마우스 비장세포에서 Ginsenoside Rp1의 세포자멸사 유도

오영균,주홍구,Oh, Young-Kyun,Joo, Hong-Gu 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.3

Ginsenoside Rp1 is one of ginseng saponins with chemotherapeutic activity. In this study, we investigated the effects of Rp1 on spleen cells. Spleen is a major immune organ consisted of crucial immune cells, such as T lymphocytes, B lymphocytes, natural killer cells, and some antigen-presenting cells. Although the anti-tumor potential of Rp1 was studied, the effects of Rp1 on immune cells have not investigated yet. A viability assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), flow cytometric analysis, Western blot analysis were used to detect cellular changes on Rp1-treated spleen cells. MTT assay showed that Rp1 decreased the viability of spleen cells. To further investigate the effects of Rp1 on activated spleen cells, we treated lipopolysaccharide (LPS) as a representative inflammatory agent and Rp1 on spleen cells in a combination. The surface expression levels of activation markers for lymphocytes, CD25 and CD69 were measured. Apoptotic analysis revealed the cytotoxic effects of Rp1 on both na$\ddot{i}$ve and activated cells, and the expression pattern of some apoptosis-related proteins was correlated to apoptotic events of cells. Taken together, ginsenoside Rp1 increases the cellular death of spleen cells and also inhibits the LPS-induced activation of spleen cells.

교화서의 그림과 행실도의 의미

오영균(Oh, Young-Kyun) 부산대학교 인문학연구소 2017 코기토 Vol.- No.82

시각적 이미지를 매개로 하는 그림은 동아시아의 전통에서 오랫동안 책의 일부로 기능했을 뿐만 아니라, 글로 된 텍스트가 성립됐을 때부터 글과 함께 공존해왔다. 따라서 그림과 글 사이의 관계는 무엇이며 어떤 방식로 조응하여 “읽기”를 완성하는가 하는 문제는 책과 독서의 문화를 연구하는 이들에게 중요한 과제가 아닐 수 없다. 이 글은 이런 맥락에서 『삼강행실도』라는 교화서에 들어간 그림의 의미를 살핀다. 『삼강행실도』는 충신, 효자, 열녀의 귀감이 되는 얘기를 골라 글로 기록하고 그것을 그림으로 그 장면들을 형상화해 책으로 낸 것이다. 그렇다면 『삼강행실도』의 도화(圖畫)는 어떻게 그 교화의 대상으로 상정된 우부우부(愚夫愚婦)의 문맹독서(文盲讀書)에 기여했을 것인가? 이 문제를 다루기 위해 여기에서는 도(圖)와 화(畫) 간의 발생적 구분을 상기한다. 전통적으로 도(圖)는 구조적 골조를 시각화하여 독자를 가르치는 다분히 추상적인 그림으로, 화(畫)는 장면 장면의 이미지를 가지고, 보는 이의 감흥과 긴장을 불러일으키는 현물적 그림으로 구분되어 발전해왔다. 이런 맥락에서 이 글은 『삼강행실도』라는 교화서에 들어간 그림은 글을 모르는 대중이 도덕적 행위를 행하는 구체적 방법을 도식화한 예도(禮圖)로 변모한다는 가설을 제시한다. Illustrations, or the medium of visual images, have long been a part of the book in East Asia, as well as coexisted with written scripts ever since the beginning of literary texts. The nature of the relation between written texts and illustrations, and how they interact to complete “reading” is thus a one of the critical questions to the students of the culture of books and reading. In this context, this article explores the significance of illustrations included in the Samgang haengsil-to [Illustrated Guide to the Three Relations], a Chosŏn-Korean moral primer. The Samgang haengsil-to is a book recorded stories of exemplary loyal subjects, filial sons, and devoted wives with added illustrations that represented the scenes of their moral actions. How did the illustration of the Samgang haengsil-to, which was in fact intended to morally transform the general public often designated as “ignorant men and women” in the Confucian literature, contribute to the reading of the illiterate? Recalling the conceptual and generic difference between to (diagram) and hwa (picture) that persisted traditionally, I propose that the illustrations of the Samgang haengsil-to shifted from hwa, realistic pictures of concrete scenes in life with which to evoke the viewer’s tension and affects, to to, principally abstract delineation of structural construction with which to instruct readers. In this sense, the illustrations of the Samgang haengsil-to became yedo (ritualistic manual) for commoners, formularizing concrete methods for the illiterate to perform moral actions.

