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- 저자 : Borui Wang (검색결과 5 건)
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Yongzhen Wang,Borui Wang,Dandan Xu,Meng Zhang,Xiaohua Zhang,Deguo Wang 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.4
Background: Due to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever. Objectives: The objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV. Methods: LMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples. Results: Our results showed that the LMTIA assay could detect the 104 dilution of DNA extracted from the positive reference serum sample, which was the same as that of the commercial real-time PCR kit. The coincidence rate between the two assays was 100%. Conclusions: The LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.
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Borui Zhou,Zihan Wang,Xueju Shen 한국광학회 2020 Current Optics and Photonics Vol.4 No.1
In this paper, a new method is presented to measure the refractive index of single plain glass or multilayered materials, based on a laser frequency-shifted confocal feedback microscope. Combining the laser frequency-shifted feedback technique and the confocal effect, the method can attain high axialpositioning accuracy, stability and sensitivity. Measurements of different samples are given, including N-BK7 glass, Silica plain glass, and a microfluidic chip with four layers. The results for N-BK7 glass and Silica plain glass show that the measurement uncertainty in the refractive index is better than 0.001. Meanwhile, the feasibility of this method for multilayered materials is tested. Compared to conventional methods, this system is more compact and has less difficulty in sample processing, and thus is promising for applications in the area of refractive-index measurement.
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Zhou, Borui,Wang, Zihan,Shen, Xueju Optical Society of Korea 2020 Current Optics and Photonics Vol.4 No.1
In this paper, a new method is presented to measure the refractive index of single plain glass or multilayered materials, based on a laser frequency-shifted confocal feedback microscope. Combining the laser frequency-shifted feedback technique and the confocal effect, the method can attain high axial-positioning accuracy, stability and sensitivity. Measurements of different samples are given, including N-BK7 glass, Silica plain glass, and a microfluidic chip with four layers. The results for N-BK7 glass and Silica plain glass show that the measurement uncertainty in the refractive index is better than 0.001. Meanwhile, the feasibility of this method for multilayered materials is tested. Compared to conventional methods, this system is more compact and has less difficulty in sample processing, and thus is promising for applications in the area of refractive-index measurement.
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Minghao Yu,Wei Wang,Jiafeng Yao,Borui Zheng 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.10
The objective of the present study is the development of a comprehensive air chemical kinetic model that includes 11 species and 54 chemical reactions for the numerical investigation of air nonequilibrium inductively coupled plasmas. The two-dimensional, compressible Navier-Stokes equations coupled with the electromagnetic-field equations were employed to describe the fun- damental characteristics of an inductive plasma. Dunn-Kangs 32 chemical-reaction model of air was reconstructed and used as a comparative model. The effects of the different chemical kinetic models on the ow field were analyzed and discussed at identical/different working pressures. The results theoretically indicate that no matter the working pressure is low or high, the use of the 54 chemical kinetic model presented in this study is a better choice for the numerical simulation of a nonequilibrium air ICP.
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The Shearing Behavior of Artificial Soil Extracted from Loess
( Chongyang Gao ),( Ling Xu ),( Dongdong Yan ),( Yili Wang ),( Borui Lv ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
Loess, as a typical silty soil, covered an area of 440,000km2 in China and a lot of work has been done on the study of mechanics and microstructure of the Loess. For better understanding the behavior of Loess, artificial soils we're created by extracting from the Xi’an and Lanzhou loess using water sedimentary method and which were then divided into five groups according to the variation of gradation. A series of triaxial tests were conducted to identify the effect of particle gradation on the behavior of soil within the framework of critical state soil mechanics. The results show that the location of critical state line is first decrease and then increased with the content of the fine particles increasing. And the slope of the critical state lines also shows consistent trend. There exists a phase transformation gradation soil, called C soil, which is the closest to the natural soil on the gradation curve. For A and B soils with more clay content, there are relatively difficult to reach critical state even if their strain reaches 35%, compared to the other types of soil. As the content of coarse particles increases, the soil generally exhibits more pronounced dilatancy, and the properties are closer to fine sand. This paper is of great significance to provide a reference for better understanding of the behavior of loess in the geotechnique engineering application.
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