TNBS로 유도된 만성 염증성 대장염에 대한 대장유(大腸兪) 구진(灸津) 약침(藥鍼)의 효과

백대봉 ( Dae Bong Baek ),권오상 ( Oh Sang Kwon ),최원종 ( Won Jong Choi ),김재효 ( Jae Hyo Kim ),전희영 ( Hee Young Jeon ),김경식 ( Kyung Sik Kim ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2007 Korean Journal of Acupuncture Vol.24 No.3

Objectives: Inflammatory bowel disease (IBD) is a chronic relapsing inflammatory disease in the gastrointestinal tract. The sources and pathologic mechanisms of IBD are still unknown. Moreover conventional therapies for IBD are not always effective, and they often have serious side effects. The purpose of this study is to examine the effect of Moxi-tar herbal acupuncture in IBD affected mice. Methods: Mice were treated with 5 % 2, 4, 6-trinitrobenzenesulfonic acid (TNBS) on day 1 and day 7. To assume the preemptive effect and therapeutic effect, herbal acupuncture was practiced with Moxi-tar at BL25 (Daejangsu) on day 0, day 3, and day 6. The end of day in treatment with Moxi-tar herbal acupuncture, the mortality and the inflammatory factors of the colon were measured by the various methods. Results: TNBS induced high mortality but herbal acupuncture with Moxi-tar at BL25 suppressed the mortality caused by TNBS. TNBS induced infiltration of immune cells in all layers of the colon and increased myeloperoxygenase (MPO) activity, while the treatment with Moxi-tar herbal acupuncture at BL25 suppressed the infiltration of immune cells and the increase of MPO activity caused by TNBS to normal levels. Herbal acupuncture with Moxi-tar regulated NF-κB activity, which is an important factor for the pathogenesis of chronic colitis, and reduced the expressions of TNF-α, IL-1β, and ICAM-1 in the colons of TNBS treated mice. Furthermore herbal acupuncture suppressed macro- and micro- colonic damages caused by TNBS. Conclusions: This study demonstrates that herbal acupuncture with Moxi-tar at BL25 is a potential preemptive and/or therapeutic method targeting the chronic IBD.

흰쥐의 합곡부위(合谷部位)에 황련약침(黃連藥鍼)이 TNBS로 유발된 대장염(大腸炎)에 미치는 영향(影響)

도진우,안성훈,구성태,백대봉,김경식,손인철,Do, Jin-Woo,An, Sung-Hun,Koo, Sung-Tae,Baek, Tae-Bong,Kim, Kyung-Sik,Sohn, In-Chul 경락경혈학회 2004 대한침구의학회지 Vol.21 No.2

Objectives : Ulcerative colitis and Crohn's diseases are chronic inflammatory disease of the gastrointestinal tract identified mainly for diarrhea, stomachache, hemafecia. The source and pathologic mechanism about ulcerative colitis and Crohn's disease are still unknown but stress, infection, a genetic factor, environmental factors etc. may be the cause of chronic inflammatory disease of the gastrointestinal tract recently. Methods : Because ulcerative colitis and Crohn's disease is recognized as Ha-ri(下痢) or Jang-Byok(腸?) in Oriental Medicine, Rhizoma Coptidis Herbal acupunctures were injected in hapgok $(LI_4)$ which is used to treat intestinal disease in clinic. All animals were subjected to the injection of saline $(300\;{\mu}l,\;500\;{\mu}l)$ for a study control and TNBS $(300\;{\mu}l,\;500\;{\mu}l)$ into the lumen of the colon, 8cm proximal to the anus through the intestine. Rhizoma Coptidis Herbal (20 mg/ml, 0.4 ml) acupuncture solution was injected to the $LI_4$ (both hands) at the secondary injection time of TNBS in rats. And body weight, RBC count, WBC count, total protein, Paw edema rate, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels were observed to study the effects of Rhizoma Coptidis Herbal acupuncture in hapgok $(LI_4)$. Results : In results, Rhizoma Coptidis Herbal acupuncture in hapgok $(LI_4)$ on TNBS-induced colitis reduced the rate of weight loss but had no effect on RBC and WBC count. And it reduced total protein concentration, paw edema, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels. Conclusion : Data suggest that injection of Rhizoma Coptidis herbal acupuncture solution into hapgok has significant therapeutic effect on the colitis.

