Characterization of JAK2 V617F (1849 G > T) Mutation in Cervical Cancer Related to Human Papillomavirus and Sexually Transmitted Infections

Masoumeh Abdolmaleki,Amir Sohrabi 대한암예방학회 2018 Journal of cancer prevention Vol.23 No.2

Background: Human papillomavirus (HPV) genotypes that infect the genital tract play a main etiologic role in cervical cancer progression. Other environmental factors, such as sexually transmitted diseases and the host genetic pattern, contribute to infection persistence of the uterus and cervical epithelium in sustaining their malignancy. The Janus kinase 2 is a non-receptor tyrosine kinase in cell signaling process of tumor genesis. In the present study, JAK2 V167F mutation was distinguished in women with sexually transmitted infections, such as Herpes simplex virus 2, Chlamydia trachomatis and Mycoplasma genitalium and cervical cancer. Methods: This case-control survey was performed on 195 liquid based cytology of women specimens. Fifty, 98, and 47 samples were from women with known cervical cancer, HPV positive and HPV negative, respectively. Single nucleotide polymorphism analysis, sexually transmitted infections detection and HPV genotyping were carried out using approved PCR- RFLP, in-house multiplex TaqMan Real Time PCR and the reverse dot blot hybridization assay. Results: HPVs 6, 16, 18, 11, 31, and 51 were the most common genotypes. The prevalence rate of multiple HPV genotypes was 46.0% to 10.1%. Analysis of JAK2 V617F (1849 G > T) showed that prevalence of mutation was GG (65.1%), GA (34.9%), and TT (0%), respectively. There were no statistically significant differences between this mutation and variables of population survey (P ≥ 0.05). Conclusions: The molecular epidemiology study on the genetic polymorphisms, i.e., JAK2 V617F and other single nucleotide polymorphisms as a diagnostic tool is necessary for cancer screening and prophylactic programs.

Concentrated O/W emulsions formulated by binary and ternary mixtures of sodium caseinate, xanthan and guar gums: rheological properties, microstructure, and stability

Khadije Abdolmaleki,Leyla Alizadeh,Seyede Marzieh Hosseini,Kooshan Nayebzadeh 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.12

The effects of xanthan gum (XG) (0, 0.3, 0.6wt%), guar gum (GG) (0, 0.3, 0.6 wt%) and XG:GGmixtures (0.3–0.3, 0.3–0.6, 0.6–0.3 and 0.6–0.6 wt%) onthe physical stability of sodium caseinate (CN) stabilizedconcentrated O/W emulsions (uoil = 0.6) were examined. The emulsion stability, microstructure, droplets size distribution,and rheological properties were evaluated. Thefindings showed that with increasing total gum concentrationup to 0.6% droplets size and emulsion instabilitysignificantly decreased (p\0.05). The emulsion containinga ternary mixture of CN:XG:GG at total gum concentration(0.6%) with a mixing ratio of 0.3:0.3 XG:GGexhibited the best stability with the highest ESI value(98.3%). Above the critical concentration, an excessiveincrease in storage modulus led to a significant increase indroplet size and emulsion instability. In brief, concentratedemulsions stabilized by binary and ternary mixtures (CN/XG/GG) may be applicable in special food like heavycream and as a template for fabricating oleogels.

A laboratory and numerical study on the effect of geogrid-box method on bearing capacity of rock-soil slopes

Moradi, Gholam,Abdolmaleki, Arvin,Soltani, Parham,Ahmadvand, Masoud Techno-Press 2018 Geomechanics & engineering Vol.14 No.4

Currently, layered geogrid method (LGM) is the commonly practiced technique for reinforcement of slopes. In this paper the geogrid-box method (GBM) is introduced as a new approach for reinforcement of rock-soil slopes. To achieve the objectives of this study, a laboratory setup was designed and the slopes without reinforcements and reinforced with LGM and GBM were tested under the loading of a circular footing. The effect of vertical spacing between geogrid layers and box thickness on normalized bearing capacity and failure mechanism of slopes was investigated. A series of 3D finite element analysis were also performed using ABAQUS software to supplement the results of the model tests. The results indicated that the load-settlement behavior and the ultimate bearing capacity of footing can be significantly improved by the inclusion of reinforcing geogrid in the soil. It was found that for the slopes reinforced with GBM, the displacement contours are widely distributed in the rock-soil mass underneath the footing in greater width and depth than that in the reinforced slope with LGM, which in turn results in higher bearing capacity. It was also established that by reducing the thickness of geogrid-boxes, the distribution and depth of displacement contours increases and a longer failure surface is developed, which suggests the enhanced bearing capacity of the slope. Based on the studied designs, the ultimate bearing capacity of the GBM-reinforced slope was found to be 11.16% higher than that of the slope reinforced with LGM. The results also indicated that, reinforcement of rock-soil slopes using GBM causes an improvement in the ultimate bearing capacity as high as 24.8 times more than that of the unreinforced slope.

