The Effects of Mitochondrial Transplantation on Sepsis Depend on the Type of Cell from Which They Are Isolated

김규석,( Jinkun Bae ),( Tae Nyoung Chung ),( Yun-seok Kim ),( Han A Reum Lee ),( Ye Jin Park ),( Min Ji Lee ),( Sehwan Mun ),( Chang June Yune ),( Kyuseok Kim ) 대한응급의학회 2023 대한응급의학회 학술대회초록집 Vol.2023 No.2

Association of MicroRNA Biogenesis Genes Polymorphisms with Ischemic Stroke Susceptibility and Post-Stroke Mortality

Jung Oh Kim,Jinkun Bae,Jinkwon Kim,오승헌,Hui Jeong An,In Bo Han,Doyeun Oh,Ok Joon Kim,김남근 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.1

Background and Purpose MicroRNA (miRNA) expression has been examined in multiple conditions, including various cancers, neurological diseases, and cerebrovascular diseases, particularly stroke. Existing evidence indicates that miRNA biosynthesis and function play crucial roles in ischemic stroke physiology and pathology. In this study, we selected six known polymorphisms in miRNAbiogenesis genes; DICER rs13078A>T, rs3742330A>G; DROSHA rs10719T>C, rs6877842G>C; Ran GTPase (RAN) rs14035C>T; exportin 5 (XPO5) rs11077A>C. Methods We analyzed the associations between these polymorphisms and disease status and clinical factors in 585 ischemic stroke patients and 403 controls. Genotyping was performed with the polymerase chain reaction-restriction fragment length polymorphism method. Results The DICER rs3742330A>G (AA vs. AG+GG: adjusted odds ratio [AOR], 1.360; 95% confidence interval [CI], 1.024 to 1.807; P=0.034) and DROSHA rs10719T>C polymorphisms (TT vs.CC: AOR, 2.038; 95% CI, 1.113 to 3.730; P=0.021) were associated with ischemic stroke prevalence. During a mean follow-up of 4.80±2.11 years, 99 (5.91%) of the stroke patients died. In multivariate Cox proportional hazard regression models, a significant association was found between RAN rs14035 and survival of large artery disease patients with ischemic stroke (CC vs. TT: adjusted hazard ratio, 5.978; P=0.015). Conclusions An association was identified between the DICER and DROSHA polymorphisms and ischemic stroke. Specifically, polymorphisms (rs3742330 and rs10719) were more common in stroke patients, suggesting that they may be associated with an increased risk of ischemic stroke.

Effect of Pretreatment with the NADPH Oxidase Inhibitor Apocynin on the Therapeutic Efficacy of Human Placenta-Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage

Min, Saehong,Kim, Ok Joon,Bae, Jinkun,Chung, Tae Nyoung MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.11

<P>Several studies have demonstrated the beneficial effect of mesenchymal stem cells (MSCs) on intracerebral hemorrhage (ICH). Enhancement of the therapeutic efficacy of MSCs in ICH is necessary, considering the diseases high association with mortality and morbidity. Various preconditioning methods to enhance the beneficial properties of MSCs have been introduced. We suggested apocynin, a well-known nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, as a novel preconditioning regimen to enhance the therapeutic efficacy of MSCs in ICH. Rat ICH models were made using bacterial collagenase. 24 h after ICH induction, the rats were randomly divided into apocynin-preconditioned MSC-treated (Apo-MSC), naïve MSC-treated and control groups. Hematoma volume, brain edema, and degenerating neuron count were compared at 48 h after the ICH induction. The expression of tight junction proteins (occludin, zona occludens [ZO]-1) were also compared. Hematoma size, hemispheric enlargement and degenerating neuron count were significantly lower in the Apo-MSC group than in the naïve MSC group (<I>p</I> = 0.004, 0.013 and 0.043, respectively), while the expression of occludin was higher (<I>p</I> = 0.024). Apocynin treatment enhances the therapeutic efficacy of MSCs in ICH in the acute stage, through the improvement of the beneficial properties of MSCs, such as neuroprotection and the reinforcement of endovascular integrity of cerebral vasculature.</P>

The DEXA-SEPSIS study protocol: a phase II randomized double-blinded controlled trial of the effect of early dexamethasone in high-risk sepsis patients

Choi Kihwan,Park Jong Eun,Kim Anhye,Hwang Sojung,Bae Jinkun,Shin Tae Gun,Kim Kyuseok 대한응급의학회 2022 Clinical and Experimental Emergency Medicine Vol.9 No.3

