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간효소에 의해 대사된 양격산화탕의 저산소/재관류로부터 PCl2 세포 보호효과
소윤조(Yunjo Soh) 大韓藥學會 2005 약학회지 Vol.49 No.1
The protective effect of Yangguksanwha-tang (YST) against hypoxia-reperfusion insult was investigated in PC12 cells. To elucidate the mechanism of the protective effect of YST, cell viability the changes in activities of superoxide dismutase, glutathione peroxidase, catalase, caspase 3 and the production of malondialdehyde were observed after treating PC12 cells with YST which was metabolized by rat liver homogenate. Pretreatment of YST with liver homogenate appeared to increase its protective effect against hypoxia-reperfusion insult. The result showed that YST had the highest protective effect against hypoxia/reperfusion at the dose of 2㎍/ml in PC12 cells, probably by recovering the redox enzyme activities and MDA to control level.
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오가피(Eleutherococcus sessiliflorus )의 전연골성 ATDC5 세포의 분화 유도
스레스타사로즈쿠마 ( Saroj Kumar Shrestha ),송정빈 ( Jungbin Song ),이성현 ( Sung Hyun Lee ),이동헌 ( Donghun Lee ),김호철 ( Hocheol Kim ),소윤조 ( Yunjo Soh ) 대한본초학회 2022 大韓本草學會誌 Vol.37 No.1
Objectives : The process through which mesenchymal cells condense and differentiate into chondrocytes to form new bone is known as endochondral bone formation. Chondrogenic differentiation and hypertrophy are essential steps in bone formation and are influenced by various factors. The stem bark and root bark of Eleutherococcus sessiliflorus (ES) have been widely used to treat growth retardation and arthritis in traditional Korean Medicine. In this study, we aimed to investigate the possible role of the stem bark of ES in the stimulation of chondrogenic differentiation in clonal murine chondrogenic ATDC5 cells. Methods : In ATDC5 cells treated with ES extract, cell viability and extracellular matrix production were determined using CCK-8 assay and Alcian blue staining, respectively, and alkaline phosphatase activity was measured. We also examined mRNA and protein expression levels of genes related to chondrogenic expression in ATDC5 cells using reverse transcription-polymerase chain reaction and western blot analyses. Results : ES extract increased the accumulation of Alcian blue-stained cartilage nodules and alkaline phosphatase activity in ATDC5 cells. It increased the mRNA expressions of chondrogenic markers including bone sialoprotein (BSP), cartilage collagens, Runt-related transcription factor-2 (RUNX-2), osteocalcin (OCN), β-catenin, and bone morphogenetic protein-2 (BMP-2), as well as the protein expressions of β-catenin, RUNX-2, BMP-2, and alkaline phosphatase (ALP). Conclusion : Taken together, these results suggest that ES extract exhibits a chondromodulating activity and therefore may be a possible agent for the treatment of bone growth disorders.
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석회화 전처리가 양극산화 처리한 타이타늄 임플란트의 생체활성도에 미치는 영향
박소영(So-Young Park),박일송(Il-Song Park),오상훈(Sang-Hoon Oh),정회웅(Hoi-Wung Chung),소윤조(Yun-Jo Soh),김형섭(Hyung-Seop Kim) 대한치과재료학회 2011 대한치과재료학회지 Vol.38 No.4
This study was performed to improve the bioactivity of anodized nanotubular TiO₂ layer. The glycerol solution containing 1 wt% NH4F and 20 wt% deionized water was used as an electrolyte. Pulse signals with a potential of 20 V and current density of 20 ㎃/㎠ were applied for 60 minutes. Nanotubular TiO₂ layer was heat-treated at 500℃ for 2 hours and precalcified by soaking in Na₂HPO₄ solution at room temperature for 24 hours and then in saturated Ca(OH)₂ solution for 5 hours. The samples were divided into 4 groups: (1) pure Ti; (2) anodized Ti; (3) anodized and heat-treated; and (4) anodized, heat-treated and precalcified. The 36 implants were placed in both tibiae of 18 rats. After implantation periods of 2 and 4 weeks, the degree of osseointegration of implants were evaluated by micro CT analysis, western blotting and RT-PCR. Micro CT images revealed more dense appearance around screw part of implants in the anodized, heat-treated, and precalcified group than in other groups. Bone mineral density and trabecular density values exhibited coherent results with same oder. Collagen Type I and 2 as well as osteocalcin which are involved in bone remodeling also exhibited the highest expression in the anodized, heat-treated, & precalcified group than in other groups. In conclusion, anodized, heat-treated, and precalcified titanium implants significantly improved osseointegration ability in compared to others.
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마가목 및 현지초 추출물의 골손실 및 연골손상 억제효과
문은정(Moon, Eun-Jung),윤유석(Youn, You-Suk),최보윤(Choi, Bo-Yun),정현욱(Jeong, Hyun-Uk),박지호(Park, Ji-Ho),오명숙(Oh, Myung-Sook),소윤조(Soh, Yun-Jo),김선여(Kim, Sun-Yeou) 한국산학기술학회 2010 한국산학기술학회논문지 Vol.11 No.9
본 연구에서는 마가목 (SC), 현지초 추출물 (GT) 및 이들의 1:1 혼합물 시료 (MIX)가 골손실 및 연골손상 억제에 효과가 있는지 알아보기 위해, 각각의 시료를 조골세포주인 MG-63 세포, 파골세포로의 분화를 유도한 Raw264.7 세포와 연골세포로의 분화를 유도한 ATDC5 세포에 처리하여 세포분화 조절 정도를 확인하였다. 각 세포 의 분화 정도는 alkaline phosphatase (ALP) 활성 측정, tartrate-resistant acid phosphatase (TRAP) 염색법 및 alcian-blue 염색법으로 확인하였다. 이들 시료는 MG-63 세포에서 ALP 활성에는 영향을 미치지 않았으나, 마가목 추 출물 (SC) 및 마가목과 현지초 추출물의 혼합시료 (MIX)는 농도 의존적으로 파골세포의 분화를 억제하고 연골세포 의 분화를 촉진하는 것으로 나타났다. 이상의 결과를 종합하여 볼 때, 마가목과 현지초는 골손실과 연골 손상으로부 터 보호할 수 있는 중요한 천연물 소재임을 확인할 수 있었다. 나아가 이들 추출물의 작용기전 및 활성물질 구명에 대한 연구는 추후 더 진행되어야 할 것이다. This study was carried out to investigate the effect of Sorbus commixta (SC), Geranium thunbergii (GT) and their mixture (SC:GT=1:1, MIX) on inhibition of bone loss and chondral defect. To examine their activities, we measured the alkaline phosphatase (ALP) activity in human osteoblast-like MG-63 cells and performed tartrate-resistant acid phosphate (TRAP) staining in osteoclast differentiated from Raw264.7 cells. To investigate the influence on chondrocyte differentiation, we performed alcian-blue staining in chondrocyte differentiated from ATDC5 cells. All of SC, GT and MIX did not increase ALP activity in MG-63 cells. However, SC and mixture (SC:GT=1:1, MIX) significantly inhibited osteoclastic differentiation. And they also induced chondrocyte differentiation. These results suggest that SC and GT may have a potential for the treatment of bone loss and chondral defect by suppression of osteoclast differentiation and stimulation of chondrocyte differentiation. Therefore, clarification of their mechanisms and active components will be needed.
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