ON A SPLITTING PRECONDITIONER FOR SADDLE POINT PROBLEMS

Davod Khojasteh Salkuyeh,MARYAM ABDOLMALEKI,SAEED KARIMI 한국전산응용수학회 2018 Journal of applied mathematics & informatics Vol.36 No.5

Cao et al. in (Numer. Linear. Algebra Appl. 18 (2011) 875-895) proposed a splitting method for saddle point problems which un- conditionally converges to the solution of the system. It was shown that a Krylov subspace method like GMRES in conjunction with the induced preconditioner is very eective for the saddle point problems. In this paper we rst modify the iterative method, discuss its convergence properties and apply the induced preconditioner to the problem. Numerical experiments of the corresponding preconditioner are compared to the primitive one to show the superiority of our method.

Heavy metal remediation via poly(3,4-ethylene dioxythiophene) deposition onto neat and sulfonated nonwoven poly(ether sulfone)

Zahra Mohamadi,Amir Abdolmaleki 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.55 No.-

Poly(ether sulfone) (PES) and sulfonated poly(ether sulfone) (SPES) nanofiber mats were synthesized by electrospinning and modified via the in-situ polymerization of poly(3,4-ethylene dioxythiophene) (PEDOT) on nonwoven (NW) electrospun PES and SPES mats. Chelating property of the obtained nanofiber mats were evaluated (for Pb(II), Cd(II) and Cr(VI) ions). The adsorption capacity was influenced by pH, concentration and contact time and followed a pseudo-second-order kinetics model (Freundlich model). The adsorption capacities of NW PEDOT/SPES mats were remarkably greater than NW PEDOT/ PES mats. The charge transport characteristics electrical impedance (EIS) results indicated that they were sensitive to the nature of adsorbed metal ions.

ON A SPLITTING PRECONDITIONER FOR SADDLE POINT PROBLEMS

SALKUYEH, DAVOD KHOJASTEH,ABDOLMALEKI, MARYAM,KARIMI, SAEED The Korean Society for Computational and Applied M 2018 Journal of applied mathematics & informatics Vol.36 No.5

Cao et al. in (Numer. Linear. Algebra Appl. 18 (2011) 875-895) proposed a splitting method for saddle point problems which unconditionally converges to the solution of the system. It was shown that a Krylov subspace method like GMRES in conjunction with the induced preconditioner is very effective for the saddle point problems. In this paper we first modify the iterative method, discuss its convergence properties and apply the induced preconditioner to the problem. Numerical experiments of the corresponding preconditioner are compared to the primitive one to show the superiority of our method.

Properties of Solanum melongena green calyx against toxic effects of diabetes‐induced testopathy: a stereological and biochemical study

Ahmad Shabanizadeh,Shiva Roshankhah,Amir Abdolmaleki,Mohammad Reza Salahshoor 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.3

Solanum melongena green calyx (SMGC) with antioxidant features seems to have many therapeutic features. Diabetes mellitus (DM) is a blood sugar disorder that increases oxidative stress levels in various types of cells leading to testopathy. In this study we aimed to investigate the protective functions of hydro-alcoholic extract of SMGC on DM‐induced testicular damages. Mature male rats were randomly divided into five groups (n = 8); 1: control, 2: diabetic, 3: diabetic + 100 mg/kg of SMGC, 4: diabetic + 300 mg/kg of SMGC, and 5: diabetic + 500 mg/kg of SMGC. Intraperitoneal injection of streptozotocin (STZ, a single dose of 60 mg/kg) was used for DM induction. Testicular alterations were evaluated quantitatively based on stereological techniques and followed by antioxidant enzymes determination (catalase, superoxide dismutase, and glutathione peroxidase), and serum testosterone level. Malondialdehyde and Bcl‐2 protein expression were also assessed biochemically. DM caused significant harmful changes in testicular structure, inhibition of antioxidant enzymes activity, decreased testosterone levels, and increased lipid peroxidation (LP). The expression of Bcl‐2 protein was down-regulated in DM testis along with boosted apoptosis rate. Eight weeks of SMGC treatment (particularly in high doses) recovered structural variations significantly, restored the power of the antioxidant defense system, and modified the testosterone levels. These findings revealed that the SMGC is considered as an effective antioxidant agent to decrease the adverse effects of DM on male reproductive system.

