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동충하초 재배상 내 기형균 및 오염균의 발생현황 및 특성
남성희 ( Nam Seong Hee ),윤철식 ( Yoon Cheol Sik ),정이연 ( Jung I Yeon ),지상덕 ( Ji Sang Duk ),조세연 ( Cho Sae Yun ),한명세 ( Han Myung Sae ) 한국잠사학회 2002 한국잠사곤충학회지 Vol.44 No.1
7개도 22개 재배농가로부터 수집한 시료는 총 529점이었으며, 수집균은 두 가지 유헝으로 구분되며 생육장애에 의한 기형균은 23.2%, 오염균은 50.9%였다. 생육장애는 C-1, C-2, C-3 및 C-4의 4가지 유형이 있으며 이들 분생자병속은 20∼70여 개가 발생되는데 회색, 순백색 또는 등황색을 띤다. 분생자병속 말단은 분지하지 않고 기주 표피에 각각 고유색상을 띠며 PDA 배지상에서 균사생장량은 14일간 43∼75 mm 범위로 눈꽃동충하초균보다 빠른 생장을 보인다. Occurrence and charaberistics of infectious fungi and physiologically abnormal Paecilomyces tenuipes were examined. These unfavorable fungi are recently known to cause yield loss, decreased quality of products in artificial cultivation farms of P. tenuipes. Total 529 fungal isolates were collected from 22 farms of 7 provinces. These isolates were composed of physilolgically abnormal P. genuipes strains and other infectious fungal strains, which constituted 23.2% abd 50.9%,k resoectuvekt. Physiologecally abnormal P. tenuipes strains showed irregular synemata,k and absence of local color. They were divided into 4 type of C-1, C-2, C-3,and C-4. Other infectious fungal strains were idevified to 5 species,Bearveria bassiana, Fusarium sp., P.fumosoroseus, Tricothecium roseum,k Aspergillus parasiticus,k which were 22.1, 13.8, 7.6, 40. and 3.4%, respectively. All of them were hyphomycetous fungi, did not produce synemata, and revealed faster growth rate than that of P. tenuipes.
남성희 ( Nam Seong Hui ),정이연 ( Jung I Yeon ),지상덕 ( Ji Sang Duk ),조세연 ( Jo Se Yeon ) 한국잠사학회 2001 한국잠사곤충학회지 Vol.43 No.2
현재까지 곤충기생균 고유배지가 개발되어 있지 않아 식물기생균 배지를 대체 이용해 옴에 따라, 본 시험에서는 고단백질원으로써 이용가치가 있는 누에분말을 이용하여 곤충기생균 최적배지를 개발코자 하였다. 그 결과 사면배지상에서 균 배양시 12종의 배양형태는 현상, 융기, 주변형태가 균종 및 배지에 따라 배양 상이 동일 혹은 다양하게 형성되었다. 균주별 생장율은 12 균주 중 C. militaris를 제외한 11 균주가 SLA배지에서 PDA에 비해 대부분의 균생장이 양호하였으나 C. militaris는 기존배지와 비교해 생장률이 78.6%로 감소하였다. Dextrose 첨가시 최적 농도는 C. gracilioides 등 6균주에서 6% 처리시 가장 높은 생장률을 나타내엇다. 본 시험결과 선발된 배지로부터 배양균을 누에접종 후 재배 시험한 결과 감염율, 자실체 형셩율, 분생자경수 및 분생자경의 길이는 91.3±1.5%, 88.6±2.3%, 56.6±3.2개, 33.4±2.8mm로 대조에 비해 모두 높게 나타났다. 본 시험에서 개발된 Dextrose를 첨가한 SLA-A배지의 경제성 분석결과 1ℓ 생산시 기존배지가 6,261원이 소요되는 것에 비해 총 2,386원으로 생산이 가능해 본 배지로 대체시 61.9%의 절감효과를 나타내었다. This study was carried out to investigate the optimal medium and optimal dextrose concentration of 12 entomopathogenic fungi. The colonies of cordyceps gracilioides, C. sp,(J23), Paedilomyces farinosus(J5), P. tenuipes, Paecilomyces sp.(J4), Beauveria bassiana and B. brongniartii on SLA(silkwon larva agar)medium are similar to those on PDA(potato dextrose agar) medium. On the contrary, the colonies of fungi are very various according to its medium. The optimal medium for 11 fungi was SLA-A medium, which was better than PDA medium with regard to the mycelial growth. The optimal concentration on mycelial growth was superior to 6% for 6 fungi. When silkworm larvae was injected with P. tenuipes on SLA medium for fruiting body production. Infection rate, fruiting body formation rate, synnemata number and synnemata length were 91.3±1.5%, 88.6±2.3%, 56.6±3.2and 33.4±2.8mm, respectively. In economical analysis, the prototype medium can reduce 61.9% of production cost per 1 liter compared with PDA medium.
