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돼지의 UCP3 유전자의 단일염기서열 변이와 경제형질과의 연관성 분석
Jae-Don Oh(오재돈),Kun-Woo Lee(오건우),Il-Jung Jung(정일정),Gwang-Joo Jeon(전광주),Hak-Kyo Lee(이학교),Hong-Sik Kong(공홍식) 한국생명과학회 2011 생명과학회지 Vol.21 No.1
Uncoupling protein (UCP) 3 유전자는 갈색지방세포의 미토콘드리아 내막에 존재하며 탈공역 산소(uncoupling oxygen)를 통해 ATP를 생산하는 것으로 알려져 있다. 이는 세포 내의 과다 에너지를 열로 발산시키는 기능을 하고 있다. 본 연구는 돼지의 UCP 3 유전자 내 missense mutation의 유전자형을 조사하고 경제형질과의 연관성을 분석하기 위하여 실시하였다. 돼지의 UCP3 유전자의 염기서열 분석을 통해 1405 bp 지역에서(accession number: AY739704) G염기가 A염기로 치환되는 변이를 확인하였다. 확인된 변이지역은 G가 A로 치환됨으로 인해 150번째 아미노산 서열이 glycine (GGG)에서 arginine (AGG)으로 바뀌는 missense mutation임을 확인하였다. 각 유전자형의 빈도는 0.164(GG), 0.587(GR) 그리고 0.249(RR)로 확인되었으며, 각 대립유전자의 빈도는 0.458(G)과 0.542(R)로 확인되었다. 돼지 UCP3의 G150R 유전자형과 경제형질 간의 연관성을 분석한 결과 등지방두께에 있어 유의적인 연관성이 검출되고 일당증체량과 90 kg 도달일령에서는 유의적인 값이 검출되지 않았다. Uncoupling protein (UCP) 3 has a number of proposed roles in the regulation of fatty acid metabolism. A number of polymorphisms in the human UCP3 gene have been identified, and the correlation with obesity related phenotypes evaluated. The objective of this study was to identify SNP in porcine UCP3 gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms at position 1405 bp (Genebank accession No : AY739704) in porcine UCP3 gene. The SNP (G150R), located in the exon 3, changed the amino acid to glycine (GGG) from arginine (AGG). This G150R showed three genotypes - GG, GR and RR - by digestion with the restriction enzyme Sma Ⅰ using the PCR-RFLP method. The G150R showed significant effects only on back fat (P<0.05). Animals with the genotype GG had significantly higher back fat thickness (1.358 ㎝) than animals with the genotype GR (1.288 ㎝, P<0.05) and RR (1.286 ㎝, P<0.05). However, the genotypes had no significant association with ADG and days to 90㎏. According to results of this study, a G allele of the G150R was found to have a significant effect on back fat thickness. It will be possible to use SNP markers on selected pigs to improve backfat thickness, an important economic trait.
동물조직에서 실시간 유전자 추출을 위한 소형기기의 설계 및 제작
이창근(Chang-Geun Lee),임희택(HeeTaek Lim),윤두학(Duhak Yoon),정일정(Il-Cheong Cheong),정효일(Hyo-Il Jung) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Here, we present a new method and equipment for the extraction of DNA from animal tissues. Our equipment has been designed based on a conventional biochemical analysis system and consists of three major parts; sampling, flow-centrifugation and DNA filtration part. The screw of the sampling part can collect a small piece of tissues from a lump of meat. The piece of meat was subjected to a specially designed tube to extract DNA. The lysed cells mixture were then loaded to the flow-centrifuge part. After the DNA filtration, the elution buffer and cylinder pressure could produce the clean DNA. It is expected in near future that our equipment will help identify the Korean cattle as well as genetically modified organisms.
한국 재래돼지 브랜드 돈육 원산지 검증을 위한 유전자 감식 기법 활용 연구
최봉암 ( Choi Bong-am ),이학교 ( Lee Hak-kyo ),전광주 ( Jeon Gwang-joo ),오재돈 ( Oh Jean-don ),최일신 ( Choi Il-sin ),박미현 ( Park Mi-hyun ),공홍식 ( Kong Hong-sik ),정일정 ( Jung Il-jung ),김태헌 ( Kim Tae-hun ),윤두학 ( Yoon D 한국유기농업학회 2004 韓國有機農業學會誌 Vol.12 No.2
Identification of animals has been used with an ear tag with dummy code and blood typing has been used for paternity and individual identification in live animals. Various genetic markers are different for breeds of pig and hence, it is necessary to identity the discrete genetic marker in korean native pig. A total of 240 pigs were used to find korean native pig population specific markers that expressed in population of korean native pigs. To identify the individual traceability, 20 animals were randomly chosen and tested for a whole process from being live to slaughter stages. The candidate genetic marker used in the study were 18 DNA microsatellites which were identified in pig genome. The number of alleles of those DNA microsatellites ranged form a minimum of 3 to maximum of 6. The heterozygote frequency ranged from 0.44 to 0.69. Effective number of alleles for each DNA microsatellotes were 2 to 4. By choosing 6 candidate genetic markers among all, the traceability of individual identification was estimated as accurate as 99.99%(p>0.0014), nearly.