Objective Steroids are used in cases of sepsis, especially in patients experiencing septic shock. However, clinical trials to date have reported contradictory results. Different patient endotypes and variations in the type and dose of steroid may be at fault for this discrepancy, and further investigation is warranted. In this paper, we propose a new DEXA-SEPSIS study design. Methods We plan to conduct a multicenter, double-blinded randomized pilot study (DEXA-SEPSIS) investigating the feasibility and safety of early use of dexamethasone in sepsis. Participants will be high-risk septic patients presenting to the emergency department with a systolic blood pressure of <90 mmHg or serum lactate level of >2 mmol/L. Participants will be randomized to the following three groups: control, 0.1 mg/kg of dexamethasone, or 0.2 mg/kg of dexamethasone per day for 1 to 2 days. The primary outcome will be 28-day mortality. Secondary outcomes will include time to septic shock, shock reversal, additional steroid administration, number of ventilator-free days, use of continuous renal-replacement therapy, length of stay in the intensive care unit and/or hospital, delta Sequential Organ Failure Assessment score on days 3 and 7, superinfection, gastrointestinal bleeding, hypernatremia, and hyperglycemia. Discussion The DEXA-SEPSIS study will provide insight regarding the feasibility and safety of early use of dexamethasone in high-risk sepsis. The results could provide data to design a future phase III study.

중심정맥도관의 우심방 내 위치 분류 및 예측

이창재 ( Chang Jae Lee ),이태림 ( Tae Rim Lee ),정태녕 ( Tae Nyoung Chung ),배진건 ( Jinkun Bae ) 한국보건정보통계학회 2015 보건정보통계학회지 Vol.40 No.3

Objectives: Central venous catheter (CVC) insertion is one of the most frequently performed procedures during critical care. Confirming proper placement of catheter tip is very important during the procedure because right atrial placement of CVC may increase the risk of fatal complications as perforation and cardiac tamponade. This study was performed to check the usefulness of tip-to-carina (TC) distance and to compare the accuracy among various classification models for predicting RA positioning of a CVC. Methods: This study reviewed retrospectively medical records and picture archiving communication system of patients who were given chest computed tomography within 12 hours after CVC insertion between May 2002 and April 2012. Bivariate analyses were conducted relating physical and radiologic methods to predict RA placement of a CVC tip. A multiple logistic regression model was then derived, with all variables in the final model significant at p<0.05. Full and TC distance based models including linear discriminant analysis, classification and regression tree, bagging, random forest and support vector machine were developed and compared the area under ROC curve (AUROC), sensitivity and specificity using training and test data. Each modeling was conducted ten times. Results: Among 120 cases, 24 tips were placed in RA. Significant predictable factors were rib level, age and TC distance (all p-value <0.001). TC distance (p=0.001) was independently valid in multiple logistic regression. Both full and reducted models using TC distance in five classification methods showed high values of accuracy (above 88%) and AUROC (above 0.89). There were no statistically significant difference between full and reducted model in AUROC. The cut-off value for TC distance for detecting RA positioning of a CVC was 43.65 mm (sensitivity=83.3%, specificity=95.8%). Conclusions: TC distance is a useful marker for detecting RA positioning of a CVC.

Association of miR-34a, miR-130a, miR-150 and miR-155 polymorphisms with the risk of ischemic stroke

CHOI, GUN HO,KO, KI HAN,KIM, JUNG OH,KIM, JINKWON,OH, SEUNG HUN,HAN, IN BO,CHO, KYUNG GI,KIM, OK JOON,BAE, JINKUN,KIM, NAM KEUN Spandidos Publications 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.38 No.1

<P>MicroRNAs (miRNAs or miRs) are small (19-23 nt) non-coding RNA molecules that are endogenous regulators of gene expression. Previous studies have found that some miRNAs are related to the progression of ischemia in the cerebral artery. Furthermore, a recent study found a significant association between miRNA single nucleotide polymorphisms (SNPs) and the risk of ischemic stroke. Therefore, it may be valuable to investigate associations between megakaryocyte formation-related miRNA polymorphisms and the prevalence of ischemic stroke. We thus conducted a case-control study of 1,000 individuals who were screened for 4 miRNA polymorphisms (miR-34a rs6577555C>A, miR-130a rs731384C>T, miR-150 rs73056059G>A and miR-155 rs767649T>A) by PCR-RFLP analysis. The study population comprised 596 patients with ischemic stroke and 404 control subjects without any history of neurological disorders. We observed associations between miRNA polymorphisms and individual stroke subtypes. The miR-150 polymorphisms were significantly associated with ischemic stroke subgroups, such as left anterior descending artery (LAD) disease [GG vs. AA: adjusted odds ratio (AOR), 1.922; 95% confidence interval (CI), 1.003-3.681] and cardioembolism (GG vs. AA: AOR, 2.996; 95% CI, 1.293-6.939). Additionally, Cox proportional analysis indicated that the miR-150GA genotype was associated with survival in patients with ischemic stroke [adjusted hazard ratio (HR), 2.063; 95% CI, 1.142-3.727; P=0.017] and with the LAD subgroup [adjusted HR, 3.021; 95% CI, 1.345-6.785; P=0.008]. Our findings suggest that miR-150 polymorphisms may contribute to the development of ischemic stroke and may potentially act as biomarkers to predict the risk of ischemic stroke. To the best of our knowledge, this is the first study to evaluate the association between miRNA polymorphisms (miR-34aC>A, miR-130aC>T, miR-150G>A and miR-155T>A) and ischemic stroke.</P>