3T3-L1 지방 전구세포의 분화 및 지방 생성에 미치는 Fragaria Orientalis L. 물 추출물의 영향

최문열 ( Moon-yeol Choi ),김미형 ( Mi Hyung Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2024 大韓本草學會誌 Vol.39 No.3

Objective : Obesity, which has recently been rapidly increasing in the obese population, is caused by an imbalance in energy intake and consumption. The reason why we need to manage obesity well is that the prevalence of complications such as diabetes, atherosclerosis, insulin resistance, and cardiovascular disease increases. In this study, the effect of FO (Fragaria orientalis) water extract on fat metabolism in 3T3-L1 cells was observed to develop a new anti-obesity material based on Mongolian medical books. Methods : The effect of FO extract on adipogenesis in 3T3-L1 cells was observed using DPPH scavenging, pancreatic lipase inhibitory activity, MTT analysis and Oil-red-O staining method. And the expression of proteins related to lipid metabolism was analyzed by Western blot. Results : The FO group significantly increased the DPPH radical scavenging activity at 5 mg/ml compared to the positive control BHA at 0.1 mg/ml. In oil red O staining at a safe concentration without cytotoxicity, lipid accumulation was significantly inhibited by less than 80% compared to the control group at all concentrations. Moreover, treatment of FO significantly increased the expression of proteins related to lipid metabolism, such as p-AMPK and p-ACC, in 3T3-L1 cells, and the expression of CPT-1 tended to increase in a dose-dependent manner. However, the expression of PPAR-γ was significantly decreased in a dose-dependent manner. Conclusion : These results suggest that FO water extract has a potential anti-obesity effect and are expected to be utilized in the development of materials for obesity prevention and treatment.

미숙감귤의 항산화 및 항노화 활성에 대한 평가

최문열 ( Moon-yeol Choi ),박해진 ( Hae-jin Park ),김재수 ( Jae Soo Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.1

Objective : This study was designed to investigate effect of immature fruits from Citrus unshiu Markovich to mature them on antioxidative and antiaging-related enzyme activities in vitro. Methods : Fruits from Open field-cultivated C. unshiu Markovich (ripe and unripe fruits of Citri) made a purchase in Jeju island. We measured total polyphenol and flavonoid contents in mature C. unshiu Markovich (MC) and immature C. unshiu Markovich (IMC) 70% ethanol extract. DPPH free radical, ABTS radical, and hydroxyl radical scavenging activities in both extracts were determined. Also, reducing power, SOD-like activities, elastase inhibition, and collagenase inhibition activities of these extract were assayed at 5, 1, 0.5, and 0.1 ㎎/㎖. Results : The total polyphenol contents in the ethanol extract of IMC were higher than those of MC (26.99 ± 0.89 ㎎/TAEg). And total flavonoid contents in them were very similar to both extract (MC; 7.25 ± 0.4 ㎎/RUEg and IMC; 7.44 ± 0.18 ㎎/RUEg). DPPH free radical scavenging activities, ABTS radical scavenging capacity, reducing power, and SOD-like activities of IMC-treated group showed significant increase compared to those of MC group in all treated concentrations. Particularly, DPPH free radical scavenging and elastase inhibition activities in IMC-treated group at 5 ㎎/㎖ showed similar with positive control group. Besides, IMC extract at 5 ㎎/㎖ concentration surpassed positive control (BHA or Vit. C) in SOD-like activities and ABTS radical scavenging capacity. Conclusion : These results suggest that IMC ethanol extract may be used as a natural antioxidant and a antiaging material for development of functional foods and cosmeceuticals.

