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      • KCI등재

        레몬과 크랜베리즙이 닭 다리육의 저장품질에 미치는 영향

        김동욱(Dongwook Kim),김희진(Hee-Jin Kim),김혜진(Hye-Jin Kim),김정수(Jung-Soo Kim),김한나(Hanna Kim),Joko Sujiwo,강석원(Seokwon Kang),곽현아(Hyeon-Ah Gwak),장애라(Aera Jang) 한국가금학회 2018 韓國家禽學會誌 Vol.45 No.1

        본 연구는 레몬즙과 크랜베리즙에 닭 다리육을 침지하여 저장성 증진 및 품질에 미치는 영향을 규명하고자 실시하였다. 처리구는 닭 다리육을 멸균수에 침지한 대조구(CON), 1% 레몬즙에 침지한 처리구(LJ), 1% 크랜베리즙에 침지한 처리구(CJ), 0.5% 레몬즙과 0.5% 크랜베리즙을 혼합하여 침지한 처리구(LCJ)이었다. 닭 다리육을 레몬즙과 크랜베리즙에 20분간 침지시킨 후 4±1℃ 냉장실에서 0, 3, 6, 9일 동안 저장하면서 pH, 총균수, 육색, 관능적 특성, 지방산패도, 휘발성 염기태질소, 전자코를 이용하여 향기패턴의 변화를 분석하였다. 저장 3일차부터 모든 처리구는 대조구보다 유의적으로(P<0.05) 낮은 총균수를 나타내었으며, 또한 종합적 기호도는 저장 3일차부터 대조구보다 유의적으로(P<0.05) 높은 점수를 나타내었다. 또한 지방산패도와 휘발성 염기태질소 함량도 저장 3일차부터 처리구가 대조구보다 유의적으로 낮은 값을 나타내어 레몬즙과 크랜베리즙의 처리가 저장기간 동안 닭 다리육의 부패를 억제시킨 것으로 사료되며 이는 저장 3일차부터 처리구의 분포가 대조구와 구분되는 전자코 향기분석 결과와 유사하였다. 결과적으로 1% 천연 레몬즙과 크랜베리즙의 처리는 저장기간 동안 닭 다리육의 지방 산패와 단백질 부패를 억제하여 관능적인 특성의 감소를 지연시켜 저장 품질을 향상시키는 것으로 사료된다. This study was performed to evaluate the effect of lemon and cranberry juice on meat quality of chicken thighs during cold storage. Experimental groups were chicken thigh meat dipped into distilled water (CON), 1% lemon juice (LJ), 1% cranberry juice (CJ), and a mixture of 0.5% lemon juice and 0.5% cranberry juice (LCJ). The meat quality traits were determined at day 0, 3, 6, and 9 during cold storage at 4℃. The pH value of all treatments was lower than that of the control (P<0.05). Total microorganisms of CJ and LCJ at day 9 was 6.94 and 6.76 log CFU/g, respectively, whereas that of the control was 7.51 log CFU/g. The a* value of CJ and LCJ was higher than that of CON and LJ during storage (P<0.05), whereas the b* value of LJ, CL, and LCJ was lower than that of CON at day 6 and 9 (P<0.05). Overall acceptability of all treatments was significantly higher than that of CON after day 3. Thiobarbituric acid reactive substances and volatile basic nitrogen values were lower than those of the CON after day 3 (P<0.05). Principle component analysis (PCA) of the aroma pattern of all treatments was closer together, whereas PCA of the CON was scattered with the increase in storage days. This result suggests that dipping the chicken thigh meat into the lemon and cranberry juice could be beneficial to enhance chicken thigh meat quality by retardation of total microbes, lipid oxidation, and protein decomposition.

      • Molecular networks underlying ovarian aging for clinical applications

        YoungHo Roh,SookRyung Kim,EunJung Choi,YoungJoo Kim,HyangGi Park,PureunNarae Kang,DaJung Chung,NaYoung Kim,MinJae Kim,AeRa Han,KwangRae Kim,Chan Park,YongPil Cheon,YoungJin Lee 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8

