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        선박평형수 처리장치의 Scale-up을 위한 조건 최적화 연구

        강아영 ( Ahyoung Kang ),송주영 ( Ju Yeong Song ) 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.5

        선행연구에서는 2차오염이 없는 물리적 처리방법인 전단응력을 이용한 선박평형수 처리장치에 대하여 연구하였다. 정지상태의 외부 실린더와 회전하는 내부 실린더 사이에 발생하는 전단응력을 이용하여 다양한 미생물을 파쇄 처리하는 원리이며, 전단응력은 실린더 표면의 형태나 회전속도에 따라 크기가 달라진다. 전단응력의 크기와 전단응력을 받는 시간에 따라 멸균정도가 차이를 보이기 때문에, 다양한 조건에 따른 멸균효율을 밝히는 실험이 필요하며 이를 통해 상업용 장치의 제작을 위한 기초 자료를 마련하고자 하였다. 선행연구에서는 disk type과 cylinder type의 비교를 통하여 cylinder type이 우수함을 밝혔다. 본 연구에서는 선행연구를 통하여 얻은 결과를 바탕으로 cylinder type, groove type, knurling type의 표면 조건을 변화시켜 가장 우수한 조합을 밝혀냄과 동시에 실린더 사이의 간격에 따른 회전속도 및 유속 조건을 최적화 하고자하였다. 그 결과 groove type에서 250 mL/min의 유량조건에서는 8000 rpm 이상, 혹은 500mL/min의 유량조건에서는 10000 rpm 이상에서 100% 살균 처리되는 결과를 얻었다. In the prior work, we studied a ballast water treatment apparatus, which is secondary pollution free by using physical treatment of shear stress. The principle of this apparatus is smashing various microorganisms by shear stress generated between stationary outer cylinder and revolving inner cylinder. Because of various magnitude of shear stress according to the inner cylinder surface type and revolution speed, an appropriate surface type and optimum revolution speed should be studied by consecutive experiment to determine the reference data for commercial apparatus. Through a comparative study of disk type and cylinder type of ballast water treatment apparatus, cylinder type is turned out to be superb to disk type. In this study, we studied to determine the superior collaboration of cylinder type, groove type and knurling type of inner cylinder to non patterned outer cylinder, and to optimize the revolution speed and flow rate according to the gap between inner cylinder and outer cylinder. As a result, we could get perfect sterilization effect at groove type under the conditions of 250 mL/min of flow rate at 8,000 rpm and 500 mL/min of flow rate at 10,000 rpm respectively.

      • <i>Nibrella saemangeumensis</i> gen. nov., sp. nov. and <i>Nibrella viscosa</i> sp. nov., novel members of the family <i>Cytophagaceae</i>, isolated from seawater

        Kang, Ji Young,Chun, Jeesun,Choi, Ahyoung,Cho, Jang-Cheon,Jahng, Kwang Yeop International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.12

        <P>Two strains, designated GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP>, were isolated from seawater of the Saemangeum Embankment in Jeollabuk-do, Korea. The cells of the two strains were Gram-reaction-negative and non-motile, and formed multicellular filaments. The colonies of the two strains were pink-pigmented and able to grow at 15–37 °C (optimum 25 °C) on R2A and NA medium. Strains GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> grew at pH 6.5–10 (optimum pH 7.5) and pH 5.5–9.5 (optimum pH 7.5), and within NaCl ranges of 0–0.4 % and 0–1 %, respectively. The polar lipid profiles of the two strains contained phosphatidylethanolamine, five unknown aminolipids, an unknown phospholipid and four or five unknown lipids. The DNA G+C contents of strains GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> were 56.0 and 54.5 mol%, respectively. The respiratory quinone detected in both strains was MK-7. The 16S rRNA gene sequence similarity between GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> was 95.5 %. The 16S rRNA gene sequence similarities of the two strains to closely related reference strains were less than 89 %. Phylogenetic analysis based on 16S rRNA genes showed that GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> formed a distinct phyletic line in the family <I>Cytophagaceae</I>. On the basis of the phenotypic, chemotaxonomic and phylogenetic properties, strains GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> represent two novel species in a new genus within the family <I>Cytophagaceae</I>, for which the names <I>Nibrella saemangeumensis</I> gen. nov., sp. nov. and <I>Nibrella viscosa</I> sp. nov. are proposed. The type strain of <I>Nibrella saemangeumensis</I> is GCR0103<SUP>T</SUP> ( = KACC 16453<SUP>T</SUP> = JCM 17927<SUP>T</SUP>) and the type strain of <I>Nibrella viscosa</I> is GYR3121<SUP>T</SUP> ( = KACC 16447<SUP>T</SUP> = JCM 17925<SUP>T</SUP>).</P>

      • <i>Hymenobacter koreensis</i> sp. nov. and <i>Hymenobacter saemangeumensis</i> sp. nov., isolated from estuarine water

