전북지역에서 발생한 돼지콜레라의 역학적 특성

엄성심 ( Sung Shim Eum ),이정원 ( Jeoung Won Lee ),서이원 ( Won Seo Lee ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3

Classical swine fever(CSF) was confirmed in 19 herds in Jeunbuk provence(Iksan, Gimje, Wanju, Buan, and Jangsu) in Korea between March and May, 2003 and 10,263 pigs were slaughtered. Pigs contacted with CSF virus in primary outbreak farm show fever, reduced appetite, arched back and chill in company with sever respirative sign and then most infected farms also were observed to fever, reduced appetite, sudden death, and leukopenia(101 pigs). In order to detecting infectious pig with CSF virus, A total of 555 pigs were inspected in 65 herds and blood samples were collected and serological test(ELISA), antigen ELISA, and reverse transcription-polymerase chain reaction(RT-PCR) had been done. Positive rate were 74%(410 pigs) in antibody ELISA, 2%(11 pigs) in antigen ELISA and 33%(182 pigs) in RT-PCR, respectively. As shown that the RT-PCR was useful than the ELISA for determining CSF virus in blood, meat, and other organs.

IBDV (SH/92)의 인공감염에 의한 닭 면역장기의 병리조직학적 연구

엄성심 ( Sung Shim Eum ),김범석 ( Bum Seok Kim ),임채웅 ( Chae Woong Lim ),임병무 ( Byung Moo Rim ),이호일 ( Ho Il Lee ),정동석 ( Dong Suk Chung ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.3

Sequential morphologic changes in the lymphoid organs were examined after ocular and cloacal inoculation in 3weekold chicks with a highly virulent strain (SH/92) of infectious bursal disease virus (IBDV). The infected chickens were sacrificed at 6, 12, 24, 48, 72, and 96 hrs post inoculation (P1), and thymus, harderian gland, ceacal tonsil, and spleen were observed. Histologically, the significant lesions were characterized by lymphocyte depletion and the earliest detectable changes were evident at 12 hrs PI. In thymic cortex, lymphoid depletion with apoptosis and prominent "tingible body macrophages" were observed. As the infection advanced, the lesions showed more severe changes. Dying cells were characterized either by capping of nuclear chromatin (apoptosis) or by cytoplasmic swelling (necrosis). In situ staining for apoptosis, some lymphoid cells revealed typical positive reaction, even the stainability was variable depend on every lymphoid organs. These results suggest that IBDV (SH/92) cause severe damage both primary and secondary lymphoid organs, and both T and B lymphocytes. Also the lymphoid depletion of these organs is caused by necrosis and apoptosis induced by IBDV.

전라북도 동부지역 사슴에서 진드기매개성 병원체에 대한 감염 실태 조사

엄성심 ( Sung Shim Eum ),고원석 ( Won Seuk Koh ),허철호 ( Cheal Ho Hur ),배정준 ( Joung Jun Bae ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.2

Ticks cause economic losses to the deer industry by decreasing the growth and production of the farmed animals. The mediation of ticks affects humans and animals by causing contagious disease both directly and indirectly. Blood from farmed deer from the areas near Jangsu branch was collected for screening of infectious protozoa and rickettsial disease. Seventy deer blood samples were collected from 30 different deer farms located in Jinan, Jangsu and Muju. This blood samples were used for blood slide smear examination and hematological analysis. DNA from these samples was extracted and was used for PCR analysis for detection of gene fragments of Theileria spp, Babesia spp, Anaplasma spp and Ehrlichia spp. In the blood slide smear examination and PCR analysis all samples did not show presence of protozoal and rickettsial diseases. Eight blood samples showed anemia, 1 sample showed iron deficiency and 7 samples showed regenerative anemia. Results for PCR analysis showed 2 samples were positive for T orientalis. All DNA samples were negative for Babesia spp, Anaplasma spp, and Ehrlichia spp.

전주지역 애완견에서 심장사상충, 개선충 및 모낭충 감염실태 조사

이정원 ( Jeoung Won Lee ),엄성심 ( Sung Shim Eum ),박인규 ( In Gyu Park ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.1

A survey on the prevalence of heartworms, scabies and demodicosis of 1,011 pet dogs in 5 animal hospitals Jeonju area was performed from June to October 2004. The result obtained from this experiment were summarized as follows; 1. The 1,011 pet dogs coming to help the animal hospital more than disease of pleonephritis 62(6.1%), enteritis 75(7.4%), dermatitis 62(6.1%), scabies 55(5.4%), heartworms 43(4.3%), tympanitis 41(4.1%), demodicosis 22(2.2%), gastritis 21(2.2%), fracture 9(0.9%), respectively. 2. The 307 examined pet dogs were heartworms antigen positive to 43(14.0%), Microfilaria 31(10.1%) and the kinds of mixed breeds 34 (antigen and Microfilaria 12), Jindo 19(antigen 7, Microfilaria 6), respectively. 3. Heartworms antigen positive of examined 34 pet dogs mean ages 4.3 and general blood cell count mean of WBC 20.5×103/㎣, RBC 61.3×106/㎣, HGB 6.27g/㎗, HCT 20.9%, PLT 13.0×103/㎣, MCV 33.2㎛3, MCH 39.4pg, MCHC 14.8g/㎗, RDW 280. 1%, MPV 9.2㎛3, respectively. but showed a few high normal value WBC, MCV, MCH and a low MCHC. 4. This experiment was 1,011 pet dogs to dermatitis 139(13.7%) of scabies 55(39.6%), demodicosis 22(15.8%), respectively.

