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파노라마 촬영시의 두경부 주요기관의 등가선량, 유효선량 및 위험도
이애련,나경수,조봉혜 大韓口腔顎顔面 放射線學會 1995 Imaging Science in Dentistry Vol.25 No.2
The purpose of this study was to evaluate the equivalent and effective dose, and estimate radiation risk to the critical organs of head and neck region from the use of adult and child mode in panoramic radiography. The results were as follows. 1. The salivary glands showed the highest equivalent and effective does in adult mode were 837μSv and 20.93μSv, those in child mode were 462μSv and 11.54μSv, respectively. 2. Total effctive doses to the critical head and neck organs were estimated 34.21μSv in adult mode, 20.14μSv in child mode. From these datas, The probabilities of stochastic effect from adult and child mode were ?? and ??. 3. The other remainder showed the greatest risk of fatal cancer. The risk estimate were 4.5 and 2.7 fatal malignancies in adult and child mode from million panoramic examinations. The bone marrow and thyroid gland showed about 0.1 fatal cancer in adult and child mode from these examinations.
Expression of Raf Proto - Oncogene During Drosophila Oogenesis and Development
이원호,유미애,류재현 한국유전학회 1994 Genes & Genomics Vol.16 No.3
The RNA expression pattern for Draf, a Drosophila homolog of Raf-1, during Drosophila oogenesis and development were examined by in situ hybridization. During oogenesis, Draf transcripts are not present in germarium region but present in nurse-oocyte cell complex from stage 2 onwards. In mature oocyte, the maternally derived Draf transcripts are homogeneously distributed. At cellular blastoderm stage, Draf transcripts are predominantly distributed in the peripheral cells. During and after gastrulation, regions corresponding to cell proliferation, the neural cell precursors and the central nervous system express Draf at high levels. During larval development, Draf is strongly expressed in the imaginal disc cells and the salivary glands. The strong expression of the Draf in the central nervous system during embryogenesis suggest that the Draf gene may also play a role in the nervous system.
Studies on the function of DNA Polymerase β Using Transgenic Drosophila
이원호,유미애,류재련,하혜영 한국유전학회 1994 Genes & Genomics Vol.16 No.3
The biological function of DNA polymerase β in the whole organism was examined by using a transgenic Drosophila bearing chimeric gene consisting of a promoter region of Drosophila actin 5C gene and rat DNA polymerase β cDNA. Southern analysis of genomic DNA of transgenic flies and transmission pattern of p[w^+;polβ] indicated that a single copy of p[w^+;polβ] was integrated into each transgenic strain. To elucidate the role of DNA polymerase β in DNA repair, the sensitivity of transgenic strain to killing after treatment of MMC, MMS and UV were compared with that of host strain. The sensitivity of transgenic strain to mitotic recombination induced by MMC or MMS were also compared with host strain. The present results suggest that rat DNA polymerase β expressed in transgenic flies play an important role at least in the generation process of mitotic recombination.
Ryu, Jae-Ryeon,Choi, Thae-Yeong,Kwon, Eun-Jeong,Lee, Won-Ho,Nishida, Yasuyoshi,Hayashi, Yuko,Matsukage, Akio,Yamaguchi, Masamitsu,Yoo, Mi-Ae 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-
The DRE/DREF system plays an important role in transcription of DNA replication genes such as those encoding the 180 and 73 kDa subunits of DNA polymeraseαas well as that encoding PCNA. In this study, we found two sequences homologous to DRE(5'-TATCGATA-3')in the 5'-flanking region(-370 to-357 with respect to the transcription initiation site) of the D-raf gene and confirmed transcription activity through gel mobility shift assay, transient CAT assays, and spatial patterns of lacZ expression in transgenic larval tissues carrying D-raf gene is another target of the Zerknu¨llt(Zen)protein with observation of D-raf repression by Zen protein in cultured cells anf its ectopic expression in the dorsal region of the homozygous zen mutant embryo. The evdience of DRE/DREF involvement in regulation of the D-raf gene obtained in this study strongly supports the idea that the DRE/DREF system is responsible for the coordinated regulation of cell proliferation-related genes in Drosophila.
Ryu, Jae-Ryeon,Kim, Young-Shin,Kim, Hyeong-In,Lee, Heui-Jung,Lee, Won-Ho,Yoo, Mi-Ae 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-
D-raf, a Drosophila homolog of Raf - 1, functions as a signal transducer in cell proliferation and differentiation. The reporter plasmid p5'-1103 Draf-lacZ fused the Draf gene regulatory region (-1103~+300 with respect to the translation initiation site) with the lacZ gene in a P-element vector was constructed. Expression of the Draf-lacZ fusion gene was demonstrated in Kc cells by transient expression assay. Transgenic lines bearing Draf-lacZ fusion gene were established by P-element mediated transformation with the reporter plasmid. Spatial pattern of the Draf-lacZ expression in transgenic embryos and ovaries was similar to the distribution of the endogenous D-raf transcripts examined by in situ hybridization in previous study. We first report the expression of D-rsf gene in larval fat body analogous to the vertebrate liver and in testis and anterior ejaculatory duct of adult male. And our results show that the regulatory region is sufficient for expression of the D-raf gene. Draf-lacZ transgenic flies obtained in this study promise to be useful for studying multiple roles and regulation of expression of D-raf gene.
Yoo, Mi-Ae,LEE, Won-Ho,HA, Hye-Yeong,RYU, Jae-Ryeon,YAMAGUCHI, Masamitsu,FUJIKAWA, Kazuo,MATSUKAGE, Akio,KONDO, Sohei,NISHIDA, Yasuyoshi 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-
DNA polymerase β(pol β)cDNA of rat fused to an enhamcer-promoter ergion plus a poly(A) signal sequence of actin 5C gene of Drosophila(abbreviated polβ) was transferred to the Drosophila genome. Three of four constructed transgenic strains possessing transgene polβ on different chromosomes were studied. Levels of the polβ transcript and those of the polymerization activity of polβ were markedly elevated in cultured cells trans fected with polβ-bearing vectors as well as in embryos of the transgenic strains. The popular idea that DNA polymerase β participates in DNA repair was not supported by the observation that a pair of a normal and a polβ strain, and the other pair of a mei-9 mei-41(DNA-repair deficient double mutations) strain and a polβmei-9 mei-41 strain, showed no difference in survial within each pair after treatment with ultraviolet light, methylmethane sulfonate and mitomycin C. The other idea that DNA polymerase β participates in recombination was supported by the findings that spontaneous freauency of recombination, either meiotic or mitotic, is significantly higher in a transgenic polβstrain than in a non-transgenic strain. The enhanced recomdination frequency in the polβ strain may, however, reflect an indirect effect of over-produced polβ prteins on chromosomal stability. Whatever the direct effect of rat polβis, the transgenic polβ flies will be useful for study of the physiological role of polβ and the mechanism of recombination.