        Kang, Ji Young,Chun, Jeesun,Choi, Ahyoung,Moon, Sung Hyun,Cho, Jang-Cheon,Jahng, Kwang Yeop International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.12

        <P>Two Gram-reaction-negative, rod-shaped, non-motile and red–pink-pigmented bacterial strains, designated GYR3077<SUP>T</SUP> and GSR0100<SUP>T</SUP>, were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077<SUP>T</SUP> and GSR0100<SUP>T</SUP> exhibited sequence similarities of 95.9 % to <I>Hymenobacter deserti</I> ZLB-3<SUP>T</SUP> and 96.6 % to <I>Hymenobacter soli</I> PB17<SUP>T</SUP>, respectively, and indicated that these isolates belonged to the phylum <I>Bacteroidetes</I>. The major cellular fatty acids present in the two isolates were iso-C<SUB>15 : 0</SUB>, C<SUB>16 : 1</SUB>ω5<I>c</I>, summed feature 4 (iso-C<SUB>17 : 1</SUB> I and/or anteiso-C<SUB>17 : 1</SUB> B) and summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I> and/or C<SUB>16 : 1</SUB>ω6<I>c</I>). The major respiratory quinone and polyamine patterns were menaquinone-7 and <I>sym</I>-homospermidine, characteristic of the genus <I>Hymenobacter</I>. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077<SUP>T</SUP> and GSR0100<SUP>T</SUP> were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077<SUP>T</SUP> and GSR0100<SUP>T</SUP> was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077<SUP>T</SUP> and GSR0100<SUP>T</SUP> were considered to represent two novel species of the genus <I>Hymenobacter</I>, for which the names <I>Hymenobacter koreensis</I> sp. nov. (type strain GYR3077<SUP>T</SUP> = KACC 16451<SUP>T</SUP> = JCM 17924<SUP>T</SUP>) and <I>Hymenobacter saemangeumensis</I> sp. nov. (type strain GSR0100<SUP>T</SUP> = KACC 16452<SUP>T</SUP> = JCM 17923<SUP>T</SUP>) are proposed.</P>

      • <i>Celeribacter marinus</i> sp. nov., isolated from coastal seawater

        Baek, Kiwoon,Choi, Ahyoung,Kang, Ilnam,Cho, Jang-Cheon International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.4

        <P>A Gram-staining-negative, non-motile, non-pigmented and rod-shaped bacterial strain, designated IMCC12053<SUP>T</SUP>, was isolated from coastal surface seawater of the Yellow Sea, Korea. Optimal growth of strain IMCC12053<SUP>T</SUP> was observed at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. 16S rRNA gene sequence comparisons showed that strain IMCC12053<SUP>T</SUP> was most closely related to <I>Celeribacter baekdonensis</I> L-6<SUP>T</SUP> (97.5 % similarity) and <I>Celeribacter neptunius</I> H 14<SUP>T</SUP> (96.0 %). Strain IMCC12053<SUP>T</SUP> formed a robust phylogenetic clade with members of the genus <I>Celeribacter</I>. The DNA–DNA relatedness value between IMCC12053<SUP>T</SUP> and <I>C. baekdonensis</I> was far lower than 70 % (35.7–42.5 %), which indicated that strain IMCC12053<SUP>T</SUP> is a novel genomic species of the genus <I>Celeribacter</I>. The major respiratory isoprenoid quinone was ubiquinone-10 (Q-10) and major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and aminolipids. The DNA G+C content was 61.0 mol%. On the basis of genotypic and phenotypic data collected in this study, it is proposed that strain IMCC12053<SUP>T</SUP> represents a novel species of the genus <I>Celeribacter</I>, for which the name <I>Celeribacter marinus</I> sp. nov. is proposed. The type strain is IMCC12053<SUP>T</SUP> ( = KACC 17482<SUP>T</SUP> = NBRC 109702<SUP>T</SUP>).</P>

      • <i>Granulosicoccus marinus</i> sp. nov., isolated from Antarctic seawater, and emended description of the genus <i>Granulosicoccus</i>

        Baek, Kiwoon,Choi, Ahyoung,Kang, Ilnam,Im, Mihye,Cho, Jang-Cheon International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.12

