Biochemical Characteristics of the Purified Pea Chloroplast Fructose-1,6-bisphosphatase

조만호,한태룡,Cho, Man-Ho,Hahn, Tae-Ryong 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

완두잎으로부터 엽록체 fructose-1,6-bisphosphatase를 분리하여 특성에 관하여 연구하였다. 분리한 효소의 monomer 분자량은 SDS polyacrylamide gel electrophoresis 상에서 약 48,000으로 계산되었다. Sephadex G-200 gel permeation 크로마토그래피를 통해 결정된 intact 효소의 분자량은 약 160,000이었다. 그러므로 이 효소는 4개의 같은 subunit로 구성된 tetramer라고 생각된다. 이 효소는 중성 pH에서는 활성이 없었으나 알카리 pH에서는 활성을 가진다. 또한 이 효소는 $Mg^{2+}$에 의해 활성화 되었으며 dithiothreitol과 같은 환원제에 의해서도 활성화되었다. 세포질속에 있는 gluconeogenic fructose-1,6-bisphosphatase는 중성 pH에서 활성을 가지며 $Mg^{2+}$나 환원제의 영향을 받지 않는 것으로 알려져 있다. 이 효소는 세포질 fructose-1,6-bisphospha-tase의 inhibitor인 AMP에 대해 민감하지 않았다. $Mg^{2+}$에 대한 포화곡선은 S자 형태를 보이며, Hill coefficient는 3.3이었다. 효소반응의 활성화에너지$(E_a)$와 Arrhenius frequency factor (A)는 각각 21.5 kcal/mol과 $4.01{\times}10^8/s$였으며, 활성화엔트로피$({\Delta}S^{\neq0})$와 활성화엔탈피$({\Delta}H^{\neq0})$는 각각 -19.7cal/$K{\cdot}mol$과 20.87kcal/mol이었다. Chloroplast fructose-1,6-bisphosphatase from pea leaves was purified and characterized. Monomeric molecular weight of the enzyme was estimated a s 48,000 by SDS polyacrylamide gel electrophoresis. Molecular weight of the intact enzyme as determined by Sephadex G-200 gel permeation chromatogrphy was about 160,000. Thus the enzyme is believed to be tetrameric and composed of four identical subunits. The purified chloroplast enzyme was inactive at neutral pH but active at alkaline pH. The enzyme was also activated by $Mg^{2+}$ and reducing agents such as dithiothreitol. Gluconeogenic fructose-1,6-bisphosphatase in cytosol is known to be active at neutral pH and insensitive to $Mg^{2+}$ and reducing agents. The enzyme was insensitive to AMP which is a potent inhibitor for cytoplasmic fructose-1,6-bis-phosphatase. The saturation curve of $Mg^{2+}$ shows sigmoidal shape and Hill coefficient was 3.3. Activation energy $(E_a)$ and Arrhenius frequency factor (A) of the enzyme catalyzed reaction were 21.5 kcal/mol and $4.01{\times}10^8/s$, respectively. Activation entropy $({\Delta}S^{\neq0})$ and activation enthalpy $({\Delta}H^{\neq0})$ of the enzyme reaction were -19.17 cal/$K{\cdot}mol$ and 20.87 kcal/mol, respectively.

분리된 완두 엽록체 Fructose - 1 , 6 - bisphosphatase 의 생화학적 특성

조만호,한태룡 ( Man Ho Cho,Tae Ryong Hahn ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

Chloroplast fructose- 1,6-bisphosphatase from pea leaves was purified and characterized. Monomeric molecular weight of the enzyme was estimated as 48,000 by SDS polyacrylamide gel electrophoresis. Molecular weight of the intact enzyme as determined by Sephadex G-200 gel permeation chromatogrphy was about 160,000. Thus the enzyme is believed to be tetrameric and composed of four identical subunits. The purified chloroplast enzyme was inactive at neutral pH but active at alkaline pH. The enzyme was also activated by Mg^(2+) and reducing agents such as dithiothreitol. Gluconeogenic fructose-1,6-bisphosphatase in cytosol is known to be active at neutral pH and insensitive to Mg^(2+) and reducing agents. The enzyme was insensitive to AMP which is a potent inhibitor for cytoplasmic fructose-1,6-bisphosphatase. The saturation curve of Mg^(2+) shows sigmoidal shape and Hill coefficient was 3.3. Activation energy (E_a) and Arrhenius frequency factor (A) of the enzyme catalyzed reaction were 21.5 kcal/mol and 4.01×10^8/s, respectively. Activation entropy (△S^(≠0) and activation enthalpy (△H≠0) of the enzyme reaction were -19.17 cal/K·mol and 20.87 kcal/mol, respectively.