마우스 비장세포에서 Ginsenoside Rp1의 세포자멸사 유도

오영균(Young Kyun Oh),주홍구(Hong Gu Joo) 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.3

Ginsenoside Rp1 is one of ginseng saponins with chemotherapeutic activity. In this study, we investigated the effects of Rp1 on spleen cells. Spleen is a major immune organ consisted of crucial immune cells, such as T lymphocytes,B lymphocytes, natural killer cells, and some antigen-presenting cells. Although the anti-tumor potential of Rp1 was studied,the effects of Rp1 on immune cells have not investigated yet. A viability assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), flow cytometric analysis, Western blot analysis were used to detect cellular changes on Rp1-treated spleen cells. MTT assay showed that Rp1 decreased the viability of spleen cells. To further investigate the effects of Rp1 on activated spleen cells, we treated lipopolysaccharide (LPS) as a representative inflammatory agent and Rp1 on spleen cells in a combination. The surface expression levels of activation markers for lymphocytes, CD25and CD69 were measured. Apoptotic analysis revealed the cytotoxic effects of Rp1 on both naive and activated cells,and the expression pattern of some apoptosis-related proteins was correlated to apoptotic events of cells. Taken together,ginsenoside Rp1 increases the cellular death of spleen cells and also inhibits the LPS-induced activation of spleen cells.

지역 환경청 인력규모 추계에 관한 연구

오영균(Oh young kyun) 한국지방정부학회 2015 지방정부연구 Vol.18 No.4

공공부문에 지속적인 논란이 되고 있는 분야중 하나가 적절한 인력이 투입되고 있는가이다. 비판적인 입장에서는 보다 적은 인력으로 충분히 일을 잘 할 수 있는데 계속 추가적인 인력소요를 제기한다는 것이며, 다른 입장에서는 필요인력이 부족하여 수행하여야 할 공공서비스를 제대로 공급할 수 없다는 것이다. 사례에 따라 맞고 틀림이 달라질 것이기에 일반적으로 어느 견해가 타당하다고 말할 수는 없을 것이다. 하지만 이러한 논쟁이 지속되는 가운데 적정인력은 어떠한 기준에서 어떻게 판단할 수 있는지에 대한 실증연구의 축적 없이 공공부문의 효율성 향상을 추진하는 것은 바람직하지 않다. 왜냐하면 인력은 예산과 더불어 핵심투입요소이기 때문에 산출이 일정할 경우 투입의 적절성이 효율성을 좌우할 수 있기 때문이다. 본 연구는 선행연구에서 현재 매우 중요한 업무임에도 불구하고 인력이 부족하여 필요한 서비스가 제공되고 있지 못하다고 지적하고 있는 환경영향평가 사후관리 투입 인력규모의 적정에 관한 분석연구이다. 특별히 행정학에서 오랫동안 다루어왔던 이론적 내용을 실제 분석에 적용하여 업무량과 필요시간을 분석하고 필요인력을 추계함으로써 직무분석의 연구결과를 축적하고 정책적으로 필요인력규모에 대한 적정성 판단에 기여하고자 하였다. 물론 본 연구는 특정분야에 대한 연구로서 일반화의 한계를 지니고 있다. 하지만, 향후 관련 후속 연구들이 축적되면 공공부문의 효율성 향상노력에 크게 기여할 수 있을 것으로 기대한다. Proper distribution of manpower in public sector is the one of the consistent hot issues. There are two perspectives. One is negative stand on the reason of increasing demand in public sector exceeding real need. the other is positive stand on the ground of the sufficient manpower is necessary for proper public service. We can not make clear decision on this controversy, because the correct answer depends on the specific case itself. However, it is obvious that we need empirical research on the criteria and method to help correct judgement. Man power scale is critical factor to promote efficiency in all kind of organization. This article is to analyze necessary manpower scale in doing environment impact evaluation management service of local environment offices. Especially we aims to cumulate research outputs of task analysis and to draw useful policy implication. However, this research has a few limits to be generalized due to dealing with special case. Nevertheless, it is expected to give useful information and policy implication related afterward research to promote public efficiency.