        Due to modern trends with postponing child-bearing and getting worse living environment in women, an ovarian aging increased pregnancy failure and other complications with menopause or premature ovarian failure. Although several theories have been suggested such as mitochondrial malfunction, DNA damage/repair/methylation, caloric restriction, studies regarding ovarian aging-related molecular mechanisms for development of therapeutic methods are insufficient so far. Our objective is to determine molecular pathways of ovarian aging that result in pregnancy failure and other complications in women health to develop treatment strategies. This study is consisted of two parts: in Phase I stage, we analyzed distinct gene expression profile between young and aged mouse ovaries, and in Phase II stage several preferentially expressed genes in both ovaries were selected and analyzed their physiological functions and involved molecular networks related to ovarian aging for development of diagnostic markers and therapeutic methods. Ovaries from 10 week and 11 month-old FVB/NJ female mice with synchronized estrus cycle were collected for this study. A half of each ovary was used for RNA preparation and the other half for histological analysis. Using the Illumina HiSeq 2000 System, preferentially expressed genes were identified. Functional annotation database-based gene-set enrichment analyses and Pathway Studio® were employed to evaluate aging-related molecular networks. These findings were confirmed through qRT-PCR and immunohistochemistry. To validate RNA-Seq data, we examined expression patterns of marker genes (Amh, Bmp15 and Nobox) that were wellknown to be decreased in ovarian aging process. In young or aged ovary, preferentially expressed 876 genes were identified and extracellular matrix (ECM; p<0.001) and chromatin/nucleosome-related (p<0.001) protein-coded genes have the majority in these genes by GOTERM analysis. Amongthem, we selected several candidate genes and confirmed their expression profiles by qRT-PCR and immunohistochemistry followed by molecular network analysis. Regarding molecular interactions in these genes, PathwayStudio® was employed to predict aging-involved molecular networks in mouse ovary. Here we report a couple of candidate molecular networks and medicines (chemicals) for targeting these preferentially expressed genes/proteins. Further analyses are scheduled to produce transgenic animal models and with human ovarian tissues/cell lines.

      • KCI등재

        Effect of Dihydroxybenzoic Acid Isomers on the Analysis of Polyethylene Glycols in MALDI-MS

        Aera Lee,Hyo-Jik Yang,Yangsun Kim,Jeongkwon Kim 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.5

        The effects of different dihydroxybenzoic acid (DHB) isomers, when used as matrix materials in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), were investigated in analyses of polyethylene glycol(PEG) polymers. PEG polymers ranging from 400 to 8,000 Da were prepared in different DHB isomer matrices using solvent-based and solvent-free methods. PEG samples were detected only in matrices of 2,3-DHB, 2,5-DHB,and 2,6-DHB while the most intense peaks were observed using 2,6-DHB in both solvent-free and solvent-based preparations.

      • Prognostic factors and outcomes in endometrial stromal sarcoma with the 2009 FIGO staging system; A multicenter review of 114 cases

        ( Aera Yoon ),( Jeong Yeol Park ),( Jin Young Park ),( Yoo Young Lee ),( Tae Joong Kim ),( Chel Hun Choi ),( Duk Soo Bae ),( Byoung Gie Kim ),( Jeong Won Lee ),( Joo Hyun Nam ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

        To assess the prognostic factors associated with disease-related survival in endometrial stromal sarcoma (ESS), to which the 2009 FIGO staging system was applied. From January 1990 to January 2012, 114 patients with ESS were identified at the Samsung and Asan Medical Center and data were retrospectively analyzed. Ten (8.7%) patients died of the disease and 33 (28.9%) patients relapsed. The 5- and 10-year overall survival (OS) rates for the entire cohort was 92.6% and 87.1%, and the 5- and 10-year recurrence-free survival (RFS) rates were 71.8% and 52.1%. The estimated median survival after recurrence of 33 patients was 133 months (95% CI, 7.7 - 258.4), and 5-year survival after recurrence was 68.9%, retrospectively. Stage I (p=0.006), positive of estrogen and/or progesterone receptor (ER/PR) (p=0.0027) and no nodal metastasis (p=0.033) were associated with a good prognosis for OS in the univariate analysis. Ovarian preservation was revealed to be an independent predictor for poorer RFS (HR, 6.5; 95% CI, 1.23 - 34.19; p=0.027). Positivity of ER/PR (HR, 0.05; 95% CI, 0.006 - 0.4; p=0.006) and cytoreductive resection of recurred lesions (HR, 0.14; 95% CI, 0.02 - 0.93; p=0.042) were independent predictors for better survival after recurrence. Stage, expression of ER/PR and nodal metastasis are significantly associated with OS in ESS. Bilateral salpingo-oophorectomy (BSO) as the primary treatment and cytoreductive resection of recurred lesions should be considered for improving survival of patients with ESS.

      • Evaluation of New FIGO Staging System for Endometrial Stromal Sarcoma of the Uterus

        ( Aera Yoon ),( Jeong Yeol Park ),( Yoo Young Lee ),( Tae Joong Kim ),( Chel Hun Choi ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

        The aim of this study is to detect differences in overall survival between the 1988 FIGO staging and revised 2008 FIGO staging of uterine endometrial stromal sarcoma. The aim of this study is to detect differences in overall survival between the 1988 FIGO staging and revised 2008 FIGO staging of uterine endometrial stromal sarcoma. Of 109 patients with LGESS, 86 patients (78.9%) had stage I disease, 5 patients (4.6%) had stage II disease, 9 patients (8.3%) were diagnosed as stage III, and 9 patients (8.3%) had stage IV disease by 1988 FIGO staging system. According to revised 2008 FIGO staging system, 8 patients (7.3%) were downstaged; 3 patients were downstaged from stage II to I and 5 patients were downstaged from stage III to II respectively. The difference in median survival of 4 stages (I-IV) was statistically significant using both staging system (1998 vs. 2008 FIGO staging system, p=0.006 vs. p=0.013). Comparing the median survival of 2 subgroups, both staging system had significant difference but previous staging system had more accurate discriminability (1998 vs. 2008, p=0.006 vs. p=0.019, HR 5.470 vs.4.286). Based upon our analysis of 109 patients with uterine LGESS, the revised FIGO staging system was appropriate to predict survival.

