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면역점착 적혈구 응집반응에 의한 한탄바이러스 및 서울바이러스 감염의 감별 및 홍콩 집쥐에서 분리한 바이러스의 혈청형 분류
이평우,안인숙,이호왕,W . K . Chang 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.2
Immune adherence hemagglutination (IAHA) test was used for serotypic classification of Hong Kong virus which was isolated from lungs of rat captured in Hong Kong previously and for differentiation of Hantaan viral infection frorn Seoul viral infection in this study. The optimal condition of sonication for IAHA antigen preparstion and the peak of expression of antigen in infected cells were determined for this study. The obtained results are as follow . 1. The titers of IAHA antigen prepared by sonication from 30 seconds up to 180 seconds at every 30 second intervals were same in all, 1: 256. 2. IAHA antigen in cells infected with Hantaan virus 76-118/RA strain was appeared at one day after inoculation of virus and then was reached at peak one week later and was maintained the same titer until 14 days and decreased 18 days after. 3. Hantaan virus infection could be differentiated from Seoul virus infection by cross-IAHA test. That is, the rat immune sera against 14 strains of Hantaan virus were 2-4 folds higher in reactivity with Hantaan virus than Seoul virus antigen. However the rat immune sera against 12 strains of Seoul virus were 2-128 folds higher in reactivity with homologous virus antigen. 4. The serotypic classification of a virus isolated from Hong Kong house rat: The cross- IAHA checkerboard titration of 4 strains of Hantaviruses, HTNV/76-118, SEOV/80-39, PUUV/Sotkarno and Hong Kong virus, with rat antisera against those viruses indicated that Hong Kong virus was more closer to Seoul virus than Hantaan or Puumala viruses.