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RISS 인기검색어
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- 저자 : 전황주 ( Hwang Ju Jeon ) (검색결과 4 건)
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Toxicological evaluation of organic contaminants in aquatic system using zebrafish embryo
Hwang-Ju Jeon(전황주),Hyeseung Choe,Chaeun Kim,Sung-Eun Lee 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
A threat to living organisms resulting from climate change on this planet leads to growing concern about the environment. One of these parts was Agriculture originated organic pollutants, known as a group of environmental contaminants. These contaminants cause many negative effects and even death on living organisms including aquatic vertebrates. But, many toxicological studies were still focused on their viewpoint only on the death problem. In this phenomenon, we tried to explore the possible toxicological effects of EPN on the early developmental stage of aquatic vertebrates using zebrafish embryos. In the acute toxicity test of AB strain zebrafish embryo, both pericardial and yolk sac edemas were observed at 500 ppb EPN treated group. Moreover, the presence of 250 ppb EPA makes zebrafish embryo obtain hematoma near the heart. To confirm phenotype of zebrafish more clearly, three different transgenic line were used including Tg[fli1a:EGFP], Tg[cmlc2:EGFP], and Tg[gata1:GFP]. In transgenic zebrafish, the malformation of the embryos was still observed. Especially, in the fluorescent image of Tg[cmlc2:EGFP], the formation of the heart seemed to be disturbed seriously. In the real-time qPCR data, the level of the transcripts related to early-stage heart formation, nppa, and myl7, were up and down-regulated to 2.0 and 0.26-fold respectively. In addition, the heart rate of the embryo was decreased at 250 and 500 ppb. Taking all these results together, EPN has the possibility of negative effects on the early-stage development of aquatic vertebrates including hematoma, malformation of the heart, and abnormal development of the internal circulatory system. Therefore, continuous monitoring and proper management are required.
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04 포스터 발표 : 환경 화학 분야(PC) ; PC-10 : 농경지 토양 중 PCB의 분포
전황주 ( Hwang Ju Jeon ),구본화 ( Bon Hwa Ku ),남태훈 ( Tae Hun Nam ),김용찬 ( Yong Chan Kim ),이지형 ( Ji Hyoung Lee ),최재일 ( Jae Il Choi ),이성은 ( Sung Eun Lee ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-
Polychlorinated biphenyl (PCB) compounds are persistent and hazardous to the environment. They are frequently found in industrial areas because their uses were bound to industrial purposes. However, it has been reported that PCBs can be translocated to agricultural soils via various ways. Therefore, PCBs are also found in agricultural foods including crops. PBCBs are consisted of 209 congeners and analyzed by GC-ECD, GC-MS and HRGC-HRMS. In this study, 27 PCB congeners were used to monitor PCBs in agricultural soil samples collected in Chungcheong and Gyeonggi Provinces. Prior to analyze soil samples, method validations were undertaken to know precision, accuracy, and recovery using #209 PCB congener (DeCB1, decachlorobiphenyl) followed by a method certified Environment Protection Agency. Those validation results were acceptable and all procedures containing soxhlet extractions and column chromatography for clean-up were adopted for analysis. All soil samples were analyzed for their basic properties including soil organic contents and soil pH. All soil samples were undertaken by soxhlet extraction for 16 h. Silicagel column chromatography was employed to clean up the samples and the final eluate was evaporated and then redissolved in toluene after complete dryness. The samples were analyzed using GC-ECD. Some of PCB congeners were found in these analyses and finally needs to be confirmed by GC-Ms and HRGC-HRMS.