Synthesis and Characterization of New Dental Composite Containing Xanthene Based on Fish Bone Powder: Study on Anticancer and Antimicrobial Activity

S. Mojtaba Amininasab,Elham Ghoseiri,Sara Abdolmaleki 한국고분자학회 2022 Macromolecular Research Vol.30 No.12

In this study, we designed and synthesized a new dental composite including Spiro [fluorene-9,9’-xanthene]-3',6’-diylbis (oxy) bis (2-hydroxypropane-3,1-diyl) bis (2-methylacrylate) (Xanthene-GMA) resin containing xanthene and fluorene parts and natural filler of fish bone powder with low water sorption and solubility and excellent biocompatibility. This dental composite reduces the risks that Bisphenol A derivatives released from composite Bis-GMA may pose to the oral environment. The Xanthene-GMA resin was synthesized from the condensation reaction in the presence of an acid catalyst. The surface of the fish bone powder was also modified by (3-trimethoxysilyl) propyl methacrylate (Silane A174) modifier for creating better bonding with the resin. The structural characterization of the compounds was confirmed by Fourier transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (NMR), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), and thermogravimetric analysis (TGA) techniques. Then, the dental composite (Xanthene-GMA resin based on modified fish bones powder (XGMFB)) was prepared by heat and light curing. Finally, water sorption, solubility, cytotoxicity and antibacterial activity tests were evaluated. The thermal resistance of the composite was low due to the use of natural filler in fish bone powder. The composite showed low water sorption and solubility. The antibacterial and cytotoxicity activity of the composite was evaluated. S. aureus (MIC 16 μg/mL) and E. coli (MIC 32 μg /mL) were recognized as most susceptible gram-positive and gram-negative bacteria, respectively, after XGMFB treatment. The most inhibitory effect of XGMFB was observed against MCF7 cells (IC50 = 73.5μM, Maximum Inhibitory effect 78.18%).

Steel dual-ring dampers: Micro-finite element modelling and validation of cyclic behavior

Mahdi Usefvand,Ali Mohammad Rousta,Mojtaba Gorji Azandariani,Hamid Abdolmaleki 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.4

Extensive studies have been performed by researchers to increase the ductility and energy-absorption of concentrically braced frames. One of the most widely used strategies for increasing ductility and energy-absorbing is the utilization of energy-dissipation systems. In this regard, the energy-dissipation system consisting of a steel dual-ring damper (SDRD) with different construction details is presented, to improve hysteresis behavior and performance of steel ring dampers (SRD). The most important cause of energy-dissipation in SRDs are the development of bending plastic hinges in the rings. Therefore, by adding an inner ring to the SDR system, it increases the number of moment plastic hinges and in turn increases energy dissipation. Parametric studies havse been performed applying the nonlinear micro-finite element (MFE) procedure to investigate the improved models. The parametric studies comprise examining the efficacy of thickness parameters and the inner ring diameters of the improved models. The SRD models was selected as the base model for comparing and evaluating the effects of improved dampers. MFE models were then analyzed under cyclic loading and nonlinear static methods. Confirmation of the results of the MFE models were performed against the test results. The results indicated that the diameter to the thickness ratio of inner ring of SDRDs has a considerable influence on determining the hysteresis behavior, ductility, ultimate capacity and performance, as well as energy dissipation. Also, the results show that the details of the construction of the internal and external ring connections were a considerable effect on the performance and hysteresis behavior of SDRDs.

Proposing a dynamic stiffness method for the free vibration of bi-directional functionally-graded Timoshenko nanobeams

Mohammad Gholami,Mojtaba Gorji Azandariani,Ahmed Najat Ahmed,Hamid Abdolmaleki Techno-Press 2023 Advances in nano research Vol.14 No.2

This paper studies the free vibration behavior of bi-dimensional functionally graded (BFG) nanobeams subjected to arbitrary boundary conditions. According to Eringen's nonlocal theory and Hamilton's principle, the underlying equations of motion have been obtained for BFG nanobeams. Moreover, the variable substitution method is utilized to establish the structure's state-space differential equations, followed by forming the dynamic stiffness matrix based on state-space differential equations. In order to compute the natural frequencies, the current study utilizes the Wittrick-Williams algorithm as a solution technique. Moreover, the nonlinear vibration frequencies calculated by employing the proposed method are compared to the frequencies obtained in previous studies to evaluate the proposed method's performance. Some illustrative numerical examples are also given in order to study the impacts of the nonlocal parameters, material property gradient indices, nanobeam length, and boundary conditions on the BFG nanobeam's frequency. It is found that reducing the nonlocal parameter will usually result in increased vibration frequencies.