남성희 ( Sung Hee Nam ),이광길 ( Kwang Gill Lee ),여주홍 ( Joo Hong Yeo ),이희삼 ( Heui Sam Lee ),황재삼 ( Jae Sam Hwang ),최영철 ( Young Cheol Choi ),박관호 ( Kwan Ho Park ) 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.2
Cordyceps and its allies fungi has been described as a secret medicine that gives eternal youth and a long life. Some species of Cordyceps are sources of biochemicals, such ascordycepin, with interesting biological and pharmacological properties. Hence, it has been studied to uncover its pharmacological effect. We attempted to study the formation of fruiting bodies and to develop means of mass production Korean isolate of Paecilomyces tenuipes has been inoculated into silkworms, where it reproduced using culture container, mini-kit successfully. Culture container, mini-kit is composed of a cylinder-shaped body and lid. The container is made of translucent polyethylene terephthalate. The size of the container is 82 × 75 mm, reduced by 10 times as compared with the conventional culture kit. The mini kit has many advantages - high culture amount, ability of maintaining optimal humidity, parasite-free cultivation and high-end appearance. With the kit, the optimal cultivation condition is under 22oC, culture period of 53 days. And synnemata of P. tenuipes could be kept fresh for 14 days at the temperature of under 10oC. Therefore, the Min-kit can be used in both ways as a culture container and a packing kit for end-user customers.
백묵병 감염유충으로부터 병원균의 분리 및 형태학적 특성
남성희(Sung Hee Nam),이명렬(Myeong Lyeol Lee),이만영(Man Young Lee),김영수(Young Soo Kim),이병일(Ri Bing Li),장승종(Seung Jong Chang),윤병수(Byoung Su Yoon) 한국양봉학회 2004 韓國養蜂學會誌 Vol.19 No.1
Chalkbrood-infected larvae of honeybee were obtained from 8 apiaries in Korea. Total 548 samples varied in the rate of black and white mummies of honey bee. Among them, 306 black mummies were examined to identify species. The whole larvae were proved to be infected with Ascosphaera apis(Maasen ex Claussen) Olive & Spiltoir. In the early stage of chalkbrood diseases, the white/grey mass was formed on the surface of larvae. The larvae were soon shrunken to mummy, getting hard, and forming hard mummies. In the late stage of chalkbrood diseases, the mycelium grew densely and covered the larvae to the extent that it filled the whole cell. When the larvae were infected with one sexual type of A. apis, they became white mummies while the grey-black mummies were developea by the invasion of both sexual types. A. apis (Aaj623) was heterothallic and mostly grown 57.6 ㎜ in diameter in 7 days on potato dextrose agar(PDA). Spore cysts were globose, nearly smooth on outer surface and evenly verrucate on inner surface, 45~95 ㎛ in diameter(average 74 ㎛ diameter). Spore balls were globose, 9~17 ㎛ in diameter(average 12.9 ㎛ in diameter) and lacking a conspicuous granular coating. Ascospores were hyaline, ellipsoid and smooth, 1 cell, 2.3~3.0 × 1.0~1.5 ㎛(average 2.49 × 1.35 ㎛ in diameter).
꿀벌(Apis mellifera) 유충 및 사육환경으로부터 백묵병원균(Ascosphaera apis)의 진단
남성희(Sung Hee Nam),이명렬(Myeong Lyeol Lee),최지영(Ji Young Choi),황재삼(Jae Sam Hwang),이만영(Man Young Lee),김영수(Young Soo Kim),이혜민(Hye Min Lee),윤병수(Byoung Su Yoon) 한국양봉학회 2005 韓國養蜂學會誌 Vol.20 No.1
Chalkbrood of honeybee, Apis melifera is the highly-infectious disease which is caused by Ascosphaera apis, entomopathogenic fungi occurring only honeybee larval stage. The environment of disease occurrence has high humid and temperature in the hives. Dead brood is founded on the ground around hives as well as in the combs. A rapid and sensitive detection of Ascosphaera apis in honeybee larva, hive and their breeding environment were carried by polymerase chain reaction(PCR). The combination of primers of CBP1 and CBP3 was superior in PCR to the other 3 primers applied. Each primer about 20 base in size has reported as specific primers. PCR products appeared 500bp in size. In case of the detection of A. apis from the infected larva, soil, debris and pollen, sensitivity by PCR were higher than that of microscopic observation.