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정인애,강종백,Chung, In-Ae,Gang, Jong-Back Korean Society of Life Science 2002 생명과학회지 Vol.12 No.2
온도 기울기 전기영동장치를 이용하여 d(CXG)와 d(GXC) 염기의 열 안정성을 결정하는데 사람의 $\varepsilon$-글로빈 DNA조각을 사용하였다. 염기 쌍의 안정성은 이웃하는 염기서열에 의한 수소결합과 base stocking 상호작용에 의존한다. 염기 쌍의 안정성은 d(CXG) d(CYG)의 경우에 T.A<A.T<C.G<G.C이고 또한 d(GXC).d(GYC)의 경우는 A.T<T.A<C.G<G.C 이다. 그리고 d(GXC).d(GYC)의 경우에 mismatch 염기 쌍의 안정성은 G.T=T.G>G.A = A.G>C.T>T.C>C.A>A.C이다. Human $\varepsilon$-globin DNA fragment was used to determine the thermal stabilities of base pairs at d(CXG) and d(GXC) by Temperature Gradient Gel Electrophoresis(TGGE). The base pair stability depends on the hydrogen bonding interaction and base stacking interaction of neighbor base sequence. The orders of base pair stabilities were T.A<A.T<C.G<G.C for d(CXG).d(CYG) and A.T<T.A<C.G<G.C for d(GXC).d(GYC). And also heteroduplex TGGE suggested that the mismatch bases stabilities were G.T = T.G>G.A = A.G>C.T>T.C>C.A>A.C for d(GXC).d(GYC).
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정인애(In Ae Chung),신동식(Dong Sik Shin),정병수(Byoung Soo Chung),고익태(Ik Tae Koh) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.5
We report a case of cerebriform intradermal nevus. a cause of cutis verticis gyrata that clinically they resembled neurofibroma and revealed histopathologic finding consistent with intradermal nevus. The patient was a 6 year-old boy, who had a 17x18cm, soft, convoluted. pigmented mass over the parietal and occipital regions. The lesion had been present since birth and had been slowly increasing in size with convolution.
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사람 태아 피부의 S - 100 단백 염색상에 관한 연구
정병수(Byung Soo Chung),정인애(In Ae Chung),황선욱(Sun Wook Hwang) 대한피부과학회 1989 大韓皮膚科學會誌 Vol.27 No.1
The authors attempted to examine the distribution of S-100 protein in the human fetal skin. Immunohistochemical staining(ABC rnethod) using anti-S-100 antibodies was carried out on skin specimens taken from 11 human fetuses ranging from 9 weeks to 27 weeks of estimated gestational age. At 9 weeks of estimated gestational age, the embryonic epidermis consisted of three cell layers,' the basal layer, intermediate layer and periderm, all of them being stained for S-100 protein. But after 18 weeks, the basal layer changed to be negative. Granular and cornified layer's, beginning their development at 22 weeks, were not stained for S-100 protein. Hair germ of 12 week-fetuses was recognized unstained as a bulge of basal cells. In fully differentiated structural components of the hair follicle after 18 weeks, the outer root sheath only was stained for S-100 protein whereas the inner root sheath, hair matrix cells and sebaceous glands were unstained. Eccrine gland germs developed at 12 weeks of embryonic life as undulation of the basal layer and were not, stained. And at 22 weeks, the secretory portion of the eccrine glands were formed in the dermis and stained for S-100 protein. Our present study suggests that the expression of S-100 protein can undergo considerable changes during ernbryonic differentiation in the epidermis and epidermal appendages.
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김경진(Kyung Jin Kim),정인애(In Ae Chung),정병수(Byoung Soo Chung),최규철(Kyu Cherl Choi) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.1
A 35-year-old female patient had a cyst-Jike swelling for one month on the right scaphoid fossa and antihelix of the auricle. Histopathologically, cystic cavity was located in lower dermis and cavity roof was composed of fibrous material without cartilagenous structure. Treatrment consisted of incision and drainage and subsequently rnastoidectomy dressing for one week. One month after treatment, recurrence of lesion was not found.
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김경진(Kyung Jin Kim),정인애(In Ae Chung),최연홍(Youn Hong Choi),정병수(Byoung Soo Chung) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.2
Partial unilateral lentiginosis(PUL) is a rare pigmentary disorder charaeterized by lentigines limited to one side of the body with or without neurologic abnormalities. We report two cases of PUL unassociated with any other defect, One patient was 20-year-old female who had lentigines confined to the right side of the face and the other was 19-year-old male who had lentigines confined to the left side of the neck and upper trunk and left upper extremity. Histologic examination of the pigmented lesion revealed findings consistent with lentigo simplex.
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고압처리 원유로 제조한 발효유의 저장 중 유산균 수 변화와 이화학적 특성
안성일(Sung-Il Ahn),정인애(In-Ae Chung),정운시(Woon-Si Chung),주진우(Jin-Woo Jhoo),김거유(Gur-Yoo Kim),전정태(Jung-Tae Jeon) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.6
본 연구에서는 고압처리(high pressure processing) 한 원유를 이용하여 발효유를 제조하고, 발효특성 및 저장 기간 물리 화학적 특성의 변화를 살펴보았다. 고압처리 한 원유를 이용하여 발효유 제조 시 발효 초반에 유산균이 급격히 증식됨을 확인하였으나, 발효 완료 후 총 유산균 수는 대조군과 크게 차이가 나지 않았다. 저장 기간 중 실험군과 대조군 모두 유산균 수가 서서히 감소하는 경향을 나타내었다. 또한 산도 및 pH는 각 군 모두 정상적인 발효유의 범위를 나타내었다. 각 군의 점도를 대조해 본 결과 대조군이 실험군보다 점도가 높게 나타났으나, syneresis는 450 MPa 처리군이 대조군보다 유의적으로 적게 발생한 것으로 나타났다(P<0.05). 따라서 적절한 압력을 이용한 원유의 고압처리는 발효유의 물성을 개선하는 데 효과가 있다고 할 수 있으며, 이 분야에 관한 연구가 좀 더 이루어져야 할 필요가 있다. This study was carried out to investigate the physicochemical and fermentation properties of yogurt made from high pressure processing (HPP) treated milk. Raw milk and commercial yogurt starter were used to make yogurt. Raw milk was HPP treated at 350 or 450 MPa (HPP 350 or 450) for 15 min or heat treated at 80°C for 10 min. The numbers of lactic acid bacteria of the HPP treated group (HPP yogurt) rapidly increased during 2∼4 h, whereas there was not significant difference from control (P<0.05). Titratable acidity of all samples increased, and pH decreased during storage from 0.99 to 1.24%, as well as from 4.59 to 4.20, respectively. It is confirmed that these values are in general ranges for yogurt. Control showed higher viscosity than HPP 350. Syneresis was significantly lower than that of the control (P<0.05). Based on the data obtained from the present study, HPP treatment was effective to enhance the quality of yogurt.
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