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Mahmoud, Ahmed H.,Mashaly, Ashraf M.,Rady, Ahmed M.,Al-Anazi, Khalid M.,Saleh, Amgad A. Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2
Objective: This study was designed to characterize the DNA polymorphisms of the melanocortin-1 receptor (MC1R) gene in indigenous Saudi Arabian sheep breeds exhibiting different color coats, along with individuals of the Sawaknee breed, an exotic sheep imported from Sudan. Methods: The complete coding region of MC1R gene including parts of 3' and 5' untranslated regions was amplified and sequenced from three the indigenous Saudi sheep; Najdi (generally black, n = 41), Naeimi (generally white with brown faces, n = 36) and Herri (generally white, n = 18), in addition to 13 Sawaknee sheep. Results: Five single nucleotide polymorphisms (SNPs) were detected in the MC1R gene: two led to nonsynonymous mutations (c.218 T>A, p.73 Met>Lys and c.361 G>A, p.121 Asp>Asn) and three led to synonymous mutations (c.429 C>T, p.143 Tyr>Tyr; c.600 T>G, p.200 Leu>Leu, and c.735 C>T, p.245 Ile>Ile). Based on these five SNPs, eight haplotypes representing MC1R $E^d$ and $E^+$ alleles were identified among the studied sheep breeds. The most common haplotype (H3) of the dominant $E^d$ allele was associated with either black or brown coat color in Najdi and Sawaknee sheep, respectively. Two other haplotypes (H6 and H7) of $E^d$ allele, with only the nonsynonymous mutation A218T, were detected for the first time in Saudi indigenous sheep. Conclusion: In addition to investigating the MC1R allelic variation in Saudi indigenous sheep populations, the present study supports the assumption that the two independent nonsynonymous Met73Lys and Asp121Asn mutations in MC1R gene are associated with black or red coat colors in sheep breeds.
Strain-Induced Spin States in Atomically Ordered Cobaltites
Choi, Woo Seok,Kwon, Ji-Hwan,Jeen, Hyoungjeen,Hamann-Borrero, Jorge E.,Radi, Abdullah,Macke, Sebastian,Sutarto, Ronny,He, Feizhou,Sawatzky, George A.,Hinkov, Vladimir,Kim, Miyoung,Lee, Ho Nyung American Chemical Society 2012 NANO LETTERS Vol.12 No.9
<P>Epitaxial strain imposed in complex oxide thin films by heteroepitaxy is recognized as a powerful tool for identifying new properties and exploring the vast potential of materials performance. A particular example is LaCoO<SUB>3</SUB>, a zero spin, nonmagnetic material in the bulk, whose strong ferromagnetism in a thin film remains enigmatic despite a decade of intense research. Here, we use scanning transmission electron microscopy complemented by X-ray and optical spectroscopy to study LaCoO<SUB>3</SUB> epitaxial thin films under different strain states. We observed an unconventional strain relaxation behavior resulting in stripe-like, lattice modulated patterns, which did not involve uncontrolled misfit dislocations or other defects. The modulation entails the formation of ferromagnetically ordered sheets comprising intermediate or high spin Co<SUP>3+</SUP>, thus offering an unambiguous description for the exotic magnetism found in epitaxially strained LaCoO<SUB>3</SUB> films. This observation provides a novel route to tailoring the electronic and magnetic properties of functional oxide heterostructures.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2012/nalefd.2012.12.issue-9/nl302562f/production/images/medium/nl-2012-02562f_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl302562f'>ACS Electronic Supporting Info</A></P>
Effect of Stress Aging Induced Precipitates on Corrosion Behavior of NiTi Shape Memory Alloys
A. Radi,J. Khalil‑Allafi,A. Heidarzadeh,G. G. Yapici,M. R. Etminanfar,S. Z. Mozafari,B. Rezaei‑Moghadam 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.10
In this study, the influences of the stress aging process on the electrochemical behaviors toward evaluating corrosion resistanceof NiTi shape memory alloys in the in-vitro condition have been investigated. For this approach, the samples havebeen manufactured by introducing multiple precipitation morphology in the alloy structure via applying different levels ofstresses during the aging process. The samples were characterized using multiply electron microscopy, electrochemicalmethods, X-ray diffraction, and differential scanning calorimetry. Results show that by prolonging aging time from 1 to 5 hand increasing the stress aging level (15–60-150 MPa) the corrosion resistance improves, which is implied a better formationof a protective layer. It seems that homogeneous precipitation of Ni-rich phases under the stress aging process improvesthe corrosion resistance of the alloy.
Assessment of Some Barley Germplasms Based on RAPD Analysis and Anti-nutritional Factors
Amal A. Mohamed,Mohamed Ahmed Matter,Ramadan Rady 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.2
The variation among some barley germplasm (Giza 123, Giza 127, Giza 130, Desert, and Sinai) was investigated comparatively at the molecular and biochemical levels. Leaf DNAs extracted from different barley germplasm were amplified with randomly chosen primers in a Polymerase Chain Reaction (PCR) using Random Amplified Polymorphic DNA (RAPD) technique. Only five primers amplified all DNA templates among 15 primers. Primers generated a total of 112 DNA fragments ranged from 1.2 to 0.1 kbp. Out of the 112 fragments, 16 (14.29%) were polymorphic. The result indicated that fragments generated per primer, with an average of 22.4products, where the number of polymorphic bands per primer, with an average of 3.2 polymorphic bands. In addition, the genetic variation in the anti-nutritional factors and nutrient composition of the same genotypes were determined. A wide variation in total phenolic, phytic, tannin, vicine, and saponin contents was found among these germplasm. The range of total phenolic was 3.83 to 7.50 mg g-¹ d.w. Among all germplasm, Desert germplasm was characterized by the highest tannin content. However, saponin ranged between 5.66 mg g-¹ d.w. (in Giza 123) to 9.21 mg g-¹ d.w (in Sinai) germplasm. The concentrations of the phytic acid were generally low, ranging between 0.99 to 6.78 mg g-¹ d.w. The germplasm Desert and Sinai had the highest level of vicine, 1.89 and 1.87 mg g-¹d.w., respectively. The results of the molecular characterization and anti-nutritional content can be used as the starting point needed to identify the valuable Egyptian barley germplasm.