구진의 H2O2로 유발된 뇌신경세포 상해에 대한 보호 기전 연구

안성훈 ( Seoung Hoon Ahn ),안영남 ( Young Nam Ahn ),백대봉 ( Dae Bong Baek ),송문영 ( Moon Young Song ),김경식 ( Kyoung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.2

Objective: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased NO and iNOS generation in C6-glioma and RAW 264.7 cells in our Lab. The purpose of this research was to investigate the protect reaction on cell injury induced by the H2O2 in C6-glioma cells. Methods: C6-glioma cells were cultured in RPMI 1640 with FBS 10% in CO2 incubator. To study the protective effects of moxi-tar, we observed cell viability, DPPH activity, SOD activity, catalase activity and cell morphology after injury with H202. Results: Moxi-tar increased cell viability about twice as much as that of being injury by H2O2 (moxi-tar 40 μg/ml, H2O2 500 μM). And the results of free radical scavenger activity (80 μg/ml: 78.91±4.4%), SOD activity and catalase activity (80 μg/ml: 21.6 unit/mg protein) were increased by moxi-tar as dose-dependent manner. Conclusion: we concluded that the effects of moxibustion which is played important role in Oriental medicine, might be free radical scavenger effects induced by moxi-tar.

흰쥐의 합곡부위(合谷部位)에 황련약침(黃連藥鍼)이 TNBS로 유발된 대장염(大腸炎)에 미치는 영향(影響)

도진우 ( Jin Woo Do ),안성훈 ( Sung Hun An ),구성태 ( Sung Tae Koo ),백대봉 ( Tae Bong Baek ),김경식 ( Kyung Sik Kim ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.2

Objectives: Ulcerative colitis and Crohn`s diseases are chronic inflammatory disease of the gastrointestinal tract identified mainly for diarrhea, stomachache, hemafecia. The source and pathologic mechanism about ulcerative colitis and Crohn`s disease are still unknown but stress, infection, a genetic factor, environmental factors etc. may be the cause of chronic inflammatory disease of the gastrointestinal tract recently. Methods: Because ulcerative colitis and Crohn`s disease is recognized as Ha-ri(下痢) or Jang-Byok(腸벽) in Oriental Medicine, Rhizoma Coptidis Herbal acupunctures were injected in hapgok(LI4) which is used to treat intestinal disease in clinic. All animals were subjected to the injection of saline (300 ㎕, 500 ㎕) for a study control and TNBS (300 ㎕, 500 ㎕) into the lumen of the colon, 8cm proximal to the anus through the intestine. Rhizoma Coptidis Herbal (20 ㎎/㎖, 0.4 ㎖) acupuncture solution was injected to the LI4 (both hands) at the secondary injection time of TNBS in rats. And body weight, RBC count, WBC count, total protein, Paw edema rate, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels were observed to study the effects of Rhizoma Coptidis Herbal acupuncture in hapgok (LI4). Results: In results, Rhizoma Coptidis Herbal acupuncture in hapgok (LI4) on TNBS-induced colitis reduced the rate of weight loss but had no effect on RBC and WBC count. And it reduced total protein concentration, paw edema, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels. Conclusion: Data suggest that injection of Rhizoma Coptidis herbal acupuncture solution into hapgok has significant therapeutic effect on the colitis.

자침시(刺鍼時) 압침(押鍼)의 수기자극(手技刺戟)이 전위변화(電位變化)에 미치는 영향(影響)

송문영 ( Moon Young Song ),심원보 ( Won Bo Shim ),김영태 ( Young Tae Kim ),백대봉 ( Dae Bong Baek ),안성훈 ( Sung Hun Ahn ),김경식 ( Kyoung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.4

Objective: hypothesis. First of all, there exists electric property in meridian and meridian point. Second of all, energy flowing in meridian is related with electric property. Third of all, there is electronic interaction between the operator who performs acupuncture therapy and the receiver who is given acupuncture therapy. Forth of all, acupuncture effects may depend on the electric capacity which is transferred between the operator and the receiver via acupuncture needle. Methods: Under the hypothesis, I studied the effects of electric charge in ST37(+) and ST39(-) generated by Apchim (押鍼) manipulation which was stimulated at ST36 point. And compared with data on the changes of electric charge from the hand of the operator during acupuncture stimulation. Electric charge induced via acupuncture needle from the operator may be important factor that causes the changes of electric charge in meridian and acupoint in the receiver. Therefore we investigated the changes of electric charge induced by the operator using Maclab 400 by the following methods. The one was in stable electric circle condition and the other was in unstable electric circle condition. In this experiments, the changes of electric charge from the stimulation type of Apchim manipulation performed in our lab condition in acupuncture therapy was induced at least three factor, one was the difference of bio-potentials between the operator and the receiver of acupuncture therapy, another was the depth of acupuncture insertion from the skin of the receiver, the other was an electromyogram of the receiver. Results: The data imply that the first factor should make a capacitance current when the operator touched the acupuncture needle which was inserted in the receiver. Therefore, the results suggest that capacitance currents stimulus in electronic view may be important factor in acupuncture therapy between the operator and the receiver of acupuncture therapy.