Identification of stathmin 1 expression induced by Epstein–Barr virus in human B lymphocytes

Baik, S. Y.,Yun, H. S.,Lee, H. J.,Lee, M. H.,Jung, S. E.,Kim, J. W.,Jeon, J. P.,Shin, Y. K.,Rhee, H. S.,Kimm, K. C.,Han, B. G. Blackwell Publishing Ltd 2007 Cell proliferation Vol.40 No.2

<P>Abstract. </P><P><I>Introduction</I>: The Epstein–Barr virus transforms resting B cells into proliferating lymphoblastoid cells, the origin of cell lines. <I>Method and results</I>: Our cDNA microarray analyses led to the identification of 232 up-regulated and 112 down-regulated genes with more than a 3-fold difference in lymphoblastoid cell lines compared to resting B cells. The functional classification of these genes exhibited the distinct expression signature for cell proliferation, cell cycle and an immune response. Among them, we verified the differential expression of several oncogenes such as <I>stathmin 1</I> (<I>STMN1</I>), <I>RAB27A</I>, <I>RAB9A</I>, <I>BACH1</I> and <I>BACH2</I> using quantitative real-time reverse transcriptase-polymerase chain reactions or Western blot analysis. Expression of <I>STMN1</I> (which is involved in regulation of the microtubule filament system, cell growth and S-phase of cell cycle) was increased in lymphoblastoid cell line as well as in 7-day post-Epstein–Barr virus infection B cells, compared to resting B cells. <I>Conclusion</I>: Thus, this study suggests that Epstein–Barr virus infection induces <I>STMN1</I> expression, which play a role in cell cycle progression and proliferation in the human B lymphocyte.</P>

Shewanella marina sp. nov., isolated from seawater

Park, S. C.,Baik, K. S.,Kim, M. S.,Kim, D.,Seong, C. N. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.8

<P>A motile, rod-shaped, pale-brown-pigmented bacterium, designated strain C4T, was isolated from seawater collected from the South Sea (Republic of Korea). Cells were Gram-negative, facultatively anaerobic, and catalase- and oxidase-positive. The major fatty acids were summed feature 3 (C16:1omega7c and/or iso-C15:0 2-OH; 19.4%), C16:0 (16.3%), C17:1omega8c (9.5%) and iso-C15:0 (7.7%). The DNA G+C content was 40.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain C4T formed a lineage within the genus Shewanella (92.7-96.1% sequence similarity to representative strains of the genus Shewanella) and was part of a distinct branch with the clade comprising Shewanella haliotis DW01T and Shewanella algae ATCC 51192T. Phenotypic characteristics enabled strain C4T to be distinguished from S. haliotis and S. algae. On the basis of the data presented in this study, strain C4T represents a novel species, for which the name Shewanella marina sp. nov. is proposed. The type strain is C4T (=KCTC 22185T=JCM 15074T).</P>

유효 구동력 개념을 이용한 고상 비정질화 반응의 예측에 관한 연구

곽준섭(J. S. Kwak),지응준(E. J. Chi),최정동(J. D. Choi),박상욱(S. W. Park),소명기(M. K. So),이성만(S. M. Lee),백홍구(H. K. Baik) 한국진공학회(ASCT) 1993 Applied Science and Convergence Technology Vol.2 No.1

