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      • KCI등재

        잉어(Cyprinus carpio)로부터 분리된 Aeromonas hydrophila의 extracelluar proteases 연구

        이종규,김종필,최태진,송영환,Lee, Jong-Kyu,Kim, Jong-Pil,Choi, Tae-Jin,Song, Young-Hwan 한국어병학회 1997 한국어병학회지 Vol.10 No.1

        Aeromonas hydrophila isolated from the intestine of carp produced several kinds of proteases into the medium. Inhibitor assay with the culture supernatant of A. hydrophila showed that there were major metalloproteases and minor serine proteases. Gelatin SDS-PAGE showed two proteolytic bands. One broad protease band was inhibited by metalloprotease specific inhibitor, EDTA, indicating a metalloprotease. The other was inhibited by serine protease specific inhibitor, PMSF, suggesting a serine protease. The proteolytic activities of both extracellular proteases remained on Gelatin SDS-PAGE after heating at $70^{\circ}C$ for 30 min. However, the major metalloprotease was separated into two proteolytic bands on Gelatin PAGE by gel filtration chromatography on Sephadex G-75. 잉어로부터 분리한 Aeromonas hydrophila는 세포 밖으로 여러 종류의 proteases를 생산한다. A. hydrophila의 배양 상층액을 이용한 inhibitor assay를 통하여, 주된 활성을 나타내는 metallopretease와 약한 활성을 나타내는 serine protease가 있음을 알게 되었다. Gelatin SDS-PAGE를 통하여 두 개의 활성 band가 관찰되었으며, 이 들 중 넓게 퍼진 band는 metalloprotease에 특이하게 작용하는 inhibitor인 EDTA에 의해 활성이 상실되었고 따라서 metalloprotease임을 알 수 있었다. 다른 하나는 serine protease에 특이하게 반응하는 inhibitor인 PMSF에 의해 저해되어 serine protease임을 알 수 있었다. 이러한 두 extracellular protease의 활성은 $75^{\circ}C$에서 30 분간 열을 가한 후에도 Gelatin SDS-PAGE상에 남아있었다. 그런데, 주된 metalloprotease는 Sephadex G-75를 이용한 column chromatography를 거친 후 Gelatin Gel상에서 두 개의 band로 분리되었다.

      • SCOPUSKCI등재

        부분상태 궤환제어를 이용한 비선형 천정크레인의 진자각제어

        이종규,이상룡,Lee, Jong-Kyu,Lee, Sang-Ryong 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.6

        The purpose of this study is to design an anti-sway motion for industrial overhead cranes which transport objects on a horizontal plane by adjusting movements of a trolley motor and a girder motor. The movement of a hoist motor has not been considered at this time since its role was assumed to move objects only vertically, therefore, not to affect the swing motion of objects. The dynamic behavior of the swing motion shows nonlinear characteristics, which makes the design of anti-sway motion controller difficult. First of all, the nonlinear state equation for the motion of industrial overhead cranes has been derived. Then they have been linearized about normal operating states determined by the dynamic characteristics of motor motion-acceleration, constant speed, and deceleration, and deceleration, during transportation. The partial state feedback control algorithm based on this linearized state equation has been developed on order to suppress the swing motion. The simulation results have demonstrated satisfactory performance of the proposed controller.

      • SCOPUSKCI등재

        MgCO<sub>3</sub>와 사문석을 사용한 마그네시아 시멘트의 무기 첨가제 영향

        이종규,소정섭,Lee, Jong-Kyu,Soh, Jung-Sub 한국재료학회 2015 한국재료학회지 Vol.25 No.2

        The carbon dioxide($CO_2$) released while producing building materials is substantial and has been targeted as a leading contributor to global climate change. One of the most typical method to reducing $CO_2$ for building materials is the addition of slag and fly ash, like pozzolan material, while another method is reducing $CO_2$ production by carbon negative cement development. The MgO-based cement was from the low-temperature calcination of magnesite required less energy and emitted less $CO_2$ than the manufacturing of Portland cements. It is also believed that adding reactive MgO to Portland-pozzolan cements could improve their performance and also increase their capacity to absorb atmospheric $CO_2$. In this study, the basic research for magnesia cement using $MgCO_3$ and magnesium silicate ore (serpentine) as main starting materials, as well as silica fume, fly ash and blast furnace slag for the mineral admixture, were carried out for industrial waste material recycling. In order to increase the hydration activity, $MgCl_2$ was also added. To improve hydration activity, $MgCO_3$ and serpentinite were fired at $700^{\circ}C$ and autoclave treatment was conducted. In the case of $MgCO_3$ as starting material, hydration activity was the highest at firing temperature of $700^{\circ}C$. This $MgCO_3$ was completely transferred to MgO after firing. This occurred after the hydration reaction with water MgO was transferred completely to $Mg(OH)_2$ as a hydration product. In the case of using only $MgCO_3$, the compressive strength was 3.5MPa at 28 days. The addition of silica fume enhanced compressive strength to 5.5 MPa. In the composition of $MgCO_3$-serpentine, the addition of pozzolanic materials such as silica fume increased the compression strength. In particular, the addition of $MgCl_2$ compressive strength was increased to 80 MPa.

