한국산 충생자낭균류의 미기록종에 관하여

이용보,임채규 ( Yong Bo Lee,Chae Kyu Lim ) 한국균학회 1998 韓國菌學會誌 Vol.26 No.3

한국에서 채집된 Laboulbenia 균류에 관하여 - 제주지역을 중심으로 -

이용보,임채규,나영희 ( Yong Bo Lee,Chae Kyu Lim,Young Hee Na ) 한국균학회 1995 韓國菌學會誌 Vol.23 No.1

한국에서 채집된 충생자낭균류에 관하여 ( 경기도 광릉지역을 중심으로 )

이용보,곽칠선,임채규,나영희 ( Yong Bo Lee,Chil Sun Gwak,Chae Kyu Lim,Young Hee Na ) 한국균학회 1996 韓國菌學會誌 Vol.24 No.3

단보 : 한국산 충생자낭균류의 미기록종에 관하여(2011)

이용보 ( Yong Bo Lee ),임채규 ( Chae Kyu Lim ),나영희 ( Young Hee Na ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.2

Five species of the Laboulbeniales are reported to the Korean mycoflora. They are Laboulbenia acupalpi Spegazzini on Acupalpus hilaris Tschitscherine, L. pseudomasei Thaxter on Anoplogenius cyanescens(Hope), Rickia peyerimhoffii Maire on Scaphidium femorale Lewis, Zodiomyces odae Majewski et Sugiyama on Sternolophus rufipes Fabr. and Z. vorticellarius Thaxter on Berosus fairmairei Zaitzev. Among these species L. pseudomasei Thaxter and Z. odae Majewski et Sugiyama are the first report to South Korea. L. acupalpi Spegazzini and Z. vorticellarius Thaxter found on species which are different from previous to host insects. R. peyerimhoffii Maire is newly described main land of Korea, although it was reported from Jeju island.

낙동벼(Oryza sativa L.)로부터 유도된 엽록소 돌연변이체의 특성

김홍섭(Hong-Sub Kim),임채규(Chae-Kyu Lim),고재문(Jae-Moon Koh),김종세(Jong-Se Kim),이숙영(Sook-Young Lee) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.1

인위 돌연변이 유기에 의해 벼로 부터 새로운 유전형질체를 개발하기 위하여 낙동벼(모품종)에서 고정된 엽록소 결핍계통(ch mutant)을 선발하였다. 이 계통은 생육초기 부터 엽록소 결핍으로 정상엽에 비하여 노란색을 나타내기 때문에 엽록소의 광합성 관여 유전자탐색에 이용가능하다. ch mutant는 전 생육기간 동안 모품종의 51~87% 정도의 엽록소 함량을 보였으나 엽록소 a/b 함량비는 차이가 없었다. ch mutant의 총 엽록소 함량은 모품종의 70.2%였으며 anthocyanin 함량과 flavonol 함량은 각각 285%와 142%로 높은 함량비를 나타냈다. ch mutant의 카로티노이드 함량은 모 품종의 71.1%였고, 크산토필 함량은 56.6%였다. 특히 카로티노이드성분 중 lutein과 neoaxanthin 함량은 각각 모품종의 32%와 34.4%로 매우 낮았으며, β-카로틴은 차이가 없으나 antheraxznthin 함량은 106.9% 오히려 증가하였다. 개엽상태에서나 군락상태에서 ch mutant의 반사율과 투과율은 모두 모품종에 비하여 높아 광흡수량은 저하되었다. ch mutant 는 모품종에 비하여 출수기가 5일 늦고 간장, 수장, 주당수수, 수당 엽수, 임실율, 천립중 및 수량에서 감소하였다. chmutant와 모품종은 esterase, phosphoglucose isomerase, malic enzyme, hexokinase 동위효소의 밴드패턴에서 유의할만한 차이를 나타내지 않았으나 엽록체 단백질의 경우 주요밴드인 60KD의 분자량에서 차이를 보였다. Chlorina(ch) mutant, the chlorophyll-deficiency strain was induced from the original rice called Nagdongbyeo(Oryza sativa L.). The present study have been undertaken to characterize chlorina mutant by comparing with the original relating to physiological and biochemical properties. The chlorina mutant would be useful as a strain to detect genes involved with chlorophyll deficiency because of yellow color development in early stage of growth. a/b content ratio of chlorophyll in the ch mutant was identical with the original in all growth period but total chlorophyll contents were only 70.2% of the original. Anthocyanin and flavonol of ch mutant were remarkably increased to 285 and 142% respectively. Also the contents of carotenoids and xanthophylls in ch mutant were given 71.7 and 56.5% of the original respectively. In case of carotenoid lutein and neoxanthin decreased to 32 and 34.4%, respectively but antheranthins increased to 106.7%. β-Carotene content was the same as the original. In the individual or community condition, the reflexibility and transmission was increased in ch mutant than those in original but absortivity decreased. The earing season of ch mutant was five days late as compared to origianl ; in addition, culm and panicle length, number of panicle per hill and spikelets per panicle ripened grain ratio, 1,000-grain weight and yield decreased. Any significant difference was not observed in the pattern of isozyme such as esterase, phosphoglucose isomerase, malic enzyme and hexokinase. But the total protein pattern of chloroplast was remarkably different from each other.

