한국재래산양에서의 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 II. 혈청내 Testosterone수준의 계절 및 하루중 변화

김창근,정영채,김광식,김수,권처진,한기영,윤종택,정영호,이장희,Kim, C.K.,Chung, Y.C.,Kim, K.S.,Kim, S.,Kwon, C.J.,Han, K.Y.,Yoon, J.T.,Chung, Y.H.,Lee, J.H. 한국동물번식학회 1994 Reproductive & developmental biology Vol.17 No.4

Seasonal and diurnal variation in serum testosterone was measured by radioimmunossary in 3 mature Korean native male goats. Blood was collected at 3-h intervals for 24h in each season. Mean levels of 4 seasons ranged from 0.82 to 2.06ng/ml and no difference was among seasons, although level in fall and winter was slightly higher. Diurnal level tended to increase at daytime in all season and when diurnal variation in level was compared to daily mean of each season, level of daytime greatly(P<0.05) increased in summer. However, variation between light and dark period in other seasons did not show significant difference. Daytime increase of testosterone was found 3 to 4 after dawn and level increased 1 to 2h ahead in summer and fall than that in others. These results in serum testosterone suggest that there was no great seasonal variation in endocrine function of goat testis.

한국재래산양에서 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 I. 정액성상과 정자의 내동성 및 난자침입능력의 계절적 변화

김창근,정영채,김광식,윤종택,이장희,정영호,최선호,김흥률,김수,권처진,Kim, C.K.,Chung, Y.C.,Kim, K.S.,Yoon, J.T.,Lee, J.H.,Chung, Y.H.,Choi, S.H.,Kim, H.Y.,Kim, S.,Kwon, C.J. 한국동물번식학회 1994 Reproductive & developmental biology Vol.17 No.4

This study was conducted to observe seasonal and individual changes in semen characteristics and sperm freezability, and sperm penetration into zona-free hamster eggs in Korean native goats. Buck response and change in semen characteristics to electrical stimulations was evaluated for four seasons throughout 2 years and percentage of motile sperm and normal apical ridge acrosome was investigated after equilibration and thawing for 4 seasons with 5 bucks. Sperm penetration rate was evaluated for 4 bucks. 1. Probe insertion at depth of 7cm and repeated stimulation for 3 sec was more effective(P<0.05) in buck response and semen collection than those of other conditions. 2. Semen characteristics from electrojaculation was signficantly(P<0.005) higher in spring and fall for semen volume, in spring and summer for sperm concentration and in fall for sperm motility than those in other seasons, respectively. However, there were no differences in total sperm among seasons. 3. Buck response to electrical stimulation showed significant difference(P<0.05) among individuals in all 3 seasons except winter. Significant individual difference in semen volume was only in spring and summer, but there was no indivudual difference in sperm concentration and total sperm in all season. 4. Washing of semen before freezing treatment was greatly(P<0.05) beneficial to sperm motility after thawing, no matter whether ejaculates exhibit egg yolk coagulation or not. 5. Sperm motility after glycerol equilibration was significantly(P<0.05) low in summer semen and motility after thawing was greatly(P<0.05) higher in winter semen than in other seasons. Freezability of unwashed sperm was significantly difference among bucks, but a yearly freezability of washed sperm after chilling and thawing were no differences among bucks and percentage of normal apical ridge acrosome were not different among seasons and bucks. 6. There was no significant difference in sperm motility after thawing between egg yolk levels in summer, although 20% level gave more higher motility than 5% level. 7. In summer, 3.2% glycerol and 3-h equilibration gave greatest percentage(P<0.05) of sperm motility and normal apical ridge acrosome after thawing. 8. Sperm penetration rate into zona-free hamster eggs was not different between bucks and seasons. Overall, it is concluded that to obtain maximum sperm output and successive semen freezing by electrojaculation method, buck selection with good response in all season could be basically considered and that seasonal effect on sperm freezability was more greater than that of individual bucks.

영상처리를 이용한 복합재료 내의 단섬유 배향상태의 측정

김창근,고진,윤재륜 ( Chang Keun Kim,Jin Ko,Jae Ryoun Youn ) 한국복합재료학회 1993 Composites research Vol.6 No.1

산란중 Testosterone Propionate 처리가 계배자와 병아리의 발육 , 생식기 및 갑상선발달에 미치는 영향

김창근,이용빈 ( Chang Keun Kim,Yong Bin Lee ) 한국축산학회 1978 한국축산학회지 Vol.20 No.1

These experiments were conducted to study the effects of testosterone propionate (TP) treatment during incubation on the embryonic (Experiment 1) and postnatal (Experiment 2) development of body, genital organs and thyroid gland in chicken. In the experiment L, RIR embryos treated with TP at the 2nd or 10th day of incubation were observed at 10, 14 and 18-day stage. In the experiment 2, RIR(♂) × Columbian Plymouth Rock(♀) chicks hatched from TP-treated eggs at the 10th day of incubation were observed at monthly intervals from 1 to 5 months of age. The results obtained in the experiment 1 and 2 are summarized as follows: Experiment 1. 1. The weight of embryo after TP treatment was found to be distinctly different between the control and TP-treated group at the 2nd day treatment than at the 10th day treatment of incubation ana the reduction of embryo weight was greater at the higher levels of TP. Especially, this reduction of male embryo weight was somewhat larger than that of female throughout all stages of incubation and the weight of 18-day embryo was significantly smaller in all TP-treated groups than in the control group. The length of using and leg of male and female embryo was considerably shorter at the latter half period of incubation in the TP-treated groups and the development of these parts were more retarded at the 2nd day treatment of TP. 2. The embryonic mortality from the day of TP treatment to 18-day stage was exceptionally higher in the 2nd day treatment than in the 10th day treatment, the percentage of which was 52.3∼60.5% for the 2nd day treatment with 1∼2㎎ TP and 30.7∼40.2% the 10th day treatment with 3∼4㎎ TP. More than 60% of dead embryos appeared to be dead within a few days after TP treatment. 3. The reduction of testis size of embryo related to TP treatment was more remarkable at 14-day stage than at 18-day stage and in the testis size at 18-day stage, no significant difference was observed between the control and TP-treated group, except for the higher levels of TP. When the embryos were treated with the high levels of TP the volumetric proportion of seminiferous tubules in 14 and 18-day embryo testes was. smaller than those of control group, while the diameter of tubules in the TP-treated groups was only smaller at 18-day embryo. 4. The size of left ovary in the TP-treated embryo was smaller in the 2nd day treatment than in the 10th day treatment. The ovary size at 14 and 18-day stage was smaller in all TP-treated groups than in the control group and the ovary size of 18-day embryo treated at the 2nd day of incubation was not especially increased compared with that of 14-day embryo. In the 2nd day treatment the cortex thickness cf left ovary of 14 and 18-day embryo was thinner in the higher levels than in the lower levels and was inactive in the division of germ cells and in the development of secondary sex cords in the cortex. 5. The length of left oviduct in the 14 and 18-day embryo was shorter only in the groups treated with higher levels of TP. However, the reduction of length of right oviduct was similar to those of control throughout the stages of embryo, although the length of oviduct was more reduced in 2㎎ TP-treated group at the 2nd day treatment. The regression of right oviduct was not affected by TP treatment. 6. The thyroid weight of 18-day embryo was not different between sexes and the reduction of thyroid w eight was greater at high levels of TP treatment. The follicle size of embryo thyroid was smaller in the TP-treated groups than in the control group at the 2nd and 10th day treatment and the epithelial cell height of embryo thyroid was not found to be distinctly affected by the time or level of TP treatment. The size of bursa of Fabricius in 18-day embryo was significantly smaller in all TP-treated groups than in the control group. The regression of bursa was larger in the 2nd day treatment and in male embryo and showed difference among TP treated groups in both sexes.

Radiofluorinated Ethyluracil과 Deoxyadenosine을 이용한 종양세포 증식의 영상화에 대한 연구

김창근,양경문,김의신 ( Chang Guhn Kim,David J . Yang,E . Edmund Kim ) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4

미경산돈의 발정유기 및 혈중 Progesterone 수준변화에 관한 연구

김창근,정영채,정영호,김형태 ( C . K . Kim,Y . C . Chung,Y . H . Chung,H . T . Kim ) 한국축산학회 1982 한국축산학회지 Vol.24 No.3

These experiments were conducted to investigate the effects of PGF₂α injection on induction of estrus and change in blood progesterone concentration in normal and anestrous gilts. Six anestrous gilts and 5 normal gilts were used. For induction of estrus either 10㎎ PGF₂α or 10㎎ PGF₂α plus 1,000IU PMSG was intramuscularly injected. Serum progesterone concentration were determined by radioimmunoassay. Conception of gilts were confirmed by necropsy examination for ovary and uterus condition within 35 days after mating. The number of gilts which showed standing or dull estrus after either PGF₂α or PGF₂α plus PMSG injection was 5(83.3%) out of 6 long-anestrous gills and 4(80.0%) out of 5 normal gills. The average interval from PGF₂α injection to the induced estrus was 7.2 days (4 to 12 days) in 5 long-anestrous gills and 13.5 days (6 to 25 days) in 4 normal gills. The number of pregnant gills was 2 out of 4 long-anestrous gills and 3 out of 4 normal gills, except each one get in two groups which was necropsied too early to be confirmed whether pregnant or not, although their ovary and uterus were normal condition. Blood progesterone concentration of 11.0 ng/㎖ before PGF₂α injection in 5 longanestrous gills which showed estrus decreased to 3.0 ng/㎖ at 1 day and 1.3 ng/㎖ (11.8% of initial concentration) at 4 days and increased again to 4.3 ng/㎖ at 8 days after PGF₂α injection, respectively and the concentration of 16.5 ng/㎖ before PGF₂α injection in 4 normal gills which showed estrus decreased to 3.2 ng/㎖ at 1 day and 2.3 ng/㎖ (13.9% of initial concentration) at 4 days and increased to 4.7 ng/㎖ after PGF₂α injection, respectively. Blood progesterone concentrations at 10 to 15 days and 30 to 35 days after mating in pregnant gills were 8.2 to 26.4 ng/㎖ and 17.7 to 19.6 ng/㎖ in 2 anestrous gills and 23.1 to 28.5 and 19.6 to 19.9 ng/㎖ in 3 normal gills respectively. The number of corpus luteum in the induced estrous gifts averaged 18.8 in 3 longanestrous gifts and 15.0 in 4 normal gifts. There would appear to be larger number of CL in PGF₂α plus PMSG injection than in only PGF₂α injection.

과학영재교육기관(科學英才敎育機關)을 위(爲)한 도서관기준설정(圖書館基準設定)에 관(關)한 연구(硏究)(I)

김창근,Kim, Chang-Keun 한국정보관리학회 1987 정보관리학회지 Vol.4 No.1

국가발전의 원동력으로서의 과학기술밭전을 위한 국가적 조치로서 영재교육기관을 설립하고, 교육을 통한 과학영재의 발굴, 육성을 시도하고 있는 싯점에서 교육기관의 환경적 요인이 크게 중요시되고 있다. 그 환경요인 중에서도 지식의 보고로서의 도서관 환경조성이야 말로 소기의 목표를 달성하고 그 시기를 더욱 앞당길 수 있는 가장 중요한 요인이라 할 수 있기 때문에, 본 논문에서는 과학영재교육의 중요성을 기술하고 그 교육을 위한 도서관의 환경적 개선요인을 조사, 비교 분석하여 과학영재교육 기관을 위한 도서관 기준을 시설, 인원, 장서, 예산등의 포괄적인 기준에 관한 합리적 타당성을 도출하고 최적 기준을 제시하였다. Many countries make it their national policy to develop science and technology as the primary element in their overall national development, they concentrate on identifying gifted students in those areas and on educating them in special institutions. At this point the environmental factors in those educational institutions are highlighted. Of all these factors, a favorable and amenable environment of a library, the treasure - house of knowledge, is the most essential to the early achievement of the stated educational aims. Thus, this thesis describes the importance of educating gifted students, and then examines, compares and analyzes the factors involved in improving the environments of institutional libraries for gifted students ; finally, this thesis proposes the minimum and optimum standards for library facilities, personnel, collections, and budgets.

초대형 원문정보데이타베이스 ADONIS

김창근,Kim, Chang-Geun 한국도서관협회 1993 圖協會報 Vol.34 No.2

2003년판 한국도서관기준의 의의와 특징 - (3)전문도서관

김창근,Kim, Chang-Geun 한국도서관협회 2004 圖協會報 Vol.45 No.2

CD-ROM화된 중화민국 국립중앙도서관의 중국서지시스템

김창근,Kim, Chang-Geun 한국도서관협회 1991 圖協會報 Vol.32 No.6

본 고는 1969년 4월 창간된 중화민국 대북시 소재 국립중앙도서관 뉴스레터인 국립중앙도서관통신 Vol. 22, No, 4(1991, 2)에 수록된 기사를 번역한 것이다. 역자는 경제수준으로 비교할 때 항상 우리나라와 경쟁관계에 있는 대만이 정보분야 정책입안과 실행에 있어서는 우리의 현실보다 훨씬 더 앞서 있다는 사실에 부러운 한편 아연해하는 심정으로 본 기사내용을 도서관계 여러분에게 보다 널리 알리고 싶어서 도서관문화의 지면을 빌어 소개하고자 합니다.