간흡충 감염 흰쥐에 대한 알벤다졸의 치료 효과

손운목,장태식 인제대학교 1991 仁濟醫學 Vol.12 No.1

간흡충을 횐쥐에 실험 감염시킨 후 2일, 2주 및 5주에 여러가지 용량의 알벤다졸을 투여하여 충체의 각 발육단계 및 약제용량에 따른 살충 효과를 검토하고자 하였다. 감염 후 2일, 2주, 5주의 발육 단계별 살충율은 각각 26.2∼47.6(평균 39.3) %, 21.4∼54.8(평균 32.2) %,84.3-100(평균 91)%로, 유약충에 비해 성충에 대한 살충율이 높았다. 동일 치료군 내에서는 약제 투여량 및 방법에 따라 살충율의 차이가 뚜렷하지 않았고, 각 치료군 공통으로 2×200mg 용량에서 가장 높은 살충율을 나타내었다. 완전한 살충은 감염 5주 후 치료군의 2×200mg 투약군에서만 이루어졌다. This study was performed to evaluate the chemotherapeutic effects of albendazole against the larvae, juveniles and adults of Clonorchis sinensis. The metacercariae were obtained from Pseudorasbora parva by the pepsin digestion method, and 70 rats(Sprague-Dawley strain) were fed each with 50 metacercariae. On 60 rats various dosages of albendazole(2×2mg, 2×50mg, 200mg, 2×100mg, 2×200mg) were given at 2 days, 2 weeks and 5 weeks after infection while remaining 10 rats were kept untreated for use as control. The drug-treated rats and untreated control were sacrificed at 6 weeks after infection for the recovery of worms. The cure rates(lethal rates) obtained from worm recovery rates were used as the decision base of drug effects. Followings are summarized results. 1.The recovery rate of C. sinensis in control group was in the range from 60% to 100%, with overall average value of 84%. 2.The mean recovery rates of worms in treated groups are presented in the following Table. ◁표 삽입▷ (원문을 참조하세요) 3.The cure rates(lethal rates) of the groups treated at 2 days, 2 weeks and 5 weeks after infection were 26.2-47.6%(39.3% in average), 21.4-54.8%(32.3% in average) and 84.3-100%(91% in average) respectively. From the above results, it was confirmed that albendazole treatment is very effective against the adult worms of C. sinensis, however it is less effective on larvae and juvenile worms.

자루이형흡충(Stictodora fuscatum)의 표면 미세구조

손운목,Sohn, Woon-Mok Korean Society of Electron Microscopy 2000 Applied microscopy Vol.30 No.2

Present study was performed to observe the surface ultrastructures of Stictadora furcatum (Trematoda: Heterophyidae). Adult worms were recovered from the cat experimentally infected with metacercariae, and were prepared for scanning electron microscopy. The body was leaf-like and ventrally concave. The oral sucker armed with prominent tegumental spines on the inner surface, and it's lips were obscure. Sensory papillae (type I) in single or grouped forms symmetrically arranged around the oral sucker. The ventrogenital opening retained protruding gonotyl spines and sperms discharged from the genital pore . The body surface was covered with scale-like multipointed tegumental spines. The density and digitated point of spines were gradually decreased toward the posterior end of the body. The digitated points of spines on the anterior portion were 9-12, on the middle were 7-8 and 5-6, and on the posterior were 2-3 and peg-like. Although the tegumental ultrastructure of S. fuscatum was generally similar to those of other heterophyid flukes , the oral sucker with tegumental spines on the inner surface and without obvious lips, and the ventrogenital opening with protruding gonotyl spines were suggested to be the characteristic features.

