Caffeic acid phenethyl ester의 처리에 의한 NSAID activated gene-1의 과대발현

장민정(Min-Jeong Jang),김효은(Hyo-Eun Kim),손성민(Seong-Min Son),김민정(Min-Jeong Kim),서을원(Eul-Won Seo),김영호(Young-Ho Kim),김종식(Jong-Sik Kim) 한국생명과학회 2009 생명과학회지 Vol.19 No.12

파이토케미칼의 일종인 CAPE가 암세포 생장에 미치는 영향과 유전자 발현을 연구하기 위하여, 인간 대장암 세포주 HCT116에 CAPE를 처리하였다. CAPE의 처리는 농도 의존적으로 암 세포 생존율을 감소시키고, 세포사멸을 유도함을 확인하였다. CAPE에 의해 차별적으로 발현되는 유전자를 분석하기 위하여, oligo DNA microarray 실험을 수행하였다. 그 결과, 20 μM CAPE를 24시간 동안 처리한 경우, 2배 이상 발현이 증가되는 유전자 266개, 2배 이상 발현이 감소되는 유전자 143개를 확인하였다. 발현이 증가되는 유전자중 3개(NAG-1, p21, GADD45A)를 선택하여, RT-PCR을 수행하였다. 그 결과, 모든 유전자의 발현이 마이크로어레이 실험결과와 일치하였다. 또한, CAPE를 농도 의존적으로 처리한 후, NAG-1 유전자와 단백질의 발현을 확인한 결과, mRNA 수준과 단백질 수준에서의 발현양상이 동일함을 확인하였다. 게다가, CAPE를 포함한 5개의 다른 종류의 파이토케미칼(resveratrol, genistein, daidzein, capsaicin)을 처리한 경우, 처리한 모든 파이토케미칼에 의해 NAG-1 유전자의 발현이 증가됨을 확인하였다. 이중 CAPE가 가장 낮은 농도의 처리임에도 불구하고 NAG-1의 발현을 가장 강하게 유도하였다. 결론적으로 이러한 연구결과는 CAPE에 의한 세포사멸은 항암유전자인 NAG-1의 과대발현과 밀접한 관련이 있음을 의미한다. To investigate whether caffeic acid phenethyl ester (CAPE) could affect cancer cell viabilities and gene expression, human colorectal HCT116 cells were incubated with CAPE. CAPE decreased cancer cell viabilities and induced apoptosis in a dose-dependent manner. To analyse differently expressed genes by CAPE, we performed oligo DNA microarray analysis. We found that 266 genes were up-regulated more than twofold, whereas 143 genes were down-regulated more than twofold by 24 hr of treatment with 20 μM CAPE. Among the up-regulated genes, we selected 3 genes (NSAID activated gene-1 [NAG-1], cyclin-dependent kinase inhibitor 1A [CDKN1A, p21] and growth arrest and DNA-damage-inducible alpha [GADD45A]) and performed reverse-transcription PCR to confirm microarray data. In addition, we found that CAPE increased NAG-1 gene and NAG-1 protein expression in a dose-dependent manner. And, several other phytochemicals (resveratrol, genistein, daidzein and capsaicin) also could induce NAG-1 expression in human colorectal HCT116 cells. However, CAPE was the highest inducer of NAG-1, even in low concentrations. Overall, these results imply that cancer cell death by CAPE is closely related with over-expression of NAG-1.

5α-환원효소 결핍증

장민정 ( Min Jeong Jang ),오수영 ( Su Young Oh ),최성은 ( Seong Eun Choi ),오훈규 ( Hoon Kyu Oh ),김덕윤 ( Duk Yoon Kim ),최윤석 ( Youn Seok Choi ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.3

5 alpha-reductase deficiency is a rare autosomal recessive disorder caused by mutations in the SRD5A2-gene, resulting in absent or diminished dihydrotestosterone (DHT) formation and, hence, in an underdevelopment of the external genitalia in patients with 46,XY karyotype. Recently we experienced a 17 years old patient with chief complaint of primary amenorrhea, who showed 46,XY karyotype, enlarged clitoris, virilization, undeveloped breast and palpable bilateral inguinal mass. We diagnosed it as 5 alpha-reductase deficiency and removed the bilateral gonads, so we report it with brief review of literature.

