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- 저자 : Bonakdar Shahin (검색결과 4 건)
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Moraveji, Marzie,Keshvari, Hamid,Karkhaneh, Akbar,Bonakdar, Shahin,Hadi, Amin,Haghighipour, Nooshin Techno-Press 2021 Advances in nano research Vol.10 No.6
This study aimed to develop a collagen/polycaprolactone (CP) fibrous scaffold decorated with Graphene (Gr) nanoplatelets (Gr-CP). In previous studies, accessibility of cells to the surface of Gr nanoplatelet was missed. Nanofibers were prepared by electrospinning which sprayed Gr nanoplatelets (1 wt.%) to synthesize the Gr-CP scaffold. Fourier transform infrared spectroscopy (FTIR) was utilized for investigation of chemical structure. Tensile tests were performed to study the influence of Gr on the mechanical properties of scaffolds. Cell differentiation was analyzed based on MAP2 and TUJ1 expression levels using real-time PCR technique in 6 groups. The variables examined in this experiment was the neural differentiating chemical medium, low-frequency electromagnetic field (LFEMF; 50Hz, 1mT) and Gr. Based on the results, Young's modulus, tensile strength and work of fracture ratio of the Gr-CP were 1.68, 2.41 and 1.42 times higher than those of the CP scaffold, respectively. MTT assay outcomes were indicative of scaffold cytocompatibility. The group treated with all three factors exhibited the highest MAP2 expression level compared to other groups. Based on the obtained results, exposing stem cells to the combined treatment of Gr and LFEMF can be used as a promising method to induce neuronal differentiation.
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Mahsa Janmohammadi,Mohammad Sadegh Nourbakhsh,Shahin Bonakdar 한국섬유공학회 2021 Fibers and polymers Vol.22 No.1
Skin tissue engineering is an evolving method to reconstruct skin damages caused by disease, burn or trauma. Inskin tissue engineering, scaffolds should prepare three-dimensional structure for skin cells. Electrospinning technique hasbeen widely applied for producing nano/micro-scale fiber scaffolds in tissue engineering. In this study, electrospun scaffoldsbased on polycaprolactone (PCL) and hyaluronic acid (HA) containing L-ascorbic acid (AA) were fabricated. Morphology,contact angle, functional groups, biodegradability and drug release of the scaffolds were evaluated. L929 fibroblast cellsseeded on nanofibrous scaffolds and cell attachment and viability were evaluated as well. According to the results, the fibersdiameter were less than 180 nm and by adding hyaluronic acid, the hydrophilicity of scaffolds increased and degradation ratewas adjusted. The encapsulation efficiency and successful release of ascorbic acid in nanofibrous scaffolds weredemonstrated. According to the results, the cell growth, proliferation and adhesion of L929 fibroblast cells on the PCL/HA/AA scaffolds were enhanced in comparison with the PCL scaffold. Moreover, PCL/HA (80:20) containing 40 mg of AAnanofibrous scaffold could be potentially applied for skin tissue engineering.
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Rad Elham Shahriari,Eidi Akram,Minai-Tehrani Dariush,Bonakdar Shahin,Shoeibi Shahram 대한약침학회 2022 Journal of pharmacopuncture Vol.25 No.3
Objectives: Oxidative stress plays a key role in chronic and acute brain disorders and neuronal damage associated with Alzheimer disease (AD) and other neurodegeneration symptoms. The neuroprotective effects of berberine and Berberis vulgaris (barberry) root extract against apoptosis induced by hydrogen peroxide (H 2 O 2 ) in the human SH-SY5Y cell line were studied. Methods: The methanolic extraction of barberry root was performed using a maceration procedure. Oxidative stress was induced in SH-SY5Y cells by H 2 O 2 , and an MTT assay was applied to evaluate the neuroprotective effects of berberine and barberry root extract. The cells were pretreated with the half maximal inhibitory concentration (IC 50 ) of each compound (including berberine, barberry root extract, and H 2 O 2 ), and the anti-apoptotic effects of all components were investigated using RT-PCR. Results: The SH-SY5Y cell viability increased in both groups exposed to 75 and 150 ppm barberry extract compared with that in the H 2 O 2 -treated group. The data showed that exposing SH-SY5Y cells to 30 ppm berberine significantly increased the cell viability compared with the H 2 O 2 -treated group; treatment with 150 and 300 ppm berberine and H 2 O 2 significantly decreased the SH-SY5Y cell viability and was associated with berberine cytotoxicity. The mRNA levels of Bax decreased significantly under treatment with berberine at 30 ppm compared with the control group. A significant increase in Bcl-2 expression was observed only after treatment with the IC 50 of berberine. The expression level of Bcl-2 in cells exposed to both berberine and barberry extracts was also significantly higher than that in cells exposed to H 2 O 2 . Conclusion: The outcomes of this study suggest that treatment of SH-SY5Y cells with barberry extract and berberine could suppress apoptosis by regulating the actions of Bcl-2 family members.
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Saeid Reza Shahmoradi,Maryam Kabir Salmani,Hamid Reza Soleimanpour,Amir Hossein Tavakoli,Kazem Hosaini,Nooshin Haghighipour,Shahin Bonakdar 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.1
BACKGROUND: Articular cartilage damage is still a troublesome problem. Hence, several researches have been performed for cartilage repair. The aim of this study was to evaluate the chondrogenicity of demineralized bone matrix (DBM) scaffolds under cyclic hydrostatic pressure (CHP) in vitro. METHODS: In this study, CHP was applied to human bone marrow mesenchymal stem cells (hBMSCs) seeded on DBM scaffolds at a pressure of 5 MPa with a frequency of 0.5 Hz and 4 h per day for 1 week. Changes in chondrogenic and osteogenic gene expressions were analyzed by quantifying mRNA signal level of Sox9, collagen type I, collagen type II, aggrecan (ACAN), Osteocalcin, and Runx2. Histological analysis was carried out by hematoxylin and eosin, and Alcian blue staining. Moreover, DMMB and immunofluorescence staining were used for glycosaminoglycan (GAG) and collagen type II detection, respectively. RESULTS: Real-time PCR demonstrated that applying CHP to hBMSCs in DBM scaffolds increased mRNA levels by 1.3-fold, 1.2-fold, and 1.7-fold (p\0.005) for Sox9, Col2, and ACAN, respectively by day 21, whereas it decreased mRNA levels by 0.7-fold and 0.8-fold (p\0.05) for Runx2 and osteocalcin, respectively. Additionally, in the presence of TGF-b1 growth factor (10 ng/ml), CHP further increased mRNA levels for the mentioned genes (Sox9, Col2, and ACAN) by 1.4-fold, 1.3-fold and 2.5-fold (p\0.005), respectively. Furthermore, in histological assessment, it was observed that the extracellular matrix contained GAG and type II collagen in scaffolds under CHP and CHP with TGF-b1, respectively. CONCLUSION: The osteo-inductive DBM scaffolds showed chondrogenic characteristics under hydrostatic pressure. Our study can be a fundamental study for the use of DBM in articular cartilage defects in vivo and lead to production of novel scaffolds with two different characteristics to regenerate both bone and cartilage simultaneously.
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