      • SCIESCOPUSKCI등재

        Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates

        Kim, Dongwook,Kim, Hee-Jin,Chae, Hyun-Seok,Park, Nam-Gun,Kim, Young-Boong,Jang, Aera Korean Society for Food Science of Animal Resource 2014 한국축산식품학회지 Vol.34 No.6

        This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was $364.72{\mu}M/TE$ and ORAC activity (1 mg/mL) was $101.85{\mu}M/TE$. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of $100mJ/cm^2$. After 24 h of incubation with $500{\mu}g/mL$ LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.

      • SCIESCOPUSKCI등재

        Effects of Doneness on the Microbial, Nutritional, and Quality Properties of Pork Steak of Different Thicknesses

        Aera Jang,Hye-Jin Kim,Dongwook Kim,JinSoo Kim,Sung-Ki Lee 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.5

        This study aimed to evaluate the effect of doneness on the microbial, nutritional, and quality characteristics of 1.5 cm- and 2.0 cm-thick pork neck steaks. Pork neck meat was obtained within 24 h after slaughtering, cut into 1.5 cm- and 2.0 cm-thick slices (n=5), packed in LLD-PE wrap, and stored at 4±2℃ for 7–10 days until aerobic plate counts (APC) reach 5.51–6.50 Log CFU/g. Then, the pork meat was cooked on a frying pan till it was medium-rare, medium, or well-done. The microbial inhibition rates of the 1.5 cm- and 2.0 cm-thick steak in medium-rare state were 58.26% and 51.70%, respectively, whereas it was 100% for medium-done pork steak of either thickness. The total calories of the 1.5 cm- and 2.0 cm-thick well-done pork steaks were 643.61 kcal/100 g and 675.00 kcal/100 g, respectively, which was higher than that in medium-rare and medium-done steaks. The retention ratios for Fe and K in the well-done steak were significantly lower than those in the medium and medium-rare steak of either thickness (p<0.05). The shear force of the medium-rare and medium steak did not differ, whereas that of the well-done steak was significantly higher than that of the medium-rare steak of either thickness (p<0.05). We observed that the well-done pork steak had tough texture, low mineral content, and high calories. Therefore, consumption of medium and mediumrare pork is more beneficial than that of well-done pork.

      • SSCISCOPUSKCI등재

        Gender and labor ethics in aesthetic labor : Female students of specialized vocational home economics high schools in Korea

        Kim Aera Asian Center for Women's Studies : Ewha Womans Uni 2018 Asian Journal of Women's Studies(AJWS) Vol.24 No.3

        본 연구는 디지털미디어의 대중화와 신한류, 소비문화산업의 팽창과 함께 2000년 중반 이후 한국의 중고등 교육 분야에서 관련 전공이 증가하고 있으며, 여학생들 사이에서 쇼핑, 미용, 패션 등에서의 서비스노동인 심미노동 영역이 각광받고 있는 현상에 대한 질문에서 시작한다. 이와 같은 현상은 특히 2010년 디지털 미디어의 대중화와 ‘한류’의 확장 속에 있다. 본 연구는 주로 특성화고 여학생들이 패션, 뷰티 등 심미노동 영역으로 진입하게 되는 경우가 많다는 점에 주목, 특성화고등학교 여학생들이 심미노동에 진입하는 맥락을 살펴보고자 한다. 이를 위해 여학생들이 심미노동에 부여하는 문화적 의미와 교육제도와의 연관성을 탐색한다. 이 같은 작업은 여학생들의 심미노동에의 동기와 특성화고등학교의 성별화된 정책 사이 관계를 탐색하는 것이며 또한 오늘날 신자유주의 노동윤리와 성별의 문제들이 교차되는 지점을 분석하는 것이기도 하다. This study begins by asking why the aesthetic labor industry in Korea has been in the limelight, and related disciplines have been on the rise in secondary and higher education as of the mid-2000s. This has happened along with the popularization of digital media and the expansion of the ‘Korean wave’ as of the 2010s. This study focuses on the common practice for female students from specialized vocational high schools to work in aesthetic labor today, such as the cosmetics, fashion or beauty business. It seeks to examine the phenomenon of female students who enter these schools in order to work in the fashion and beauty industries and understand the relationship between the educational system and the cultural meanings they assign to aesthetic labor. It will also explore the correlation between their motivation to undertake such labor and the gendered policy of specialized vocational high school education. This work thereby examines the intersection of neo-liberal labor ethics and gender in Korean society.

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