Association of Oral Contraceptives Use and Lung Cancer Risk among Women: an Updated Meta-analysis Based on Cohort and Case-control Studies

Wu, Wei,Yin, Zhi-Hua,Guan, Peng,Ren, Yang-Wu,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Background: Previous studies on the association of oral contraceptives (OC) use and lung cancer generated inconsistent findings. The aim of this study was to confirm any definite correlation between OC use and lung cancer risk. Methods: Publications were reviewed and obtained through PubMed and EMBASE databases literature search up to November, 2013. Reference lists from retrieved articles were also reviewed. The language of publication was restricted to English. A meta-analysis was performed to evaluate the association by calculating pooled odds ratios (ORs) and 95% confidence intervals (CIs). Results: A total of 14 studies consisting of 9 case-control studies and 5 cohort studies were finally included in this meta-analysis. There was no significant association observed between OC use and lung cancer risk in the overall analysis (OR=0.91; 95% CI=0.81-1.03). There was a significant protective effect in Europe (OR=0.74; 95% CI=0.60-0.91) and a borderline significant protective effect with an adenocarcinoma histology (OR=0.90; 95% CI=0.80-1.01) in subgroup analyses. No association was observed for methodological quality of study, study design, smoking status and case number of study. Conclusion: This meta-analysis suggests that OC use is not likely to be associated with the risk of lung cancer at all. While a significant protective effect of OC use on lung cancer was observed in Europe, interpretation should be cautious because of the potential biases of low-quality studies. At the same time, more attention should be paid to the possible association of OC use with adenocarcinoma of lung. Our findings require further research, with well-conducted and large-scale epidemiological studies to confirm effects of OC use on lung cancer.

NANOSCALE POROUS SILICON MICROCAVITY BIOSENSOR FOR NOVEL LABEL-FREE TUBERCULOSIS ANTIGEN–ANTIBODY DETECTION

BAO WU,GUOGUANG RONG,JUNWEI ZHAO,SHULIN ZHANG,YONGXIN ZHU,BOYONG HE 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.6

One third of the world population is estimated to have Mycobacterium tuberculosis infection. It is urgent to develop a rapid, inexpensive and convenient diagnostic method for detection of tuberculosis. Porous silicon material has taken more and more attention in recent years for biosensing applications and some useful results have been obtained. In this paper, we report the feasibility of applying porous silicon microcavity biosensor in a novel and relatively rapid serodiagnostic approach. Nowadays, most of serodiagnostic tests are based on labeled detection. Applying label-free detection methods can help develop fast and e±cient tuberculosis diagnostic tools, which can meet the current demand. In this study, we use this label-free sensing platform (i.e., porous silicon microcavity) to detect the interaction between 16 kDa antigen and anti-16 kDa antibody. Through a series of experiments, we verify the speci¯city and examine the sensitivity of this new diagnostic technique. The results show that it is feasible to apply porous silicon microcavity in the tests of tuberculosis.

Genipin-Crosslinked, Immunogen-Reduced Decellularized Porcine Liver Scaffold for Bioengineered Hepatic Tissue

Xiujuan Wu,Yujia Wang,Qiong Wu,Yi Li,Li Li,Jing Tang,Yujun Shi,Hong Bu,Ji Bao,Mingjun Xie 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.6

Liver disease affects millions of patients each year worldwide. Decellularized biologic matrices are plausible biomedical materials for bioengineered replacement hepatic tissue. However, one of the concerns for its safe medical application is the lack of objective assessment of the immunogen within the materials and in vivo immune responses to the matrices. The purpose of this study was to produce immunogen- reduced and biocompatible matrices from porcine liver. Whole porcine livers were perfusion decellularized and cross-linked with glutaraldehyde (GA) or genipin (GP). Proteins were extracted, and the migratory response of human leukocytes toward protein extracts was examined using an in vitro migration chamber. In addition, biopsy specimens of decellularized scaffolds were implanted subcutaneously into rodents to investigate scaffold immunogenicity. Histological staining confirmed cellular clearance from pig livers, with removal of nuclei and cytoskeletal components and widespread preservation of structural extracellular molecules. Polymerase chain reaction analysis showed that galactose-alpha-1,3-galactose-beta-1,4-N-acetylglucosamine (1,3 gal), swine leukocyte antigen, and porcine endogenous retrovirus were completely removed in the matrices. Decellularization significantly reduced the migration of monocytes compared with native porcine tissue. Although the proportion of transmigrating lymphocytes was much lower, repeating the cross-linking procedure reduced the migratory response. After implantation for 4 weeks, the decellularized and native samples were degraded, and the GA-treated group demonstrated a severe inflammatory reaction; however, minimal inflammatory cell infiltration was seen in the GPtreated group during the 8-week investigation period. In conclusion, our study provided evidence that GP crosslinking could significantly reduce the immunogenicity of decellularized liver biomaterials.