갈대 및 가는갯능쟁이의 발아에 미치는 NaCl 의 효과

김관수(Kwan Soo Kim),문용식(Yong Sick Moon),임채규(Chae Kyu Lim) 한국식물학회 1985 Journal of Plant Biology Vol.28 No.3

The results of the conducted experiment obtained basic data on seed germination for Phragmites communis and Atriplex gmelini were; Seed germination was not influenced till 0.5% NaCl, but at over 1.0% NaCl it dropped remarkably. The germination limit for degree of NaCl was 2.0% in Phragmites communis. Atriplex gemelini was 2.5% and in accordance with the increase of the degree of NaCl. Germination speed showed a negative correlation being highly significant and the germination period lengthened. Compared with growing top plants, growth of roots was largely influenced by a high degree of NaCl. In accordance with the rise of temparature, the germination rate, and speed of both plants remarkably increased and the germination period was shortened. Ubride of Atriplex gmelini was germinated at the early days of picking but was not as the passing of the period. The seeds also did not germinated likewise Ubride. By a seedcoat breaking germination became 81%. During 20 min soaking treatment in conc H_2SO_4 seed germination possibility of 63% was known to be hard. Adequate soaking time in conc H_2SO_4 was 17.5 min.

속성 퇴비화에 관한 연구

임채규,박경수,김홍섭,노영복 조선대학교 기초과학연구소 1988 自然科學硏究 Vol.11 No.1

This experiment was conducted to fine out the rapid composting method and the effects microorganism agent. The results were summarilized as follows; 1. Each temperatures of every composts highest rised at the 5 days during the experiment composting. 2. Composting temperature of the more 60℃ was killed the harmful lavar and pathogenic microorganisms. 3. The lowest C/N ratio was No.2, among the each treatments. 4. C/N ratio of the each treatments were same results of the general compost C/N ratio. 5. The synthesized of these results were recognized the effects of microorganism agent and the each treatments were showed the more rapid composting then general compost.