브랜드 이미지 강화를 위한 패밀리 룩(Family Look) 패키지디자인에 관한 연구 : 제과 업계의 빙과류 제품을 중심으로

장민정(Jang Min-Jeong) 한국디자인학회 2012 한국디자인학회 학술발표대회 논문집 Vol.2012 No.5

농산물 별 Matrix (색소) 제거 효과에 따른 분석 효율성 검토

장민정 ( Min-jung Chang ),김현빈 ( Hyun-bin Kim ),육경희 ( Kyung-hee Yuk ),김지혜 ( Ji-hye Kim ),이지환 ( Ji-hwan Lee ),권순덕 ( Soon-duk Kwon ),함홍주 ( Houng-ju Ham ),김기주 ( Ki-ju Kim ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

본 연구는 농약분석 시 시료(농산물) 종류에 따라 함유되어 있는 매질 등 방해 성분을 제거하여 정확한 분석 결과를 도출하고, 전처리 과정에서 매질과 분석 대상 성분들이 동시에 용출되어 분석 장비 효율성이 저하되는 문제점을 해소하고자 한다. 서로 다른 종류의 색소(클로로필, 카로티노이 드, 안토잔틴, 안토시아닌)를 함유한 쑥갓, 고춧가루, 양배추, 블루베리 4품목을 대상으로 matrix 제거 효과 연구를 진행하였다. 추출은 보편화 된 EN method로 하였으며, 정제 kit 로는 Basic (25 mg PSA, MgSO₄ 150 mg), C18 (25 mg PSA, 25 mg C18, MgSO₄ 150 mg), 2.5 mg GCB (25 mg PSA, 2.5 mg GCB, MgSO₄ 150 mg), 7.5 mg GCB (25 mg PSA, 7.5 mg GCB, MgSO₄ 150 mg) 4 가지 종류를 사용하여 QuEChERS (Quick Easy Cheap Effective Rugged Safe)방법으로 전처리를 진행하였다. GC-MS/MS 와 LC-MS/MS 분석 장비를 이용하여 다성분 동시 분석농약 320 종에 대해 조사하였고, 쑥갓, 고춧가루, 양배추, 블루베리 모두 7.5 mg GCB kit에서 육안상 가장 큰 정제 효과를 보였으나, 평면구조를 가진 농약 등에서 잔류분석기준(회수율 70~120 %)을 벗어나는 성분 이 증가하였다. GC-MS/MS 경우 Prochloraz (양배추, 블루베리) 성분이 7.5 mg GCB kit에서만 잔 류분석기준을 만족하였으며, 크로마토그램 확인 결과 쑥갓, 양배추, 블루베리는 7.5 mg GCB kit, 고춧가루는 C18 kit에서 깨끗한 base line을 확인하였다. LC-MS/MS 경우 Spirotetramat (쑥갓), Fenamiphos, Halosufuron-methyl, Molinate, Spirotetramat (블루베리), Metazosulfuron (양배추), Diflubenzuron, Etofenprox, Oxamyl (고춧가루) 성분이 실험에서 사용한 모든 정제 kit에서 회수율 이 상승하였다. 본 연구 결과를 활용하여 품목·성분별 가장 효율적인 정제 kit를 사용함으로써 농약 분석 결과의 정확도 향상 및 장비 유지 보수에 도움이 될 것으로 사료된다.