P53 Arg72Pro and MDM2 SNP309 Polymorphisms Cooperate to Increase Lung Adenocarcinoma Risk in Chinese Female Non-smokers: A Case Control Study

Ren, Yang-Wu,Yin, Zhi-Hua,Wan, Yan,Guan, Peng,Wu, Wei,Li, Xue-Lian,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Background: Cell cycle deregulation is a major component of carcinogenesis. The p53 tumor suppressor gene plays an important role in regulating cell cycle arrest, and mouse double minute 2 (MDM2) is a key regulator of p53 activity and degradation. Abnormal expression of p53 and MDM2 occurs in various cancers including lung cancer. Methods: We investigated the distribution of the p53 Arg72Pro (rs1042522) and MDM2 SNP309 (rs2279744) genotypes in patients and healthy control subjects to assess whether these single nucleotide polymorphisms (SNPs) are associated with an increased risk of lung adenocarcinomas in Chinese female non-smokers. Genotypes of 764 patients and 983 healthy controls were determined using the TaqMan SNP genotyping assay. Results: The p53 Pro/Pro genotype (adjusted OR = 1.55, 95% CI = 1.17-2.06) significantly correlated with an increased risk of lung adenocarcinoma, compared with the Arg/Arg genotype. An increased risk was also noted for MDM2 GG genotype (adjusted OR = 1.68, 95% CI = 1.27-2.21) compared with the TT genotype. Combined p53 Pro/Pro and MDM2 GG genotypes (adjusted OR = 2.66, 95% CI = 1.54-4.60) had a supermultiplicative interaction with respect to lung adenocarcinoma risk. We also found that cooking oil fumes, fuel smoke, and passive smoking may increase the risk of lung adenocarcinomas in Chinese female non-smokers who carry p53 or MDM2 mutant alleles. Conclusions: P53 Arg72Pro and MDM2 SNP309 polymorphisms, either alone or in combination, are associated with an increased lung adenocarcinoma risk in Chinese female non-smokers.

Association analysis of the SNP (rs345476947) in the FUT2 gene with the production and reproductive traits in pigs

Haifei Wang,Sen Wu,Jiayun Wu,Shouyong Sun,Shenglong Wu,Wen Bin Bao 한국유전학회 2018 Genes & Genomics Vol.40 No.2

The FUT2 gene was considered as an important candidate for pathogenic infections, while the potential associations between this gene and the production and reproductive traits of pigs have not been explored. In this study, we detected the genetic variants of porcine FUT2 gene and analyzed the associations of the polymorphisms with FUT2 mRNA expression and production and reproductive traits (age at 100 kg, backfat thickness at 100 kg, eye muscle thickness, the number of newborn piglets, the number of weaned piglets, and birth weight) in 100 Large White sows. One single nucleotide polymorphism (SNP) (rs345476947, C→T) in the intron of FUT2 and three genotypes (TT, CT and CC) were determined. Association analysis revealed significant associations between this SNP with the number of newborn piglets and weaned piglets. Furthermore, individuals with the TT genotype had significantly higher numbers of newborn piglets and weaned piglets than those with the CC genotype (P < 0.05). Quantitative PCR analysis showed that FUT2 expression in individuals with CC genotype was significantly higher than those with TT and CT genotypes in the liver and lymph gland (P < 0.05) and higher than that of CT in the spleen, kidney, and duodenum (P < 0.05). These findings indicated that the TT genotype may be a favorable genotype for the reproductive traits of pigs. Our study revealed the genetic variants of the FUT2 gene and identified a promising candidate SNP (rs345476947) associated with the reproductive traits, which has the potential to be applied in selective breeding of pigs.

Design-Oriented Stability of Outer Voltage Loop in Capacitor Current Controlled Buck Converters

Zhang, Xi,Zhang, Zhongwei,Bao, Bocheng,Bao, Han,Wu, Zhimin,Yao, Kaiwen,Wu, Jing The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4

Due to the inherent feedforward of load current, capacitor current (CC) control shows a fast transient response that makes it suitable for the power supplies used in various portable electronic devices. However, considering the effect of the outer voltage loop, the stable range of the duty-cycle is significantly diminished in CC controlled buck converters. To investigate the stability effect of the outer voltage loop on buck converters, a CC controlled buck converter with a proportion-integral (PI) compensator is taken as an example, and its second-order discrete-time model is established. Based on this model, the instability caused by the duty-cycle is discussed with consideration of the outer voltage loop. Then the dynamical effects of the feedback gain of the PI compensator and the equivalent series resistance (ESR) of the output capacitor on the CC controlled buck converter with a PI compensator are studied. Furthermore, the design-oriented closed-loop stability criterion is derived. Finally, PSIM simulations and experimental results are supplied to verify the theoretical analyses.

