관절염 모델에서 가미청열사습탕(加味淸熱瀉濕湯)의 진통 및 소염 효과에 관한 연구

김일현 ( Il Hyun Kim ),이하일 ( Ha Il Lee ),이세원 ( Se Won Lee ),권영미 ( Young Mi Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2015 한방재활의학과학회지 Vol.25 No.1

Objectives This study was carried out to find the effects of Gami-cheongyulsaseub-tang (hereinafter referred to GCST) on the inhibition of zymosan-induced pain in rats and collagen II-induced arthritis (CIA) in DBA/1J mouse. Methods As an acute inflammatory pain model, peripheral inflammation was induced by intraplantar injection of zymosan into the right hind paw in rats and then the hyperalgesia and pain regulating factors in spinal cord were analyzed. As a chronic inflammation model, the mixture of collagen II and complete Freund``s adjuvant was treated into mice to establish rheumatoid arthritis and then body weight, thickness of hind paw, pathological change of spleen, immunological rheumatoid factor (IgG1, IgG2a, IgG2b, IgM and anti-collagen II), pro-inflammatory cytokines, and bone injury were analyzed. Results In the acute inflammatory pain model, GCST significantly inhibited the thermal and mechanical hyperalgesia and the pain regulating factors, including Fos, CD11b, PKA and PKC, in the spinal cord with a dose-dependent manner. In the chronic rheumatoid arthritis model, GCST administration decreased arthritic index and paw edema as compared with CIA control group. In particular, GCST reduced significantly the serum levels of total IgG2a, IgG2b, IgM, and specific anti-collagen II, but not total IgG1. GCST also resulted in the attenuation of bone injury and spleen enlargement/adhesion in CIA mice. Moreover, the secretion of pro-inflammatory cytokines TNF-α and IL-1β in CIA mice was significantly reduced by GCST in a dose-dependent manner. Conclusions Comparison of the results in this study showed that GCST had anti-nociceptive and immunomodulatory effects. These data imply that GCST can be used as an effective drug for not only rheumatoid arthritic pain but also other auto-immune diseases. (J Korean Med Rehab 2015;25(1):27-44)

(무기환)戊己丸의 RAW 264.7 세포에 대한 항염증작용 연구

김일현 ( Il Hyun Kim ),최종환 ( Chong Hwan Choi ),이세원 ( Se Won Lee ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2013 한방재활의학과학회지 Vol.23 No.3

ObjectivesThe aim of this study was to investigate anti-inflammatory activity of Mugi-hwan (MGH) Water Extract. MethodsCells were treated with 2 ug/ml of LPS 1 hour prior to the addition of MGH. Cell viability was measured by MTS assay. The production of NO was determined by reacting cultured medium with Griess reagent. The expression of COX-2, iNOS and MAPKs was investigated by Western blot, RT-PCR. The content of level of cytokines (PGE2, IL-6, in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. ResultsMGH inhibited the production of NO, PGE2, IL-6 as well as the expressions of iNOS, COX-2 in the murine macrophage, RAW 264.7 cells. MGH also had suppression effects of LPS induced MAPKs activation. ConclusionsThese results suggest that MGH has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes. (J Korean Med Rehab 2013;23(3):27-35) ObjectivesThe aim of this study was to investigate anti-inflammatory activity of Mugi-hwan (MGH) Water Extract. MethodsCells were treated with 2 ug/ml of LPS 1 hour prior to the addition of MGH. Cell viability was measured by MTS assay. The production of NO was determined by reacting cultured medium with Griess reagent. The expression of COX-2, iNOS and MAPKs was investigated by Western blot, RT-PCR. The content of level of cytokines (PGE2, IL-6, in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. ResultsMGH inhibited the production of NO, PGE2, IL-6 as well as the expressions of iNOS, COX-2 in the murine macrophage, RAW 264.7 cells. MGH also had suppression effects of LPS induced MAPKs activation. ConclusionsThese results suggest that MGH has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes. (J Korean Med Rehab 2013;23(3):27-35)

소독음(消毒飮)에탄올 추출물이 사람각질형성세포 HaCaT 세포주의 자외선 손상에 미치는 영향

전규상 ( Kyu Sang Jun ),김일현 ( Il Hyun Kim ),임광묵 ( Gwang Mook Lim ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2012 한방재활의학과학회지 Vol.22 No.3

Objectives :Sodok-eum(xiaodu-yˇln, SDU) exhibits potent anti-inflammatory activity with an unknown mechanism. The purpose of this study is to elucidate the molecular mechanisms of SDU on pharmacological and biochemical actions in inflammation. Methods :We examined the effect of SDU on pro-inflammatory mediators in lipopolysaccharide(UVB)-stimulated HaCaT. The investigation focused on whether SDU inhibited cyclooxygenase-2(COX-2), interleukin-6(IL-6), interleukin-8(IL-8) and mitogen-activated protein kinases(MAPKs) in UVB-stimulated HaCaT cells. Results :SDU inhibited UVB-induced IL-6, IL-8 productions as well as the expressions of COX-2. Furthermore, SDU suppressed the UVB-induced phosphorylation of extracellular signal-regulated kinase 1/2(ERK1/2), c-JUN N-terminal kinase 1/2(JNK 1/2), p38. Conclusions :These results suggest that SDU has inhibitory effects on UVB-induced IL-6, IL-8 production, as well as the expressions of COX-2 in the HaCaT. The inhibitory effects occur through blockades on the phosphorylation of MAPKs following activation.

