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RISS 인기검색어
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- 저자 : 윤성철(Sung-Chul Yun) (검색결과 22 건)
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윤성철 ( Sung Chul Yun ) 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3
Ozone effects were studied by plant growth chamber to evaluate the impact of ozone (O₃) on the physiology of two hot pepper, Capsicum annuum L., cultivars, `Dabotab` and `Buchon`. Forty-day old plants with 5~7 leaves were exposed to O₃ of <20 and 150 nL/L for 8 h/d for 3 days. Net photosynthesis and stomatal conductance were measured and foliar injury was described. Foliar damage due to the treated O₃ was different from the varieties.`Dabotab` was most sensitive to O₃ and `Buchon` was resistant. Symptom of ozone damage on the leaves was bifacial necrosis. Decreases of net photosynthesis by O₃ were 56% and 40% on `Dabotab` and `Buchon`, respectively. Decreases of stomatal conductance by O₃ were 66% and 63% on each variety. O₃ damage on net photosynthesis was started at the low levels of light on the two hot peppers. In addition, assimilation-internal CO₂ concentration curves were not different from the two varieties. In conclusion, O₃ closed the stomata and decrease net photosynthesis on hot peppers regardless of the ozone sensitivity on leaf injury, but the difference of ecophysiological responses between the two varieties was not found clearly.
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윤성철(Sung Chul Yun),조종태(Jong Tae Cho),이재균(Jae Gyun Lee),김용진(Yong Jin Kim) 대한내과학회 1998 대한내과학회지 Vol.55 No.3
Background: A few researches on apoptosis in proliferative glomerulonephritis including lupus nephritis, have been reported on the basis of animal experimental models and human renal biopsy tissues. Systemic lupus erythematosus has been reported as o disease with an apoptosis defect of lymphocyte, so that it leads to the decreased immunologic tolerance and activated autoimmunity. The lupus nephritis is frequently accompanied with a interstitial fibrosis and tubuloepithelial change, as compared to other nephitis. In addition to that, the pathologic evolution from one class to other class is also feasible and these things make us to think that the lupus nephritis is tightly linked to the apoptosis. Method: We evaluated the relationship between the apoptosis and pathologic characteristics, which was focused on class IV, in 22 patients with clinically and biopsy proven lupus nephritis by comparing with control specimens. The study on the apoptosis was observed through in situ DNA nick end-labeling method and the review on the medical history. Results: 1) An apoptosis in glomerulus was observed in only five patients (zero in Class II, 50% in Class IV) of proliferative glomerulonephritis. But 14 patients (50% in Class II, 75% in Class IV) were detected on the site of tubulo-interstitium. 2) The apoptosis detected in tubulo-interstitial area was significantly correlated with nephrotie syndrome (P<0.025), but correlations with the complement serum level, renal function and the used amount of steroid was not determined. Conclusion: The apoptosis of glomerulus in the lupus nephritis was observed in 50% of proliferative cases (especially, in class IV), but in the area of tubulointerstitium, the higher rate (more than 50%) of apoptotic expressions were observed in class II and class IV. The observation on apaptosis in renal tissue could be another prognostic factor.