산란계 밀집지역의 산란저하성 질병에 관한 연구

이정원 ( Jeoung Won Lee ),엄성심 ( Sung Shim Eum ),박인규 ( In Gyu Park ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.2

Newcastle disease(ND), infectious bronchitis(IB), low pathogenic avian influenza(LPAI) and fowl typhoid(FT) have been known as egg drop laying diseases because of the serious layer damage from mass zone layer. In this study, such egg drop laying diseases were investigated. To access this study, we performed to evaluate antibody titers in serum and isolated bacteria and virus from organs and feces on May, July and September in 2003. The distribution of ND from January to May, IB and LPAI from October to February of the next year, and FT from March to September were inspected by the question survey in 21 farms. ND revealed to be positive rates of 490 to 474(96.7%) in May, 510 to 506(99.2%) in July and 510 to 510(100%) in September with hemagglutination inhibition(HI) test. The mean antibody titers were 10.2, 9.9 and 10.2, respectively. With regard to IB, 484 out of 490 samples(98.7%) in May, 508 of 510(99.6%) in July and 509 of 510(99.8%) in September showed positive results and the mean antibody titers were gradually increased with 8.2, 9.0 and 9.4, respectively. According to HI test of LPAI, the positive results were shown in 442 of 480(92.1%), 394 of 494(79.8%) and 402 of 483(83.2%) in May, July and September, respectively. The mean antibody titers were decreased with 4.6, 4.3 and 4.0. The distribution of LPAI also elicited the positive rates of 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September as determined by ELISA and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. Compared ELISA results with HI test of LPAI the positive results were 480 to 422(92.1%), 475(99.0%), 494 to 394 (79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, the positive rate determined by ELISA was higher than that of HI test with 6.9, 18.4 and 14.5%, respectively. When performed RT-PCR for ND using organ and feces samples, the pathotypes were detected 5(15.6%) in May, 2(5.3%) in July, 2(7.1%) in September but there is no samples showing positive band for LPAI. In attempt to isolate Salmonella gallinarum, bacteria were obtained from 4 cases(12.5%) in May, 9(23.6%) in July, 5(17.8%) in September. Thus the highest rate for isolation revealed to be shown in July. When evaluated the antimicrobial susceptibility to 18 isolated strains of S. gallinarum, bacteria were sensitive to trimethoprim/sulfamethox(61.1%), kanamycin(55.5%), ampicillin(55.5%) and amoxacillin/clavulanic acid(55.5%), cephalothin(50.0%), but resistant to penicillin(88.9%), streptomycin(88.9%), erythromycin(83.4%) and tetracycline(61.1%). According to HI test of ND and LPAI using captured 164 wild Korean tree sparrows(Passer nontanus), the positive rates were 47.6% and 57.3%, and the mean HI titers were 5.32 and 4.02, respectively. 71(43.2%) and 58(35.3%) in captured sparrows also showed more than 4 titers for HI test to ND and LPAI, respectively. However, the attempt for isolation of viruses failed in all samples.

전북 남원지역 육계에서 살모넬라증에 대한 역학적 조사

정영미 ( Young Mee Jung ),김기주 ( Ki Joo Kim ),엄성심 ( Sung Shim Eum ),이지영 ( Ji Young Lee ),노영선 ( Young Sun Rho ),서석열 ( Surk Yul Seo ),박정배 ( Jong Bae Park ),이희문 ( Hee Moon Lee ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1

This survey was performed to investigate avian salmonellosis from broiler farms in Namwon area for 4 years. The whole samples from farms were 541 and chicken samples were 294. Out of 294, 83 cases were identified with avian salmonellosis for 4 years by various serological and biochemical tests. There was no outbreak of pullorum disease, but fowl typhoid occurred in 36 farms. The outbreak of avian salmonellosis occurred continuously regardless of seasons throughout the year. Avian salmonellosis mostly happened within 10 days of age which were 52 cases(63%). The selection of susceptible antibiotics has changed from Quinoline group in 2000 to amoxacillin+clavulanic acid(AmC).

도축돈의 담즙, 뇨 및 장관에서 장내세균의 분리 및 항생물질 감수성

허부홍 ( Boo Hong Hur ),서석열 ( Surk Yul Seo ),엄성심 ( Sung Shim Eum ),김진환 ( Jin Hwan Kim ),윤창용 ( Chang Yong Yoon ),조정곤 ( Jeong Gon Cho ),송희종 ( Hee Jong Song ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.3

Seventy-nine strains of Enterobacteriaceae isolated from 117 slaughtered pigs (bile, urine, small intestine, cecum and rectum) in 1995 were examined for biotypes and susceptibility to 19 antibiotics with MicroScan WalkAway 40/96. The obtained results were as follows: 1. Among the twenty-two species isolated from the samples, Proteus mirabilis, E. coli and Enterobacter cloacae were commonly encountered. 2. The distribution frequency of isolates from cecum, small intestine, rectum, bile, and urine was 31(38.8%), 25(31.3%), 18(22.8%), 3(3.7%), and 2(2.5%), respectively. 3. A majority of isolates were sensitive to 16 antibiotics, singly or in combination. And these isolates were commonly susceptible to various antibiotics such as Cp, Ts, Azt, Caz, To, Gm, Cfz, Crm, Am and Cfx, in order. Whereas the Salmonella spp was susceptible to Cf, Ti and Pi, and Proteus mirabilis to Imp, Tim, Cft and Cz. Meanwhile, no effect was found to Cf, Ak and Cax. 4. Among the antibiotic resistant strains, a total of 17 reistant patterns was noted and of these Ak Tim 45(57.0%), Ak Am Cf Cfx Cfz Tim 8(10.1%) and Ak Ti Tim 6(7.6%) were frequently encountered.