Fragaria Orientalis 물 추출물이 고지방식이를 급여한 C57BL/6 Mice에서 비만 관련 인자에 미치는 영향

최문열 ( Moon-yeol Choi ),김미형 ( Mi Hyung Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2024 大韓本草學會誌 Vol.39 No.4

Objective : In this study, the anti-obesity effect of Fragaria orientalis (FO) on high-fat diet-induced obese mice was investigated. Drug treatment methods are widely used as obesity treatment methods, but research using various natural products is being conducted due to safety concerns. This study aims to evaluate the anti-obesity effect of FO extract, a natural product derived from Mongolia. Methods : C57BL/6 mice were used and divided into three groups, normal diet group, high-fat diet group, and high-fat diet with FO oral treatment group at a dose of 300 mg/kg. Extract was orally provided everyday for 6 weeks. Body Weight and food intake were measured every 2 days and blood lipid profiles and liver function in the sacrificed mice were evaluated. In addition, protein expression in hepatic tissue and histomorphological changes in liver and adipose tissue were observed. Results : Body weight, adipose tissue weight and FER were significantly lower in a high-fat diet with FO treatment than fed only high-fat diet. There was a significant difference between the high-fat diet and the FO-treated high-fat diet mice. As a result of analyzing lipid metabolism-related genes in hepatic tissue, all of p-AMPK, p-ACC, PPAR-α, CPT-1, and UCP-1 showed significant increases, and PPAR-γ also decreased significantly compared to the high-fat diet group. Conclusion : Overall, these results indicate that FO is effectual in improving obesity, suggesting that it can be used as a possible material for anti-obesity agents or functional supplements for weight control.

우슬의 에탄올 추출물이 모발 성장에 미치는 영향

이미자 ( Mi-ja Lee ),최문열 ( Moon-yeol Choi ),김유진 ( Yoo Jin Kim ),김미려 ( Mi Ryeo Kim ),유왕근 ( Wang Keun Yoo ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.4

Objective : As more and more people are interested in appearance in modern society, the increasing number of hair loss population can have an important impact on psychological and social problems such as depression and inappropriate interpersonal symptoms. Therefore, much research is being done on treatments for alopecia using herbal extracts with relatively few side effects. This study was investigated about the effect of Achyranthis Radix (AR) extract with ethanol solvent on hair growth. Methods : We determined the promoting efficacy of AR-ethanol extract compared with minoxidil (MNXD) on the growth of human hair dermal papilla cells (HDPCs). Cell viability was measured by MTT assay and cell proliferation was confirmed by cell cycle analysis from flow cytometry in HDPCs. Also, we monitored the safe concentration range through MTT assay. And protein expression of hair growth-related genes (insulin-like growth factor 1 (IGF-1), Wnt3a, Protein kinase B (Akt), Extracellular signal-regulated kinase (Erk)) was monitored by western blot. Results : On cell cycle analysis, the G2/M phase was higher than that of the DW group in AR ethanol extract group at 0.05 and 0.1 ㎎/㎖. All protein expression levels of HDPCs were increased in AR ethanol extract groups and the MNXD group, compared to the DW group, respectively. Conclusion : As mentioned above, AR extract increased cell proliferation and the protein expression of IGF-1, Wnt3a, Akt, Erk in HDPCs. These results suggest that AR ethanol extract has promoted hair growth and it might be potential hair growth supplement.

Chamaeneron angustifolia 추출물이 3T3-L1 Preadipocyte의 지방대사 및 분화에 미치는 영향

이슬비 ( Seul Bi Lee ),최문열 ( Moon-yeol Choi ),김미형 ( Mi Hyung Kim ),김소영 ( So-young Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2024 大韓本草學會誌 Vol.39 No.4

