병원성이 강한 Plasmodiophora brassicae PC91214 (race 4) 균주에 대한 배추와 무 품종의 저항성 평가

소재우,한경숙,이성찬,이중섭 경상대학교 농업생명과학연구원 2013 농업생명과학연구 Vol.47 No.3

The research was conducted in order to investigate the pathogenicity of Plasmodiophora brassicae strains isolated from main Chinese cabbage producing areas in Pyeongchang, Korea, and the resistant between cultivars of Chinese cabbage and radish. From the race examination using 4 Williams differential hosts, the disease incidence rates of retained strains (RSs) were 64.1~90.6%, while the newly isolated strains (NISs) showed the disease incidence rates at 98.4~100% and the disease severity at 4.2~4.6, meaning that NISs were race 4 have with higher pathogenicity than RSs. The disease incidence rates and the disease severity of RSs were Yeokkwang 100.0% and 3.1, Maeryeok 100% and 3.9, Norang Bom 100% and 4.8, Chungwang 100% and 3.9, Jeongsang 100% and 4.8, and Cheongdaebom 35.9% and 1.0. When it comes to NISs. However all Chinese cabbage cultivars showed that the disease incidence rate was 100% and the disease severity was 3.7–5.0. The disease incidence rates and disease severity on white radishes were 60.9% and 1.5 for Gangseongmu, 98.4% and 3.2 for Gwandongyeoreummu, 100% and 4.1 for Baekbongmu, and 100% and 4.7 for Cheongdaebommu. These results indciated that the virulence of NISs were higher than that of RSs. 본 연구는 새로운 뿌리 혹병균 균주의 병원성 차이와 배추와 무의 저항성 차이를 구명하고자 수행하였다. Williams 판별 기주 4종을 이용한 접종 균주들의 race검정에서 기보유 균주는 발병률 64.1-90.6%이었고, PC91214 균주는 발병률 98.4-100%, 발병도 4.2-4.6를 보여 두 균주 모두 race 4이지만 PC91214 균주가 더 높은 병원성을 보이는 것으로 나타났다. 각 균주별 배추와 무의 시판 품종의 저항성을 조사하였다. 기보유 균주 접종 처리에서 발병률과 발병도를 조사하였는데, 역광 100.0%와 3.1, 매력 100%와 3.9, 노랑봄 100%와 4.8, 춘광 100%와 3.9, 정상 100%와 4.8을 보여 총 5품종에서 강하게 발병하였다. 반면 무는 청대봄무만 발병률 35.9%와 발병도1.0의 극약으로 발병하였고 공시한 무 품종 대부분이 기보유 균주에 대하여 저항성인 것으로 확인되었다. 하지만 PC91214 균주 접종 처리에서 모든 배추에서 발병률100.0%와 발병도 3.7–5.0을 보였고, 무의 발병률과 발병도는 강성무 60.9%와 1.5, 관동여름무 98.4%와 3.2, 백봉무 100%와 4.1, 청대봄무 100%와 4.7을 보여 PC91214 균주가 기보유 균주보다 더 강한 병원성과 시판 품종간 차이를 극명하게 나타내었다. 이상의 결과로부터 고랭지 주산지인 평창군에서 기존의 Plasmodiophora brassicae보다 더 강한 병원성을 가진 PC91214 균주가 확인되었다. 또한 배추는 CR 저항성 품종을 포함한 모든 품종이 이병되어 PC91214 균주에 대한 저항성 품종 육성이 시급한 것으로 나타났다. 무는 배추와 달리 PC91214 균주에 대하여 품종간 저항성 수준이 극명한 것으로 나타나 배추와 다른 유전자 및 유전 양식을 가지는 것으로 나타났다.