이원계 박막확산쌍에서 열처리 방법에 의한 고상 비정질화 반응의 경향성을 예측하기 위하여 유효 구동력 개념을 제시하였다. 고상 비정질화 반응은 두 원소의 물리적 혼합물과 비정질상간의 최대 자유에너지차로 주어지는 열역학적 구동력(ΔG_(max))과 확산원소의 원자반경에 대한 기지의 유효 침입형자리 반경의 비로 주어지는 구조적 요소(R_(m/d))가 충족될 때 일어나는 빠른 확산에 의하여 발생된다고 고찰하고, 유효 구동력 기준을 이용하여 금속/금속계 뿐만아니라 금속/실리콘 계의 고상반응에 의한 비정질상 생성 경향성을 예측하고 실험결과들과 비교하여 잘 적용됨을 보였다. 또한, 유효 구동력 기준이 금속/실리콘 계에서 비정질상의 임계 성장두께 경향성의 예측에도 잘 적용됨을 보였다. It is proposed that formation and growth of amorphous interlayer through solid state amorphizing reaction can be predicted by the concept of effective driving force. The effective driving force consists of two factors; i) the thermodynamic driving force given by maximum free energy difference between physical mixture of binary elements and amorphous interlayer (ΔG_(max)) and ii) the structural factor given by a ratio between effective radius of interstitial site in host matrix and atomic radius of diffusing species (R_(m/d)). It is shown that the the criterion of effective driving force is successfully applied in the prediction of the formation of amorphous interlayer in metal/silicon systems as well as that of metal/metal systems from the comparison of experimental data. In addition, the concept of effective driving force can be well applied to predict the growth tendency of amorphous interlayer in metal/silicon systems.

Macrophage M1/M2 regulation by plasma activated NO-WATER

C B LEE,M H KANG,S S JEON,H S UHM,K U BAIK 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Effect of Genetic and Non-Genetic Factors Other Then Disease on Kid Survivability in Goat

Miah, S. G.,Husain, M. S.,Hoque, D. A.,Baik, D. H. 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.3

이 연구는 벵갈 종과 그 교잡종 산양의 번식에 있어서 생시부터 90일령 까지의 생존율에 영향을 미치는 유전적 및 비 유전적인 요인을 규명하기 위하여 수행되었다. 분석에 이용된 모델은 지역, 교배조합, 자양의 성, 출생시 산자형태(1두, 2두, 3두 및 4두), 출생계절(여름 3월∼6월; 우기 7월∼10월; 및 겨울 11월∼2월), 출생연도와 2요인 상호작용의 효과를 포함하였다. 교잡종에 비하여 순종의 생존율은 31일령과 90일령 사이에 현저히 낮은 것으로 유의성(p<0.05)을 나타냈다. 생존율에 대한 지역의 효과는 16∼60일령 기간을 제외하고는 통계적으로 유의적인 차이를 보이지 않았다. 생존율에 대한 성의 효과는 초기 0∼7일령의 기간을 제외하고는 모두 통계적 유의성이 인정되었다. 생존율은 여름에 출생한 것이 가장 높았고 겨울과 우기(rainy season)의 순서로 나타났다. 16일령부터 60일령 사이의 자양의 생존율은 어미의 비유량에 의하여 영향을 받는 것으로 (p<0.05) 나타났고, 400∼600g/day 의 비유량인 경우에 생존율이 가장 높았던 반면 비유량이 80∼200g/day인 어미의 자양이 가장 낮은 생존율을 보였다. 생시체중은 90일령 까지의 모든 성장단계에서 자양의 생존율에 영향을 미치는 주요 요인이었다. 생존율은 모양의 비유량과 그리고 자양의 생시체중과 정의 상관관계를 보였다. 성과 생산지역간 (p<0.01) 또는 성과 출생시 산자형태간(p<0.05)의 상호작용은 통계적 유의성이 있었던 반면에 성과 유전적 그룹간의 상호작용은 유의성이 없었다. This experiment was conducted to investigate the genetic and non-genetic factors affecting kid survivability in goats from birth to 90 days of age. The purebreds had lower survivability than the crossbreds, with significant (p<0.05) difference amongst themselvess for the age of 31 to 90 days. The locational effect on survivability was insignificant for all the periods except 16 to 60 days (p<0.05) of age. The sex and birth type also had significant (p<0.05) effect on survivability for all the periods except 0 to 7 days of age. The survivability was found to be significantly (p<0.05) higher for kids born in summer season followed by those born in winter and rainy season. Milk produced by the does were significantly (p<0.05) affected on the survivability of kids during the period from 16 to 60 days of age. The survivability of kids were highest and lowest having milk yield of doe found to be 400∼600 g/day and 80∼200 g/day, respectively. Birth weight had significant (p<0.05) effect on survivability for all the stages of growth up to 90 days of age. Survivability was positively correlated with does' milk yield as well as kids birth weight. Interactions of sex with location or birth type were significant (p<0.01 and p<0.05, respectively) though interaction between sex and genetic group was insignificant.