Design-Oriented Stability of Outer Voltage Loop in Capacitor Current Controlled Buck Converters

Xi Zhang,Zhongwei Zhang,Bocheng Bao,Han Bao,Zhimin Wu,Kaiwen Yao,Jing Wu 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4

Due to the inherent feedforward of load current, capacitor current (CC) control shows a fast transient response that makes itsuitable for the power supplies used in various portable electronic devices. However, considering the effect of the outer voltageloop, the stable range of the duty-cycle is significantly diminished in CC controlled buck converters. To investigate the stabilityeffect of the outer voltage loop on buck converters, a CC controlled buck converter with a proportion-integral (PI) compensatoris taken as an example, and its second-order discrete-time model is established. Based on this model, the instability caused by theduty-cycle is discussed with consideration of the outer voltage loop. Then the dynamical effects of the feedback gain of the PIcompensator and the equivalent series resistance (ESR) of the output capacitor on the CC controlled buck converter with a PIcompensator are studied. Furthermore, the design-oriented closed-loop stability criterion is derived. Finally, PSIM simulationsand experimental results are supplied to verify the theoretical analyses.

Roles of microRNA-206 in Osteosarcoma Pathogenesis and Progression

Bao, Yun-Ping,Yi, Yang,Peng, Li-Lin,Fang, Jing,Liu, Ke-Bin,Li, Wu-Zhou,Luo, Hua-Song Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Backgroud and Aims: MicroRNA-206 has proven to be down-regulated in many human malignancies in correlation with tumour progression. Our study aimed to characterize miR-206 contributions to initiation and malignant progression of human osteosarcoma. Methods: MiR-206 expression was detected in human osteosarcoma cell 1ine MG63, human normal osteoblastic cell line hFOB 1.19, and paired osteosarcoma and normal adjacent tissues from 65 patients using quantitative RT-PCR. Relationships of miR-206 levels to clinicopathological characteristics were also investigated. Moreover, miR-206 mimics and negative control siRNA were transfected into MG63 cells to observe effects on cell viability, apoptosis, invasion and migration. Results: We found that miR-206 was down-regulated in the osteosarcoma cell line MG63 and primary tumor samples, and decreased miR-206 expression was significantly associated with advanced clinical stage, T classification, metastasis and poor histological differentiation. Additionally, transfection of miR-206 mimics could reduce MG-63 cell viability, promote cell apoptosis, and inhibit cell invasion and migration. Conclusions: These findings indicate that miR-206 may have a key role in osteosarcoma pathogenesis and development. It could serve as a useful biomarker for prediction of osteosarcoma progression, and provide a potential target for gene therapy.

Association Between Three eNOS Polymorphisms and Cancer Risk: a Meta-analysis

Wu, Xun,Wang, Zhi-Feng,Xu, Yin,Ren, Rui,Heng, Bao-Li,Su, Ze-Xuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene may influence the risk of cancer, but the results are still debatable. Therefore, we performed a systematic review to provide a more complete picture and conducted a meta-analysis to derive a precise estimation. We searched PubMed, EMBASE, EBSCO, Google Scholar and China National Knowledge Infrastructure (CNKI) databases until April 2014 to identify eligible studies. Thirty-one studies with cancer patients and controls were included in the meta-analysis. Overall, the polled analysis revealed that the T-786C polymorphism was significantly associated with increased cancer risk under multiple genetic models (C vs T: OR=1.135, 95%CI=1.048-1.228; CC vs TT: OR=1.278, 95%CI=1.045-1.562; TC vsTT: OR=1.136, 95%CI=1.023-1.261; CC+TC vs TT: OR=1.159, 95%CI=1.047-1.281; CC vs TC+TT: OR=1.204, 95%CI= 1.003-1.447). G894T was associated with significant risk for females (TT vs GG: OR=1.414, 95%CI=1.056-1.892; TT vs GT+GG: OR=1.356, 95%CI=1.108-1.661) and for breast cancer (T vs G: OR=1.097, 95%CI=1.001-1.203; TT vs GG: OR=1.346, 95%CI=1.012-1.789; TT vs GT+GG: OR=1.269, 95%CI=1.028-1.566). Increased susceptibility was revealed for prostate cancer with 4a/b (ba vs bb: OR=1.338, 95%CI=1.013-1.768; aa+ba vs bb: OR=1.474, 95%CI=1.002-2.170). This meta-analysis indicated that the eNOS T-786C polymorphism is associated with elevated cancer risk; the G894T polymorphism contributes to susceptibility to breast cancer and cancer generally in females; and the 4a/b polymorphism may be associated with prostate cancer risk.

Molecular Characterization and Expression Analysis of S6K1 in Cashmere Goats (Capra hircus)

Wu, Manlin,Bao, Wenlei,Hao, Xiyan,Zheng, Xu,Wang, Yanfeng,Wang, Zhigang Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.8

p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession GU144017) and its 3' noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3' noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.