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Physiological Characterization of an n-Butane-Utilizing Bacterium, Alcaligenes sp.
황태식,박두현,나병권 한국환경분석학회 2007 환경분석과 독성보건 Vol.10 No.3
An n-butane-utilizing bacterium was isolated from soil contaminated with petroleum and identified as Alcaligenes sp. by 16S-rDNA sequence homology. The bacterial isolate grew on gaseous alkane (n-butane), liquid alkane (n-hexane, n-octane or n-decane) or liquid alkane mixture (kerosene, C10~C16) as the sole carbon source, but did not grow on glucose, lactate or pyruvate. The specific activities of butane monooxygenase, butanol dehydrogenase, butyraldehyde dehydrogenase, lactate dehydrogenase, pyruvate dehydrogenase, isocitrate dehydrogenase and malate dehydrogenase were 6.97±0.42, 2.54±0.28, 3.78±0.31, 0.003±0.00, 0.007±0.00, 0.51±0.03 and 0.65±0.04 μM mg protein-1 min-1, respectively. In tests of n-butane, n-hexane, n-octane and ndecane oxygenation with crude enzyme extracted from bacterial cells grown on kerosene, NADH was oxidized to NAD+ coupled to production of n-butanol, n-hexanol, n-octanol and n-decanol. However, monooxygenase activity was relatively higher when the substrate for the enzyme assay was same as that used for growth than when the substrate and growth medium differed. This suggests that Alcaligenes sp. may produce specific monooxygenases dependent on the n-alkane species used as the growth substrate.
황태식,나병관,Hung Thuan Tran,안대희,박두현 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.6
In this study, a novel three-compartmented electrochemical bioreactor (3-CEB) was designed in an effort to overcome the disadvantages of the two-compartmented electrochemical bioreactor (2-CEB) separated with a cation-selective membrane for enrichment of strict anaerobes. The 3-CEB was comprised of an anode, outlet, and a cathode compartment. The outlet compartment was positioned between the anode and cathode compartment, and it was separated with the anode side by a rubber plate and with the cathode side by a porous glass membrane. A platinum wire bridging the anode and outlet compartment operated as a redox passage, however, through which no material could permeate. Butyrate fermentation bacteria were enriched on the basis of the metabolite production. Butyrate generated by strict anaerobes was significantly more abundant in the 3-CEB than in the 2-CEB. Acetic acid and lactic acid generated by facultative anaerobes was relatively higher in the 2-CEB than in the 3-CEB. Meanwhile, butyrate was not generated in the bioreactor utilized for the control test, to which the electrochemical potential was not charged. In a continuous culture using the 3-CEB, the majority of the glucose was fermented to butyrate, and the acetate additionally supplied to the bacterial culture was metabolically reduced to butyrate. More lactate than butyrate was generated from glucose in the 2-CEB.