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Seventy-nine strains of Enterobacteriaceae isolated from 117 slaughtered pigs (bile, urine, small intestine, cecum and rectum) in 1995 were examined for biotypes and susceptibility to 19 antibiotics with MicroScan WalkAway 40/96. The obtained results were as follows: 1. Among the twenty-two species isolated from the samples, Proteus mirabilis, E. coli and Enterobacter cloacae were commonly encountered. 2. The distribution frequency of isolates from cecum, small intestine, rectum, bile, and urine was 31(38.8%), 25(31.3%), 18(22.8%), 3(3.7%), and 2(2.5%), respectively. 3. A majority of isolates were sensitive to 16 antibiotics, singly or in combination. And these isolates were commonly susceptible to various antibiotics such as Cp, Ts, Azt, Caz, To, Gm, Cfz, Crm, Am and Cfx, in order. Whereas the Salmonella spp was susceptible to Cf, Ti and Pi, and Proteus mirabilis to Imp, Tim, Cft and Cz. Meanwhile, no effect was found to Cf, Ak and Cax. 4. Among the antibiotic resistant strains, a total of 17 reistant patterns was noted and of these Ak Tim 45(57.0%), Ak Am Cf Cfx Cfz Tim 8(10.1%) and Ak Ti Tim 6(7.6%) were frequently encountered.
Total viable cells and lactose non-fermenting cells were counted from animal feedstuffs (n=65). And isolation of Gram negative lactose nonfermenting enterobacteria and antibiotics susceptibility of isolates were performed. 1. The ranges of total viable cells / lactose non fermenting in animal feedstuffs from Korean cattle were counted as 9×10-1×10/1×102-6×10, milking cow as 1×104-2×108/2×102-8×10, pig as 1×104-1×106/2×102-6×104, and chicken as 7×l04-1×109/4×102-1×105 cfu/g, respectively. 2. Among the 214 isolates from feedstuffs, 87 from Chinan(n=23), 66 from Changsu (n=23) and 61 from Mooju(n=19) were isolated. Of these isolates, 60 from pigs (n=19), 51 from milking cows(n=15), 45 from chikens(n=11) and 58 from Korean cattle(20) were isolated. 3. Among the 6 genuses of Gram negative lactose nonfermenting enterobacili, Sal-monella sp, Y pseudotuberculosis, Ent agglomerans and Sal choleraesuis were frequently encountered. 4. A majority of isolates were sensitive to 19 antibiotics, singly or in combination. These isoates were completely susceptible to Cp, Gm, Imp and Pi, 93% to Ak and To, 73% to Cax and Ts, 66% to Cit and Tim, 46-53% to Caz, Cf and Cz, 33-40% to Am, Azt, Cfz and Ti, and 6% to Cfx, in order, but not susceptible to Crm. 5. Among the antibiotic resistant strains, a total of 23 resistant patterns was noted, and of these Crm 40(18.7%), Am Cf Cfx Cfz Crm Ti 27(12.6%), each of Azt Ctx Crm and Azt Cax Caz Cft Cfx Crm 22(10.3%) were frequently encountered.
Prevalence of major legal communicable diseases in bovine and swine had been monitored in Jeonbuk province from year 2004 to 2008. At least 1 communicable disease had been reported in 687 heads from 68 bovine farms and 17 farms (25.0%) of the 68 positive farms had 1∼2 additional outbreaks during the surveillance. By disease, enzootic bovine leukosis, Johne`s disease and Akabane disease were occurred in 53 farms (582 heads), 14 farms (100 heads) and 1 farm (5 heads), respectively. Swine communicable diseases were occurred in 4,466 heads from 63 swine farms and 18 farms (28.6%) of the 63 positive farms had 1∼2 additional outbreaks during the surveillance. By disease, Aujeszky`s disease (AD), porcine epidemic diarrhea (PED), classical swine fever (CSF), porcine reproductive and respiratory syndrome (PRRS), porcine transmissible gastroenteritis (TGE), atrophic rhinitis (AR) and Japanese encephalitis in swine (JE) were occurred in 20 farms (70 heads), 20 farms (2,817 heads), 12 farms (258 heads), 6 farms (1,257 heads), 1 farm (50 heads), 1 farm (2 heads) and 1 farm (10 heads), respectively. In total, 10 communicable diseases (1 species of first-class, 3 species of second-class, and 6 species of third-class) were reported. The first-class diseases were CSF. Johne`s disease, and Aujeszky`s disease. JE was the second-class and Akabane disease, enzootic bovine leukosis (EBL), PED, PRRS, TGE and AR were third-class diseases.
