Ring-Opening Polymerization of ε-Caprolactone and Cyclohexene Oxide Initiated by Aluminum β-Ketoamino Complexes

Bin Yuan Liu,Hai Qing Li,Chang Sik Ha,Il Kim,Wei Dong Yan 한국고분자학회 2008 Macromolecular Research Vol.16 No.5

Effects of Ribosomal Protein L39-L on the Drug Resistance Mechanisms of Lung Cancer A549 Cells

Liu, Hong-Sheng,Tan, Wen-Bin,Yang, Ning,Yang, Yuan-Yuan,Cheng, Peng,Liu, Li-Juan,Wang, Wei-Jie,Zhu, Chang-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Cancer is a major threat to the public health whether in developed or in developing countries. As the most common primary malignant tumor, the morbidity and mortality rate of lung cancer continues to rise in recent ten years worldwide. Chemotherapy is one of the main methods in the treatment of lung cancer, but this is hampered by chemotherapy drug resistance, especially MDR. As a component of the 60S large ribosomal subunit, ribosomal protein L39-L gene was reported to be expressed specifically in the human testis and human cancer samples of various tissue origins. Materials and Methods: Total RNA of cultured drug-resistant and susceptible A549 cells was isolated, and real time quantitative RT-PCR were used to indicate the transcribe difference between amycin resistant and susceptible strain of A549 cells. Viability assay were used to show the amycin resistance difference in RPL39-L transfected A549 cell line than control vector and null-transfected A549 cell line. Results: The ribosomal protein L39-L transcription level was 8.2 times higher in drug-resistant human lung cancer A549 cell line than in susceptible A549 cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells showed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions and Implications for Practice: The ribosomal protein L39-L gene may have effects on the drug resistance mechanism of lung cancer A549 cells.

Expression and Prognostic Implications of FOXO3a and Ki67 in Lung Adenocarcinomas

Liu, Hong-Bin,Gao, Xiang-Xiang,Zhang, Qing,Liu, Jian,Cui, Yuan,Zhu, Yan,Liu, Yi-Fei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

To investigate the significance of FOXO3a and Ki67 in human lung adenocarcinomas. Envision immunohistochemical staining and Western blotting were used to examine the protein expression of FOXO3a in 127 cases of human lung adenocarcinoma specimens. The positive rate in lung adenocarcinoma (55.9%) was lower than that in normal tissues (80%). We found that the expression of FOXO3a was closely related with the degree of differentiation, TNM staging, lymph node metastasis and survival. In addition, significant differences in the different pathological types of lung adenocarcinoma cases (P<0.01). The FOXO3a positive rate of the acini as the main type (APA) (86.7%) and the lepidic as the main type (LPA) (82.4%) was higher than the solid as the main type (SPA) (50.0%), the papilla as the main type (PPA) (42.9%) and the micropapilla as the main type (MPA) (9.4%). Moreover, the expression of FOXO3a was negatively related with Ki67 expression. Our results suggested that the expression of FOXO3a is closely correlated with the aggressiveness of lung adenocarcinoma. It was indicated that disregulation of FOXO3a might play key roles in the occurrence and development of lung a denocarcinoma and joint detection of the two markers might play an important role in diagnosing tumors.

An Age- and Condition-Dependent Variable Weight Model for Performance Evaluation of Bridge Systems

Yuan Ren,Xiang Xu,Bin Liu,Qiao Huang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

To address the balance problem between indexes within the performance evaluation of bridge systems, this paper develops an age- and condition-based variable weight model (ACVWM). First, the limitations of existing models used for the multi-layer weighted sum method, i.e., constant weight model (CWM) and condition-based variable weight model (CVWM), are presented through case studies, indicating that the weight variation is insufficient to characterize the deterioration law of components. Then, the definition of age-based variable weight is established following the existing concept of condition-based variable weight, which makes weights vary with service ages. Considering the characteristics of bridge assessment, an age-based variable weight model is built up to depict the time-variant trends of index weights with the service age. The variation law of age-based variable weight is discussed by using indexes in the superstructure of suspension bridges. As a result, the weights of replaceable and permanent components behave differently within the bridge service life. Finally, the ACVWM is built up and its effectiveness is verified through the same case studies applied to the CWM and CVWM. Compared with the evaluation results from the CWM and CVWM, the evaluation result of the ACVWM is more in line with the real maintenance strategy. Considering the CVWM in which low initial weights may lead to unsatisfactory weight assignment, the advantage of the proposed ACVWM lies in its capability to adjust initial weights over the service age.

Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China

Bin Yan,Xiafei Liu,Junyuan Wu,Shanshan Zhao,Wumei Yuan,Baoju Wang,Hazi Wureli,Changchun Tu,Chuangfu Chen,Yuanzhi wang 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.4

Bacterial Fruit Rot of Apricot Caused by Burkholderia cepacia in China

Yuan Fang,Bin Li,Fang Wang,Baoping Liu,Zhiyi Wu,Ting Su,Wen Qiu,Guanlin Xie 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4

An unreported disease of apricot was observed in orchards in Zhejiang province, China. Symptoms started as water soaked lesions on the fruit surface. Later, water-soaked areas developed and spread to the entire fruit, resulting in soft rot of the whole fruit. The causal organism isolated from symptomatic fruits was identified as Burkholderia cepacia based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto fruits of apricot. In addition, European plum, Japanese plum, nectarine and kiwifruit were susceptible to the B. cepacia pathogen. However, the B. cepacia pathogen failed to cause any visible symptoms when it was inoculated onto 16 other fruits. This is the first report of a bacterial disease of apricot caused by B. cepacia in China.

Efficacy and Safety of Neurokinin-1 Receptor Antagonists for Prevention of Chemotherapy-Induced Nausea and Vomiting: Systematic Review and Meta-analysis of Randomized Controlled Trials

Yuan, Dong-Mei,Li, Qian,Zhang, Qin,Xiao, Xin-Wu,Yao, Yan-Wen,Zhang, Yan,Lv, Yan-Ling,Liu, Hong-Bin,Lv, Tang-Feng,Song, Yong Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Objectives: Can addition of neurokinin-1 receptor antagonists (NK1-RAs) be considered as an ideal strategy for the prevention of chemotherapy-induced nausea and vomiting (CINV)? Researchers differ on this question. Materials and Methods: Electronic databases were searched for randomized control trials (RCTs) that evaluated the effectiveness and safety of NK1-RAs in preventing CINV. The primary end point was complete response (CR) in the acute, delayed, and overall phases after chemotherapy. Subgroup analyses evaluated the types of NK1-RAs, routines of administration, types of malignancies, regimens used in combination with NK1-RAs, and age of patients included in the studies. The incidences of different types of adverse events were also extracted to estimate the safety of NK1-RAs. Results: A total of 38 RCTs involving 13,923 patients were identified. The CR rate of patients receiving NK-RAs was significantly higher than patients in the control groups during overall phase (70.8% vs 56.0%, P<0.001), acute phase (85.1% vs 79.6%, P<0.001), and delayed phase (71.4% vs 58.2%, P<0.001). There were three studies including patients of children or adolescents, the CR rate was also significantly higher in the treatment group (overall phase: OR=2.807, P<0.001; acute phase: OR=2.863, P =0.012; delayed phase: OR=2.417, P<0.001). For all the other outcomes, patients in the NK1-RAs groups showed improvements compared to the control groups (incidence of nausea: 45.2% vs 45.9%, P<0.001; occurrence of vomiting: 22.6% vs 38.9%, P<0.001; usage of rescue drugs: 23.5% vs 34.1%, P<0.001). The pooled side effects from NK1-RAs did not significantly differ from previous reports and the toxicity rates in patients less than eighteen years old also did not diff between the two groups (P=0.497). However, we found that constipation and insomnia were more common in the patients of control groups, whereas diarrhea and hiccups were more frequently detected in patients receiving NK1-RAs. Conclusions: NK1-RAs improved the CR rate of CINV. They are effective for both adults and children. The use of NK1-RAs might be associated with the appearance of diarrhea and hiccups, while decreasing the possibility of constipation and insomnia.

