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한의학 칠정(七情)에 기반을 둔 핵심감정평가척도 개발을 위한 예비 연구
이고은 ( Go Eun Lee ),박보영 ( Bo Young Park ),김향이 ( Hang Yi Kim ),유영수 ( Yeoung Su Lyu ),강형원 ( Hyung Won Kang ) 대한한방신경정신과학회 2014 동의신경정신과학회지 Vol.25 No.1
Objectives: The aim of this study is to investigate the core emotion assessment instrument based on the Chiljeong. Methods: We searched for a literature review of oriental medicine and established the operational definition. Based on the existing psychological scale measurement, we selected the items and analyzed the reliability through a two-step preliminary study. Results: 1) Through the analysis of reliability after the two-step preliminary study (first: 170 items, n=63, second: 152 items, n=42), we had excellent internal consistency (Cronbach``s alpha > 0.70) as well as good test-retest reliability (>0.70). Thus, we demonstrated that this scale had reliability. 2) Analyzing the correlation of each emotion, we had a comparatively strong positive-correlation (>0.6), except for Hui, and found a particularly high correlation of Sa, U and Bi. Conclusions: We developed the final 141 items of the core emotion assessment instrument through the two-time preliminary study. In the future, the main study on the clinical and non clinical groups of various ages should be continued.
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화병환자의 불면증상에 대한 침치료 유효성 평가연구: 환자-평가자 눈가림, 무작위배정, 일반대조군 임상시험
이고은 ( Go Eun Lee ),김남권 ( Nam Kwon Kim ),김향이 ( Hang Yi Kim ),강형원 ( Hyung Won Kang ) 대한한방신경정신과학회 2012 동의신경정신과학회지 Vol.23 No.1
Objectives: The purpose of this research is to examine the effects of acupuncture treatment on Hwa-byung patient`s insomnia. Methods: The study was performed through a patient-assessor blind, randomized, placebo-controlled trial in which the volunteers, data collectors and analysts were unaware of individuals who were receiving the treatment. A total of thirty-seven volunteers were divided into 2 groups. 18 subjects were placed into a trial group and 19 subjects into a control group by a randomization table. The trial group was treated on bilateral Shigu, Ahnmyun, B62 (Shinmaek), and K6 (Chohae), while the control group was not given any other treatment. The ISI(Insomnia Severity Scale) was measured as the 1st evaluative instrument, and then a comparative analysis was conducted by comparing the results with those measured by a Likert scale for major symptoms of Hwa-byung, PSQI, Hwa-byung scale, BDI, STAI, and STAXI. Results: In the ISI, PSQI, and the Likert scale regarding major symptoms of Hwa-byung, Hwa-byung scale, BDI, and STAI, more significant decreases were found in those concerning the trial group compared to those of the control group from a statistical perspective. However, in the STAXI, regarding the trial group, there was no significant decrease compared to the control group. Conclusions: The results suggest that acupuncture treatment is effective in the treatment regarding Hwa-byung patients who suffer from insomnia.
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갓 (Brassica juncea) 품종구분을 위한 ITS 영역 및 MITE Family 정보를 이용한 분자표지 개발
양기웅(Kiwoung Yang),이고은(Go-eun Yi),아리프 하산 칸 로빈(Arif Hasan Khan Robin),정남희(Namhee Jeong),이용혁(Yong-Hyuk Lee),박종인(Jongin Park),김회택(Hoyteak Kim),정미영(Mi-Young Chung),노일섭(Ill-Sup Nou) 한국원예학회 2016 원예과학기술지 Vol.34 No.2
갓(Brassica juncea; 2n = 4x = 36, AABB genome, 1,068Mb)은 U’s triangle의 배추와 흑겨자 사이의 복이배체 작물로 구분한다. 본 연구는 갓 15 품종의 ribosomal DNA ITS 영역과 MITE를 이용하여 갓의 유연관계 및 품종구분 분자표지를 확인하였다. Ribosomal DNA ITS 영역은 종 및 품종의 유연관계를 알아보는 연구로 많이 사용되고 있어서, 이를 이용하여 갓 15 품종의 유연관계를 알아보았다. 또한, MITE는 매우 많은 copy 수를 가지고 있고, 유전적으로 안정적이기 때문에 유전체 및 진화연구에 매우 적합한 재료이다. MITE를 이용한 갓의 품종구분 분자표지를 확인하기 위해 MITE super-families 중 Stowaway(BraSto) 관련 70점, Tourist(BraTo) 관련 79점, hAT(BrahAT) 관련 6점, Mutator(BraMu) 관련 5점으로 품종구분 표지를 알아보았다. 총 160점의 분자표지 중 32점이 갓 15 품종에서 뚜렷한 다형성을 보였다. 특히, 흑갓은 표현형뿐만 아니라 유전자형도 매우 다르게 나타났다. 또한 8점의 MITE 분자표지를 활용하여 47점의 유전자원에서 다형성 및 품종구분 표지로의 활용 가능성을 확인하였다. 이러한 다형성 표지들은 갓의 품종구분 및 품종 보호에 매우 유용하게 사용할 수 있을 것이라 기대한다. Brassica juncea (2n = 4x = 36, AABB genome, 1,068 Mb) is a U’s triangle species and an amphidiploid derivative of B. rapa and B. nigra. Fifteen varieties were used to study the ITS (internal transcribed spacer) regions of ribosomal DNA and MITEs (miniature inverted-repeat transposable elements) with a view of developing specific molecular markers. ITSs and MITEs are an excellent resource for developing DNA markers for genomics and evolutionary studies because most of them are stably inherited and present in high copy numbers. The ITS (ITS1 and ITS2) sequence was compared with the consensus sequence of B. rapa and B. nigra. Variation in ITS1 created two separate groups among 15 varieties, with 10 varieties in one group and 5 in the other. Phylogenetic analysis revealed two major clusters for those 10 and 5 varieties. Among the 160 different MITE primers used to evaluate the selected 15 varieties of B. juncea, 70 were related to the Stowaway, 79 to the Tourist, 6 to the hAT, and 5 to the Mutator super-families of MITEs. Of 160 markers examined, 32 were found to be polymorphic when fifteen different varieties of B. juncea were evaluated. The variety ‘Blackgat’ was different from the other mustard varieties with respect to both phenotype and genotype. The diversity of 47 additional accessions could be verified using eight selected molecular markers derived from MITE family sequences. The polymorphic markers identified in this study can be used for varietal classification, variety protection, and other breeding purposes.
ScienceON
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