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윤성철(Sung Chul Yun),윤윤수(Yun Soo Yun),빈기태(Khi Tai Bhin),신영태(Young Tai Shin) 대한내과학회 1996 대한내과학회지 Vol.51 No.5
Objectives: Interleukin-6 (IL-6) is a pleiotrophic cytokines which is related to immune system activation, acute phase reactants, hematopoietic regulatory function, and cell growth as well as the first cytokine that may be directly related to pathogenesis of autoimmune disease, as systemic lupus erythematosus. The production site of IL-6 has been confined to T cell, B cell, macrophage, fibroblast, myeloma cell, myxoma cell, endothelial cell, especially mesangial cell and other glomerular cell in the kidney until now. However, recently uroepithelial cell stimulated by the bacteria and renal tublar cell damaged by immunologic injury were reported to be a another possible production site of IL-6. Therefore, Authors chose cases of lupus nephritis that was confirmed by renal biopsy. Methods: We observed the expression of IL-6 by using the immunohistochemical staining and tried to estimate the role of IL-6. The patients classified as class 4, 5 that had a strong proliferative change histologically were mainly selected as specimens for a research. The specimens were studied immunohistologically and its clinical data were compared in various ways. Results: 1) Positive IL-6 expression was observed in 16 cases (80%) of total 20 cases. IL-6 positive cases of glomerulus were accompanied with the strong IL-6 expression of renal tubules all the time. 2) The stronger IL-6 expression more than (+ +) was confirmed in 7 cases of 12 class 4 cases (87.5%). 3) The incidence of nephrotic syndrome and the case of decreased complement (C3, C4) were more prevalent in the group of positive IL-6 when compared to the group of negative IL-6. Conclusions: IL-6 has a strong association with renal tubular cells and play an important role in cellular proliferation of glomerulus as well as in disease activity of lupus nephritis.
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원발성 간암환자에서 관찰된 99mTc - DISIDA의 비장 섭취
윤성철(Sung Chul Yun),이예봉(Ye Bong Lee),김성진(Sung Jin Kim),박언휘(Eun Hwi Park),방인숙(In Sook Bang),김홍주(Heung Joo Kim),이원재(Won Jae Lee) 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.1
We report a case of hepatorna which had a suggested splenic metastasis in a 64 year-old male patient. Hepatoma proved by abdominal CT (Cornputed Tomography) and considerable accumulation of 99mTc-DISIDA was noted in a enlarged sp]een. We suggests the splenic uptake of the radionuclide corresponded to the diffuse metasfasis of hepatorna to spleen.
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비후된 심근에서의 유세포 분석을 통한 Nuclear DNA 의 관찰
김성진(Sung Jin Kim),방인숙(In Sook Bang),박언휘(Eun Hwi Park),김홍주(Heung Joo Kim),김도헌(De Heon Kim),이예봉(Ye Bong Lee),윤성철(Sung Chul Yun),한동선(Dong Sun Han),김성숙(Sung Sook Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.2
Background: During the development of the cardiac hypertrophy, cytoplasmic contents of cardiac myocyte usually increase, but the changes in the nucleus of myocytes are not well understood. Therefore, we used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy from human autopsy heart tissue. Besides, we also tried to see the DNA ploidy of developing heart of fetus. Method: Nine hypertrophied human hearts ranging from 350-620 g (Group II: 350-500 g, Group III: > 500 g) and 9 control hearts (Group I: 250-350 g) which we could obtain after the legal autopsy were studied with flow cytometric analysis. And 3 fetal hearts of 20-24 gestational weeks (Group IV) were also studied. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparation using Modified Hedley and Vindelov Method and staining with Krishan staining buffer. Results: In control hearts (Group I), Flow cytometric analysis showed normal Diploidy pattern in all tissue. However, in cardiac hypertrophy, Non-Diploidy pattern was predominant (3 out of 5 specimen in Group II, and all 4 specimen in Group III) and Tetraploidy was usually observed in this abnormal ploidy pattern, but Aneuploidy was also seen in 2 cases of severe hypertrophy of Group III. Proliferative indexes increased progressively in Group I, II, and III (17.8±5.55, 24.8±7.4%, and 36.4±5.2%, respectively) (p<0.05 between Group I and II, and p<0.01 between Group I k III). Normal growing heart tissue of fetus showed normal Diploidy pattern. Conclusions: In cardiac hypertrophy, abnormal nuclear DNA ploidy pattern can be obseved. This finding might say that, during the genesis of cardiac hyper- trophy, not only cytoplasmic change but also DNA synthesis in the nucleus occurs, but G2 phase arrest happens without further mitosis.
