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백수오(白首烏)와 비주화(啤酒花) 복합물의 에스트로겐 활성과 파골세포 분화 억제효과
박동준,이홍구,민경인,박형국,진무현,조호성,Park, Dongjun,Lee, Hong Gu,Min, Kyoungin,Park, Hyoungkook,Jin, Mu Hyun,Cho, Ho Song 대한본초학회 2022 大韓本草學會誌 Vol.37 No.5
Objectives : This study aimed to investigate the synergistic effect of combining Cynanchi Wilfordii Radix extract with Humuli Lupuli Flos extract on estrogenic and anti-osteoclastogenic activity. Methods : Estrogenic effect of a mixture of Cynanchi Wilfordii Radix extract and Humuli Lupuli Flos extract (CWHL), Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin (an active ingredient of Cynanchi wilfordii Radix extract) and 8-prenylnaringenin (an active ingredient of Humuli Lupuli Flos extract) were examined by proliferation E-screen assay and expression of estrogen inducible gene, pS2 via Real Time-PCR (RT-PCR) in MCF-7 estrogen responsive cells. And their estrogenic activities were investigated how to modulate Estrogen receptor 𝛽 by binding affinity assay. Inhibitory effect of CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin on RANKL-induced osteoclast differentiation were tested by TRAP (Tartrate-resistant acid phosphatase) staining in osteoclastogenic RAW 264.7 cells. Results : CWHL, Humuli Lupuli Flos extract and 8-prenylnaringenin accelerated the proliferation of MCF-7 and the expression of pS2 in MCF-7. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin bind to estrogen receptor 𝛽. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin inhibited RANKL-induced osteoclastogenesis in osteoclastogenic RAW 264.7. CWHL is more effective for all markers than Cynanchi Wilfordii Radix extract or Humuli Lupuli Flos extract alone. Conclusions : CWHL may a potential therapeutic agent for menopause and osteoporosis as a natural food resource. CWHL as a natural food source has therapeutic potential in cases of menopause and osteoporosis.
골담초꽃 추출물의 연골 보호 및 염증 억제 효과에 관한 연구
박동준,이홍구,고충호,박형국,진무현,조호성,Park, Dongjun,Lee, Hong Gu,Ko, Chung Ho,Park, Hyoungkook,Jin, Mu Hyun,Cho, Ho Song 대한본초학회 2022 大韓本草學會誌 Vol.37 No.6
Objectives : This research aimed to investigate chondro-protective and anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract and its compound, tilianin. Methods : Caraganae Sinicae Flos was extracted with 50% ethanol. Tilianin in Caraganae Sinicae Flos 50% ethanol extract was quantified by HPLC analysis method. To investigate chondro-protective effects of Caraganae Sinicae Flos 50% ethanol extract, ATDC5 chondrogenic cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and tumor necrosis factor-𝛼 (TNF𝛼) for 24 hours. After treatement for 24 hours, media supernatant was used for quantifying protein level of matrix metalloproteinase-3 (MMP3) by ELISA and harvested cells were used for analyzing mRNA expression level of matrix metalloproteinase-13 (MMP13) by reverse transcription PCR. To identify anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract, RAW 264.7 macrophage cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and lipopolysaccharide (LPS) for 24 hours. media was used for quantifying the level of prostaglandin E2 (PGE2), interleukin-6 (IL6) by ELISA and nitric oxide by Griess reagent asssay. Results : Caraganae Sinicae Flos 50% ethanol extract and tilianin attenuated protein level of MMP3 and mRNA expression level of MMP13 in TNF𝛼-activated ATDC5 cells. Caraganae Sinicae Flos 50% ethanol extract inhibited the level of PGE2, IL6 and NO in LPS-activated RAW 264.7 cells in dose dependent manner, though tilianin inhibited PGE2 only. Conclusions : These results presented that Caraganae Sinicae Flos 50% ethanol extract could be used as natural medicines for osteoarthritis.
마가자(馬家子) 추출물의 연골 보호 및 항염 효과에 관한 연구
박동준,이홍구,고충호,박형국,진무현,조호성,Park, Dongjun,Lee, Hong Gu,Ko, Chung Ho,Park, Hyoungkook,Jin, Mu Hyun,Cho, Ho Song 대한본초학회 2022 大韓本草學會誌 Vol.37 No.6
Objectives : From this study, we sight to identify chondro-protective and anti-inflammatory effects of Sorbi Commixtae Fructus extract and its compound, chlorogenic acid. Methods : Sorbi Commixtae Fructus were extracted by 50% ethanol. And chlorogenic acid in Sorbi Commixtae Fructus 50% extract was quantified by high performance liquid chromatography (HPLC). To investigate chondro-protective effects, we treated Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid in TNF𝛼-activated ATDC5 murine chondrogenic cells. After 24 hours, protein level of matrix metalloproteinase-3 (MMP3) and mRNA level of matrix metalloproteinase-13 (MMP13) were measured by using ELISA or reverse transcription PCR, respectively. To examine anti-inflammatory effects, we treated Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid in LPS-induced RAW 264.7 murine macrophages. We measured the level of inflammatory mediators, such as Prostaglandin E2 (PGE2), Interleukin-6 (IL6) by ELISA and nitric oxide (NO) by Griess reagent assay. Results : A concentration of chlorogenic acid in Sorbi Commixtae Fructus 50% ethanol extract was 3.9 mg/g. Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid attenuated protein level of MMP3 and mRNA level of MMP13 in TNF𝛼-activated ATDC5 cells. Sorbi commixtae Fructus 50% ethanol extract inhibited the level of PGE2, IL6 and NO in LPS-activated RAW 264.7 cells in dose dependent manner, but chlorogenic acid has no anti-inflammatory effects. Conclusions : These findings demonstrated that Sorbi Commixtae Fructus 50% ethanol extract has chondro-protective and anti-inflammatory effects showing possible therapeutics to ease the symptoms related with osteoarthritis.