삼황사심탕(三黃瀉心湯)이 난황 알부민으로 유도된 알레르기 Mouse모델에서 항알레르기 효과

최종환 ( Chong Hwan Choi ),금선오 ( Seo Oh Keum ),이세원 ( Se Won Lee ),김일현 ( Il Hyun Kim ),이하일 ( Ha Il Lee ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.3

Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-α and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-γ, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients. (J Korean Med Rehab 2014;24(3): 71-85)

18세기 이탈리아의 지도제작의 전통과 조반니 마리노니의 역사적 위상에 관한 연구

김일현,김관수,Kim, Il-Hyun,Kim, Kwan-Soo 한국건축역사학회 2012 건축역사연구 Vol.21 No.5

This research focuses on the role of Giovanni Marinioni during the formation of the modern cartography and cadastre during the 18th century. Initial study began with Giambattista Nolli's Roman map noticing not much information was available to acknowledge his activities during his Milan period before the departure to Rome. It became evident that Marinoni was a key person to understand the complex circumstances in which the professional training and formation of Giambattista Nolli took place as later worked as an anonymous intern during the elaboration of Theresian Cadastre of Milan. The other important figures are Leandro Anguissola and Giovanni Filippini. Anguissola's position and precedent work facilitated Marinoni's multidisciplinary activities that he had performed in Vienna and Milano in the field of making urban maps of those two cities. On the other hand, Filippini not only collaborated with Marinoni but also introduced Nolli in the field of cartography. These activities show transitional and dual aspects that characterized the period in which important irreversible changes that occur during the reign of Habsburg empire and in the rest of the Europe toward the formation of modern society and state. Marinoni's theories and praxis greatly influenced Nolli's later commitment under the Savoia and later on the elaboration of the 'Pianta Grande di Roma' in 1748.

카를로 몰리노의 공간구성 방식에 관한 연구 - 주요 작품의 개념적 전제와 계획적 전략을 중심으로 -

김일현,Kim, Il-Hyun 한국주거학회 2007 한국주거학회 논문집 Vol.18 No.6

This study aims to analyse the method and strategy of spatial composition in the selective works of Italian architect Carlo Mollino. With the continuos renovation of the interpretative frames and method on modernity and in particular on the spatial phenomenon, works of Mollino, once disregarded by the historiography and evaluated for its peculiarity. emerge as important architectural phenomenons. The intrinsic value in his works also enable to comprehend the complexity and multiplicity of modernity in contemporary architecture. In order to achieve this preposition, six important works were analyzed with three key words, to understand comprehensively the process and the significance of each work and Mollino's contribution in the spatial composition of domestic and public spaces. Evaluation of the spatial strategies in conceptual investigations of Mollino had been underestimated to the relative poor conservation or incomprehension of various work, excluded from the categories of architectural elaborations. This study also aims to contribute, in the broad sense, the dialectical significance of modernity by emphasizing the aspects of his work related with Surrealism and vernacular tradition, as well as with its relationship with the various spatial dispositives.

제2차 세계대전 이후 이탈리아 주거정책과 건축문화의 역사적 고찰 - 이탈리아 국립보험공단 주택국의 주거정책을 중심으로 1949-1963 -

김일현,Kim, Il-Hyun 한국주거학회 2008 한국주거학회 논문집 Vol.19 No.6

Public housing problem became a major issue in each European country during the period of the reconstruction. This study focuses on the one of the major institutional policies in the public housing sectors in Europe during the second postwar period, specifically the INA (Istituto Nazionale delle Assicurazioni)-Casa in Italy from 1949 to 1963. Thanks to the financial Aid provided by 'Marshall Plan' of United States government, Italy initiated INA-Casa housing program elaborated and realized by minister of Labour, Amintore Fanfani. He was strongly influenced by catholic socialism and Weberian corporatism, and also had previously written theoretical studies on the subject during the Interwar years. The outcome of this policy had been devaluated for its Keynesian aspect as well as vernacular predominance in the architectural forms. On the contrary, this study focuses on themes such as continuity of politics before and after the Liberation, status of the architects and their will to social engagement, notion of quarter as 'self-sufficient community' and 'residential unit'. These factors will allow to comprehend the complex nature of the reality where different categories were involved to resolve the common housing problem.