Anisakis 및 Contracaecum 幼蟲 횡단면의 內部 構造

손운목,이순형 인제대학교 1989 仁濟醫學 Vol.10 No.4

Anisakis trpe I 幼蟲과 Contracaecum tupe A 및 tupe D' 幼蟲을 연속 절단하여 횡단면을 비교 관찰함으로써 이들에 대한 形態學的인 지견과 임상적인 진단에 필요한 기본 자료를 마련하고자 하였다. 蟲體 절단면에서 Anisakis type I 幼蟲은 비교적 큰 蟲體 斷面과 Y 또는 T형의 lateral chord 및 소세지 모양의 renette cell 등으로 쉽게 구분되나, Contracaecum 幼蟲은 단편적인 절단면만으로는 타입간의 감별이 용이치 않았다. This study was attempted to observe the cross sectional morphology of anisakid larvae and to make differential criteria in the diagnosis of anisakiasis. Anisakid larvae were collected from the viscera of Pseudosciaena manchurica and Anago anago which had been purchased from Noryangjin fisheries market. They were fixed in 70℃ neutral buffered formalin, serially cross-sectioned and stained with hematoxylin and eosin. Worm sections were observed and measured under the light microscope. Followings are summarized results. 1.Maximum diameter of Anisakis type I larvae was 280-340 (313 in average)μm. Esophagus and ventriculus were sectioned in triad by their luman. The shape of lateral chords in anterior part of ventriculo-intestinal junction was slender Y form. However, at its posterior portion was T form. Renette cell was small and plump at esophagus level, pressed at ventricular level and sausage-formed at anterior portion of intestinal level. 2.Maximum diameter of Contracaecum type A larvae was 230-280 (269 in average) μm. Lateral chord were not regular form. Genital anlage and lateral alae were characteristically observed in the sections of posterior body. However, renette cell was not observed. 3.Maximum diameter of Contracaecum type D' larvae was 184-230 (211 in average) μm. Lateral chord was butterfly or W-shaped. Genital anlage was observed wth posterior part of intestine. However, renette cell was not observed. From the above results, it is concluded that Anisakis type I larva is easily distinguished from Contracaecum spp. larvae in the sectioned specimens. However, it is difficult to differentiate the types of Contracaecum larvae in the sectioned specimens.

Metagonimus Takahashii와 Heterophyes Nocens의 혼합인체감염 1례

손운목,고원규,조규일 인제대학교 1992 仁濟醫學 Vol.13 No.2

1991년 8원 16일 부산백병원 종합검진센타를 찾은 27세 남자의 대변에서 異形吸蟲類의 충란이 검출되었다. 한달후 parziquantel과 황산 마그네슘을 투여하여 치료하였던 바, 587 마리의 M. takahashii와 8 마리의 H. nocens가 검출되었다. 이에 생물학적, 역학적 性狀이 다른 두 이형흡충류의 혼합인체감염 1례를 보고함과 아울러 분류학적으로 이견이 많은 M. takahashii의 형태학적 특징을 재기술하였다. A case of natural human infection by Metagonimus takahashii and Heterophyes nocens was confirmed by collection of adult warms after praziquatel treatment on August, 1991, and in order to clarify the species identity, the morphological characteristics of M. takahashii were rediscribed. The patient was a 27-year old male residing in Hadong-gun, Kyungnam Province, Korea. He had the clinical complaints of fatigue, indigestion and arrhythmia, and said to have eaten raw flesh of several kinds of marine and/or freshwater fish. Praziquantel in single dose 10mg/kg was given followed by purgation with magnesium salt, and 587 M.takahashii and 8 H. nocens were collected from the diarrheal stools. M. takahashii were dorsoventrally flattened, 0.998×0.573mm in average size, and they had the following morphological characteristics : no posteriormost location of right testes ; separated left testis from the right one ; distribution of uterine tubules over the left testis and intertesticular junction ; distribution of the vitellaria passing through the posteriormost portion of the body ; larger size of the eggs. H. nocens were also dorsoventrally flattened, 0.924×0.543mm in average size, and they had a well-developed ventral sucker and a genital sucker bearing the 54 chitinous rodlets. From the above results, a case of human infection by M. takahashii and H. nocens was identified. This study may be the 32nd human case of H. nocens infection in the literature in Korea.

