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- 저자 : Cao Mei-He (검색결과 5 건)
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Cao Mei-He,Nie Zi-Ling,Ai Sheng,Xu Jie 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3
Phase-shifted full-bridge DC/DC converter is widely used in the medium and high power DC/DC conversion, and its efciency is an important index to evaluate the performance of the converter. Phase-shifted full-bridge circuit can easily realize the zero voltage switching (ZVS) of the power devices, but there are also problems such as duty-cycle loss and lagging bridge arms that make it difcult to achieve a wide range of ZVS. The application of active auxiliary network can efectively solve the above problems, but it introduces additional loss. This paper analyzes the phase-shifted full-bridge DC/DC converter with active auxiliary network, the duty cycle-loss suppression and the additional circuit loss, optimizes the circuit parameters to reduce the loss and improve the overall efciency of the converter, verifes the theoretical analysis through experiments. Finally, the efciency advantages of the study for high current output applications are clarifed.
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Qiang Cao,Shumin He,Yuanyuan Deng,Dapeng Zhu,Xiaodong Cui,Guolei Liu,Huai-Jin Zhang,Shishen Yan,Yanxue Chen,Liangmo Mei 한국물리학회 2014 Current Applied Physics Vol.14 No.5
Raman scattering spectroscopy has been performed on high quality Co-doped ZnO epitaxial films, which were grown on Al2O3 (0001) by oxygen-plasma assisted molecular beam epitaxy. Raman measurements revealed two local vibration modes (LVMs) at 723 and 699 cm1 due to the substitution of Co2þ in wurtzite ZnO lattice. The LVM at 723 cm1 is found to be an elemental sensitive vibration mode for Co substitution. The LVM at 699 cm1 can be attributed to enrichment of Co2þ bound with oxygen vacancy, the cobalteoxygen vacancyecobalt complexes, in Zn1xCoxO films associated with ferromagnetism. The intensity of LVM at 699 cm1, as well as saturated magnetization, enhanced after the vacuum annealing and depressed after oxygen annealing.
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Concentrated Multi-nozzle Electrospinning
Yuekun Zheng,Huatang Cao,Zhou Zhou,Xuecui Mei,Lingke Yu,Xiaojun Chen,Gonghan He,Yang Zhao,Dezhi Wu,Daoheng Sun 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6
The multi-nozzle electrospinning is under extensive investigations because it is an easy way to enhance theproductivity and also feasible to produce special structure fibers such as core-shell fibers and to fabricate composite fibers ofthose polymers that cannot form blend solution in common solvent. Control over the multi-nozzle electrospinning fibersdeposition has attracted increasing attentions. The most common method was to use the auxiliary electrode. However, theconcentrated effect of the works of control multi-nozzle electrospinning deposit was inconspicuous. To enhance thecontrolling of multi-nozzle electrospinning deposition, a set-up based oppositely charged electrospinning was designed. Inthis set-up the air flow was used to transport neutralized nanofibers. This electrospinning method was named oppositelycharged and air auxiliary electrospinning (OCAAES). The capacity of OCAAES in deposition area and pattern controllingwere investigated. By the OCAAES, concentrated and several patterned nanofibers deposition were fabricated. Resultsshowed that nanofiber deposition area and pattern of multi-nozzle electrospinning could be controlled actively, and nanofiberdeposition could be fabricated in a quick thickening rate.
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Production and Optimization of a Kiwi Pectin Methylesterase Inhibitor in Pichia pastoris GS115
Qian Liu,Wentao Xu,Shiwen Han,Dongyan Cao,Xiaoyun He,Kunlun Huang,Xiaohong Mei 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6
Gene sequence coding of a kiwi pectin methylesteraseinhibitor was optimized, synthesized, and expressedin Pichia pastoris GS115 based on P. pastoris preferredcodon usage. The expression level of the recombinantprotein (kwPMEI) increased by 89.74% after codonoptimization. Expression conditions of recombinant strainswere optimized. The highest production of kwPMEI wasachieved using 0.8% sorbitol (added every 24 h), 0.05%oleic acid (added at the beginning of induction), and 0.5%methanol (added every 12 h). kwPMEI was purified usingNi2+ chelating affinity chromatography and 17 mg of theprotein was harvested from 60 mL of a culture supernatant. Activity analysis showed that kwPMEI efficiently inhibitedthe activity of different plant PMEs. High expression levelsand purification of kwPMEI will promote applications infruit and vegetable juices.
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Expression and Prognostic Role of MEKK3 and pERK in Patients with Renal Clear Cell Carcinoma
Chen, Qi,Lu, Hong-sheng,Gan, Mei-fu,Chen, Lan-xi,He, Kai,Fan, Guang-min,Cao, Xue-quan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6
Mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 3 (MEKK3) is an important serine/threonine protein kinase and a member of the MAPK family. MEKK3 can effectively activate the MEK/ERK signaling pathway and promote an autocrine growth loop critical for tumor genesis, cell proliferation, terminal differentiation, apoptosis and survival. To explore the relationship between MEKK3 and cell apoptosis, clinicopathology and prognosis, we characterize the expression of MEKK3, pERK and FoxP3 in the renal clear cell carcinoma (RCCC). Protein expression was detected by tissue microarray and immunochemistry in 46 cases of RCCC and 28 control cases. Expression levels of CD3+,CD3+CD4+,CD3+CD8+,CD4+CD25+, CD4+CD25+ FoxP3+ were assessed by flow cytometry and analyzed for their association with pathological factors, correlation and prognosis in RCCC. Expression of MEKK3, pERK and FoxP3 was significantly up-regulated in RCCC as compared to control levels (p<0.01), associated with pathological grade (p<0.05)and clinical stage (p<0.05). CD4+CD25+ Foxp3+ Treg cells were also significantly increased in RCCC patients (p<0.05). Cox multivariate regression analysis showed that MEKK3, pERK expression and patholigical stage were independent prognostic factors in patients with RCCC (p<0.05). MEKK3 can be used as an important marker of early diagnosis and prognostic evaluation in RCCC. It may be associated with imbalance of anti-tumor immunity and overexpression of pERK. Expression of MEKK3 and pERK are significantly increased in RCCC, with protein expression and clinical stage acting as independent prognostic factors.
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