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충청지역 농업용수 중 농약 유래 POPs의 모니터링 연구
이화성 ( Hwa Sung Lee ),전황주 ( Hwang Ju Jeon ),이회선 ( Hoi Seon Lee ),이성은 ( Sung Eun Lee ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.4
잔류성유기오염물질(POPs)은 현재 사용이 금지된 유기염소계 농약을 포함하고 이들은 국내 농업환경 중 발견이 되며 이들의 작물로의 이동 또한 보고되고 있다. 본 연구는 충청도 지역 15곳의 농업용수를 채취하여 이들 시료 중 POPs를 모니터링 하고자 하였으며 이들 시료는 액액분배를 통한 추출 및 정제컬럼을 이용한 정제과정을 거쳐 기체크로마토그래피에 의하여 분석 되었다. 농약유래 POPs 중 α-endosulfan이 0.01-1.13 ng/mL 수준에서 검출되었으며, β-endosulfan과 endosulfan sulfate는 각각 0.01-0.55 ng/mL 및 0.13-1.13 ng/mL 수준에서 검출되었다. 총 endosulfan 검출량은 0.38-1.18 ng/mL이었다. 현재 사용중인 농약 중 triadimefon 은 2.03-2.05 ng/mL 수준에서, tolyfluanid은 0.26-0.33 ng/mL 수준에서, chlorpyrifos는 1.34-3.85 ng/mL 수준에서 검출되었다. 농약계 POPs 중 endosulfan류의 물질들의 잔류가 확인되었고 이들의 환경 중 지속적인 모니터링이 필요하다. Persistent organic pollutants (POPs) including banned organochlorine pesticides, are found in Korean agricultural environments. Their translocation from agricultural environment to major crops has been reported. In this study, the POPs in 15 different agricultural waters of the Chungchung Province were monitored and all samples were prepared, followed by liquidliquid fractionation and column chromatography prior to analysis using gas chromatography. Among pesticide-originated persistent organic pollutants, α-endosulfan was determined in the range of 0.01 to 1.13 ng/mL. β-Endosulfan was determined in the range of 0.01 to 0.55 ng/mL, and endosulfan sulfate was ranged from 0.13 to 1.13 ng/mL. The total sum of endosulfans reached the range of 0.38 to 1.18 ng/mL. Three pesticides being used currently were also found in samples as 2.03-2.05 ng/mL for triadimefon, 0.26- 0.33 ng/mL for tolyfluanid, and 1.34-3.85 ng/mL for chlorpyrifos. Taken together, endosulfans were introduced in the agricultural waters and their fates need continuous monitoring.
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모델식물 애기장대에 대한 훈증제 메틸브로마이드의 약해발생 및 N-acetyl-L-cysteine, L-glutathione, indole-3-acetic acid의 약해억제 효과
김경남,김채은,박정은,유진성,김우성,전황주,김준란,이성은,Kim, Kyeongnam,Kim, Chaeeun,Park, Jungeun,Yoo, Jinsung,Kim, Woosung,Jeon, Hwang-Ju,Kim, Jun-Ran,Lee, Sung-Eun 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.3
본 연구는 식물검역 분야에서 주요하게 사용되고 있는 메틸브로마이드 훈증제로 인해 발생하는 약해를 저감하기 위한 물질을 모델식물인 애기장대를 이용하여 스크리닝하였다. 사전연구를 통하여 메틸브로마이드 훈증제의 식물 독성 메커니즘으로 활성산소발생와 식물 성장 호르몬인 옥신의 식물체 내 분배억제효과가 발생하는 것을 바탕으로 하여, 약해 저감물질후보군으로 활성산소를 제거하는 역할을 하는 ROS scavenger 2종(NAC, GSH)과 옥신을 훈증제 처리 전 애기장대에 처리한 후 약해의 저감 정도를 육안평가와 더불어 관련 유전자의 발현을 확인하였다. 연구 결과 메틸브로마이드에 의해 유도된 약해는 옥신보다는 활성산소를 저감시키는 물질후보군들에서 약해 저감효과가 나타났다. 이 중 GSH을 이용하여 농도구배하여 전처리하였을 때, 5 mM GSH 전처리 후 메틸브로마이드 훈증시 약해 저감효과가 두드러졌다. GSH 전처리 시 식물체 내에 MBF1c와 HSP70 유전자 발현이 증가하는 것을 확인하였으며, 이는 메틸브로마이드 훈증으로 유도되는 약해를 방어하는 역할을 담당하였을 것이라고 평가된다. 따라서, 식물검역 훈증제 메틸브로마이드에 의해 발생하는 약해를 저감하는 데 GSH의 사용가능성을 평가하였으며, 이를 기반으로 다양한 식물체에 적용하여 수출입 시 약해로 인한 경제적 손실을 감소시킬 수 있기를 기대한다. Understanding the phytotoxic mechanism of methyl bromide (MB), an essential fumigant during the quarantine and pre-shipment process, is urgently needed to ensure its proper use and reduce international economic losses. In a previous study, two main MB-induced toxic mechanisms such as reactive oxygen species (ROS) and auxin distribution were selected by analyzing transcriptomic analysis. In the study, a 3-week-old A. thaliana was supplied with 1 mM ROS scavengers [N-acetyl-L-cysteine (NAC) or L-glutathione (GSH)] and 1µM indole-3-acetic acid(IAA) three times every 12 h, and visual and gene expression assessments were performed to evaluate the reduction in phytotoxicity by supplements. Phytotoxic effects on the MB-4h exposed group were decreased with GSH application compared to the other single supplements and a combination of supplements at 7 days post fumigation. Among these supplements, GSH at a concentration of 1, 2, and 5mM was suppled to A. thaliana with MB-fumigation. During a long-term observation of 2 weeks after the fumigation, 5 mM GSH application was the most effective in minimizing MB-induced phytotoxic effects with up-regulation of HSP70 expression and increase in main stem length. These results indicated that ROS was a main key factor of MB-induced phytotoxicity and that GSH can be used as a supplement to reduce the phytotoxicity of MB.
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