Soboliphyme baturini (Nematoda: Soboliphymatidae) Recovered from Stomach of Asian Badger, Meles leucurus, in Geochang-gun, Gyeongsangnam-do, Korea

손운목,나병국 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5

We are going to describe the female soboliphymid nematodes, which were recovered from the stomach of a Asian badger, Meles leucurus (Mammalia: Mustelidae), in Geochang-gun, Gyeongsangnam-do, Korea. In February 1998, we found 2 peculiar nematodes with a cup-like organ in the anterior end from the stomach of badger. Recovered worms were fixed with 10% formalin, cleared in glycerin-alcohol solution and observed under a light microscope with a micrometer. They were 34.46 (33.43-35.50) mm long by 2.13 mm at maximum width. Cephalic sucker cup-like, 3.34 (3.13-3.55) mm wide, 2.40 (2.25-2.55) mm long, with the oral aperture and meridionally striated on the buccal capsule. Oral aperture 2.38 mm in diameter. Circumoral membrane 0.41 (0.38-0.45) mm wide. Esophagus muscular, 4.81 (4.50-5.00) mm long by 0.80 (0.78-0.83) mm at maximum width. Vulva situated at 3.13 mm ventro-anterior level from the esophago-intestinal junction. Vagina anteriad, 3.38 mm long, making a canal from the uterus to the vulva opening. Uterus single, large. Tail 0.35 (0.33-0.38) mm long. Intrauterine eggs long elliptical, 0.058-0.065 (0.062) mm long and 0.030-0.033 (0.031) mm wide. Based on the some morphological characters and host-specificity, our specimens are nearly identical with S. baturini. Therefore, the present report describes S. baturini for the first time in Korea.

흰쥐의 간흡충 감염에 대한 국산 프라지콴텔의 살충 및 예방효과

손운목,이순형 인제대학교 1993 仁濟醫學 Vol.14 No.3

흰쥐의 간흡충 감염에 대한 국산 프라지콴텔(DistocideR)의 살충 및 예방효과를 관찰하고자 하였다. 간흡충의 피낭유충은 자연감염 참붕어로부터 분리 수집한 것이며 흰쥐는 실험실에서 통상적으로 키운 200g 내외의 Sprague-Dawley계이다. In virto효과를 관찰하기 위하여 피낭유충을 0.1, 1.0, 10 및 100㎍/ml 농도의 프라지콸텔 용액에 넣어 1시간 동안 보존한 후 흰쥐에 경구감염시켰고, in vivo 효과를 관찰하기 위하여 감염 6시간 전, 3시간 전, 1시간 전 및 후에 5~9마리의 흰쥐에 25, 50 및 100mg/kg 프라지콸텔을 경구투여하였으며 감염 6주 후에 충체를 회수하여 충체 회수율로 약효를 판정하였다. In virtro에서 0.1㎍/ml 및 1.0㎍/ml 농도의 프라지콴텔 용액에 접촉시켰던 실험군에서는 살충효과가 인정되지 않았지만 10㎍/ml 및 100㎍/ml 농도에 접촉시켰던 실험군에서는 평균 16% 및 22%의 충체가 회수되어 대조군에 비해 유의하게 낮은 회수율을 나타내었다. In vivo 실험에서는 감염 6시간 전에 25㎍/kg 의 약제를 투여한 실험군에서 30%의 충체가 회수되어 57.1%의 치유율을 나타내었을 뿐 다른 모든 실험군에서 0∼1.2%의 충체가 회수되어 98.3∼100%의 치유율을 나타내었다. 이상의 결과로 프라지콴텔이 간흡충의 성충은 물론 피낭유층에 대해서도 매우 우수한 살충효과가 있음을 확인하였고 간흡충 감염의 예방약제로도 유용할 것으로 판단되었다. Ⅰ.머리말 Ⅱ.재료 및 방법 1.간흡충 피낭유충 획득 2.사용된 약제 3.실험동물 4.In vitro 실험 5.In vivo 실험 6.약효 판정 Ⅲ.결과 1.In vitro 실험 2.In vivo 실험 3.치유율 Ⅳ.고찰 Ⅴ.맺는말