지역인적자원개발정책의 평가를 위한 지표의 설계에 관한 연구

오영균 ( Young Kyun Oh ),고경훈 ( Kyeong Hun Go ) 한국지방행정연구원 2007 地方行政硏究 Vol.21 No.3

지방정부 3.0 이론과 과제

오영균 ( Young Kyun Oh ) 한국지방행정연구원 2013 地方行政硏究 Vol.27 No.2

Local Government 3.0 is an extention of Government 3.0. So we have to understand Gov.3.0 concept and contents before discussing Local Gov.3.0. Gov 3.0 is a new paradigm for creative economy and ordered public service by proactive information sharing and cooperative tasking among departments and other public actors. However, We must keep in mind there are many obstacles to overcome for information sharing, openness, participation and ordered service. Web2.0, Cloud computing and Big data analysis are not sufficient, if not reforming law and institution. above all, Upper level coordination tower can also be operative whenever it is needed. Specially, we really wish that we can overcome moral hazard, we should work out a design for external and internal incentive system. In my analysis, New PCRM can be a best alternative, because we can control demand and supply capacity to find rational equilibrium with it.

주민복리를 위한 적극행정의 정당성과 한계 : 사전컨설팅감사제도를 중심으로

오영균 ( Oh Young Kyun ) 한국지방행정연구원 2017 地方行政硏究 Vol.31 No.2

This paper aims at examining the pre consulting audit issues and justification for positive administration in local governments. Fundamently, this study adopts literatures to find the hudles of pre consulting audit against positive administration at local levels. This study intends to find out the theoretical ground for local government pre consulting audit to enhance the assertive public administration. The findings are as follows: First, principle-agent theory and system theory logic can be the Justification of pre consulting audit. Secondly, though there are some dissents, we can find the necessity and usefulness of pre consulting audit. Finally, if we enhance the usefulness of pre consulting audit, we should develop the capacity of audit department and its members.

지방의회 지원기구 직무역량 및 직무만족 제고를 위한 전략적 인적자원관리요인에 관한 연구: 경기도 의회사무처 사례를 중심으로

오영균 ( Young Kyun Oh ) 한국지방행정연구원 2015 地方行政硏究 Vol.29 No.3

The success or failure of local autonomy is dependent on the competence of each component members. Therefore, each organization needs outstanding specialists with professional knowledge and site-specific perception. Accordingly, the realizing the importance of human resources, put a lot of effort into developing personnel to strengthen competitiveness through improvement of productivity and differentiation. The aim of this study was to ascertain the interrelationships between human resource factors and Gyeonggido assembly officer``s performance competence to improve the performance achievement through the strategic human resource management. Data were collected from 159 employees from august 1st to 7th, 2015. Multiple regression analysis was employed to test hypotheses. the results showed that two independent variables of strategic human resource management has a positive influence to employees with job competence and job satisfaction.

지방재정특성이 커뮤니티웰빙에 미치는 영향에 관한 연구

오영균 ( Oh Young Kyun ) 한국지방행정연구원 2017 地方行政硏究 Vol.31 No.3

This paper basically aims at examining how do the local fiscal character affect community well-being level of local residents. Fundermantly, we adopts literatures study to find the determinants of well-being before designing the model. This study intends to find out the theoretical ground for local government fiscal character to enhance the satisfaction of residents in various fields such as environment, education etc. The findings are as follows: First, local government fiscal scale and public service can be essential factors for local well-being level. Secondly, though there can be some dissents, the self funding of local government itself is not the factor. it means local government may use diverse funds of central government for their residents` well-being.

지방정부 자체평가에서의 주민만족도분석에 관한 사례 연구

오영균 ( Young Kyun Oh ) 한국지방행정연구원 2006 地方行政硏究 Vol.20 No.3