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DBP스폰지에서 BMP-2의 효과확인 및 섬유륜 조직재생
최진희 ( Jin Hee Choi ),장지욱 ( Ji Wook Jang ),김순희 ( Soon Hee Kim ),홍희경 ( Hee Kyung Hong ),민병현 ( Byung Hyun Min ),손영숙 ( Youngsook Son ),이종문 ( John M Rhee ),강길선 ( Gilson Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Demineralized bone particle(DBP) that affects to cell proliferation and differentiation has been used as biomaterials. In this study, we evaluated 3-dimensional DBP sponge and Collagen sponge on proliferation and phenotype maintenance of annulus fibrosus cells. DBP sponge were prepared by freeze-drying method after addition 2 wt% DBP solution. sponge was crosslinked with 1-ethyl-(3-3-dimethyl aminopropyl) carbodiimide hydrochloride(EDC) solution with 50 mM concentration for 24 hrs and lyophilized. We seeded cells in DBP sponge and Collagen sponge. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. DBP sponge and Collagen sponge were characterized by scanning electron microscopy(SEM). Reverse transcription polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression for type I collagen, type II collagen, aggrecan and osteonectin and glycosaminoglycan(GAG) quantity from annulus fibrosus cells in sponges. In MTT assay result, DBP sponges were higher cell viability. In SEM observation, we observed that DBP sponge has uniform porosity. In addition, annulus fibrosus stronly expressed their specific mRNA. and produced well sGAG in DBP sponge. This result indicates that DBP sponge is useful for intervertebral disc regeneration.
In vitro상에서 연골재생을 위한 케라틴/PLGA 지지체의 효과
홍현혜 ( Hyun Hye Hong ),김순희 ( Soon Hee Kim ),오아영 ( A Young Oh ),전나리 ( Na Ri Jeon ),정수현 ( Su Hyun Jung ),( Sang Jin Lee ),( Mark Van Dyke ),( James J. Yoo ),손영숙 ( Youngsook Son ),이종문 ( John M. Rhee ),강길선 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Synthetic and naturally derived biodegradable polymers have been widely used to construct scaffolds for cartilage tissue engineering. We developed the keratin loaded poly(L-lactide-co-glycolide)(PLGA) scaffolds(keratin/ PLGA) to utilize as highly functional scaffolds for tissue engineering by improving hydrophobicity and cell compatibility of the polymer scaffolds. Keratin as natural protein is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin/PLGA scaffolds were prepared by casting/salt leaching method. Cell proliferation activity was measured via MTT assay. Scaffold mechanical strength, histology, gene expression, sulphated- glycosaminoglycan(sGAG) and collagen contents analyses were performed to elucidate in vitro cartilage development and the deposition of cartilage-specific extracellular matrices. We concluded in vitro chondrogenesis of articular chondrocytes was improved in PLGA scaffolds containing keratin.
위암세포주에서 Recombinant Human Interferon-r와 Adriamycin의 투여순서가 항암효과에 미치는 영향
홍원선,손영숙,김창민,강윤구,이춘택,김유철,임영혁,남현석,이진오,강태웅 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
Numerous previous studies, both in vitro and in vivo, have demonstrated that the cytotoxicity can be enhanced by the combination of chemotherapeutic agent and interferons(IFNs) in various types of cancer cells. We have previously reported that combined treatment of MKN-45, human gastric adenocarcinoma cells, with adriamycin(ADM) and recombinant human interferon-r(rh-IFN-r) increased in the cytotoxicity. In this study, the effects of combination timing of rh-IFN-r and ADM on the cytotoxicity against MKN-45 were investigated using MTT assay. MKN-45 was treated with rh-IFN-r and ADM in vitro on three schedules : Treat A ; rh-IFN-r and ADM were treated simultaneously, Treat B ; rh-IFN-r was treated 24 hours after the treatment with ADM, Treat C ; rh-IFN-r was treated for 72 hours and followed by the treatment with ADM. The survival of MKN -45 was inhibited by ADM dose-dependently. 102 and 103U/ml of rh-IFN-r significantly inhibited the survival of MKN-45(% survival : 35.1 ±-1.2% and 34.4 ±1.1% in Treat A and 42.5 ± 2.1% and 45.9-±2.5% in Treat C, respectively). However no difference in the survival was observed between 102 and 103U/ml of rh-IFN-r. Combined treatment with rh-IFN-r and ADM significantly augmented the cytotoxicity at low concentrations of ADM. Combined effects of rh-IFN-r and ADM were evaluated using IC30(,ag/ml) to ADM. IC30s of MKN-45 in Treat A, B and C at 102 U/ml of rh -IFN-r _ were 0.019 -?- 0.003, 0.045 :I:0.001 and 0.054 ± 0.012, respectively, while IC30 of MKN-45 treated with ADM alone was 0.052±0.004. IC30s of MKN-45 in ADM alone group, Treat A, Treat B and Treat C at 103U/ml of rh-IFN-r were 0.047 ±0.003, 0.004 -±0.001, 0.031 ±0.004 and 0.056 0.008, respectively. These results indicate IC30s of Treat A and B were significantly lower than those of ADM alone(p<0.05) and IC30s of Treat A was significantly lower than those of Treat B(p <0.01). IC30s of Treat C, however, were not different from those of ADM alone. From these results demonstrating that cytotoxic effects were increased by the combination of rh-IFN-r and ADM in the order, Treat A > Treat B> Treat C, it can be concluded that the simultaneous administration of rh-IFN-r and ADM may be the most effective method to combine these two therapeutic modalties.