배추 산지 토양의 뿌리혹병균 오염

소재우,한경숙,이성찬,이중섭 한국식물병리학회 2013 식물병연구 Vol.19 No.3

본 연구는 가을 배추 산지로 알려진 지역의 토양내 P. brassicae 의 오염 조사를 통하여 배추 뿌리혹병 방제의기초 기술을 확립하고자 수행하였다. 배추과 작물에 뿌리혹을 유발하는 P. brassicae를 검출하기 위한 PCR primer를 개발하였다. PbbtgF761과 PbbtgR961의 primer 세트는P. brassicae에 특이적인 245 bp 크기의 밴드를 증폭하였다. 가을 배추 재배지로 알려진 해남군은 33곳 중 10곳,영암군 및 영광군은 13곳 중 5곳, 고창군은 6곳 중 1곳,홍성군은 12곳 중 2곳, 당진시는 17곳 중 5곳이 P. brassicae에 오염된 것으로 나타났다. 토양내 P. brassicae에 의한오염률 조사에서 해남군 30.3%, 영암군 및 영광군 38.5%,고창군 16.7%, 홍성군 16.7%, 당진시 29.4%로 나타났다. 배추 뿌리혹이 육안으로 확인된 6곳은 PCR 검정에서 토양내 P. brassicae를 100% 확인할 수 있었다. 따라서 토양내 P. brassicae의 오염을 PCR 진단을 통해 화학적 방제여부를 결정하는 지표로 활용할 수 있을 것이다. This research was performed to establish basic technology for Chinese cabbage clubroot chemical control by investigating the soil contamination of Plasmodiophora brassicae in major producing regions of fall Chinese cabbage. PCR primers were developed to detect P. brassicae, a causal agent of Chinese cabbage club-root that generally occurs in Cruciferae family. A primer set, PbbtgF761 and PbbtgR961, specifically amplified a 245 bp fragment from P. brassicae only. At places well known for fall Chinese cabbage, 10 out of 33 in Haenam-gun, 5 out of 13 in Yeongam-gun and Yeonggwang-gun, 1 out of 6 in Gochang-gun, 2 out of 12 in Hongseong-gun, and 5 out of 17 in Dangjin-si resulted positive for P. brassicae contamination. The results show that the soil contamination rate of P. brassicae was 30.3% in Haenam-gun, 38.5% in Yeongam-gun and Yeonggwang-gun, 16.7% in Gochang-gun, 16.7% in Hongseong-gun, and 29.4% in Dangjin-si. The six places where Chinese cabbage clubroot was visible by naked eye were 100% confirmed by the PCR test of the P. brassicae contaminated soil. Thus, simple PCR test may be utilized as an index to decide on chemical control of P. brassicae.

파인 오일과 수산화동 혼합물에 의한 고추 세균점무늬병과 탄저병의 방제 효과

소재우,한경숙,이성찬,이중섭,박종한 한국식물병리학회 2014 식물병연구 Vol.20 No.3

This research was performed to examine the protective activities of the mixtures of pine oil and copperhydroxide against bacterial spot and anthracnose on pepper plants. As for bacterial spot, the treatment ofpine oil alone displayed high disease incidence (59.6%) and low protective effect (28.9%). In comparison, thetreatments of mixtures and copper hydroxide alone showed protective activities of 66.8-76.1%. The mixtureof pine oil and copper hydroxide (4:1) suppressed the most effectively bacterial spot on pepper. On the otherhand, the mixture of pine oil and copper hydroxide (4:1) also showed the strongest protective effect againstpepper anthracnose among the 4 treatments tested; its disease incidence and disease control value were49.8% and 41.7%, respectively. The other treatments showed low protective activities with control values of7.4-17.1%. These results suggested that the mixture of pine oil and copper hydroxide (4:1) can be used for theenvironmental-friendly disease control of bacterial spot and anthracnose on pepper.

