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폐 섬유모세포에서 황사의 미세먼지(Particulate Matter 10)가 활성산소족과 TGF-β, NF-κB, PDGF-α, Fibronectin의 생성에 미치는 영향
김아현 ( Ah Hyun Kim ),전수연 ( Su Yeon Chon ),윤진영 ( Jin Young Yoon ),김유진 ( Yu Jin Kim ),경선영 ( Sun Young Kyung ),이상표 ( Sang Pyo Lee ),박정웅 ( Jeong Woong Park ),정성환 ( Sung Hwan Jeong ) 대한결핵 및 호흡기학회 2009 Tuberculosis and Respiratory Diseases Vol.67 No.6
Background: Dust clouds blown by the wind from the arid deserts of Mongolia and Northeast China are known as Asian dust storms. Ambient particulate matter with a diameter <10 μm (PM10) is associated with the exacerbation of respiratory diseases and increased mortality of heart and lung disease patients. The fibrotic effects of PM10 of Asian dust to pulmonary fibroblast cells are unknown. This study examined the production of reactive oxygen species (ROS), TGF-β, NF-κB, PDGF-α and Fibronectin in fibroblasts exposed to Asian dust particles. Methods: Air samples were collected using a high volume air sampler (Sibata model HV500F) with an air flow of 500 L/min for at least 6 hours. The MRC-5 cells were exposed to 0, 50 and 100 μg/mL of PM10 for 24 hours. ROS was detected by measuring the level of oxidized DCF using FACS. TGF-β, NF-κB, PDGF-α and fibronectin were detected by western blotting. Results: There was no increase in the ROS, TGF-β and PDGF-α levels in the MRC-5 cells exposed to PM10. The NF-κB level was higher in the MRC-5 cells exposed to 50 and 100 μg/mL of PM10 for 24 hours. The fibronectin level in the MRC-5 cells after 24 hours incubation with 50 μg/mL PM10 was significantly higher than the control group (PM10 50 μg/mL 113.27±8.65 of control, p=0.005). Conclusion: PM10 from Asian dust increases the activation of NF-κB and fibronectin expression in MRC-5 fibroblast cells.
매생이(Capsosiphon fulvescens)를 이용한 김부각의 제조 및 품질특성 연구
김아현 ( Ah-hyun Kim ),홍도희 ( Do-hee Hong ),류아라 ( A-ra Ryu ),조종락 ( Jong-lak Cho ),김정목 ( Jeong-mok Kim ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.2
We evaluated the quality characteristics of Kimbugak prepared using a microwave oven or the oil-frying puffing method with different contents (0%, 15% and 30%) of Capsosiphon fulvescens in dried laver. Microwave-processed Kimbugak had lower crude fat content but higher levels of crude ash, crude protein, and minerals than that prepared by oil-frying; it also had higher hardness values. The redness of Kimbugak containing 30% C. fulvescens processed using either method was lower than the control group. In sensory evaluations, participants preferred Kimbugak containing 30% C. fulvescens puffed by oil-frying. These results indicate that Kimbugak puffed using the oil-frying method has the best quality characteristics.
