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사염화탄소를 투여한 흰쥐의 간세포에 대한 식물 추출물들의 보호효과
함영국(Young-Kook Ham),김성완(Sung-Wan Kim) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.8
본 실험은 사염화탄소 독성에 대한 몇 가지의 식물소재들 - 솔잎, 콩나물, 인진쑥, 표고버섯 - 의 간보호 효과를 조사하기 위하여 수행되었다. 우선적으로 일차 간세포 배양실험에서 식물추출물(열수 또는 에탄올)을 첨가(20 ㎎/mL 배지)하고 2시간 후에 사염화탄소를 처리(50 μL)한 결과, 배지내의 AST와 ALT 효소활성은 다음과 같았다. 솔잎은 에탄올 및 열수 추출물(p<0.05)에서, 표고버섯은 열수 추출물(p<0.01 ~0.05)에서, 그리고 콩나물과 인진쑥은 추출방법에 따라 유의성 있는(p<0.01) 감소를 보였다. 또한, 3주간의 식물 추출물을 첨가한 사료를 사전급여하고 사염화탄소를 2일간 복강 주사(1 ㎎/kg 체중)한 동물실험에서 혈청내의 AST 및 ALT 활성은 간세포 배양의 결과와 유사하게 솔잎과 콩나물에서 낮은 활성효과(p<0.01)를 보였다. 정상군에 대한 사염화탄소의 투여는 지질과산화물과 cytochrome P-450의 함량, 그리고 xanthine oxidase(XOD) 활성을 크게 증가시켰다. 그러나 식물소재 중에서 솔잎, 콩나물, 인진쑥 등을 추출물을 첨가한 실험군에 대한 사염화탄소 처리는 일반적으로 지질과 산화와 XOD 활성증가를 억제하는 것(p<0.01)으로 나타난 반면에, cytochrome P-450의 함량 증가는 솔잎과 인진쑥의 첨가군에서 관찰되었다. 따라서 본 실험에서 cytochrome P-450의 함량증가에 대한 식물 추출물들의 효과는 확실하지 않았지만, 솔잎과 콩나물의 추출물은 항산화관련 실험에서 대부분 탁월한 효과를 나타내었다. To investigate the effects of plant extracts on the protection against liver damage by CCl₄ in rat, two kinds of experiment were performed, firstly by the primary hepatocyte culture and secondly by the animal feeding. The primary hepatocyte culture with the extracts of pine leaf, soybean sprout and mugwort showed significantly low activities (p<0.01~0.05) of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), indicating an excellent protective effect against liver damage by CCl₄. In the second experiment, the microsomal malondialdehyde (MDA) contents of the above same groups were also significantly lower (p<0.01) than the CCl₄-treated group without plant extracts, but shiitake showed less effect. Among four kinds of plant extracts, extracts of pine leaf and mugwort showed also much higher activities of the microsomal cytochrome P-450 in comparison to soybean sprout and shiitake. In the test of xanthine oxidase (XOD) activity, all of three groups except shiitake showed significantly low activities (p<0.01). These consistent results in vitro and in vivo suggest that the extracts of pine leaf, soybean sprout and mugwort may have strong protective effects against liver damage induced by the potential toxicants such as CCl₄.
DMBA 와 TPA 에 의해 유발되는 암 발생과정에서의 PKC 활성과 몇 가지 항산화 지표에 대한 비타민 A 와 E 의 영향
함영국,최면,김성완 ( Young Kook Ham,Myeon Choe,Sung Wan Kim ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4
Recombinant human interferon-β was purified to homogeneity from E. coli by methods of sonication, extraction with 8 M Guanidine HCl, solubilization of inclusion body, dilution, Blue Sepharose CL-6B column chromatography and HPLC gel filtration. Specific activity of purified IFN-β was 3.1×10^8 IU/㎎ protein and the purification was 1,902 fold. The purified IFN-β was a single band on SDS polyacrylamide gel electrophoresis under reducing condition and non-reducing condition and its molecular weight was estimated to 18,000 dalton. The results of N-terminal analysis showed that recombinant human IFN-β has N-terminal methionine same as natural human IFN-β. The inclusion bodies were observed in the E. coli cells harboring IFN-β gene but not observed in the host cells (MM 294). The purity of finally purified IFN-β was more than 99% by HPLC gel filtration.
저농도의 Microcystin-LR를 단기간 처리한 흰쥐에서 투여방법에 따른 독성 지표의 변화
함영국,김성완 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.3
To investigate the effects of low dosed microcystin-LR(MCLR) on the initial stage of subacute hepatotoxicity in vivo, male Sprague-Dawley rats after weanling were divided in four groups. The orally treated group(OG) was administered orally by 1㎍/kg B.W. at an interval of three days. The free group(FG) has taken the drinking water including 1㎍/L freely and the control group (CG) was only treated with 0.9% saline solution. All groups were treated for a period of 3 weeks. There was a significant correlation in body growth rate between OG and FG and especially, a deterioration of the growth of spleen was observed in the FG after 5 days. The protein levels were also decreased in OG and FG after 9 days. Level of total fat was increased to the 9th day but again decreased up to the initial level. High hemolysis of the isoiated erythrocytes occurred only in OG. Activities of γ-GT of OG and FG were higher twice-fold than CG, but the values of OG were already higher at the first treatment day. No significant change in aspartate aminotransferase(AST) activity was shown in all groups, but the activity of alanine aminotransferase(ALT) was slightly increased at the beginning state. There were much similarities in the results of OG and FG, except the growth inhibition of spleen in FG. It may be concluded that long-term effects of the low doses of mycrocystins in animals including human being can lead to serious health problems, especially to liver and spleen.