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법랑질 표면의 탈회 및 재광화 후 EPMA (electron probe micro-analysis)를 이용한 칼슘, 인, 불소 변화의 정량적 분석
홍경식,허복,이찬영,금기연,Hong, Kyoung-Sik,Hur, Bock,Lee, Chan-Young,Keum, Ki-Yeon 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5
The aim of this in vitro study was to analyze the composition of human tooth enamel in terms of three components, Ca, P, and F after demineralization and remineralization in acid buffer solution. A total of 8 human premolars without any defects and cracks were selected and buccal and lingual sides of the teeth were cleaned with an ultrasonic device and pumice without fluoride 5$\times$5mm windows were opened, and other areas were completely covered with 3-coats of nail varnish to prevent from being in contact with demineralized and remineralized solutions. After demineralization process, each tooth was sectioned into two slices, highly polished one of them with$\gamma$-alumina, and then analyzed the composition of the demineralized tooth with EPMA(electron probe micro-analyzer). The other slices were put into the remineralized solution for 10 days, polished, and analyzed in the same manner. These data were statistically analyzed with one sample t-test(p<0.05). The results were as follows. 1. Normal tooth enamel consists of 49.76% Ca, 39.80% P, and 0.28% F. 2. After demineralization, percentage of Ca and P ratio were decreased by about 5.57 and 5.07% respectively. Percentage of F ratio was also decreased by about 0.01%, which was not statistically significant. 3. After remineralization, percentage of Ca, P increased about by 4.47 and 4.35% respectively Percentage of F decreased by about 0.01%, which was not statistically significant. In conclusion, remineralized solution used in our study has the potential to induce the uptake the Ca and P into the pore sites of the demineralized enamel. But, in the oral cavity. there were rapid temperature change, organic matrix that inhibits the movement of the ions, and limitation of continuous contact with this remineralized solution. Therefore, further in vivo study is necessary.
노의정 ( Eui Jung Roh ),홍경식 ( Kyoung Sik Hong ),정은희 ( Eun Hee Chung ),장영표 ( Young Pyo Chang ),이영석 ( Young Seok Lee ) 대한소아알레르기호흡기학회 2009 소아알레르기 및 호흡기학회지 Vol.19 No.3
Congenital syphilis is a completely preventable disease, but it still occurs. It usually involves multiple organs, which may cause neurological or skeletal disabilities or death in the fetus or newborn. Although lung involvement is rare in congenital syphilis, we should consider pneumonia alba when respiratory symptoms or radiologic findings are abnormal. We first report herein two cases of pneumonia alba with high resolution computerized tomography (HRCT) findings. [Pediatr Allergy Respir Dis(Korea) 2009;19:300-306]
법량질 표면의 탈회 및 재광화 후 EPMA (electron probe micro-analysis)를 이용한 칼슘, 인, 불소 변화의 정량적 분석
홍경식,허복,이찬영,금기연 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5
The aim of this in vitro study was to analyze the composition of human tooth enamel in terms of there components, Ca, P, and F after demineralization and remineralization in acid buffer solution. A total of 8 human premolars without any defects and cracks were selected and buccal and lingual sides of the teeth were cleaned with an ultrasonic device and pumice without fluoride. 5×5mm windows were opened, and other areas were completely covered with 3-coats of nail varnish to prevent from being in contact with demineralized and remineralized solutions. After demineralization process, each tooth was sectioned into two slices, highly polished one of them withγ-alumina, and then analyzed the composition of the demineralized tooth with EPMA(electron probe micro-analyzer) . The ocher slices were put into the remineralized solution for 10 days, polished, and analyzed in the same manner. These data were statistically analyzed with one sample t-test(p<0.05) . The results were as follows. 1. Normal tooth enamel consists of 49.76% Ca, 39.80% P, and 0.28% F. 2. After demineralization, percentage of Ca and P ratio were decreased by about 5.57 and 5.07% respectively. Percentage of F ratio was also decreased by about 0.01%, which was not statistically significant. 3. After remineralization, percentage of Ca, P increased about by 4.47 and 4.35% respectively. Percentage of F decreased by about 0.01%, which was not statistically significant. In conclusion, remineralized solution used in our study has the potential to induce the uptake the Ca and P into the pore sites of the demineralized enamel. But, in the oral cavity, there were rapid temperature change, organic matrix that inhibits the movement of the ions, and limitation of continuous contact with this remineralized solution. Therefore, further in vivo study is necessary.