Assessment of Some Barley Germplasms Based on RAPD Analysis and Anti-Nutritional Factors
Mohamed, Amal A.,Matter, Mohamed Ahmed,Rady, Mohamed Ramadan 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.2
The variation among some barley germplasm (Giza 123, Giza 127, Giza 130, Desert, and Sinai) was investigated comparatively at the molecular and biochemical levels. Leaf DNAs extracted from different barley germplasm were amplified with randomly chosen primers in a Polymerase Chain Reaction (PCR) using Random Amplified Polymorphic DNA (RAPD) technique. Only five primers amplified all DNA templates among 15 primers. Primers generated a total of 112 DNA fragments ranged from 1.2 to 0.1 kbp. Out of the 112 fragments, 16 (14.29%) were polymorphic. The result indicated that fragments generated per primer, with an average of 22.4 products, where the number of polymorphic bands per primer, with an average of 3.2 polymorphic bands. In addition, the genetic variation in the anti-nutritional factors and nutrient composition of the same genotypes were determined. A wide variation in total phenolic, phytic, tannin, vicine, and saponin contents was found among these germplasm. The range of total phenolic was 3.83 to $7.50\;mg\;g^{-1}d.w$. Among all germplasm, Desert germplasm was characterized by the highest tannin content. However, saponin ranged between $5.66\;mg\;g^{-1}d.w$. (in Giza 123) to $9.21\;mg\;g^{-1}d.w$ (in Sinai) germplasm. The concentrations of the phytic acid were generally low, ranging between 0.99 to $6.78\;mg\;g^{-1}d.w$. The germplasm Desert and Sinai had the highest level of vicine, 1.89 and $1.87\;mg\;g^{-1}d.w$., respectively. The results of the molecular characterization and anti-nutritional content can be used as the starting point needed to identify the valuable Egyptian barley germplasm.
( Chan Hyung Lee ),( Seong-joon Koh ),( Zaher A Radi ),( Aida Habtezion ) 대한장연구학회 2023 Intestinal Research Vol.21 No.3
Inflammatory bowel disease (IBD), comprising Crohn’s disease and ulcerative colitis, is a lifelong disease that manifests with chronic intestinal inflammation, sequential fibrosis, and an increased risk of colitis-associated colon cancer (CAC). The combined effects of genetic, immunological, environmental, and microbial factors render it difficult to determine the specific mechanism underlying the induction and perpetuation of IBD. Various animal models of IBD have contributed enormously to the understanding of IBD pathogenesis in terms of genomics, transcriptomics, proteomics, microbiome, and drug development of novel therapeutics. Although comprehensive research on IBD has been enabled by advanced technologies, such as genetically engineered models, there is a great need to develop relevant in vivo models of colitis and fibrosis. Here, we review 4 categories of animal models of acute and chronic intestinal inflammation, fibrosis, and CAC: chemically induced, genetically engineered, T cell transfer, and spontaneous gene mutation models. (Intest Res 2023;21:295-305)
Response of Wheat Growth and Productivity to Exogenous Polyamines under Lead Stress
Mostafa M Rady,Mohamed A Seif El-Yazal,Hanan AA Taie,Safia MA Ahmed 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.5
Polyamines [i.e. spermine (Spm), spermidine (Spd) and putrescine (Put)] antagonistic effects on stress imposed by 2.0 mM lead (Pb 2+ ) on growth, yield and its components and changes in the osmoprotectant and endogenous Pb 2+ concentrations, the contents of some nutrients and tissue health in wheat plants were evaluated. Under the three applied polyamine (PAs) applications, the efficiency of wheat plants to tolerate Pb 2+ stress in terms of growth and yield characteristics was noticed to varying degrees. The enhancements in osmoprotectant concentrations and plant health [in terms of relative water content (RWC) and membrane stability index (MSI)], and reductions in electrolyte leakage (EL) and plant Pb 2+ concentration were correlated with the reasonable growth of Pb 2+ -stressed plants and their grain yield. Results point out that, better growth and yield characteristics, MSI, RWC, leaf photosynthetic pigment and osmoprotectant concentrations, and nutrient contents were obtained with seed soaking in 0.25 mM Spm, 0.50 mM Spd or 1.0 mM Put than those generated with seed soaking in water under 2.0 mM Pb 2+ stress. In contrast, EL and the concentration of endogenous Pb 2+ were significantly reduced. However, the Pb 2+ -free control positively exceeded the all stressed treatments. Among all tested PAs, 1.0 mM Put showed the best results and thus is recommended, as seed soaking, for wheat to grow well under Pb 2+ stress.