퇴비 부숙제에 관한 연구

임채규,박경수,노영복 조선대학교 농업연구소 1988 農業硏究 Vol.1988 No.1

1. 셀로스타트 제재의 미생물은 작물의 발아율에 영향을 미치지 않는 것으로 나타났다. 2. 미생물의 수는 세균이 4×10?, 방선균이 3.2×10?, 곰팡이가 3.5×10³으로 나타났다. 그들의 비는 세균/방성균이 320.3이고, 세균/곰팡이 비는 29,285.7로 나타났다. 3. 퇴비의 원료가 대부분 섬유소이기 때문에 섬유소 분해 능력이 우수한 균주를 사용한 것 같으며 분리 균주의 섬유소 분해력은 세균보다 방선균, 곰팡이 쪽이 강하게 나타났다. 4. 셀로스타트 제재의 미생물 동정은 다음과 같다. Bacillus circulans, Bacillus sphaericus, Bacillus sp., Bacillus stearothemophilus, Bacillus coagulans, Bacillus fastidiosus, Bacillus amylolicuefaciens, Streptomycetes Griseoauranticus, Streptomycetes nigrifaciens, Streptomycetes spl., Mucor sp., Penicilliumjaponicus, Aspergillus sp.였다. Rural compost was prepared from organic residues such as crop residues, cattle dung and other animal wastes. The organic substances undergo intensive decomposition under thermophilic and mesophilic conditions in heaps or pits with adequate moisture. Finally compost yield a dark coloured humified material in three to six months. Bioconversion of organic matter was carried out by different groups of heterotrophic microorganisms, that is bacteria, actinomycetes and fungi. Esentially composting was an orderly sequence of harmonious biological functions resulting in the reduction of organic agricultural waste. According to the Cellostat reports, the result of application of Cellostat in any case was a faster and more complete decomposition of organic substances into highly valuable fertilizing compose. More over, Cellos tat treated compost improves the soil quality, increases the growth in plant. So, this experiment was conducted for isolation and identification of the microorganism in Cellos tat agent. The results Were summarilized as follows; 1. Microorganism or Cellostat agent was not showed the effect for seed germination of rice, tomato and spianch. 2. Microflora was showed bacteria 4 × 10^6, actinomycetes 3.2 × 10^5 and fungi 3.5 × 10^3. They ratior was B/A 320.3 and B/F 29285.7 respectively. 3. Particulary, microorganism of Cellostat agent would be used superior strains of cellulose fermentation capacity, because compost: material was most cellulose. 4. As a result, each species were identified as follows; Bacillus circulans, B. sphaericus, B. stearothermophilus B. coagulans. B. fastidiosus, B. amylolicuefaciells, B. sp., Streptomycetes griseoauranticus, S. nigrifaciens, S. sp., Muco sp., Penicillium japonicus, Aspergillus sp..

寶城郡의 綠地保全地域 指定을 爲한 基礎調査

李龍保,任采圭 조선대학교 부설 유전생물공학연구소 1993 유전생물공학연구지 Vol.3 No.-

This survey area was the Bousng country located in the south end of the Johnnam province. The survey object was high Mt. grassland, primitive forest and the prime forest (up to 20~50 years old) near the natural vegetation. It was up to the 8 degrees of the Green naturality of the first national survey to the natural ecosystem. With the preservation law of the natural environment established (September,1992), this field survey was carried out to designate and control this area as the Green Preservation Area during 6 months from May to October, 1992. The results were as follows; 1. In the area of the survey object, the area of the green preservation area was 42,538㎢. The area up to the 8 degrees by the present mesh and the area of this precise survey were 47㎢ and 42.172㎢, respectively. 2. The dry matter biomass, productivity and O_2 productivity were 297,766t/y, 122,970.3t/y and 24,348.8t/y respectively. 3. By the land use division according to the administration law of the land utilization, the total survey area is 662.444㎢, the area for the forest preservation 403.569㎢(60.9%), the preservation area of natural environment 2.350㎢, the farmland, the urban land, orderly. 4. In the vegetation of survey area, the dormancy species was chiefly the oak tree (Quercus serrata, Quercus aliena, Quercus mongalica, Quercus accutissima, Quercus dentata, Quercus vuriabilis) and the complex forest was made up of Capinus laxiflora, Salix koreensis, Fraxinus mardshurica, Symplocos chimersis, Sasa borealis, Lespedeza bicolor. In the plant community of this area investigated, Quercus serrata community was 0.699㎢, Quercus acutissima community 2.852㎢ and Quercus mongolica – Quercus variabili community 3.497㎢. 5. The vascular plants around Mt. chunbong and malbong are 808 kinds belonging to 117 families, 401 genera, 698 species 103 varieties and 7 forms. The special plants were Lindera sericea and Corylopsis coreana. Which didn’t live naterally in Mt. moodeung, lived at the nearest area to Mt. moodeung. 6. A pasture of the cattle and goat within the green preservation should ban. And then, we think that it should set up the control plan of the green preservation area to the absent owner of mountain.