초현실주의 특징이 반영된 한국 복식 디자인 연구

장민정(Chang Min-Jung) 한복문화학회 2017 韓服文化 Vol.20 No.3

Surrealism is adopted in design as a way to create unique and distinct design deviated from ordinary things, and it serves as a means to quench contemporary persons’ thirst for novelty and individuality. Derived from surrealism, there emerged costume reflecting characteristics of surrealism, and progressive design costumes applying surrealism styles have continuously come to surfaces up to today. Such costumes resulted in diversification of expression deviated from traditional costume with formality, and they lay out unprecedented experimental and progressive design based on designer’s creativeness and imagination. Surrealism is well suited to articulating individuality in that it is differentiated from traditional artistic formality and offers to broaden the possibilities of free expression and creation, this research is focused on designing and producing Korean costume reflecting characteristics of surrealism. Among Korean costumes, “Po (a generic term referring to an outer robe)” was selected given that it involves many compositions and its long length allows various designs and styles. This also reflects on concept and developments of surrealism, and also includes analysis on the characteristics of surrealism to design and produce Korean costume reflecting it. The purpose of this research is to blend traditional Korean costume with Western artistic movement surrealism to offer broadened views on Korean costume design. In succession to Korean costume, this offers experimental and various designs in line with changing trends, and proposes a new concept meeting contemporary persons’ emotional needs and carves out Korean costume market, thereby setting the direction for Korean costume design and serving as a reference for creative research on Korean costume design down the road. These are where the values of this research lie in. Hopefully, this research could also lay a foundation for another attempt towards novel design work by mixing Korean costume with Western art trend other than surrealism which would lead to Korean costume design in line with changing trends and befitting contemporary persons’ individuality, thereby further lifting up Korean costume.

토크쇼 대화에 나타난 질문-응대의 전략 분석

장민정 ( Min Jung Jang ) 한국텍스트언어학회 2011 텍스트언어학 Vol.30 No.-

The aim of this paper is to address strategies for the adjacency pairs of ``question-answer`` in the TV talk-show, <People inside> which is very popular in Korea. First, we discuss a general theoretical frame. Second, we describe main characters in the show. There are two participants, one host and one guest in the talk show. The strategies of the semi-institutional conversation in one on one conversation has been found in this respect. As a result of analysis of the conversations in the talk show, there are three questions types which are namely confirming information, fact seeking and emotional expressions demand. According to the question types, various strategies for answer has also been found which has led to interlocutor`s roles and goals of the conversation.

다문화가정에 대한 이미지 읽기

장민정(Jang Min Jung) 한양대학교 수행인문학연구소 2011 수행인문학 Vol.41 No.1

In this study we focus on advertising and multiculturalism in Korea and look into the narrative aspects of Multicultural advertising in order to find how to make an image of Multicultural families in our society. The rapid growth of the Multicultural family dictates investigation of these study’s needs, therefore aimed to gain an in-depth understanding of factors related to the identification and placement of Multicultural family’s in Korean society. This study attempts to analyze narrative structures of advertisement on these issues using narrative theories of Greimas. Multicultural advertisements provide insight on current thinking about the influence of diverse populations in society. To situate the research stages of what is now called multicultural family, the first question is addressed by examining the early stage of research on Multiculturalism in society. This analysis involves revealing the signification process of narrative of multicultural advertisements. As a result, it produces some stereotypes of the immigrant Asian woman and her children. Despite the increasing number of multicultural families, multiculturalism has not yet fully heightened in Korea.

동양미학적 관점에서 본 한국 전통복식의 조형적 특성 연구

장민정(Chang Min-jung) 한복문화학회 2018 韓服文化 Vol.21 No.2

The purpose of this study is to establish the theoretical viewpoints of Oriental aesthetic viewpoints and analyze the formative characteristics of Korean traditional costumes based on them. The oriental aesthetic perspective is based on the characteristics of Oriental aesthetics in previous studies, and the heaven, nature, and human being are the root of oriental aesthetics. It is classified into shape, silhouette, material and color, pattern and decoration. The oriental aesthetic ideology is manifested in various forms according to the age and area, and not only the original aesthetic consciousness has developed, but also aesthetic reflection on it has been actively developed. In the course of this process, Oriental aesthetics has a common and continuous character and its aesthetic elements are revealed through oriental culture, thought and philosophy. At present, there are many studies on the philosophy and thought about oriental aesthetics, but the relationship between oriental aesthetics and Korean costume has not been studied yet. Therefore, the present researcher wants to improve the importance of traditional costume and the spirit of Oriental spirit by analyzing the characteristics of Korean costume based on the principle explanation of Oriental aesthetics at the present time when the harmony of East and Western aesthetics is required. In order to discuss the theoretical aspects of oriental aesthetic viewpoint, the research method is based on the literature related to costume, ideology, philosophy and aesthetics, and previous researches. Based on this research, this study tries to examine the formative characteristics of Korean traditional costumes from the aesthetic point of view. In order to develop Korean costumes in a global modern society, it is necessary to identify our own identity and formative characteristics and to approach Korean traditional costumes based on understanding of oriental aesthetics. This research will help to present a new direction to the aesthetic research of Korean traditional costume culture.