실버세대 및 자율성 저하 노인을 위한 문화매개활동 연구 -프랑스어권 사례를 중심으로
박동준 ( Dongjun Park ) 연세대학교 유럽사회문화연구소 2016 유럽사회문화 Vol.0 No.16
Le guide se rattache au developpement d’approches de mediation culturelle destinees aux aines. Le guide de bonnes pratiques a ete realise dans le territoire de la Monteregie au Canada. Les personnes ainees : de qui parle-t-on? Il n’existe aucun consensus sur la definition d’une personne ainee. Dans une perspective de prevention et afin d’inciter a un vieillissement actif, on a besoin de certains moyens. L’art est en constante interaction avec la societe, la ou se tissent les liens entre politique, culture et espace public. Ce processus de mise en relation entre les spheres de la culture et du social est aussi connu sous le nom de mediation culturelle. On entend par activites de mediation culturelle des initiatives qui facilitent l’appropriation de la culture par les citoyens. Ces actions creent une opportunite de rencontres et d’echanges personnalises favorisant le contact des clienteles avec les oeuvres et les createurs. Elles permettent de faire le pont entre le citoyen et l’offre culturelle professionnelle. Les pratiques et les oeuvres artistiques dont il est question ici sont le fait de professionnels et ne doivent pas etre confondues avec celles relevant des loisirs culturels. Ainsi, les projets de mediation culturelle inities ou portes par des organismes ou des municipalites feront appel a des artistes ou a des travailleurs culturels professionnels. Dans la meme logique, il importe de comprendre la mediation culturelle comme un metier. a mesure que le champ de la mediation se concretise, les formations se developpent. Les mediateurs culturels developpent leurs competences en poursuivant des etudes universitaires ou en acquerant, comme artistes ou travailleurs culturels, une experience professionnelle.
CFD를 이용한 예배당에서의 COVID-19 집단감염 분석
박동준(Park, Dongjun),강경모(Kang, Kyungmo),김태연(Kim, Taeyeon) 한국생태환경건축학회 2021 한국생태환경건축학회 학술발표대회 논문집 Vol.21 No.2
Recently, there have been serious problems that were caused by the increasing cases of mass infection in public places. This research studies the specific outbreaks that occurred in a religious facility in Korea. CFD analysis uses the droplet nuclei diffusion method of the person who was infected the first. This study proves how indoor natural ventilation affects droplet concentration and airflow pattern. In conclusion, CFD analysis shows that the indoor airflow pattern affects the change in droplet concentration. In addition, natural ventilation reduces the droplet concentration by 21%.
대단면 석회석 광산 갱도 내 국부선풍기 승압력 및 통기효과 비교 연구
박동준(Dongjun Park),강현호(Hyeonho Kang),이창우(Changwoo Lee) 한국암반공학회 2017 터널과지하공간 Vol.27 No.1
국내 지하 석회석 광산은 대부분 대단면 갱도로 개발되고 있으며 갱내통기는 주요 선풍기에 의한 통기방식보다는 자연통기 및 국부통기에 의존하고 있다. 국부통기는 국부선풍기와 풍관을 이용한 급배기 통기방식을 적용하고 있으나 대단면 갱도 특성과 갱내 굴진․운반장비의 디젤화에 따라 소요통기량이 대폭 증대하여 통기의 효율 및 경제성 확보가 주요과제로 대두되고 있다. 본 연구에서는 대단면 갱내 작업장 국부통기를 위한 선풍기 용량 최적화를 위하여 축류식 및 프로펠러 선풍기를 개발하여 갱내 승압력 및 통기효과를 비교 분석함을 목적으로 한다. Most of the local limestone mines are developed as large-opening underground mines, while mine ventilation is heavily dependent on the natural ventilation and auxiliary systems, rather than the mechanical ventilation system using main fans. The current auxiliary ventilation system with fan and ducting requires optimization since enhanced deployment of diesel equipment demands higher airflow rate and the associated cost is expected to be too excessive for the local mine operators. This paper aims at optimizing the fan capacity for the working site ventilation through comparing the fan pressure in the mine airway and the ventilation efficiency of an axial-flow fan and a propeller fan developed in this study.