Objectives : Obesity is an imbalance between energy intake and consumption due to overeating and lack of exercise, and if it persists, it increases non-infectious diseases such as high blood pressure, diabetes, and hyperlipidemia. In this study, we tried to investigate the possibility of using Chamaeneron angustifolia (CA) as a material for antiobesity by confirming the effect of inhibiting lipid differentiation. Methods : We measured the effects of CA extract on oil-red-o staining, cell cytotoxicity evaluation activity using 3T3-L1 cells. Additionally, we assessed fat decomposition and metabolism-related protein expression through Western blot analysis. Results : In this study, the anti-obesity effects of CA extract were experimentally assessed. Results showed significant inhibition of adipocyte differentiation and accumulation at concentrations of 0.05, 0.1, and 0.2 ㎎/㎖ of oil-red-o staining, with reductions of 80% or more. CA notably increased the phosphorylation of AMPK protein expression compared to the control group across all concentrations. Additionally, phosphorylation of ACC significantly increased at a concentration of 0.2 ㎎/㎖ compared to the control. PPAR-γ, which regulates adipogenesis, exhibited a significant decrease compared to the control, while protein expression of CPT-1, involved in fatty acid oxidation, showed a concentration-dependent increase across all groups. Therefore, CA extract demonstrates potential as a functional material for anti-obesity by increasing the expression of proteins related to fat decomposition and synthesis while decreasing others. Conclusions : These results suggest that CA may also be useful as an anti-obesity functional substance.

우슬의 에탄올 추출물이 모발의 흑화에 미치는 영향

이미자 ( Mi-ja Lee ),최문열 ( Moon-yeol Choi ),김유진 ( Yoo Jin Kim ),김미려 ( Mi Ryeo Kim ),유왕근 ( Wang Keun Yoo ) 대구한의대학교 제한동의학술원 2021 東西醫學 Vol.46 No.1

Objective : a-MSH (alpha-melanocyte-stimulating hormone) and IBMX (3-lsobutyl-1-methylxanthine) were known as regulating melanogenesis and differentiation, but they were restricted usage because of stability. So, natural products have developed about regulating melanogenesis and differentiation. Therefore, we investigated about effect of Achyramthis Radix (AR) ethanol extract on gray hair. Methods : a-MSH was used as a positive control. We set up the safe concentration range by MTT assay and conducted melanin contents and tyrosinase activity in mouse melanoma (B16F10) cells. Results : As results, melanin contents were increased in Achyramthis Radix ethanol extract (AR) group contrast to control group. Also, AR group is higher than control group on tyrosinase activity. Conclusion : Our results suggest that AR could be used as a potential material for the treatment of gray hair by increasing the melanogenesis of B16F10 cells.

추출 용매를 달리한 생강 추출물에 대한 생리활성의 비교 평가 연구

김소영 ( So Young Kim ),최문열 ( Moon-yeol Choi ),도은주 ( Eun Ju Do ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.2

Objective : This study was designed to investigate anti-oxidative, anti-aging and anti-inflammatory activities of Zingiber officinale Roscoe water (ZD) and 50% ethanol extracts (ZE). Method : The antioxidant capacity of ZD or ZE was analyzed by DPPH, ABTS scavenging activities and reducing power, respectively. The anti-aging efficacy was performed with tyrosinase and collagenase inhibition activities. The anti-inflammatory activities were confirmed through inhibition of nitric oxide production and TNF-α expression in LPStreated Raw 264.7 cells. Result : As a result of measuring DPPH and ABTS radical scavenging activities, both ZD and ZE extracts increased in concentration-dependent manners. The ABTS radical scavenging activities of water and 50% ethanol extract were similar to that of positive control at high concentration. On reducing power, both ZD and ZE showed higher activities than the positive control at high concentration. On inhibition activities of tyrosinase and collagenase both ZD and ZE showed the large inhibition rates at low concentration. Also, the inhibitory efficacy of ZD on NO and TNF-α production was better than ZE in RAW 264.7 cells. Conclusion : These results suggest that ZD and ZE have anti-oxidative, anti-aging and anti-inflammatory effects in vitro. Especially, ZD was more effective than ZE. Thus, Ginger extract is considered to be highly useful as a cosmetic or food material with anti-oxidative, anti-aging (prevention of whitening and wrinkle) and anti-inflammatory effects. Also these effects may vary depending on the extraction solvent.