형질전환된 E . Coli LE392 로 부터 Plasmid - encoded thioredoxin 의 분리정제 및 특성

조만호,한태룡 ( Man Ho Cho,Tae Ryong Hahn ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1

한국산 유색미의 주요성분인 Cyanidin 3 - glucoside 의 수용액에서의 열안정성

조만호(Man Ho Cho),윤혜현(Hye Hyun Yoon),한태룡(Tae Ryong Hahn) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.3

Thermal stability of the major color component, cyanidin 3-glucoside, isolated from Korean pigmented rice (Oryza sativa var. Suwon 415) were investigated to explore possible application of value-added natural colors as fad additives. The anthocyanin showed red and blue color with maximum absorption peaks at 511 ㎚ and 572 ㎚ in acidic (pH 2.0) and alkaline (pH 9.0) buffer solutions, respectively, and the thermal degradation reactions were carried out with different temperature ranges at 50∼90℃. Degree of degradation was determined with UV/Vis spectra which indicate characteristic absorption patterns with sharp isosbestic points at 350 ㎚ (pH 2.0), and 275, 310, and 405 ㎚ (pH 9.0) Thus the reaction follows simple first-order kinetics. The anthocyanin was very stable against heat at acidic pH and relatively stable at alkaline pH with half-life values of 50.3 hr and 0.6 hr at 70℃, respectively. The activation energies and Arrhenius frequency factors of the pigment were 26.9 ㎉ ㏖^(-1) and 6.0×10^11 s^(-1), at pH 2.0, and 15.2 ㎉ ㏖^(-1) and 1.4×10^6 s^(-1), at pH 9.0, respectively.

한국산 유색미에서 분리한 안토시아닌의 화학구조

조만호(Man Ho Cho),백영숙(Young Sook Paik),윤혜현(Hye Hyun Yoon),한태룡(Tae Ryong Hahn) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.4

The major color component of a Korean pigmented rice (Oryza sativa var. Suwon 415) was purified with Amberlite XAD-7 column and preparative paper chromatography. The purified pigment was determined as anthocyanin by paper chromatography, UV/Vis and NMR spectroscopy. The λ_(max) of the purified anthocyanin on UV/Vis spectrum were 529 ㎚ and 281 ㎚. The A_(440)/A_(525) value of the purified anthocyanin was 23% suggesting the presence of 3-glycosidic structure. The aglycone from acid hydrolysis showed bathochromic shift (18 ㎚) in the presence of AlCl₃ indicating that the anthocyanidin contained free adjacent hydroxyl groups such as cyanidin, delphinidin, petunidin or luteolinidin. The sugar moiety obtained from acid hydrolysis was determined as glucose by paper chromatography. The NMR spectra showed that the aglycone was cyanidin and the sugar was β-D-glucopyranose. Thus, the chemical structure of the purified anthocyanin was identified as cyanidin 3-O-β-D-glucopyranoside.

Neural Network 알고리즘을 이용한 용접공정제어

조만호(Man Ho CHO),양상민(Sang Min Yang) Korean Society for Precision Engineering 2004 한국정밀공학회지 Vol.21 No.12

A CCD camera with a laser stripe was applied to realize the automatic weld seam tracking in GMAW. It takes relatively long time to process image on-line control using the basic Hough transformation, but it has a tendency of robustness over the noises such as spatter and arc light. For this reason, it was complemented with adaptive Hough transformation to have an on-line processing ability for scanning specific weld points. The adaptive Hough transformation was used to extract laser stripes and to obtain specific weld points. The 3-dimensional information obtained from the vision system made it possible to generate the weld torch path and to obtain the information such as width and depth of weld line. In this study, a neural network based on the generalized delta rule algorithm was adapted for the process control of GMA, such as welding speed, arc voltage and wire feeding speed.

