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- 저자 : 이용혁(Young Hyuk Lee) (검색결과 4 건)
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난소 절제술을 실시한 암컵 쥐에서 석류 단일과 석류 복합 시료의 갱년기 증상 개선 효과
이용혁(Yong Hyuk Lee),현선희(Sun Hee Hyun),정세영(Se Young Choung) 대한약학회 2006 약학회지 Vol.50 No.3
Women's aging made a functional decline at ovary. This functional problem causes estrogen deficiency and many disorders, such as, sexual dysfunction, obesity, osteoporosis, urinary incontinence, skin and all body's aging, etc. We determined the ameliorative effects of some herbal extracts mixture on postmenopausal symptoms in ovariectomized Sprague Dawley rats. Nine-weeks-old female rats were ovariectomized and treated with singled pomegranate extracts (0.40, 1.20 g/kg/day) and mixed pomegranate extracts (1.08, 3.24 g/kg/day) for 8 weeks. Oral administration of pomegranate extracts for 8 weeks were increased serum estradiol level and improved the serum osteocalcin, ALP activity and bone spe- cific ALP level. And pomegranate extracts were reduced body weight, adipose tissue. Uterus weight in treated group tended to improve. In addition, the concentration of triglyceride and total cholesterol were lower after 8 weeks. HDL-cholesterol levels improved with does-dependency in treated groups. Collagen level on skin and cartilage showed a slight increase at treatment for 8 weeks. ∞These results showed that both of singular extract treatments and extracts mixture treatments were effective on general postmenopausal symptoms at overiectomized rats, and especially, the mixture treatments were more effective than singular treatment. ∞In conclusion, these herbal extracts' mixture showed improvement effect in general factors related with postmenopausal disorders.
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만성적인 에탄올 섭취 시 수종 천연 추출물 투여가 흰쥐의 혈청과 간의 지질 함량에 미치는 영향
김목경(Mok Kyung Kim),이용혁(Young Hyuk Lee),현선희(Sun Hee Hyun),정세영(Se Young Chung) 대한약학회 2005 약학회지 Vol.49 No.6
This study had been done for the investigation of the effect of Vitis vinifera extract (VV), Schisandra chinensis extract (SC), Taraxacum officinale extract (TO), Gardenia jasminoides extract (GJ), Angelica acutiloba extract (AA) and Paeonia japonica extract (PJ) on fatty liver and hepatotoxicity which was induced by Lieber-DeCarli ethanol liquid diet. Male Sprague-Dawley rats were randomly divided into eight groups: ethanol diet(ED), normal diet(ND), ED+VV(100㎎/㎏), ED+SC, ED+TO, ED+GJ, ED+AA, and ED+PJ (300㎎/㎏/day). Rats fed liquid diets for 6 weeks showed remarkable increase in serum and hepatic lipids indicating the onset of alcoholic fatty liver. The increasing levels of GPT, ALP activities in serum were observed in the groups fed with alcohol-containing diets compared to those of the ND group. The VV, SC, TO, GJ, AA and PJ groups were decreased the levels of triglyceride, free fatty acid and total cholesterol in serum and liver and GPT, ALP activities in serum. Therefore, they can be utilized as a health functional food or new drug candidate for fatty liver and hepatotoxicity which was induced by chronic alcohol consumption.
