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Raphanus sativus Var. Chungpihongsim의 hairy root 배양에 의한 anthocyanin 생성
황성진,백윤웅,고경민,안준철,황백 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-
청피홍심무우를 재료로 A. rhizogenes A₄균주를 접종하여 hairy root를 유도하였으며 유도된 hairy root는 paper electrophoresis를 통하여 mannopine과 agropine이 검출되어 형질전환 되었음을 확인하였다. Hairy root는 MS의 기본염을 1/2로 희석시킨 1/2 MS, sucrose 3%, pH 5.2로 조성된 배지에서 최적인 성장을 보였다. 유도된 hairy root는 각 strain사이에 색소능에 다소간의 차이를 보였으며 2, 4-D kinetin의 적절한 조합의 처리구에서는 탈분화와 더불어 색소가 형성되었고, 그 중 2, 4-D와 kinetin을 각각 1 ppm, 5 ppm 처리하였을 때 최대 색소능을 보였다. hairy root의 탈분화시 형성되는 색소는 원 식물 뿌리의 주 색소인 pelargonidin 계열의 색소로 추정되며 총 색소량은 49mg/100g(F.W.)로 잠정 계산되었다. Hairy root induced following inoculation of the root disc and plantlet fragment of R. sativus Var Chungpihongsim with A. rhizogenes A₄. The hairy root contained agropine and mannopine. Medium contained 1/2 MS salt, sucrose 3%, pH 5.2 was optimized to growth of hairy root. Depending on the hairy root line, the level of pigment in hairy root varied somewhat . When 2, 4-D and kinetin were added in hairy root, the synthesis of pigment induced with deorganization of hairy root. Especially, addition of 2, 4-D 1 ppm and kinetin 5 ppm showed the maximum synthesis of pigment. Compared with anthocyanin extract of mature root three pigments were assumed to acylated pelargonin. The content of anthocyanin in these sample was 49mg/100(F.W.).
Ahn, Jun-Cheul,Chong, Won-Seog,Kim, Young-Soon,Hwang, Baik The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.2
Saikosaponin productivity was examined in a Bupleurum falcatum L. BFHR2 hairy root culture in response to changes in the sucrose content $(2{\sim}8%)$, nitrogen content $(0{\sim}250mM\;NH_4NO_3)$, phosphate content $(0{\sim}12mM\;NaH_2PO_4)$, and the potassium content $(0{\sim}87.2 mM\; KCl)$ of the culture media. We found that the conditions for maximal saikosaponin production differed from those for optimal root growth. Highest saikosaponin yield was achieved for 8% sucrose, 62mM $NH_4NO_3$ 1.2 mM $NaH_2PO_4$, and 0.5mM KCl.
Ahn, Jun Cheul,Kim, Sung Ho,Kim, Min Young,Kim, Ok Tae,Kim, Kwang Soo,Hwang, Baik 한국식물학회 2003 Journal of Plant Biology Vol.46 No.1
We studied fluctuations in the production of Hwangchil lacquer and major essential oils by Dendropanax morbifera. Le'v. Considerable seasonal as well as intrespeciffic (individual-tree) variations were observed. Yields of Hwangchil lasquer as well as β-elemene, α-selinene, β-selinene, germacrene D, and δ-cadinene also depended on harvesting time, with levels being generally higher in July and August.
미치광이풀 ( Scopolia parviflora ) 의 모상근 배양에 의한 Tropane Alkaloid 생산
안준철(Jun Cheul Ahn),정병균(Byung Gyun Jung),백윤웅(Yun Woong Paek),김영준(Young Jun Kim),고경민(Kyeong Min Ko),황성진(Sung Jin Hwang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.3
Transformed hairy roots of Scopolia parviflora, prducing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A_4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaloid content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their mass spectra data with those of authentic compounds.
도라지 ( Platycodon grandiflorum ) 뿌리조직에서 고농도 Polyacetylene 함유 모상근의 (毛狀根) 유도 및 배양
안준철 ( Jun Cheul Ahn ),소상섭 ( Sang Seop So ),( Koichiro Shimomura ),황백 ( Baik Hwang ) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.4
Hairy roots of Korean balloon flower (Platycodon grandiflorum A. DC) were induced from the root tissues infected with Agrobacterium rhizogenes ATCC 15834. Growth and polyacetylene [lobetyol (1), lobetyolin (2) and lobetyolinin (3)] production of ten hairy root clones cultured in 1/4 Gamborg B5 (B5) liquid medium were determined. One selected hairy root clone (D6) grew well in hormone free-B5 liquid medium and showed maximum content of polyacetylenes at week 6 for 1 (0.375% dry wt) and at week 7 for 2 and 3 (3.030% and 0.206% dry wt, respectively) whose levels were much higher than those of the intact plant root (1: 0.019%, 2: 0.077% dry wt, 3 was not detected).
청피홍심무우 ( Raphanus sativus cv. Chungpihongsim ) 의 모상근 배양에 의한 안토시아닌 생성
안준철(Jun Cheul Ahn),백윤웅(Yun Woung Paek),강영희(Young Hee Kang),황백(Baik Hwang) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1
The hairy root culture of Raphanus sativus cv. Chungpihongsim was established by transformation with Agrobacterium rhizogenes A_4. The transformed roots grew well in adjusted Murashige and Skoog medium to 1/2 basal salts, pH5.2,3% sucrose. Agropine and mannopine, poine synthesized in the transformed tissue were detected in the extract of hairy roots. When 2,4-D and kinetin were added in culture medium of hairy roots, the synthesis of anthocyanin was induced with disorganization of hairy root. Especially, addition of 0.45 μM 2,4-D and 2.3 μM kinetin showed the maximum synthesis of anthocyanin. Pattern of anthocyanin synthesized in transformed roots was somewhat different from that of ordinary roots. However, aglycone part of all anthocyanin was identified as pelargonidin. The content of total anthocyanin in this sample was tentatively calculated 0.49 mg/g fresh weight.
시호 ( Bupleurum falcatum L. ) 의 모상근 배양을 통한 Saponin 생산 - 1. 캘러스 , 부정근 , 모상근과 재배근의 Saponin 양상과 함량 비교
안준철(Jun Cheul Ahn),백윤웅(Yun Woong Paek),성충기(Chung Ki Sung),강근형(Geun Hyung Kang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from thses were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 ㎛ 2,4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes A_4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.