한약재 함유 대두발효 추출물이 streptozotocin으로 유발된 당뇨 흰쥐의 혈당 및 조직형태학적 변화에 미치는 영향

조창숙 ( Chang Suk Jo ),김소영 ( So Young Kim ),최문열 ( Moon-yeol Choi ),김미형 ( Mi Hyung Kim ),김미려 ( Mi Ryeo Kim ),서부일 ( Bu-il Seo ) 대한본초학회 2022 大韓本草學會誌 Vol.37 No.1

Objective : This study was designed to investigate anti-diabetic effects of fermented soy bean extract with herbal medicines (Godjang) in diabetic rat models induced by streptozotocin (STZ) injection. Method : Changes in body weight, drinking water, and food intake were observed for 4 weeks before and after induction of diabetes mellitus in rats. The anti-diabetic capacity of Godjang was analyzed by fasting blood glucose (FBG) every week. Also, after 4 weeks of administration, the oral glucose tolerance test (OGTT) was performed, and then blood levels of insulin were checked. And serum levels of total cholesterol and triglycerides were determined. Histomorphological changes of liver, kidney and pancreatic tissues were also observed in STZ-induced diabetic rats and Godjang administered rats. Result : In Godjang administered group, body weight and water intake were more lower than that of STZ-induced diabetic rats. FBG was decreased in the Godjang administered group than STZ-induced diabetic group. According to OGTT, blood glucose levels at 30 minutes and 60 minutes significantly decreased in Godjang administered group than in STZ-induced diabetic control group. Administration of Godjang extract for 4W significantly decreased levels of serum glucose, total cholesterol (TC), triglycerides (TG) in diabetic rats. In histomorphological analysis of kidney, liver, Godjang administrated groups showed the inhibition of pathological damage. Conclusion : These results suggest that Godjang extract has an anti-diabetic action through decrease in serum glucose, TC, TG levels and recovery of the morphological changes in kidney and liver in STZ-induced diabetic rats.1)

제1형 당뇨모델 흰 쥐의 간과 신장에 미치는 한약재 첨가 콩 발효식품의 영향

조창숙 ( Chang Suk Jo ),김소영 ( So Young Kim ),최문열 ( Moon-yeol Choi ),김미형 ( Mi Hyeong Kim ),김미려 ( Mi Ryeo Kim ),서부일 ( Bu-il Seo ) 한약응용학회 2021 한약응용학회지 Vol.21 No.1

Objective : Diabetes mellitus (DM) is a chronic metabolic disorder characterized by high blood glucose levels due to the relative or absolute deficiency of insulin action, and has become a major public health and economic problem worldwide. Godjang (GJ) was made by fermentation of soybean with herbal medicine. The purpose of this study was to investigate the effects of Godjang (GJ) water extracts on antidiabetic markers in streptozotocin (STZ, 65 mg/kg of BW, IP injection)-induced type 1 diabetic rats. Methods : Experimental DM was induced by intraperitoneal injection with a single dose of STZ (65 ㎎/㎏) in Sprague Dawley rats. In other words, animals were divided into 4 groups; NG (normal group + 0.9% saline), COS (diabetic group + 0.9% saline), GJ 200 (diabetic group + Godjang (200 mg/ kg, BW)), GJ 600 (diabetic group + Godjang (600 ㎎/㎏, BW)). Treatments were performed orally administration for 4 weeks. After the experiment of 4 weeks, the blood samples and organs were collected and then serum levels of glucose, BUN, creatinine, TC, TG, ATS and ALT were measured, respectively. Results : At the 4 weeks of GJ administration, the body weight gain were lower in diabetic groups than normal group. The serum glucose levels were significantly lower in the GJ 200 and 600 group than COS group. In serum, AST and TG levels significantly decreased in GJ 600 group compared to COS group. The serum levels of ALT, TC, BUN and creatinine were decreased in GJ 200 and 600 groups compared to COS group. Conclusion : Our results suggest that Godjang water extracts would have antidiabetic effects in STZ-induced diabetic rats.