만손열두조충의 발육단계별 표피 미세구조

손운목,이진하,Sohn, Woon-Mok,Lee, Jin-Ha 한국현미경학회 2005 Applied microscopy Vol.35 No.1

실험실내 생활사를 통하여 획득한 만손열두조충의 각 발육단계별 유충 및 성충의 표피미세구조를 주사 및 투과전자현미경으로 관찰하고자 하였다. 주사전자현미경 관찰에서 섬모유충은 타원형이었고 체표면에 직경이 약 $0.2{\mu}m$인 섬모, 작은 결절 및 좁쌀모양의 돌기 등이 밀생하고 있었으며 직경 약 $0.2{\mu}m$인 구멍이 드물게 분포하였다. 원미충의 전단에는 가시모양 미세모가 밀생하였고 체부위 체표면에는 끝이 약간 구부러진 긴 쐐기형 미세모가 밀생하였으며 꼬리혹에는 둔단의 짧은 미세모가 분포하였다. 실험감염 올챙이에서 감염 후 3일에 회수한 충미충은 성숙한 원미충과 형태가 유사하였고 전단홈에는 억센 가시모양의 미세모가 밀생해 있었다. 감염 5일된 충미충의 전단홈에는 3일된 충체에서 관찰되었던 가시모양 미세모는 없었고 체부위에서 관찰되는 것과 동일한 미세모가 분포하였다. 성숙한 충미충의 체표면에는 원추형의 미세모가 밀생해 있었는데 전반부에 분포하는 것이 후반부의 것보다 약간 억센 모양이었다. 성충의 두절에는 긴 섬모상의 미세모와 짧고 억센 모양의 미세모가 혼재되어 있었으며 섬유상 미세모의 밀도는 두절의 중간부위보다 전반부에서 높았다. 경절과 미성숙 편절에는 짧고 억센 원추형의 미세모가 밀생해 있었다. 투과전자현미경 관찰에서 섬모유충은 배막과 육구유충이 명백히 나누어져 있었고 배막에는 무수히 많은 섬모와 글리코겐 입자, 사립체 및 지질과립 등이 분포하였다. 섬모는 9쌍의 미세소관과 2개의 중심복합체로 구성되어 있었고 육구유충은 얇고 표면이 매끈한 표피로 덮여 있었으며 다핵성 구조이었다. 원미충 및 충미충의 표피는 송곳 모양의 미세모가 포함되어 있는 원형질막과 합포체, 기저층 및 근육층으로 이루어져 있었고 합포체에는 수 많은 원반모양체와 공포 및 사립체 등이 분포하였다. 실질층에서는 배설관과 표피세포가 관찰되었으며 표피세포는 핵 주위에 무수히 많은 과립형질내세망과 분비과립 등을 내포하고 있었다. 이상의 결과로 만손열두조충의 표피미세구조가 발육단계에 따라 변화하면서 특징적인 소견을 나타냄을 확인하였다. Present study was performed to observe the tegumental ultrastructures by the developmental stages which derived from the experimental life cycle of Spirometra erinacei in laboratory conditions. In SEM view, coracidium was spherical in shape with numerous cilia, and its surface was covered with long cilia, tuberclelike projections with millet-like processes, and small holes. The body surface of procercoid was covered with numerous pointed microtriches except that of frontal pit with stout spine-like ones. However that of cercomer was covered with somewhat sparse blunt-tiped microtriches. Plerocercoids of 3 days old resembled the mature procercoid in shape, and their frontal pits were covered with numerous stout spine-like microtriches. However frontal pit and body surface in more than 5 days old ones were covered with conoid microtriches. On the surface of adult scolex, hairly long filamentous and stout short microtriches were mixedly distributed. Filamentous microtriches were more densely distributed in the anterior portion than in the posterior of scolex. The neck and immature proglottid were covered with only stout short conoid microtriches. In TEM view of coracidia, embryophore and oncosphere were obviously distinguished. The embryophore contained numerous glycogen particles, mitochondria and lipid granules. The cilia on the surface of embryophore rooted in the coracidial sheath, and consisted of 9 pairs of microtubules and 2 core complex. The oncosphere was covered with a thin and unarmed tegument, and was multi-nucleated. The protoplasmic layer of procercoid and plerocercoid consisted of disc-shaped bodies, vacuoles and mitochondria. Their tegumental cells commonly retained a nucleus, granular endoplasmic reticulums and secretory granules. The protoplasmic layer of plerocercoid was more compacted than that of procercoid. From the above results, it was confirmed that the tegumental ultrastructures are something different according to the developmental stages of S. erinacei.