Prevalence of major legal communicable diseases in chickens and ducks, which had occurred in Jeonbuk province from year 2004 to 2008. Total 283 farms 1,419,244 chickens and ducks have been affected by avian diseases. Specifically, fowl typhoid (FT) occurred in 92 farms 416,600 chickens, Marek`s disease (MD) in 45 farms 145,563, duck virus hepatitis (DVH) in 31 farms 199,200, infectious bursal disease (IBD) in 27 farms 113,220, infectious bronchitis (IB) in 27 farms 280,300, low pathogenic avian influenza (LPAI) in 26 farms 78,495, avian mycoplasmosis in 16 farms 103,774, Newcastle disease (ND) occurred in 11 farms 61,052, avian encephalomyelitis (AE) in 7 farms 21,000, Pullorum disease (PD) occurred in 1 farm 40. According to total analysis about major legal communicable diseases, 1 species of first-class legal communicable diseases have occurred, 3 species of second- class and 6 species of third-class all adding up to 10 species. In the first-class diseases, Newcastle disease have occurred. Pullorum and fowl typhoid, duck virus hepatitis in the second-class have occurred and as third-class diseases, Marek`s disease, Infectious bursal disease, Infectious bronchitis, avian mycoplasmosis, avian encephalomyelitis, low pathogenic avian influenza have occurred.
The prevalence of major zoonotic diseases such as tuberculosis, brucellosis and the highly pathogenic avian influenza (HPAI) in Jeonbuk province was monitored from 2004 to 2008. For tuberculosis, a total of 306 heads from 92 farms were positive during the surveillance period, which 228 heads were from 78 dairy farms and 78 heads from 14 Hanwoo farms. Based on the number of recurrent tuberculosis in 92 positive farms, 28 farms or 43.8% of the positive farms had 1-4 additional outbreaks during the surveillance. Based on brucellosis surveillance of 5,252 dairy cattle and 2,600,829 Hanwoo conducted during the same time period, 4,818 heads from 1,203 farms were positive for brucellosis, which 445 heads were from 111 dairy farms and 4,373 heads from 1,092 Hanwoo farms. Among the 1,203 positive farms, 473 farms or 39.3% of the positive farms had experienced 1-4 recurrent brucellosis during the surveillance. According to nationwide surveillance of HPAI, a total of 45 cases had been reported between 2004 and 2008. Among those outbreaks, 20 cases were reported in Jeonbuk province and 3 cases in 2006 and 17 case in 2008. For the regional distribution of 20 cases in Jeonbuk, 4 cases (48,490 chickens), 4 cases (23,066 chickens 66(1) and ducks 23,000(3)), 11 cases (183,077 chickens 63,077 (10) and quails 120,000 (1)), and 1 case (9,000 ducks) were reported in Iksan, Jeongeup, Gimje and Sunchang, respectively.
The purpose of the present survey was to evaluate the antibiotic residues in meats and internal organs such as muscle, liver, heart, kidney and spleen of cattle(n=59) and pigs (n=115). The EEC-4-plate-method were employed. The results were obtained as follows: 1. In BS 6.0, BS 7.2 and BS 8.0 used as media to detect antibiotic residues, the zone(M±SD, cm) of bacterial growth inhibition was narrow(1.40±0) in meats, whereas the zone was wide(1.69±0.25-1.88±0.23 and 1.58±0.18-1.86±0.15 in cattle and pigs, respectively) in internal organs. But in SL 8.0, it was difficult to detect the zones(0-1.40±0) of both meats and internal organs. 2. Residues of antibiotic in beef and pork were rarely detected in BS 6.0, BS 7.2 and BS 8.0(range 1.7-11.9% and 2.6-4.3%, respectively), whereas residual percentages of internal organs were relatively higher(range 69.5-96.6% and 43.5-84.3%, respectively). But in SL 8.0, it was not detected in both beef or pork, whereas they were 0-13.6% and 0-4.3% in interanal organs.