고추 역병균의 접종원에 따른 역병 저항성의 유전 양식

소재우,한경숙,이성찬,이중섭 한국식물병리학회 2012 식물병연구 Vol.18 No.4

The study aims to identify the pathogenicity of Phytophthora. capsici isolates in major pepper-producing areas in Korea and the inherit genetic pattern of phytophthora blight resistance by inocula. With five kinds of testing materials including ‘Kataguma (Sakata Korea)’ peppers, a disease-susceptible material, ‘#308’, a phytophthora blight resistance material, ‘CM334’, and their F1 and F2, respective isolates of P. capsici obtained from Icheon, Eumseong, Buan, Imsil and Yeongyang regions together with six kinds of peppers’inoculum including PA-159 (KACC No.40482) received from Korean Agricultural Culture Collection were used for inoculation. The disease-susceptible material ‘#308’, the resistant material ‘CM334’ and the nonsegregating generation of F1 represented 4.94−5.00, 1.00−1.07, and 1.01−1.08 phytophthora blight incidence respectively in the group comparison by isolate. This result means that the phytophthora blight resistance was clearly distinguished among testing materials in the group comparison by P. capsici isolate. Moreover, F2segregating generation showed 1.79−2.31 phytophthora blight incidence which turned out to be identical in the group comparison by the six isolates of P. capsici isolate and with similarity between both the resistant and susceptible materials. Thus, the result proved that using the six isolates of P. capsici tested as inocula was suitable to investigate the phytophthora blight resistance. When it comes to group comparison of F2segregation generation, however, isolates were divided with PA-159 isolate being the center: a group consisting of isolates from Icheon, Buan, and Imsil and a group consisting of Yeongyang and Eumseong isolates with higher pathogenicity. The expected segregation ratio of the phytophthora blight resistance in F2generation by isolate was analyzed. PA-159 isolate showed 3:1 or 9:3:3:1, indicating that one to two genes were involved. On the other hand, results also proved that there is an interaction of genes since both Eumseong and Yeongyang isolates showed a segregation ratio of 11:5 while the Icheon isolate represented 12:3:1.

한국 고추맛의 유전 분석

소재우,조치웅 경상대학교 농업생명과학연구원 2012 농업생명과학연구 Vol.46 No.6

This study was carried out to determine the genic values of taste of Korean hot pepper (Capsicum annuum L.) in practical genetic resources of using to breed them. The two breeding materials of pepper, ‘#1803’ (P1) of prefer tastes and ‘#1532’ (P2) of ordinary taste, and their F1 and F2 generations were used in this study. By using partitioning method it was possible to estimate, from the F2 generation, the number of effective factor pairs differentiating the two parents. There were found to be differentiated by two effective factor pairs. In practical genetic resource of using to breed the Korean hot pepper, the heritance of pepper tastes showed that the F1 was better than excellent parent by reason of over‐dominant, but some F2 was better than both parent by transgressive segregation. As the result, the magnitude of genic effects of A-a gene in pepper tastes was 0.36, and B-b gene was 0.64. The tastes of Korean hot pepper showed a complex inheritance by interaction effect on the two non‐allelic factors of 0.94 and secondary effect of 2.86 at the most. 본 시험은 한국 고추 육종에 이용되는 교배친의 고추맛에 관여하는 유전자의 유전가를 구명하기 위해 수행하였다. 고추맛과 원예적 특성이 상이한 ‘#1803’(P1)과 ‘#1532’(P2) 두 교배친을 교배하여 이들의 F1, F2 세대를 본 시험의 공시 재료로 이용하였다. Partitioning method에 따라 고추맛은 A-a와 B-b 두 유효 유전자에 의하여 지배되는데 F1 세대는 우수 교배친 P1보다 더 우수한 초우성 현상과 F2 세대는 초월 분리가 일부 나타났다. 고추맛의 유효 유전자 효과는 A-a 유전자에 의해 0.36, B-b 유전자에 의해 0.64가 증가하였다. 한국 고추맛은 유효 유전자간에서 0.94의 간섭효과와 최대 2.86의 제 2효과가 관여하여 복합적인 유전양식을 나타내었다.

