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야생참깨에서 agrobacterium rhizogenes 유도 모상근의 배양조건
Byung Ohg Ahn(安炳玉),Tetsuya Asamizu(淺水哲也),Kyo ji Yamada(山田恭司) 한국육종학회 1997 한국육종학회지 Vol.29 No.2
In order to investigate optimized culture conditions of hairy roots of Sesamum schinzianum (wild sesame) induced by infection of Agrobacterium rhizogenes, ATCC 15834 and MAFF03-01724 were cultured in various media, sucrose concentrations and pH levels. As the results, best growth condition of hairy roots induced by Agrobacterium rhizogenes strains was obtained in 1/2 MS medium free of hormone, 3% sucrose and pH 8.0. Hairy roots induced by Agrobacterium rhizogenes MAFF03-01724 showed better growth of extensive lateral root branches than that of ATCC 15834. In opine analysis, hairy roots induced by Agrobacterium rhizogenes ATCC 15834 was detected as agropine type while mikimopine in that of MAFF03-01724.