        <P>A Gram-staining-negative, motile by flagella, non-pigmented, poly-β-hydroxybutyrate-producing, strictly aerobic and sphere-shaped bacterium, IMCC3490<SUP>T</SUP>, was isolated from a coastal seawater sample from the Antarctic Peninsula. Optimal growth of strain IMCC3490<SUP>T</SUP> was observed at 20 °C, pH 8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain IMCC3490<SUP>T</SUP> belonged to the genus <I>Granulosicoccus</I> in the family <I>Granulosicoccaceae</I>. The strain was closely related to <I>Granulosicoccus antarcticus</I> IMCC3135<SUP>T</SUP> (98.8 % 16S rRNA gene sequence similarity) and <I>Granulosicoccus coccoides</I> Z 271<SUP>T</SUP> (98.5 %). The DNA–DNA relatedness values between IMCC3490<SUP>T</SUP> and type strains of the two species of the genus were far lower than 70 %, which indicated strain IMCC3490<SUP>T</SUP> is a novel genomic species of the genus <I>Granulosicoccus</I>. The major fatty acids of strain IMCC3490<SUP>T</SUP> were summed feature 8 (C<SUB>18 : 1</SUB>ω7<I>c</I> and/or C<SUB>18 : 1</SUB>ω6<I>c</I>) and summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I> and/or C<SUB>16 : 1</SUB>ω6<I>c</I>). The isoprenoid quinone detected was ubiquinone-8 (Q-8) and predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 61.0 mol%. On the basis of phylogenetic analyses, DNA–DNA relatedness values and phenotypic data, it is suggested that strain IMCC3490<SUP>T</SUP> represents a novel species of the genus <I>Granulosicoccus</I>, for which the name <I>Granulosicoccus marinus</I> sp. nov. is proposed. The type strain is IMCC3490<SUP>T</SUP> ( = KACC 17483<SUP>T</SUP> = NBRC 109704<SUP>T</SUP>). An emended description of the genus <I>Granulosicoccus</I> is also provided.</P>

      • <i>Kordia</i> <i>antarctica</i> sp. nov., isolated from Antarctic seawater

        Baek, Kiwoon,Choi, Ahyoung,Kang, Ilnam,Lee, Kiyoung,Cho, Jang-Cheon International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.10

        <P>A Gram-staining-negative, chemoheterotrophic, yellow-pigmented, non-motile, flexirubin-negative, facultatively anaerobic bacterium, designated strain IMCC3317<SUP>T</SUP>, was isolated from a coastal seawater sample from the Antarctic Penninsula. Optimal growth of strain IMCC3317<SUP>T</SUP> was observed at 20 °C, pH 8.0 and in the presence of 2–3 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC3317<SUP>T</SUP> belonged to the genus <I>Kordia</I> and was closely related to <I>Kordia algicida</I> OT-1<SUP>T</SUP> (96.7 % sequence similarity) and <I>Kordia periserrulae</I> IMCC1412<SUP>T</SUP> (96.1 % sequence similarity). The major fatty acids were 10-methyl C<SUB>16 : 0</SUB> and/or iso-C<SUB>16 : 1</SUB>ω9<I>c</I>, iso-C<SUB>17 : 0</SUB> 3-OH, iso-C<SUB>15 : 0</SUB> and anteiso-C<SUB>15 : 0</SUB>. The G+C content of the genomic DNA was 35.1 mol%. The strain contained menaquinone-6 (MK-6) as the respiratory quinone. The polar lipids detected in the strain were phosphatidylethanolamine and unknown aminophospholipids, aminolipids and polar lipids. On the basis of phylogenetic distinction and differential phenotypic characteristics, it is suggested that strain IMCC3317<SUP>T</SUP> ( = KCTC 32292<SUP>T</SUP> = NBRC 109401<SUP>T</SUP>) be assigned to the genus <I>Kordia</I> as the type strain of a novel species, for which the name <I>Kordia</I> <I>antarctica</I> sp. nov. is proposed.</P>

      • Highly efficient catalytic systems based on Pd-coated microbeads

        Lim, Jin Hyun,Cho, Ahyoung,Lee, Seung Hwan,Park, Bumkyo,Kang, Dong Woo,Koo, Chong Min,Yu, Taekyung,Park, Bum Jun Elsevier 2018 APPLIED SURFACE SCIENCE - Vol.429 No.-

        <P><B>Abstract</B></P> <P>The efficiency of two prototype catalysis systems using palladium (Pd)-coated microparticles was investigated with regard to the recovery and recyclability of the catalytic particles. One such system was the interface-adsorption method, in which polymer particles coated with Pd nanoparticles strongly and irreversibly attach to the oil-water interface. Due to the irreversible adsorption of the catalytic particles to the interface, particle loss was completely prevented while mixing the aqueous solution and while collecting the products. The other system was based on the magnetic field-associated particle recovery method. The use of polymeric microparticles containing Pd nanoparticles and magnetite nanoparticles accelerated the sedimentation of the particles in the aqueous phase by applying a strong magnetic field, consequently suppressing drainage of the particles from the reactor along the product stream. Upon multiple runs of the catalytic reactions, it was found that conversion does not change significantly, demonstrating the excellent recyclability and performance efficiency in the catalytic processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pd-coated PS particles irreversibly attached to the oil-water interface. </LI> <LI> Particle adsorption to the interface improved recyclability of the catalytic particles. </LI> <LI> Pd-MNP-PS particles were efficiently responsive under magnetic field. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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