerald 488 to analyze the serum glycoproteome, followed by silver nitrate to examine the total serum proteome. Patient sera were divided into four groups according to their microalbuminuria index: type 2 diabetics with normoalbuminuria, microalbuminuria, and overt nephropathy, and healthy subjects. When the HbA1c levels of the diabetic groups were examined, groups with higher HbA1c exhibited higher fructosamine levels, suggesting that the loss of glycemic control affected the glycation of serum proteins. The proteins that became glycated under poor glycemic control were PEDF, apolipoprotein J precursor, hemopexin, immunoglobulin mu heavy chain, and immunoglobulin kappa chain. As albuminuria increased, a marker of kidney damage, the levels of glycated prekallikrein and complement factor C4B3 also increased. The glycated proteins identified in this study may provide the foundation for the development of novel markers of diabetes, hyperglycemia, and diabetic complications.