선전과 소통 : 루치아노 발데사리의 브레다 전시관 계획에 관한 고찰 1951-1963

김일현,Kim, Il-Hyun 한국건축역사학회 2008 건축역사연구 Vol.17 No.6

Main theme of this paper is the evaluation of the historical significance of Luciano Baldessari's exhibition works mainly during the Fifties. In order to understand the formal and logical basis of those project, his relationship with the historical Avant-gardes and the consequences expressed in his pavilion projects for the Breda Industrial Company are analyzed. The first part focuses on the influence of Italian Futurism, German Expressionism and Italian Rationalism on the formation and experience of Baldessari during the interwar years. The encounter with these movements determine the interest but also the principles along which Baldessari represents his idea of object, space and place. Specially his professional activities during Fascism would determine his attitude toward political power and the necessity of autonomy in artistic sphere. In the second part, different themes that Baldessari affronted in each project of Breda Pavilions is analyzed. Another important issue regard the historiography of the contemporary architecture. Often, many important works that lies in the threshold of the disciplines such as painting and sculpture and media art were excluded in the history of architecture just because they do not deal with the architectural object. Recently, many elements such as theatrical project and temporary objects are considered as part of urban reality and architecture with acknowledgment of their capacity to create event and situations. Along this thought, not just the reconfiguration on the territory of architecture in present but also the criterion to evaluate the past history of architecture is changing drastically. This study on the pavilions of Baldessari intends to contribute indirectly on the current issue of dominion of architecture, but also to evaluate objectively recent architecture. Consequently, architectural protagonists such as Baldessari and their ephemeral projects would be evaluated compressively in their multiplicity of significance.

국지기후가 잣나무 성숙임분의 생식생장에 미치는 영향분석

김일현(Il Hyun Kim),신만용(Man Yong Shin),김영채(Young Chai Kim),전상근(Sang Keun Chon) 한국농림기상학회 2001 한국농림기상학회지 Vol.3 No.4

This study was conducted to reveal the effects of local climatic conditions on reproductive growth in a mature stand of Korean white pine based on climatic estimates. For this, the reproductive growth such as production and characteristics of cone and seed were first measured and summarized for seven years from 1974 to 1980. The local climatic conditions in the study site were also estimated by both a topoclimatological method and a spatial statistical technique. The local climatic conditions were then correlated with and regressed on the growth factors to reveal the relationships between the climatic estimates and the reproductive growth. Average number of conelet formation per tree showed highly negative correlation with some climatic variables related to minimum temperature in the year of flower bud differentiation. Especially, the most significant negative correlation were found between average of the minimum temperature for June and July of flower bud differentiation year and the number of conelet formation. There was no significant correlation between the number of cone production and climatic variables. However, total precipitation from December of the flowering year to February of the cone production year showed the most high correlation (r=0.6036) with the number of cone production. It was found that significant climatic variables affecting the amount of cone drop and cone drop percentage were the sum of cloudy days from June of the flowering year to August of the cone production year. Positive correlation was significantly recognized between the average weight of empty seed per cone and total precipitation from December of the flowering year to February of the cone production year. For the percentage of empty seed, five climatic variables among 19 variables were significantly correlated at 10% level. The average weight of a cone showed negative correlation with total precipitation from June of the flowering year to August of the cone production year. It was also found that average weight of a seed had highly negative correlation with total precipitation from December of the flowering year to February of the cone production year. The average weight of cone coat was negatively correlated with two climatic variables derived from clear days, which are sum of clear days from November of the flowering year to March of the cone production year and sum of clear days from December of the flowering year to February of the cone production year. On the other hand, it showed positive correlation with mean temperature of May in the flowering year. The exactly same results were obtained in correlation analysis for the percentage of cone coat.

복원된 국지기후에 근거한 잣나무 성숙임분의 영양생장에 미치는 국지기후의 영향

김일현(Il Hyun Kim),신만용(Man Yong Shin),김영채(Young Chai Kim),전상근(Sang Keun Chon) 한국농림기상학회 2001 한국농림기상학회지 Vol.3 No.2

This study was conducted to reveal the effects of local climatic conditions on the vegetative growth in a mature stand of Korean white pine based on climatic estimates. For this, the annual increments of stand variables such as DBH, height, basal area and volume were measured and estimated for seven years from 1974 to 1980. The local climatic conditions in the study site were also estimated by both a topoclimatological method and a spatial statistical technique. The local climatic conditions were then correlated with and regressed on the growth factors to reveal the relationships between the climatic estimates and the growth. It is found that relatively high temperatures had positive effects on the diameter growth. The yearly diameter growth increased when each of mean, maximum, and minimum temperature during the growing season was high. Height growth showed positively significant correlation with three climatic variables. The most important variable influencing height growth was the average of maximum temperature for 10 months from January to October. It means that the higher the average of maximum temperature for 10 months from January to October is, the more height growth of Korean white pine increases. Other climatic variables related to height growth were average of minimum temperature for 3 months in the early growing season and mean relative humidity for the growing season. Six climatic variables related to temperature had effects on basal area increment and all of them were positively correlated with basal area increment. Especially, temperatures from January to March were important factors affecting the basal area increment. In volume increment, high correlation was also recognized with most of temperature variables. This tendency was the same as the results in diameter and hight increments. This means that the volume growth increases when temperatures during the growing season are relatively high.