통합교육에 대한 한국과 중국 일반아동의 인식

장민정 ( Zhang Min Ting ),임봉숙 ( Lim Bong Suk ),정재권 ( Chung Jae Kweon ) 대구대학교 특수교육재활과학연구소 2014 특수교육재활과학연구 Vol.53 No.1

본 연구는 통합교육에 대한 한국과 중국 일반아동의 인식을 알아보고, 어떤 차이가 있는지를 분석하여 성공적인 통합교육의 기초자료를 제공하는데 그 목적이 있다. 구체적인 연구 목적으로서는 통합교육에 대한 한국과 중국 일반아동의 인식 차이를 밝히고자 하였다. 이를 위해 한국과 중국 각 국가별 200명으로 선정하였고, 연구자가 직접 작성한 일반아동용 설문지를 배포하여 그 중 회수된 한국 일반아동 175부와 중국 일반아동 180부의 설문지를 본 연구의 분석 대상으로 통계 처리하였다. 자료처리는 양국 인식비교에서 독립표본 t 검증을 실시하였다. 연구 결과를 통해 얻어진 결론은 다음과 같다. 첫째, 전체적으로 통합교육에 대한 한국과 중국 일반아동들의 전체 인식에 차이가 나타났으며, 통합교육에 대한 심리적 인식, 학습적 인식, 생활적 인식에서 모두 중국 일반 아동들이 한국 일반아동들보다 통합교육에 대하여 높게 인식하는 것으로 나타났다. 둘째, 통합교육에 대한 한국과 중국 일반아동의 변인에 따른 인식은 다양하게 나타났다. 전체적으로 볼 때 중국 일반아동들이 한국 일반아동들보다 통합교육 대하여 더 긍정적인 인식을 가지고 있다. The purpose of this study was to provide the basic data for successful integrated education by looking into the perception towards the integrated education among ordinary children of Korea and China and analyzing the difference in such perception. Specifically, the objective of this study was to determine the difference in perception towards the integrated education among the ordinary children of Korea and China. For that, 200 subjects, each from Korea and China, were selected for this study, and the survey sheets designed for ordinary children, which had been produced by this researcher, were distributed. 175 questionnaire sheets were collected from Korean ordinary children and 180 questionnaire sheets were collected from Chinese ordinary children. The collected questionnaires were analyzed statistically. For the processing fo data, the independent sample t-test was conducted to analyze the perception among the ordinary children of each country. Based on the results of this study, the following conclusion was drawn: First, a difference was found in overall perception towards the integrated education among the ordinary children from Korea and China. In relation to the psychological perception, learning perception, and everyday life perception with respect to the integrated education, the ordinary children of China was found to have higher perception toward the integrated education than the ordinary children of Korea. Second, the diversity was found to exist in the perception of ordinary children of Korea and China towards the integrated education based on the variables. In overall way, ordinary children of China had more positive perception towards the integrated education than the ordinary children of Korea.

전통 조각보를 응용한 패션문화상품 개발 (1) : 앞치마를 중심으로

장민정(Chang Min-Jung) 한복문화학회 2010 韓服文化 Vol.13 No.3

In this study, we try to find Korea’s traditional beauty and design elements by providing the identity of Korea for development and new challenges of Korea’s fashion industry. By reinterpret the culture products as modernistic, add avery Korean twist to a worldwide fashionable products form. As it is a great traditional works of art form which contains configuration of the refinement, the combination of colors, and also maker’s formative works of art, we need to put Korea's patchwork for design motif by applying to an apron within colors, materials, and textile etc. through out the process, our traditional beauty will be sufficient enough to revalue in the process of globalization and also be able to get a chance to let people know about our culture in worldwide by making and designing in public so that they could get closed to our traditional culture. Aprons, which can be used in various parts and functions, are very accessible item to people of all ages and have great uses. So if we seek it as a high value product then it will surely contribute to fashion industry but also let world know about our culture.