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최영국,김광제,이수복 ( Young Kook Choi,Kwang Je Kim,Soo Bok Lee ) 한국화학공학회 1989 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.27 No.5
A PVA pervaporation membrane, which has preferential selectivity for water, was introduced. For the separation of ethanol-water mixtures above 90 wt% of ethanol, the membrane exhibited selectivity above 50 and permeability of 0-0.4 ℓ/hr·㎡, The membrane surface area required for the production of 360 ㎏/hr absolute ethanol from 93 wt% ethanol-water mixture by the pervaporation process using the membrane was estimated as 163 ㎡. In this case, the average ethanol concentration was calculated as 13.5 wt%. The energy cost of pervaporation process was compared with that of azeotropic distillation process using benzene as an entrainer. As a result, it is confirmed that the energy cost of pervaporation process is merely 115 of that of azeotropic distillation process.
일체식 무교대 슬래브교의 구조거동 합리성과 내하력 성능평가 연구
최영국(Choi Young guk),장일영(Jang Il young) 한국방재학회 2017 한국방재학회논문집 Vol.17 No.6
기존의 거더형식의 교량이나 슬래브 교량에서는 신축이음부의 빈번한 파손으로 인한 유지관리 비용증가, 모멘트 재분배가 불리한 구조로 인한 내구성 저하 등의 문제가 있다. 이러한 문제점을 극복하기 위해 상부 바닥판과 접속슬래브가 일체식이면서 상부구조와 격벽거더 및 교각을 강결한 무조인트 무교대의 고차부정정 일체식 구조계를 제안하고 구조적 성능을 유한요소해석과 재하실험을 통해 비교 검증하였다. 일체식 무교대 슬래브구조는 기존공법의 교량에 비해 단면강성은 작지만 접속슬래브와 본체 구조가 연속 강결된 다연속· 프레임 구조계 형성과 상부구조의 경간과 단면강성의 균형으로 인하여 모멘트재분배와 힘전달이 확실하게 이루어져 기존교량에 비해 구조안전성, 연성 및 여용성이 높은 구조임을 확인하였다. In existing girder bridges and slab bridges, there are problems such as maintenance cost increase due to frequent breakage of expansion joints and durability reduction due to low moment redistribution structure. In order to overcome this problem, propose integral abutment bridge which is composed of the upper deck and the connecting slab integrally, and the finite element analysis and loading test results were compared. In experiment result, integral abutment bridge was the section stiffness is small but it is confirmed that the structural safety, ductility and flexibility are higher than existing bridges because the moment redistribution and the force transmission are surely performed.
Young-Hwa Cho(조영화),Yejin Choi(최예진),Jung-Hee Yun(윤정희),Nam Hee Kim(김남희),Mira Choi(최미라),Young-Kook Choi(최영국),Kyung-Hee Kim(김경희),Young-Ill Lee(이영일),Beom Jun Lee(이범준),Keerang Park(박기랑) 한국생명과학회 2012 생명과학회지 Vol.22 No.6
AAV는 매우 안전하고 효율적인 유전자전달방법으로 인정되어 왔다. 그러나, AAV가 가진 생물의약품으로서 단점은 효율적이고 재현성 높은 생산법이 취약하고, 또한 다양한 혈청형을 간단한 한 가지 공통적 방법으로 신뢰성 있게 정량하는 방법이 개발되어야 하는 것이다. 따라서, 본 연구에서는 AAV2와 아데노바이러스를 동시에 감염하는 종래의 생산법에 의한 효율성과 새로운 생산법, 즉 AAV2 감염 후 pHelper 플라스미드를 transfection 하는 방법을 통한 생산효율성을 비교하였고, HEK293과 293T를 생산세포주로 하여 시간에 따른 생산효율성도 분석하였다. 그 결과 AAV2와 pHelper DNA를 포함한 새로운 생산법은 기존의 방법에 비해 10배 이상 높은 생산효율성을 보였고, 293T에서 AAV2를 10 MOI로 감염한 후 5일째에 가장 높은 생산효율성을 보였는데, 생산세포 한개 당 1.61×10? virus genomes (v.g.)을 생산하는 결과였다. 따라서 이 생산조건을 다른 혈청형 생산에 적용한 결과, 모든 혈청형에서 생산세포주 한 개 당 10? v.g. 이상을 생산하는 효율성을 보였다. 한편, 다양한 AAV 혈청형을 한 가지 공통적인 방법으로 정량하기 위해 the universal PCR 프라이머를 제작하였고, 그것을 이용하여 신뢰성 높고 10개 분자까지도 증폭이 가능한 결과를 모든 혈청형에서 얻었다. 