水稻에 있어서 葉綠素 突然變異系統의 生理·生化學的 特性에 관한 硏究

金弘燮,林采圭 조선대학교 기초과학연구소 1993 自然科學硏究 Vol.16 No.1

인위 돌연변이 유기에 의한 새로운 유전형질의 개발과 유용한 유전자 정보를 밝히기 위하여 낙동벼에서 유기된 엽록소 돌연변이 계통에 대하여 생리 생화학적 특성을 조사하였다. 얻어진 결과를 요약하면 다음과 같다. 1. 낙동벼에서 고정된 엽록소 결핍계통을 선발하였다. 이 계통은 생육초기부터 엽록소결핍으로 정상엽에 비하여 노란색을 나타내기 때문에 엽록소의 광합 성 관여 유전자 탐색에 이 용가능하다. 2. 엽록소결핍계통은 전 생육기간동안 낙동벼의 51~87% 정도의 엽록소 함량을 보였으나 엽록소 a/b함량비는 차이가 없었다. 엽 록소 결핍계통의 총 엽록소 함량은 낙동벼의 70.2%였으며 anthocy-anin함량과 flavonol함량은 각각 285% 은 142% 로 높 은 함량비를 나타냈다. 3.엽록소 결핍계통의 carotenoids함량은 낙동벼의 71.1%였고, xanthophylls함량은 56.5%였다. 특히 carotenoids성분중 lutein과 neoaxanthin함량의 감소 가 커서 각각 낙동벼의 32%와 34.4%로 매우 낮았으며, β-carotene은 차이가 없었고 antheraxanthin함량은 오히 려 증가하여 106.9%를 나타냈다. 4. 개엽상태에서나 군락상태에서 엽록소 결핍계통의 반사율과 투과율은 모두 낙동벼에 비하 여 많아 광흡수량은 떨어졌다. 5. 엽록소 결핍계통은 낙동벼에 비하여 출수기가 5일 늦고 간장, 수장, 주당수수, 수당입수, 임실율, 천립중 및 수량에서 감소하였다. 6. 엽록소 결핍계통과 낙동벼는 esterase, phosphog1ucose isomerase, malic enzyme, hexokinase 동위원소 특성에서 차이를 나타내지 않았다. 그러나 잎 에서 엽록체만을 순수분리 하여 추출한 엽록체 단백질 pattern에서는 차이를 보였다. Morphological, physiological and biochemical characteristics were examined in the mutant strains of chlorophyll induced in rice(Oryza sativa L).It was to develop a new inherited character and manifest a useful information of genes by induction of its artificial mutations. The results were summarized as follows : 1. The chlorophyll-deficiency strains fixed in the rice (Oryza sativa L.) were selected. This strains will be very of useful as a material to detect genes connected with phot-os3'llthesis of chlorophyll because of displaying yellow colors by chlorophyll-deficiency of leaver compared with normal leaves from early growth. 2. The chlorophyll-deficiency strains didn't differ between a/b content ratio of chlorophyll. Where as, 1 was shown that between but between about 51 and 87 percent contents of chlorophyll compared with the rice during all growing period. Total chlorophyll contents of these strains were measured as only 70.2% of the rice(Oryza sativa L.), but the contents of antocyanin and flavonol were highly increased to 285 and 142 percent respectively. 3. In the chlorophyll-deficiency strains compared with the rice(Oryza sativa L.), the contents of carotenoids and xanthophylls were 71 1 and 56.5 percent repectively. Especially in the carotenoid ingredients, the contents of lutein and neoxanthin highly decreased to 32 and 34.4 persent respectively, but the contents of β-carotene were equal and the contents of antheraxanthins increased to 106.7 percent. 4. In the individual or community condition, the reflexiblity and transmission of the chlorophylldefieiency strains increased and its absorptivity decreased in comparision with the rice(Oryza sativa L. ) 5. In the chlorophyll-deficiency strains compared with the rice(0ryza sativa L.), the earing season was fivedays late, culm length, panicle length, number of panicle perhill, number of spikelets per panicle, ripened grain ratio and 1000-grain weight and vield decreased. 6. The chloroph11-deficiency strains and rice(0ryza sativa L.) differed little in the characteristics of isoenzymes, esterase, phosphogluicose, isomerase, malic enzyme and hexokinase. But the protein pattern of chloroplast extracted by pure seperation differed.