Echinoparyphium recurvatum의 레디아, 유미유충 및 피낭유충의 표피미세구조

손운목,고원규 인제대학교 1993 仁濟醫學 Vol.14 No.4

투과전자현미경을 이용하여 Echinoparyphium recurvatum의 레디아, 유미유충 및 피낭유충의 표피미세구조를 관찰하고자 하였다. 자연감염된 물달팽이로부터 획득한 유충들을 통상적인 시료제작 과정을 거친 후 JEOL 1200EX-II 투과전자현미경으로 관찰하였다. 레디아의 바깥 표피면에는 원형질층에서 돌출한 미세융모들이 분포하였고, 원형질층의 아래에는 구불구불한 형태의 기저층이 위치하였으며, 실질층에는 막성지질과립, 화염세포 및 표피세포 등이 분포하였다. 레디아 내에서 발육 중인 유미유충의 원형질층에는 전자밀도가 낮은 과립과 길죽길죽한 사립체가 분포하였으며 발육함에 따라 바깥 표피면의 융기 및 굴곡이 심화되었다. 성숙 유미유충의 원형질층에서는 다양한 형태의 과립과 피극 및 감각유두 등이 관찰되었다. 피낭유충의 낭벽은 섬유성 물질로 이루어져 있었으며, 원형질층은 유미유충 시기에 비하여 훨씬 조밀한 구조를 나타내었다. E. recurvatum 유충의 표피미세구조가 기본적으로는 서로 유사하나 발육단계에 따라 그에 상응하는 독특한 구조로 변화하였다. An transmission electron microscopic study was performed to observe the tegumental ultrastructures of the redia, cercaria and metacercaria of Echinoparyphium recurvatum. Each stage of the larval worms were obtained from the naturally infected fresh water snail, Radix auricularia coreana. They were prepared for transmission electron microscopic observations and observed by a JEOL 1200EX-II transmission electron microscope. The findings were summarized as followed. 1.The entire surface of the redial tegument was covered with microvilli, 0.45×0.4 μm in average size, which were not branched usually. The highly convoluted basement layer was 0.2μm wide or so. One kind of electron-dense secretory granules, flame cells and tegumental cells were observed in the parenchymal layer. 2.The cytoplasmic layer of a developing cercaria in redia was about 1.3μm wide and contained numerous electron-luscent secretory granules and mitochondria. 3.The cytoplasmic layer of a mature cercaria was about 2.4μm wide and contained 4 types of secretory granules, tegumental spines and ciliated sensory vesicle in its matrix. 4.The cyst wall of a metacercaria was consisted of a fibrous chitin substance. Numerous polyribosomes and small elliptical secretory granules were observed in the parenchymal layer of metacercaria. From the above findings, it is concluded that some characteristic features by the layer stage of E. recurvatum are obtained and they are helpful in the ultrastructural studies of other larval trematodes.