Prevalence of sarcopenia and sarcopenic obesity in Korean adults: the Korean sarcopenic obesity study

Kim, T N,Yang, S J,Yoo, H J,Lim, K I,Kang, H J,Song, W,Seo, J A,Kim, S G,Kim, N H,Baik, S H,Choi, D S,Choi, K M Macmillan Publishers Limited 2009 International Journal of Obesity Vol.33 No.8

Objectives:To examine the prevalence of sarcopenia and sarcopenic obesity (SO) as defined by different indices, including appendicular skeletal muscle mass (ASM)/height<SUP>2</SUP>, skeletal muscle mass index (SMI) and residuals for Korean adults, and to explore the association between SO and metabolic syndrome.Methods:Our study sample included 526 participants (328 women, 198 men) for whom complete data on body composition were collected using available dual X-ray absorptiometry. Modified National Cholesterol Education Program Adult Treatment Panel III criteria were used to identify the individuals with metabolic syndrome.Results:The prevalence of sarcopenia and SO is higher in older adults. Using two s.d. of ASM/height<SUP>2</SUP> below reference values from young, healthy adults as a definition of sarcopenia, the prevalence of sarcopenia and SO was 6.3% and 1.3% in older (60 years) men and 4.1% and 0.8% in older women, respectively. The prevalence of sarcopenia using the residuals method was 15.4% in older men and 22.3% in older women. In addition, using two s.d. of SMI, the prevalence of sarcopenia and SO was 5.1% and 5.1%, respectively, in older men and 14.2% and 12.5%, respectively, in older women. Among women, SO subjects defined by the SMI had three times the risk of metabolic syndrome (odds ratios (OR)=3.24, 95% confidence interval (CI)=1.21–8.66) and non-sarcopenic obese subjects had approximately twice the risk of metabolic syndrome (OR=2.17, 95% CI=1.22–3.88) compared with normal subjects. Similar trends were observed in men.Conclusion:The prevalence and cutoff values of sarcopenia and SO in the Korean population were evaluated using different methods. Among the different indices of sarcopenia and SO, SO only defined using the SMI was associated with the risk of metabolic syndrome. As the Korean population gets older and more obese, the problematics of SO need to be elucidate.International Journal of Obesity (2009) 33, 885–892; doi:10.1038/ijo.2009.130; published online 30 June 2009

Anisotropic Conductivity of n-type a-plane GaN films grown on r-plane Sapphire Substrates

J.B. Kim,K.H. Baik,J.S. Son,S.-K. Hong,T.G. Kim,S.M. Hwang 대한전자공학회 2010 ICEIC:International Conference on Electronics, Inf Vol.1 No.1

We investigated the electrical properties of Ti/Al/Ni/Au Ohmic contacts and the anisotropic conductivity of ntype a-plane (11-20) GaN grown on r-plane (1-102) sapphire substrates. The Ohmic contacts and sheet resistances were measured on several wafers using transfer length method (TLM) as a function of azimuthal angles. The best specific contact resistance of 4.34×10?? Ohmㆍ㎠ was achieved after annealing at 900℃. It was found that there was significant electrical anisotropy in a-plane GaN films on r-plane sapphire substrates, and the sheet resistance varied with azimuthal angles. The sheet resistance values in the direction parallel to the m-axis [1-100] are 25%~75% lower than those parallel to the c-axis [0001] directions. Thus, basal stacking faults (BSFs) are offered as a feasible source of the anisotropic mobility in faulted heteroepitaxial a-plane GaN films because of the band-edge discontinuities at the interface between BSFs and wurtzite GaN matrix.