에스필과 수산화동 혼용에 의한 오이, 토마토, 고추 흰가루병의 친환경 방제 효과

소재우,한경숙,이성찬,이중섭,박종한 한국식물병리학회 2014 식물병연구 Vol.20 No.2

This research was performed to examine the effects of mixed espil and copper hydroxide for powdery mildew prevention on cucumber, tomato and pepper. On prevention effect for powdery mildew on cucumber, results revealed that mixed espil and copper hydroxide with a ratio of 4 : 1, disease incidence rate was 18.9% and prevention effect was 69.9%. Another treatment with a ratio of 8 : 1 showed an disease incidence rate of 18.1% and prevention effect of 71.1%, thus, showed great effectiveness. For powdery mildew on tomato, espil and copper hydroxide were mixed using the ratio 4 : 1, results showed an disease incidence rate of 12.4% and prevention effect of 85.3%. Treatment using the ratio of 8 : 1, results showed an disease incidence rate of 14.3% and prevention effect of 83.0%, thus, showed great effectiveness. For powdery mildew on redpepper, espil and copper hydroxide were mixed using the ratio of 4 : 1 with results showed disease incidence rate of 17.7% and prevention effect of 83.0%. From the results, this treatment is the most effective with the lowest attack rate and highest prevention effect. Deducing from the study, it was found out that using mixed espil and copper hydroxide using the ratios 4 : 1 or 8 : 1 are the most effective method for powdery mildew prevention. Mixed ratio of 4 : 1 or 8 : 1 was most effective for preventing powdery mildew on cucumber and tomato, while the espil and copper hydroxide ratio of 4 : 1 was the most effective method for powdery mildew prevention on pepper. This research was performed to examine the effects of mixed espil and copper hydroxide for powderymildew prevention on cucumber, tomato and pepper. On prevention effect for powdery mildew on cucumber,results revealed that mixed espil and copper hydroxide with a ratio of 4 : 1, disease incidence rate was18.9% and prevention effect was 69.9%. Another treatment with a ratio of 8 : 1 showed an disease incidencerate of 18.1% and prevention effect of 71.1%, thus, showed great effectiveness. For powdery mildew on tomato,espil and copper hydroxide were mixed using the ratio 4 : 1, results showed an disease incidence rate of12.4% and prevention effect of 85.3%. Treatment using the ratio of 8 : 1, results showed an disease incidencerate of 14.3% and prevention effect of 83.0%, thus, showed great effectiveness. For powdery mildew on redpepper,espil and copper hydroxide were mixed using the ratio of 4 : 1 with results showed disease incidencerate of 17.7% and prevention effect of 83.0%. From the results, this treatment is the most effective with thelowest attack rate and highest prevention effect. Deducing from the study, it was found out that using mixedespil and copper hydroxide using the ratios 4 : 1 or 8 : 1 are the most effective method for powdery mildewprevention. Mixed ratio of 4 : 1 or 8 : 1 was most effective for preventing powdery mildew on cucumber andtomato, while the espil and copper hydroxide ratio of 4 : 1 was the most effective method for powdery mildewprevention on pepper.

Performance Analysis of VoIP Services in MobileWiMAX Systems with a Hybrid ARQ Scheme

소재우 한국통신학회 2012 Journal of communications and networks Vol.14 No.5

This paper analyzes the performance of voice-over-Internet protocol (VoIP) services in terms of the system throughput,the packet delay, and the signaling overhead in a mobile WiMAX system with a hybrid automatic repeat request (HARQ)mechanism. Furthermore, a queueing analytical model is developed with due consideration of adaptive modulation and coding,the signaling overhead, and the retransmissions of erroneous packets. The arrival process is modeled as the sum of the arrival rate at the initial transmission queue and the retransmission queue, respectively. The service rate is calculated by taking the HARQ retransmissions into consideration. This paper also evaluates the performance of VoIP services in a mobile WiMAX system with and without persistent allocation; persistent allocation is a technique used to reduce the signaling overhead for connections with a periodic traffic pattern and a relatively fixed payload. As shown in the simulation results, the HARQ mechanism increases the system throughput as well as the signaling overhead and the packet delay.

고추 역병균의 접종원에 따른 역병 저항성의 유전 양식

소재우(Jaewoo Soh),한경숙(Kyungsook Han),이성찬(Sungchan Lee),이용범(Yongbeom Lee),김기홍(Kihong Kim) 한국원예학회 2012 한국원예학회 학술발표요지 Vol.2012 No.5

생육과 토양 개량 효과가 높은 새로운 순창 대두 근류균(Rhizobium tropici Sunchanng 180605)의 개발

소재우(Jae-Woo Soh),박혜원(Hey-Won Park),조민경(Min-Kyung Cho) 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.10

고추 탄저병 병원균에 따른 친환경 방제 효과

소재우(Jae-Woo Soh),한경숙(Kyung-Sook Han),이성찬(Seong-Chan Lee),박종한(Jong-Han Park),김경남(Kyoung-Nam Kim) 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.5