Antihepatotoxic Components of Korean Ginseng: Effect on Lipid Peroxidation

김혜영,이유희,김신일,KimJun, Hye-Young,Lee, You-Hui,Kim, Shin-Il Korean Society for Biochemistry and Molecular Biol 1989 한국생화학회지 Vol.22 No.1

고려인삼에서 분리한 폴리아세틸렌 성분인 파낙시돌, 파낙시놀과 파낙시트리올의 사염화탄소로 유도된 흰쥐의 지질과산화와 효소적 또는 비효소적으로 유도된 시험관내 과산화지질 형성에 마치는 영향을 관찰하였다. 사염화탄소 처리된 흰쥐에 대한 폴리아세틸렌의 효과를 혈청과 간 과산화지칠 수준과 혈청효소(GOT, GPT, LDH) 활성을 측정함으로써 관찰하였다. 간 마이크로좀내 cytochrome P-450 함량과 aniline hydroxylase와 aminopyrine demethylase 활성도 측정되었다. 시험관내 과산화지질 형성을 위해 paraquat/NADPH 또는 ascrobate/$Fe^{+2}$로 과산화지질을 유도하였다. 그 결과로서, 폴리아세틸렌 성분들은 사염화탄소로 유도된 간의 과산화지질 형성에 대한 보호작용올 나타내었고, 파낙시놀은 혈청 지질과산화 수준을 낮추었다. 또한 폴리아세틸렌 성분들은 사염화탄소로 유도되는 LDH의 혈액내 유출에 대한 보호작용이 있으나, 혈청 GOT와 GPT 수준엔 영향을 주지 않았다. 사염화탄소는 cytochrome P-450 함량과 aniline hydroxylase, aminopyrine demethylase 활성을 낮추었으며, 사염화탄소 없이 폴리아세틸렌만 처리한 경우, 파악시돌과 파낙시놀은 aniline hydroxylase를, 세폴리아세틸렌 성분은 aminopyrine demethylase를 유도하였으며, cytochrome P-450에는 영향을 주지 않았다. 실험관내 간 마이크로좀의 지질과산화는 폴리아세틸렌 첨가시 농도에 비례하여 억제되었다. 이러한 결과들은 폴리아세틸렌 성분들이 in vivo와 in vitro 과산화지질 형성을 억제함으로써 사염화탄소로 유도된 간독성에 대한 보호작용이 있음을 시사해주고 있다. Three polyacetylene compounds, panaxydol, panaxynol and panaxytriol were isolated from Korean ginseng and their inhibitory effects on lipid peroxidation in vivo and in vitro were investigated. Lipid peroxide levels both in serum and liver microsome and serum enzyme (GOT, GPT, LDH) activities of $CCl_4$-administered rats were determined after pretreatment of polyacetylenes. Hepatic microsomal cytochrome P-450-dependent monooxygenases were also measured to investigate the mechanism of action. