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청갓과 적갓에 함유된 Glucosinolates의 항암 활성 및 정량 분석
김활 ( Hwal Kim ),김준열 ( Jun Yeol Kim ),김효진 ( Hyo Jin Kim ),김도경 ( Do Kyung Kim ),조혜진 ( Hye Jin Jo ),한병수 ( Byoung Su Han ),김헌웅 ( Heon Woong Kim ),김정봉 ( Jung Bong Kim ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.3
The objective of this study was to compare the anticancer activity of glucosinolates against four different cancer cells; SNU-251, SNU-354, SNU-C4, MCF-7 and to determine the amounts of glucosinolates in mustard leaves. Green and red mustard leaves were cultivated on the field of Rural Development Administration from May to July, 2010. After the leaves were harvest and lypophilized, the fine powder was extracted with boiling 70% methanol(v/v) for the measurement of anticancer activity and then applied to the cancer cells obtained from Korean Cell Line Bank(KCLB). The anticancer activity of red leaf mustard was higher than that of green mustard leaf, particularly in SNU-251 for 24 hr and in SNU-C4 for 48 hr. In both green and red mustard leaf, sinigrin was the most abundant glucosinolte and was determined as 4.71 and 3.06 ㎎/g dry wt., respectively, but glucoiberin only presented 0.14 ㎎/g dry wt. with minor amounts.