      • KCI등재

        무기질 충진재와 폴리우레탄을 활용한 유·무기 복합 단열소재의 특성 평가

        이종규,소정섭,노현경,Lee, Jong-Kyu,Soh, Jung-Sub,Noh, Hyun-Kyung 한국재료학회 2012 한국재료학회지 Vol.22 No.11

        Recently, inorganic-organic hybrid materials have attracted much attention not only for their excellent thermal conductivity but also for their flame retardant properties. In this study, the properties of organic-inorganic hybrid insulating materials using inorganic fillers and polyurethane foam with different foaming conditions have been investigated. The addition of 1.5 wt% water to polyurethane as foaming agent shows the best foaming properties. The pore size was decreased in the foaming body with increasing of the $CaCO_3$ addition. The apparent density and thermal conductivity were increased by increasing the $CaCO_3$ addition. With an increasing amount of $CaCO_3$ powder, the flame retardant property is improved, but the properties of thermal conductivity and apparent density tend to decrease. When the addition of fine particles of $CaCO_3$, the apparent density and thermal conductivity were increased and, also, with the addition of coarse particles over $45{\mu}m$ in size, the apparent density and thermal conductivity were increased as well. In this study, the adding of $CaCO_3$ with average particle size of $27{\mu}m$ led to the lowest thermal conductivity and apparent density. After evaluation with different inorganic fillers, $Mg(OH)_2$ showed the highest thermal conductivity; on the other hand, $CaCO_3$ showed the lowest thermal conductivity.

      • SCOPUSKCI등재

        MgCO<sub>3</sub>와 사문석을 사용한 마그네시아 시멘트의 특성평가

        이종규,소정섭,추용식,송훈,박지선,Lee, Jong-Kyu,Soh, Jung-Sub,Chu, Yong-Sik,Song, Hun,Park, Ji-Sun 한국재료학회 2012 한국재료학회지 Vol.22 No.11

        MgO based cement for the low-temperature calcination of magnesite required less energy and emitted less $CO_2$ than the manufacturing of Portland cements. Furthermore, adding reactive MgO to Portland-pozzolan cement can improve their performance and also increase their capacity to absorb atmospheric $CO_2$. In this study, the basic research for magnesia cement using $MgCO_3$ and magnesium silicate ore (serpentine) as starting materials was carried out. In order to increase the hydration activity, $MgCO_3$ and serpentinite were fired at a temperature higher than $600^{\circ}C$. In the case of $MgCO_3$ as starting material, hydration activity was highest at $700^{\circ}C$ firing temperature; this $MgCO_3$ was completely transformed to MgO after firing. After the hydration reaction with water, MgO was totally transformed to $Mg(OH)_2$ as hydration product. In the case of using only $MgCO_3$, compressive strength was 35 $kgf/cm^2$ after 28 days. The addition of silica fume and $Mg(OH)_2$ led to an enhancements of the compressive strength to 55 $kgf/cm^2$ and 50 $kgf/cm^2$, respectively. Serpentine led to an up to 20% increase in the compressive strength; however, addition of this material beyond 20% led to a decrease of the compressive strength. When we added $MgCl_2$, the compressive strength tends to increase.

      • SCOPUSKCI등재

        저온 소결 유전체에 관한 연구

        이종규,김왕섭,김경용,Lee, Jong-Kyu,Kim, Wang-Sup,Kim, Kyeong-Yong 한국재료학회 1992 한국재료학회지 Vol.2 No.4

        본 실험에서는 낮은 음의 온도계수를 갖는 저온 소결 유전체에 대해 연구하였다. 새로 개발된 재료의 조성은 Ti$O_2$(100-X) CuOx(X=1~5wt%)에 미량의 Mn$O_2$를 첨가 하였다. CuO를 첨가하지 않은 경우에는 저온 (90$0^{\circ}C$) 에서 소결이 진행되지 않았다. CuO 함량이 증가할수록 저온에서 소결이 가능하였으나, 유전율이 낮아지고 유전손실은 증가 하였다. Mn$O_2$를 0.6wt% 첨가한 경우 유전율과 Q값이 가장 높게 나타났다. Low-firing dielectric materials with negative small temperature coefficients were investigated. The newly developed materials are based on Ti$O_2$(100-x)/ CuOx(X=1~5wt%) with small amount of Mn$O_2$ additive. The sample without CuO was not sintered at 90$0^{\circ}C$. As CuO content was increased the sample could be sintered at low temperature. However, the dielectric constant was decreased and the dielectric loss was increased. In the case of adding 3wt% CuO and 0.6wt% Mn$O_2$, the dielectric constant and the Q values appeared very high.

      • KCI등재
      • KCI등재

        Phyllactinia guttata ( Wallr . ex Fr . ) Lev 에 의한 포플러흰가루병

        이종규,김경희,이창근 ( Chong Kyu Lee,Kyeong Hee Kim,Chang Keun Yi ) 한국산림과학회 1982 한국산림과학회지 Vol.58 No.1

        Powdery mildew has been observed on the several clones of Populus euramericana, P. deltoides, P. deltoides x P. maximowiczii in the nurseries of Chuncheongan-do and Kangwoen-do forest Research Institute in mid-October 1982. Powdery mildew fungus of Populus spp. was identified as phyllactinia guttata(Waller. ex Fr.) Lev which was reported as the powdery mildew fungus of Populus tomentosa Carr. Powdery mildew of Populus spp. caused by this fungus has not previously been reported in Korea. When we surveyed the occurrence of powdery mildew disease the leaves of P. deltoides R-89 were severely attacked by this fungus

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