김경진 ( Kyeong Jin Kim ),김아현 ( Ah Hyun Gim ),김지현 ( Ji Hyun Kim ),김도희 ( Do Hee Kim ),이서린 ( Seo Rin Lee ),박지현 ( Jee Hyun Park ),임지원 ( Ji Won Lim ),하지훈 ( Ji Hoon Ha ),박수남 ( Soo Nam Park ) 대한화장품학회 2015 대한화장품학회지 Vol.41 No.2
본 연구에서는 명월초 추출물의 메탄올 분획물과 아글리콘 분획물을 제조하였고, 이들에 대한 항산화 활성을 측정하였다. 항산화 활성 측정은 1,1-diphenyl-2-picrylhydrazil (DPPH) radical을 이용한 프리 라디칼 소거활성, 루미놀 발광법을 이용한 활성산소 소거활성(총 항산화능) 및 활성산소(<sup>1</sup>O<sub>2</sub> 등)로 유도된 세포손상에 있어서 세포 보호 효과를 측정하였다. 명월초 추출물의 프리 라디칼 소거활성(FSC<sub>50</sub>)은 메탄올 분획이 90.25 μg/mL이고, 당을 제거한 아글리콘 분획은 81.38 μg/mL를 나타내었다. 총 항산화능(OSC<sub>50</sub>)은 메탄올분획 16.96 μg/mL, 아글리콘 분획 12.30 μg/mL로, 프리 라디칼 소거활성 및 활성산소 소거활성 모두 아글리콘 분획에서 큰 활성을 나타내었다. <sup>1</sup>O<sub>2</sub>로 유도된 사람 적혈구의 세포손상에 대한 보호 효과는 τ<sub>50</sub> 값으로 확인하였다. 5 μg/mL 농도에서 메탄올 분획 및 아글리콘 분획의 τ<sub>50</sub>이 각각 36.7 및 76.1 min으로, 비교 물질인(+)-α-tocopherol (35.4 min)과 비교했을 때 아글리콘 분획이 약 2배 더 큰 세포 보호 효과를 나타내었다. 이러한 결과들은 명월초 추출물의 아글리콘 분획물이 항노화 관련 화장품에 있어서 항산화소재로서 응용가능성이 있음을 시사하였다. In this study, the methanol fraction and aglycone fraction were made from Gynura procumbens (G. procumbens) extracts and their antioxidative effects were investigated. The free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH), total antioxidant capacity by luminol-dependent chemiluminescence assay, and the protective effects against reactive oxygen species (ROS) in erythrocytes were measured to evaluate the antioxidative activities of the extracts. Free radical scavenging activities (FSC<sub>50</sub>) of the methanol fraction and aglycone fraction were 90.25 and 81.38 μg/mL, respectively. Total antioxidant capacities (OSC<sub>50</sub>) of the methanol fraction and aglycone fraction were 16.96 and 12.30 μg/mL, respectively. The free radical scavenging activity and total antioxidant capacity of the aglycone fraction were greater than those of methanol fraction. The cellular protective effect on the <sup>1</sup>O<sub>2</sub>-induced cellular damage of human erythrocytes was confirmed by τ<sub>50</sub> value. The τ<sub>50</sub> value of the methanol fraction and agly cone fraction were 36.7 min and 76.1 min, respectively in 5 μg/mL, and the aglycone fraction showed about 2 times higher cellular protecive effect than (+)-α-tocopherol (35.4 min). These results indicate that the aglycone fraction of G. procumbens extracts has application possibility as antioxidant ingredient of cosmetic.
김기화(Ki Hwa Kim),김아현(Ah Hyun Kim),이재근(Jae Keun Lee),전명숙(Myoung Sook Chun),노봉수(Bong Soo Noh) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.1
가공방식을 달리한 커피 원두와 분말을 대상으로 향기패턴 분석한 결과 커피 분말의 경우 washed coffee, pulped natural coffee, natural coffee 순으로 향이 높은 것으로 나타났다. 다음으로는 결점원두를 대상으로 통계 처리한 결과 일반원두에 비해서 향함량이 낮은 것으로 나타났다. 이러한 결과는 향후 커피 원두의 품질관리 및 평가에 적용될 수 있음을 확인하였고 향후 전자코가 커피원두의 등급을 매기는 커피원료전문가(Q-grader)로서의 역할이 기대된다. An ‘electronic nose’ based on mass spectrometer and discriminant function analysis (DFA) was used to evaluate the grade of coffee beans. The data obtained from the electronic nose was analyzed by DFA. The discriminant function first score (DF1) of natural coffee beans showed a greater decrease than the different processing methods. Defective coffee beans were separated well from non-defective coffee beans by DF1, which correlated with a weaker flavor than that of the others. Flavor patterns of the defective and the non-defective coffee beans were determined as complementary information. The flavor patterns obtained in this study can explain, in a simplified way, the differences between the defective and the non-defective coffee beans.