TRIENNIAL GROWTH AND DEVELOPMENT SYMPOSIUM: Molecular mechanisms related to bovine intramuscular fat deposition in the longissimus muscle

Baik, M.,Kang, H. J.,Park, S. J.,Na, S. W.,Piao, M.,Kim, S. Y.,Fassah, D. M.,Moon, Y. S. American Society of Animal Science 2017 Journal of Animal Science Vol. No.

<P>The intramuscular fat (IMF) content of the LM, also known as marbling, is particularly important in determining the price of beef in Korea, Japan, and the United States. Deposition of IMF is influenced by both genetic (e.g., breed, gender, and genotype) and nongenetic factors (e.g., castration, nutrition, stressors, animal weight, and age). Castration of bulls markedly increases deposition of IMF, resulting in improved beef quality. Here, we present a comparative gene expression approach between bulls and steers. Transcriptomic and proteomic studies have demonstrated that the combined effects of increases in lipogenesis, fatty acid uptake, and fatty acid esterification and decreased lipolysis are associated with increased IMF deposition in the LM. Several peripheral tissues (LM, adipose tissues, and the liver) are involved in lipid metabolism. Therefore, understanding the significance of the tissue network in lipid metabolism is important. Here, we demonstrate that lipid metabolism in LM tissues is crucial for IMF deposition, whereas lipid metabolism in the liver plays only a minor role. Metabolism of body fat and IMF deposition in bovine species has similarities with these processes in metabolic diseases, such as obesity in humans and rodents. Extensive studies on metabolic diseases using epigenome modification (DNA methylation, histone modification, and microRNA), microbial metagenomics, and metabolomics have been performed in humans and rodents, and new findings have been reported using these technologies. The importance of applying 'omics' fields (epigenomics, metagenomics, and metabolomics) to the study of IMF deposition in cattle is described. New information on the molecular mechanisms of IMF deposition may be used to design nutritional or genetic methods to manipulate IMF deposition and to modify fatty acid composition in beef cattle. Applying nutrigenomics could maximize the expression of genetic potential of economically important traits (e.g., marbling) in animals.</P>

Influence of electrode modification by Ar⁺ ion beam upon passivation and electrical characteristics in organic light-emitting diodes

Jeong, S. M.,Koo, W. H.,Choi, S. H.,Jo, S. J.,Baik, H. K.,Lee, S.-J.,Song, K. M. MRS MATERIALS RESEARCH SOCIETY 2005 Journal of materials research Vol.20 No.1

Aequorivita capsosiphonis sp. nov., isolated from the green alga Capsosiphon fulvescens, and emended description of the genus Aequorivita

Park, S. C.,Baik, K. S.,Kim, M. S.,Kim, S. S.,Kim, S. R.,Oh, M.-J.,Kim, D.,Bang, B.-H.,Seong, C. N. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.4

<P>A marine bacterial strain, designated A71(T), was isolated from marine algae collected from the South Sea, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A71(T) belonged to the family Flavobacteriaceae and was closely related to Aequorivita antarctica SW49(T) (96.5 % sequence similarity). Cells of strain A71(T) were Gram-negative, aerobic, oxidase-negative, catalase-positive, yellow/orange-pigmented and non-motile. The major fatty acids were iso-C(15 : 0) (20.6 %), iso-C(17 : 1)omega9c (13.3 %), anteiso-C(15 : 0) (13.1 %), iso-C(17 : 0) 3-OH (12.7 %) and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c; 6.6 %). The DNA G+C content was 36.9 mol%. Several phenotypic characteristics served to differentiate the isolate from recognized members of the genus Aequorivita. Data from this polyphasic study clearly demonstrated that strain A71(T) represents a novel species of the genus Aequorivita. The name Aequorivita capsosiphonis sp. nov. is proposed, with strain A71(T) (=KCTC 22183(T) =JCM 15070(T)) as the type strain. In addition, an emended description of the genus Aequorivita is presented.</P>