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야생참깨에서 agrobacterium rhizogenes 유도 모상근의 배양조건
Byung Ohg Ahn(安炳玉),Tetsuya Asamizu(淺水哲也),Kyo ji Yamada(山田恭司) 한국육종학회 1997 한국육종학회지 Vol.29 No.2
In order to investigate optimized culture conditions of hairy roots of Sesamum schinzianum (wild sesame) induced by infection of Agrobacterium rhizogenes, ATCC 15834 and MAFF03-01724 were cultured in various media, sucrose concentrations and pH levels. As the results, best growth condition of hairy roots induced by Agrobacterium rhizogenes strains was obtained in 1/2 MS medium free of hormone, 3% sucrose and pH 8.0. Hairy roots induced by Agrobacterium rhizogenes MAFF03-01724 showed better growth of extensive lateral root branches than that of ATCC 15834. In opine analysis, hairy roots induced by Agrobacterium rhizogenes ATCC 15834 was detected as agropine type while mikimopine in that of MAFF03-01724.
Isolation and Characterization of a Ds-tagged liguleless Mutant in Rice (Oryza sativa. L)
Ahn, Byung-Ohg,Ji, Sang-Hye,Yun, Doh-Won,Ji, Hyeon-So,Park, Yong-Hwan,Park, Sung-Han,Lee, Gi-Hwan,Suh, Seok-Cheol,Lee, Myung-Chul The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.4
A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.
Isolation and Characterization of a Ds-tagged liguleless Mutant in Rice (Oryza sativa. L)
Byung-Ohg Ahn,지상혜,Doh-Won Yun,Hyeon-So Ji,Yong-Hwan Park,Sung-Han Park,Gi-Hwan Lee,Seok-Cheol Suh,Myung-Chul Lee 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.4
A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.
손수인(Soo-In Sohn),안병옥(Byung-Ohg Ahn),지희연(Hee-Youn Chi),조병관(Hee-Youn Chi),조민석(Min-Seok Cho),신공식(Kong Sik Shin) 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.5
경제적 및 농업적 장점은 유전자 변형 작물 재배면적의 증가를 가져왔다. 그러나 유전자 변형 작물의 상업적 재배전에 유전자 변형 작물의 인간건강 및 환경에 미칠 잠재적 위해성에 대한 면밀한 검토가 필수적이다. 본 연구에서는 Bt벼의 토양미생물 군집에 미치는 영향을 조사하였다. 토양화학성분을 분석한 결과, Bt벼와 낙동벼 근권토양 간 화학 성분의 유의성 있는 차이는 없는 것으로 조사되었다. 재배전, 재배초기, 최고 분얼기의 토양미생물 군집밀도를 조사했을 때 Bt벼 근권토양의 세균, 방선균, 진균 군집밀도는 낙동 벼와 유사한 수준으로 나타났다. 시기별 DGGE 분석결과 Bt 벼 근권토양 전체미생물상은 낙동벼와 차이가 없는 것으로 조사되었다. Pyrosequencing을 통한 Bt벼와 낙동벼의 미생 물 군집조성을 조사한 결과 주요 미생물상 분포에 있어서도 매우 유사한 양상을 나타내었다. 위의 결과들을 종합해볼 때 Bt 재배에 따른 토양미생물상에 미치는 영향은 미미한 것으로 사료된다. 수확 후 벼 잔존물이 토양환경에 미치는 영향에 대해서는 좀 더 연구가 진행되어야 할 것이다. The cultivation of genetically modified (GM) crops has increased due to their economic and agronomic advantages. Before commercialization of GM crops, however, we must assess the potential risks of GM crops on human health and environment. The aim of this study was to investigate the possible impact of Bt rice on the soil microbial community. Microbial communities were isolated from the rhizosphere soil cultivated with Bt rice and Nakdong, parental cultivar and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional rice were not significantly different. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures during cultural periods were very similar each other. Analysis of dominant isolates in the rhizosphere cultivated with Bt and Nakdong rice showed that the dominant isolates from the soil of Bt rice and Nakdong belonged to the Proteobacteria, Cloroflexi, Actinobacteria, Firmicutes, and Acidobacteria. These results indicate that the Bt rice has no significant impact on the soil microbial communities during cultivation period. Further study remains to be investigated whether the residue of Bt rice effect on the soil environment.