Matorchis orientalis 表皮 微細構造의 發育 段階別 變化

손운목,홍성종,이순형 인제대학교 1991 仁濟醫學 Vol.12 No.4

Metorchis orientalis의 發育 段階別 表皮 徵細構造를 주사전자현미경을 사용하여 관찰하였다. 충체가 성장 발육함에 따라 腹吸盤이 蟲澧 前方 1/3지점까지 올라와 位置하였고, 排泄孔도 충체 後端에서 腹側 前方으로 많이 올라와서 開ㅁ하였다. 배설공 주위에는 皮棘 및 感覺乳頭가 없고 多孔性의 原形質突起로 이루어진 독특한 구조의 표피가 分布하였다. 口吸盤 및 腹吸難의 口脣에는 각자 10개 및 6개의 제 Ⅱ형 감각유두가 일정한 간격으로 배열되어 있었다. 2일, 5일 및 7일된 幼苦蟲의 앞쪽 및 중간 부위 표피에는 손가락모양의 3∼6분지 피극이, 뒷쪽 부위에서는 송곳모양의 單棘이 분포하였고 2주 및 3주된 성충에서는 前, 中, 後部의 표피에 각각 9∼11分枝, 7∼10分技 밑 6∼7分技의 피극이 분포하였다. 유약충에서 자갈 모양이던 원형질돌기가 성충에서는 융단 모양이었으며 腹側面과 背側面에서 다소 配列樣相이 달랐지만 충체의 左右 外側에 1개 또는 3∼8개(grouped)의 제Ⅰ형 감각유두가 대칭적으로 배열되어 있었다. 감염 후 3주된 충체에서 표피의 破壞 및 脫落 現象이 관찰되었다. 이상의 결과로 M. orientalis의 표피는 감염후 2주까지 원형질돌기 밑 피극의 分化가 계속되고 3주 이상된 충체에서는 표피의 파괴 및 탈락이 일어남을 알 수 있었다. This study was performed to observe the tegumental ultrastructures of Metorchis orientalis according to the developmental stages. The worms were obtained from the experimentally infected chicks at 2, 5, 7, 14 and 21 days post infection. They were prepared for scanning electron microscopic observation and observed by ISI Korea DS-130C SEM at an accelerating voltage of 10 Kv. All worms were elongated and ventrally curved. Their ventral suckers were located in anterior 3/5∼1/3 level. The oral and ventral suckers had 10 and 6 type II sensory papillae respectively in a circular band on their lips. The ventro-anterior and ventro-middle surface of juvenile worms (2-day, 5-day and 7-day old) were regularly arranged with the spines of which tips diverged into 3 to 6 points, and their ventro-posterior surface were arranged with the peg-like single-tipped spines. The ventral surface of adult worms (2-week and 3-week old) was armed with many scale-like spines; 9∼11 pointed ones on anterior body, 7∼10 pointed on middle body and 6∼7 pointed on posterior body. The tegument spines on the dorsal surface were similar to those of ventral side in their shape and distribution. The tegument of juvenile worms was covered with cobblestone-like cytoplasmic processes, however they were changed into velvety texture in that of adults. The ciliated type I sensory papillae appeared biilaterally on the tegument of both side, and they were distributed on anterior tegument to ventral sucker level in group of 3∼8 ones. Destruction and shedding of tegument suggesing worm death and discharge were observed in 3-week old worms. From the above results, it is considered that the tegument of M. orientalis continued to differentiate until 2 weeks after infection, and it was shedded from the body of 3-week old worms.

Anisakis type I 幼蟲에 의한 人體感染 2例

손운목,고원규,설상영,정정명 인제대학교 1991 仁濟醫學 Vol.12 No.3

91년 5월 17일에 上腹部痛을 主訴로 本院을 내원한 2명의 患者에 대하여 胃 內視鏡檢査를 시행하였던 바, 胃 粘膜에 길이로 묻혀 있는 Anisakis 幼蟲을 1마리씩 檢出하였다. 胃 粘膜이 충체를 중심으로 肥厚되어 있었고 肉芽腫性 病巢가 형성되어 있었다. 검출된 충체는 둘다 Anisakis type I 幼蟲이었다. Two cases of gastric anisakiasis caused by Anisakis type I larva were reported. The patients, residing in Pusan, were 66-year-old male and 36-year-old female. They chiefly complained of epigastric pain and recalled they had eaten raw marine fish such as sea eel and bream. Two whitish round worms were found in the hypertrophic and granulomatous mucosa of stomach wall on gastroscopic examinations, and were classified as Anisakis type I larva.