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- 저자 : Alireza Sima (검색결과 4 건)
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Sazegari Sima,Niazi Ali,Shahriari-Ahmadi Farajollah,Afsharifar Alireza 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5
Terpenoid indole alkaloids (TIA) are important pharmaceuticals produced in low amounts through the terpenoid indole alkaloid pathway in Catharanthus roseus. Production of these alkaloids is controlled by biosynthetic and regulatory genes. CrMYC1 is a chief transcription factor that regulates terpenoid indole alkaloid production. In this study, we cloned CrMYC1 and overexpressed it in C. roseus hairy roots to investigate its effect on the production of the TIAs ajmalicine and catharan- thine. qRT-PCR results revealed that CrMYC1 expression in transgenic CrMYC1-overexpressing hairy root lines was 3–6 times higher than that of the wild-type hairy root control line. Also, we detected a significant increase in strictosidine β-D- glucosidase (SGD) expression, a critical enzyme in TIA biosynthesis, in the overexpression lines. HPLC analysis showed that CrMYC1 overexpression significantly improved the accumulation of ajmalicine (13 to 14-fold) and catharanthine (3 to 4.4-fold) compared to the control hairy root line. These results provide insight into the role of this understudied transcription factor in TIA pathway regulation and emphasize further research objectives.
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Shiva Torabian,Alireza Soffianian,Sima Fakheran,Ali Asgarian,Hossein Akbari Feizabad,Josef Senn 대한공간정보학회 2018 Spatial Information Research Vol.26 No.1
One of the primary reason of species extinction especially rare species with very specific requirements, is habitat destruction. To protect these species, habitat suitability evaluation plays a central role. Hence, an attempt is made in this study to evaluate the suitability of sand cat’s habitat in a sand dune-dominated landscape in Iran. Four Landsat-derived indices including Normalized Difference Vegetation Index (NDVI), Weighted Difference Vegetation Index, Brightness Index (BI) and Salinity Index were combined to characterize sand cat’s habitat requirements through a land use land cover (LULC) map. Furthermore, a set of landscape metrics were employed to explore the spatial pattern LULC classes. Sand cat’s habitat suitability map was generated by linear combination of the standardized and relatively weighted NDVI and BI indices and then categorized into five classes of most suitable, highly suitable, moderately suitable, least suitable and not suitable. The results showed that about 75% of the total area is suitable for sand cat. Although this region is rich in biodiversity, it has not yet been subject to any conservation planning and should be granted more conservation attentions.
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Masoud M Malekzadeh,Alireza Sima,Sudabeh Alatab,Anahita Sadeghi,Nasser Ebrahimi Daryani,Payman Adibi,Iradj Maleki,Hassan Vossoughinia,Hafez Fakheri,Abbas Yazdanbod,Seyed Alireza Taghavi,Rahim Aghazade 대한장연구학회 2019 Intestinal Research Vol.17 No.3
Background/Aims: A recent study revealed increasing incidence and prevalence of inflammatory bowel disease (IBD) in Iran. The Iranian Registry of Crohn’s and Colitis (IRCC) was designed recently to answer the needs. We reported the design, methods of data collection, and aims of IRCC in this paper. Methods: IRCC is a multicenter prospective registry, which is established with collaboration of more than 100 gastroenterologists from different provinces of Iran. Minimum data set for IRCC was defined according to an international consensus on standard set of outcomes for IBD. A pilot feasibility study was performed on 553 IBD patients with a web-based questionnaire. The reliability of questionnaire evaluated by Cronbach’s α. Results: All sections of questionnaire had Cronbach’s α of more than 0.6. In pilot study, 312 of participants (56.4%) were male and mean age was 38 years (standard deviation=12.8) and 378 patients (68.35%) had ulcerative colitis, 303 subjects (54,7%) had college education and 358 patients (64.74%) were of Fars ethnicity. We found that 68 (12.3%), 44 (7.9%), and 13 (2.3%) of participants were smokers, hookah and opium users, respectively. History of appendectomy was reported in 58 of patients (10.48%). The most common medication was 5-aminosalicylate (94.39%). Conclusions: To the best of our knowledge, IRCC is the first national IBD registry in the Middle East and could become a reliable infrastructure for national and international research on IBD. IRCC will improve the quality of care of IBD patients and provide national information for policy makers to better plan for controlling IBD in Iran.
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Antitumor Effect of IP-10 by Using two Different Approaches: Live Delivery System and Gene Therapy
Yasaman Taslimi,Farnaz Zahedifard,Sima Habibzadeh,Tahereh Taheri,Hossain Abbaspour,Alireza Sadeghipour,Elham Mohit,Sima Rafati 한국유방암학회 2016 Journal of breast cancer Vol.19 No.1
Purpose: Immunotherapy is one of the treatment strategies for breast cancer, the most common cancer in women worldwide. In this approach, the patient’s immune system is stimulated to attack microscopic tumors and control metastasis. Here, we used interferon γ-induced protein 10 (IP-10), which induces and strengthens antitumor immunity, as an immunotherapeutic agent. We employed Leishmania tarentolae, a nonpathogenic lizard parasite that lacks the ability to persist in mammalian macrophages, was used as a live delivery system for carrying the immunotherapeutic agent. It has been already shown that arginase activity, and consequently, polyamine production, are associated with tumor progression. Methods: A live delivery system was constructed by stable transfection of pLEXSY plasmid containing the IP-10-enhanced green fluorescent protein (IP-10- egfp) fusion gene into L. tarentolae. Then, the presence of the IP-10-egfp gene and the accurate integration location into the parasite genome were confirmed. The therapeutic efficacy of IP- 10 delivered via L. tarentolae and recombinant pcDNA-(IP- 10-egfp) plasmid was compared by determining the arginase activity in a mouse 4T1 breast cancer model. Results: The pcDNA- (IP-10-egfp) group showed a significant reduction in tumor weight and growth. Histological evaluation also revealed that only this group demonstrated inhibition of metastasis to the lung tissue. The arginase activity in the tissue of the pcDNA-(IP- 10-egfp) mice significantly decreased in comparison with that in normal mice. No significant difference was observed in arginase activity in the sera of mice receiving other therapeutic strategies. Conclusion: Our data indicates that IP-10 immunotherapy is a promising strategy for breast cancer treatment, as shown in the 4T1-implanted BALB/c mouse model. However, the L. tarentolae- (IP-10-EGFP) live delivery system requires dose modifications to achieve efficacy in the applied regimen (six injections in 3 weeks). Our results indicate that the arginase assay could be a good biomarker to differentiate tumoral tissues from the normal ones.
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