( Soo In Sohn ),( Young Ju Oh ),( Byung Ohg Ahn ),( Tae Hoon Ryu ),( Hyun Suk Cho ),( Jong Sug Park ),( Ki Jong Lee ),( Sung Dug Oh ),( Jang Yong Lee ) 한국환경농학회 2012 한국환경농학회지 Vol.31 No.1
BACKGROUND: Cultivation of genetically modified(GM) crops rapidly has increased in the global agricultural area. Among those, herbicide resistant GM crops are reported to have occupied 89.3 million hectares in 2010. However, cultivation of GM crops in the field evoked the concern of the possibility of gene transfer from transgenic plant into soil microorganisms. In our present study, we have assessed the effects of herbicide-resistant GM Chinese cabbage on the surrounding soil microbial community. METHODS AND RESULTS: The effects of a herbicide- resistant genetically modified (GM) Chinese cabbage on the soil microbial community in its field of growth were assessed using a conventional culture technique and also culture-independent molecular methods. Three replicate field plots were planted with a single GM and four non-GM Chinese cabbages (these included a non-GM counterpart). The soils around these plants were compared using colony counting, denaturing gradient gel electrophoresis and a species diversity index assessment during the growing periods. The bacterial, fungal and actinomycetes population densities of the GM Chinese cabbage soils were found to be within the range of those of the non-GM Chinese cabbage soils. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The similarities of the bacterial species diversity indices were consistent with this finding. CONCLUSION: These results indicate that soil microbial communities are unaffected by the cultivation of herbicide-resistant GM Chinese cabbage within the experimental time frame.
Protox 제초제저항성 벼 재배가 토양미생물 군집에 미치는 영향
오성덕 ( Sung Dug Oh ),안병옥 ( Byung Ohg Ahn ),김민경 ( Min Kyeong Kim ),손수인 ( Soo In Sohn ),류태훈 ( Tae Hun Ryu ),조현석 ( Hyun Suk Cho ),김창기 ( Chang Gi Kim ),백경환 ( Kyoung Whan Back ),이기종 ( Ki Jong Lee ) 한국환경농학회 2013 한국환경농학회지 Vol.32 No.2
BACKGROUND: Rice (Oryza sativa) is the most important staple food of over half the world`s population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn`t show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protoxgene in soil DNA. CONCLUSION(S): These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.
Sohn, Soo-In,Oh, Young-Ju,Ahn, Byung-Ohg,Ryu, Tae-Hoon,Cho, Hyun-Suk,Park, Jong-Sug,Lee, Ki-Jong,Oh, Sung-Dug,Lee, Jang-Yong The Korean Society of Environmental Agriculture 2012 한국환경농학회지 Vol.31 No.1
BACKGROUND: Cultivation of genetically modified(GM) crops rapidly has increased in the global agricultural area. Among those, herbicide resistant GM crops are reported to have occupied 89.3 million hectares in 2010. However, cultivation of GM crops in the field evoked the concern of the possibility of gene transfer from transgenic plant into soil microorganisms. In our present study, we have assessed the effects of herbicide-resistant GM Chinese cabbage on the surrounding soil microbial community. METHODS AND RESULTS: The effects of a herbicide-resistant genetically modified (GM) Chinese cabbage on the soil microbial community in its field of growth were assessed using a conventional culture technique and also culture-independent molecular methods. Three replicate field plots were planted with a single GM and four non-GM Chinese cabbages (these included a non-GM counterpart). The soils around these plants were compared using colony counting, denaturing gradient gel electrophoresis and a species diversity index assessment during the growing periods. The bacterial, fungal and actinomycetes population densities of the GM Chinese cabbage soils were found to be within the range of those of the non-GM Chinese cabbage soils. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The similarities of the bacterial species diversity indices were consistent with this finding. CONCLUSION: These results indicate that soil microbial communities are unaffected by the cultivation of herbicide-resistant GM Chinese cabbage within the experimental time frame.
KRDD: Korean Rice Ds-tagging Lines Database for Rice(Oryza sativa L. Dongjin)
Chang Kug Kim,Myung Chul Lee,Byung Ohg Ahn,Doh Won Yun,Ung Han Yoon,Seok Cheol Suh,은무영,Jang Ho Hahn 한국유전체학회 2008 Genomics & informatics Vol.6 No.2
The Korean Rice Ds-tagging lines Database (KRDD) is designed to provide information about Ac/Ds insertion lines and activation tagging lines using japonica rice. This database has provided information on 18,158 Ds lines, which includes the ID, description, photo image, sequence information, and gene characteristics. The KRDD is visualized using a web-based graphical view, and anonymous users can query and browse the data using the search function. It has four major menus of web pages: (i) a Blast Search menu of a mutant line; Blast from rice Ds-tagging mutant lines; (ii) a primer design tool to identify genotypes of Ds insertion lines; (iii) a Phenotype menu for Ds lines, searching by identification name and phenotype characteristics; and (iv) a Management menu for Ds lines.