Exosomes from Murine-derived GL26 Cells Promote Glioblastoma Tumor Growth by Reducing Number and Function of CD8+T Cells

Liu, Zhi-Ming,Wang, Yu-Bin,Yuan, Xian-Hou Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Aim: Brain tumors almost universally have fatal outcomes; new therapeutics are desperately needed and will only come from improved understandins of glioma biology. Methods: Exosomes are endosomally derived 30~100 nm membranous vesicles released from many cell types. Examples from GL26 cells were here purified using density gradient ultracentrifugation and monitored for effects on GL26 tumor growth in C57BL/6j mice (H-2b). Lactate dehydrogenase release assays were used to detect the cytotoxic activity of CD8+T and NK cells. Percentages of immune cells producing intracellular cytokines were analyzed by FACS. Results: In this study, exosomes from murine-derived GL26 cells significantly promoted in vivo tumor growth in GL26-bearing B6 mice. Then we further analyzed the effects of the GL26 cells-derived exosomes on immune cells including CD8+T, CD4+T and NK cells. Inhibition of CD8+T cell cytotoxic activity was demonstrated by CD8+T cell depletion assays in vivo and LDH release assays in vitro. The treatment of mice with exosomes also led to a reduction in the percentages of CD8+T cells in splenocytes as determined by FACS analysis. Key features of CD8+T cell activity were inhibited, including release of IFN-gamma and granzyme B. There were no effects of exosomes on CD4+T cells and NK cells. Conclusion: Based on our data, for the first time we demonstrated that exosomes from murine derived GL26 cells promote the tumor growth by inhibition of CD8+T cells in vivo and thus may be a potential therapeutic target.

A Voronoi cell finite element method for estimating effective mechanical properties of composite solid propellants

Liu-Lei Shen,Zhi-Bin Shen,Hai-Yang Li,Ze-Yuan Zhang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

The mechanical properties of Composite solid propellant (CSP) are the critical material parameters to analyze the structural integrity of propellant grains, and have a significant influence on the life and reliability of solid rocket motors. A Voronoi cell finite element method using an adaptive algorithm in the time domain is proposed for investigating the linear viscoelasticity of CSP in the present paper. A process is brought forward to produce a Representative volume element (RVE) model, which reflects the microstructural features of CSP. Numerical viscoelastic examples are used for varying the accuracy of this method. In addition, finite element simulations are conducted to understand the effect of microstructural morphology and material properties of inclusion on the effective mechanical properties of CSP using the new method. When this method is applied to the design process of CSP, it can reduce the design cost and shorten the design cycle effectively. The current microscopic numerical analysis method can be used to provide guidance for designing and analyzing the mechanical properties of composite viscoelastic materials.

Impedance Modeling and Resonance Suppression Method for Current Source VSG Under Weak Grid Conditions

Bin Zhu-Zuo,Min Sun-Shu,Yuan Liu-Yi,Ping Huang-Shao 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.4

A large proportion of renewable energy is integrated into power system, resulting in the gradual electrifi cation of the power system and appearing the characteristics of a weak power grid. Under weak grid, current source virtual synchronous generator (VSG) is prone to resonance. To ensure stable and uninterrupted operation of renewable energy through power electronic devices connected to grid, this paper proposes a composite control method based on grid voltage feedforward (GVF) and q-axis impedance reshaping (QAIR) for current source VSG converter resonance suppression. Firstly, sequence impedance modeling (SIM) and stability analysis are conducted on current source VSG converter. Under weak grid, the negative resistance eff ect caused by phase-locked loop (PLL) in the low frequency range makes system unstable in grid connection. To eliminate the negative resistance instability caused by PLL, a current source VSG GVF control resonance suppression method is established. SIM of this method and stability analysis are conducted. Secondly, a QAIR resonance suppression method for current source VSG converter is established. SIM of the method is conducted and stability analyzed. On the basis of above two methods, a composite resonance suppression method based on GVF and QAIR is established, and SIM and stability analysis are conducted. Simulation and experimental tests are conducted on the stability of GVF, QAIR, and GVF + QAIR composite resonance suppression current type VSG systems. The test results showed that the method based on GVF + QAIR resonance suppression is more eff ective, compared to GVF/QAIR resonance suppression method. Under weak grid, it has better ability to eliminate negative resistance eff ects and harmonic suppression caused by PLL, further improving grid-connected stability.