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자연발증 고혈압쥐의 신장과 심장에서의 아포프토시스에 대한 비교 연구
김용진(Yong Jin Kim),윤성철(Sung Chul Yun),김영권(Young Kwon Kim) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3
N/A Backgronnd: The terminal features of hypertensive target organ damage include decrease in the kidney size and increase in the heart size and wall thickness. Increased apoptosis has been known in the hypertensive nephrosclerosis and in the hypertensive heart failure. We hypothesized that apoptosis may progress by different degrees in the kidneys and heart with hypertension being sustained. To test this hypothesis we examined apoptosis in the kidneys and hearts of spontaneously hypertensive rats(SHR) of various ages. In addition, we examined histopathology of the kidneys. Methods: The kidneys and hearts of 19 SHR were excised at the age of 16 weeks(n=4), 20 weeks (n=6), and 32 weeks(n=9). Sprague-Dawley rats(SDR, n=6) were also sacrificed at the age of 16-24 weeks. Degree of apoptosis was evaluated semi-quantitati-vely by counting the number of apoptotic nuclei, stained by TUNEL method, per high power field(x400). Light microscopic and electron microscopic examination of the kidneys were performed. Results: 1) In SHR kidneys, the number of apoptotic nuclei at the age of 16 weeks was similar to that in SDR kidneys(9.3±0.5 vs. 10.2±2.2, p=NS). However, the number was significantly, p<0.05, increased at the ages of 20 weeks and 32 weeks(31.5±4.4 and 34.1±4.0, respectively) as compared with that in SHR kidneys at the age of 16 weeks and that in SDH kidneys. 2) In SHR hearts, the number of apoptotic nuclei at the ages of 16, 20, and 32 weeks(4.0±1,2, 2.0±0.7, 1.9±0.4, respectively) was neither changed nor different significantly from that in SDR hearts(0.70.5, p=N5), although the heart of SHR was hypertrophied at the age of 32 weeks. 3) Apoptosis was detected most frequently in the outer medulla of the kidneys in SHB. Histopathologic findings were the segmented sclerosis of glomeruli (1/25-50 glomeruli), edema, vacuolization and decreased villi of tubular epithelial cells. The older the age of SHR was, the more severe histopathologic changes were found. Conclusion : The sustained hypertansion caused increased apoptosis in the kidneys but no increased apoptosis in the heart of SHR during the specified ages of our study. The longer the duration of hyper- tension was, the more apoptotic cells and the more severe histopathologic changes, mainly in the tubulo-interstitial area, were found in the kidneys. The most frequent site of apoptosis was the outer medulla. It is suggested that apoptosis in the kidneys begin earlier than that in the heart in hypertensive target organ damage.
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한국의 2011-2100년 기후변화가 고추 탄저병 살균제 살포에 미치는 영향
신정욱 ( Jeong Wook Shin ),윤성철 ( Sung Chul Yun ) 한국농림기상학회 2011 한국농림기상학회지 Vol.13 No.1
In order to predict the risk of anthracnose on hot pepper in the future, the projected climate data from SRES A1B scenario in South Korea were used with the modified anthracnose model to calculate Infection Risk (IR), which was to estimate the number of fungicide sprays. Based on daily temperature and precipitation, the anthracnose model resulted in an empirical relationship that IR = (Daily temperature - 16℃) × 0.07 + (Daily precipitation × 0.11). For 135 locations in South Korea, the total number of fungicide sprays needed from 2011 to 2100 was 12,150, indicating a complicated change with an overall increase in anthracnose development in all locations until 2100. In particular, radical changes in anthracnose development were predicted at Yeongdeok, Yeongyang, and Uiseong, whereas gradual changes were predicted at Heongsung, Hamyang and Taean. The eastern counties of Gyeongbuk Province, which ar the major plantation area in these days, would be the place with the highest disease pressure in the future. In addition, the years of 2058, 61, 78 and 2096 will be most severe, requiring 8-11 times of fungicide spraying. The GIS maps show that the mountain areas of Jeonbuk and Chungbuk Province would have the least disease pressure of anthracnose in the future.
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