이동목욕(移動沐浴)서비스 수혜노인(受惠老人) 만족도(滿足度) 조사(調査)

임춘식(Choon Seek Rim),조만호(Man Ho Cho) 한국노인복지학회 2000 노인복지연구 Vol.9 No.-

This study was intended to make a comparative analysis of the living attitude of the beneficiary from the bathing service of the mobile bath car effected by the Pyounghwa Comprehensive Social Well-being Center located in Nowon-ku, Seoul. The individual in-depth research method, of qualitative research methods, was used as the research method. An attempt was made to present an realistic alternative to activating our mobile bathing service project in infancy into the productive bathing well-being one as follows: First, it is necessary to expand and implecrnt the mobile bathing project because the periodic and continual implementation of the mobile bathing service project can improve the sanitary state of the patients afflicted with palsy and dementia and mitigate the burden of their family. Second, it is necessary to consider the plan to raise the efficiency of the mobile bathing service project. Third, sanitation management and specialized educational activity should be systematically provided at a time of mobile bathing service. Fourth, mobile bathing service can be thought to be a kind of health nursing service and especially, comfortable and safe bath and nursing for patients may be the important project. Fifth, it is necessary to consider the scheme to establish the bathing facility within the social well-being facility to enhance the efficiency of mobile bathing service and provide bathing service with mobile bathing vehicle service. Sixth, it is necessary to organize the(tentatively called) mobile bathing well-being research institute composed of authoritative scholars and specialists from the medical community and the social well-being community with an aim of activating our mobile bathing service in infancy, enhancing the qualitative level of service and further, spreading and improving bathing well-being.

천연색소로서 한국산 유색미 안토시아닌의 안정성 연구

윤주미(Joo-Mi Yoon),조만호(Man-Ho Cho),한태룡(Tae-Ryong Hahn),백영숙(Young-Sook Paik),윤혜현(Hye-Hyun Yoon) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.2

Geniposide 로부터 Genipin 으로 생변환을 위한 β-Glucosidase 의 고정화

박성길(Sung Gil Park),조만호(Man Ho Cho),이윤형(Youn Hyung Lee),한태룡(Tae Ryong Hahn),정인식(In Sik Chung) 한국산업식품공학회 2000 산업 식품공학 Vol.4 No.2

N/A

홍화적색소 Carthamin 의 효과적인 분리 및 화학구조 분석

김준범(Jun Beom Kim),조만호(Man Ho Cho),한태룡(Tae Ryong Hahn),백영숙(Young Sook Paik) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.6

The major red color component from Carthamus tinctorius was efficiently isolated by a modified new method of traditional procedure and column chromatographic technique. After removing yellow pigments by washing Carthamus flowers with water and methanol, the red pigment was extracted with 0.5 M Na₂CO₃, and precipitated with aqueous citrate. The pigment was further isolated and purified with cellulose adsorption and Sephadex LH-20 column chromatography. The purified red pigment was decomposed at about 300℃, and its R_f value on silica gel in BAW(n-BuOH : HOAc : H₂O=4 : 1 : 5) was 0.56. UV/Vis spectrum of the red pigment showed λ_(max) at 519, 372, 311, 244 ㎚ and its IR spectrum showed wide and strong absorption near 3400 cm(-2) indicating characteristic hydroxyl groups. ¹H and ^(13)C NMR spectra showed that it contained two enolized β-triketone, two p-hydroxycinnamoyl moieties, a methine, and two glucosyl moieties. On the basis of spectroscopic evidences, the chemical structure of the red pigment was identified as 6-β-D-glucopyranosyl-2-[[3-β-D-glucopyranosyl-2,3,4-trihydroxy-5-[3`-(4$quot;-hydroxyphenyl)-1`-oxo-2`-propenyl]-6-oxo-1,4-cyclohexadien-1-yl]methylene]-5,6-dihydroxy-4-[3`-(4$quot;-hydroxyphenyl)-1`-oxo-2`-propenyl]-4-cyclohexene-1,3-dione, carthamin.