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김지형(Ji-Hyung Kim),이용혁(Yong-Hyuk Lee),권용성(Yong-Seung Kwon),염근영(Geun-Young Yeom),송종한(Jong-Han Song) 한국진공학회(ASCT) 1997 Applied Science and Convergence Technology Vol.6 No.2
본 연구에서는 1000 Å 두께의 Molybdenum 화합물 (Mo, Mo-N, MoSi₂, Mo-Si-N)의 Cu에 대한 확산방지막으로서의 특성을 면저항측정장비 (four-point-probe), XRD, XPS, SEM, RBS 분석을 통하여 조사하였다. 각 박막층은 de magnetron sputtering 장비를 이용하여 증착되었고 300℃-800℃의 온도구간에서 30분동안 진공열처리하였다. Mo 및 MoSi₂ 방지막은 낮은 온도에서 확산방지막으로서의 특성파괴를 보였다. 결정립계를 통한 Cu의 확산과 Mo-실리사이드내의 Si의 Cu와의 반응이 그 원인인 것으로 사료된다. 질소를 첨가한 시편의 경우 확산방지특성 파괴온도는 Mo-N방지막의 경우 650℃-30분, Mo-Si-N 방지막의 경우 700℃-30분으로 향상되었다. Cu와 Si의 확산은 방지막의 결정립계를 통하여 더욱 빠르게 확산된다. 따라서 증착시 결정립계를 질소와 같은 물질로 채워 Cu와 Si의 확산을 저지할 수 있을 것으로 사료된다. 본 실험결과에서의 질소첨가는 이와 같은 stuffing 효과외에도 Mo-실리사이드 박막의 결정화 온도를 다소 높인 것으로 나타났고, 그 결과 결정립계의 밀도를 감소시켜 확산방지막으로서의 특성을 향상시킨 것으로 사료된다. 또한 질소첨가는 실리사이드내의 금속과 실리콘과의 비를 변화시켜 확산방지막의 특성에 영향을 미친 것으로 보인다. 본 실험에서 조사된 확산방지막 중에서는 Mo-Si-N박막이 Cu와 Si간의 확산을 가장 효과적으로 저지시킨 것으로 나타났으며 650℃-30분까지 안정한 특성을 보였다. In this study, diffusion barrier properties of 1000 Å thick molybdenum compound(Mo, Mo-N, MoSi₂, Mo-Si-N) films were investigated using sheet resistance measurement, X-ray diffraction(XRD), X-ray photoelectron spectroscopy(XPS), Scanning electron microscopy(SEM), and Rutherford back-scattering spectrometry(RBS). Each barrier material was deposited by the dc magnetron sputtering and annealed at 300-800℃ for 30 min in vacuum. Mo and MoSi₂ barrier were failed at low temperatures due to Cu diffusion through grain boundaries and defects in Mo thin film and the reaction of Cu with Si within MoSi₂, respectively. A failure temperature could be raised to 650℃-30 min in the Mo barrier system and to 700℃-30 min in the Mo-silicide system by replacing Mo and MoSi₂ with Mo-N and Mo-Si-N, respectively. The crystallization temperature in the Mo-silicide film was raised by the addition of N₂. It is considered that not only the N₂ stuffing effect but also the variation of crystallization temperature affects the reaction of Cu with Si within Mo-silicide. It is found that Mo-Si-N is the more effective barrier than Mo, MoSi₂, or Mo-N to copper penetration preventing Cu reaction with the substrate for 30℃min at a temperature higher than 650℃.
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갓 (Brassica juncea) 품종구분을 위한 ITS 영역 및 MITE Family 정보를 이용한 분자표지 개발
양기웅(Kiwoung Yang),이고은(Go-eun Yi),아리프 하산 칸 로빈(Arif Hasan Khan Robin),정남희(Namhee Jeong),이용혁(Yong-Hyuk Lee),박종인(Jongin Park),김회택(Hoyteak Kim),정미영(Mi-Young Chung),노일섭(Ill-Sup Nou) 한국원예학회 2016 원예과학기술지 Vol.34 No.2
Brassica juncea (2n = 4x = 36, AABB genome, 1,068 Mb) is a U’s triangle species and an amphidiploid derivative of B. rapa and B. nigra. Fifteen varieties were used to study the ITS (internal transcribed spacer) regions of ribosomal DNA and MITEs (miniature inverted-repeat transposable elements) with a view of developing specific molecular markers. ITSs and MITEs are an excellent resource for developing DNA markers for genomics and evolutionary studies because most of them are stably inherited and present in high copy numbers. The ITS (ITS1 and ITS2) sequence was compared with the consensus sequence of B. rapa and B. nigra. Variation in ITS1 created two separate groups among 15 varieties, with 10 varieties in one group and 5 in the other. Phylogenetic analysis revealed two major clusters for those 10 and 5 varieties. Among the 160 different MITE primers used to evaluate the selected 15 varieties of B. juncea, 70 were related to the Stowaway, 79 to the Tourist, 6 to the hAT, and 5 to the Mutator super-families of MITEs. Of 160 markers examined, 32 were found to be polymorphic when fifteen different varieties of B. juncea were evaluated. The variety ‘Blackgat’ was different from the other mustard varieties with respect to both phenotype and genotype. The diversity of 47 additional accessions could be verified using eight selected molecular markers derived from MITE family sequences. The polymorphic markers identified in this study can be used for varietal classification, variety protection, and other breeding purposes.
ScienceON
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