허부홍 ( Boo Hong Hur ),전창권 ( Chang Kwun Jun ),이희문 ( Hee Moon Lee ),김용수 ( Yong Soo Kim ),김윤태 ( Yoon Tae Kim ),이정원 ( Jeung Won Lee ),최승옥 ( Seung Ok Choi ),안병목 ( Byeung Mok An ),송희종 ( Hee Jong Song ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.3
Anaplasmosis is most important tick-borne rickettsial diseases of domestic ruminants, with Anaplasma spp. as their respective causal agents. In order to the survey prevalence of anaplasmosis in Korean native goats, we examined the Giemsa`s blood filrms from 552 grazing and 188 nongrazing-goats in Chonbuk area Namwon, Imsil and Sunchang. The conclusive diagnosis was made by observing the characteristic marginal or central bodies in the red blood cells. The infection rate of grazine and nongrazing-goats with Anaplasma spp. was 71.7% and 8.5%, respectively. In order to study about the therapeutic effects of drug for 40 grazing-oats infected with Anaplasma spp. in mountain-area, Berenil(R)(diminazene acetate) was intramuscularly injected (0.5mℓ/10kg B.W.). After 1 month, we don`t detected the parasite in the blood films from 33 goats(82.5%), but no change of significant hematological values(PCV, ESR, WBC, RBC, MCH, MCHC, PLT, MPV, PDW and HB) was observed. These results indicated that the infection rate of Anaplasma spp. was higher on grazing than nongrazing-goats and Berenil(R) as an antianaplasmal drug is recommended.
In order to develop a good separation and simultaneous analysis of different sugar in an artificial mixed sugar solution, we analyzed 10 sugar components in an artificial mixed sugar solution composed of fructose, glucose, mannitol, sucrose, maltose, lactose, xylose, xylitol, erythritol, and trehalose with using HPLC-ELSD or HPLCRI. Separation and Quantification by I-IPLC-ELSL) was superior to those by 1-IPLC-R1 and detection sensitivity by HPLC-ELSD was higher then that by HPLC-RI as microgram(Ltg) level. 1. The units of minimal detectable limits were showed pg/me and ng/m by the HPLC-RI and I-IFLC-ELSD, respectively. 2. The condition of ELSD was drift tube temperature 82 C, N2 gas flow rate 2.10 SLPM, and column oven temperature 30 `C, respectively. Isolation and recovery rates of single sugar from the multiple sugar solution was higher at the condition (time: flow rate: D.W.: ACN MeOR, mm: ni/min: v: v: v) of linear gradient elution of mobile phase as 0: 1.00:15: 85: 0,1:1.00:6:90:4, 17:1.00:10:70:20, 23:1.00:15:85:0 and 35: 1.00:15:85:0, in order.
Fugi are eukaryotic, nonphotosynthetic, filamentous or unicellular organisms, most of which grow on nonliving materials as saphrophytes. The majority are therefore opportunistic pathogens and predisposing factors often contribute to the establishment of fungal infection. These include an alteration in the normal flora of the host by prolonged administration of antibiotics, immunosup-pression, concurrent infections, damage to the skin or mucous membranes, constantly moist areas of skin or the exposure to a large infective dose, and as with fungal spores. Fungi may cause a variety of diseases which may be due directly to fungal invasion of tissue or more often to the ingestion of toxins produces by fungi in growing, standing or stored grains and other animals feeds. In this experiment, contaminated fugi were isolated and identified from animal feedstuffs such as Korean cattle, milking cows, pigs and chickens. Twelve genues were isolated from animal feeds, they are 9 from Korean cattle and milking cows feeds, 6 from pigs feeds, and 10 from chickens feeds. Among them, most frequently encountered species was Yeast(56 strains), followed by Fusarium sp(41 strains), Aspergillus sp(20 strains), each of Microsporum sp and Trichophyton sp(17 strains), Penicillium sp(12 strains), in order. And also minority was isolated as Candida sp(4 strains), Trichoderma sp(3 strains), each of Epidermophytom sp and Absida sp(2 strains), and each of Sporothrix sp and Maduromyces sp(1 strain). Among the Aspergillus sp 20 isolates, A flavus(5 strains), A nidulans(4 strains), A fumigatus(3 strains), A glucans(3 strains), A niger(3 strains) and A terreus(2 strains) were identified.
This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.