그러므로 이 한 쌍의 정량용 the universal 프라이머는 임상시험용 아데노바이러스벡터에 존재하는 AAV오염을 검출하는 것에도 사용 가능하다. Adeno-associated virus (AAV) has been considered to be a very safe and efficient gene delivery system. However, the major obstacles to therapeutic usage of AAV have been to achieve highly efficient and reproducible production processes, and also to develop a reliable quantifying method of various serotypes with a simple protocol. We compared the efficiency of the conventional production protocol of AAV2 and adenovirus (Ad) co-infection to that of a new method containing AAV2 infection followed by pHelper transfection. We tested HEK293 and 293T, and further examined the time-dependent changes of AAV2 production. The new method of AAV2 and pHelper DNA gave about ten times higher production efficiency than that of the conventional protocol. The highest production efficiency in 293T was achieved as 1.61 × 10? virus genomes (v.g.)/cell by the new method of 10 MOI of AAV2 infection and 5 days post-infection. This protocol of the highest efficiency was then applied to produce various AAV serotypes and showed the efficiencies higher than 10? v.g./cell. Next, we designed the universal PCR primers of highly conserved regions for various AAV serotypes to develop a simple and reliable titration method. The universal primers could amplify all the tested AAV serotypes with similar sensitivities by ten molecular copies. Therefore, this pair of universal primers can be further utilized to detect AAV contaminants in therapeutic adenoviral vectors.
초내열합금분말에 의한 PTA 오버레이부의 연삭 마모 특성 연구
김영식,최영국,임창훈,김종도,Kim, Young-Sik,Choi, Young-Gook,Lim, Chang-Hoon,Kim, Jong-Do 대한용접접합학회 2009 대한용접·접합학회지 Vol.27 No.3
The Plasma Transferred Arc (PTA) overlay welding method is lately introduced as one of the most useful surface overlay method of the engine component. In this paper, the overlay welding on the SNCrW heat resisting alloy was conducted by the PTA overlay welding process using the super alloy powder. The characteristics of the overlay layers were investigated through the metallurgical and abrasive test. Experimental results showed that the overlay on the SNCrW heat resisting alloy surface was successfully made without hot cracking. The friction wear characteristics of the Co-base Stellite 6 overlayer were most superior. However the abrasive wear characteristics were most inferior in the Co-base Stellite 6 overlayer.
Ni 계 초내열합금의 PTA 오버레이 층 특성에 관한 연구
김영식,최영국,이광렬,Kim Young-Sik,Choi Young-Goog,Lee Kwang-Ryeol 대한용접접합학회 2006 대한용접·접합학회지 Vol.24 No.3
The Plasma Transferred Arc (PTA) overlaying method is lately introduced as one of the most useful surface modification method of the engine component. In this paper, the characteristics of the Co-base and Ni-base super alloy overlay layers by PTA method were investigated through the metallurgical, abrasive and cavitation erosion test. Experimental results showed that the abrasive wear resistance of the Co-base Stellite 6 overlayer was the most superior and followed in order of Nimonic 80A, Inconel 625 and Inconel 718. However, the cavitation erosion characteristic of the Stellite 6 overlayer was the most inferior and it was better in order of Inconel 625, Inconel 718 and Nimonic 80A.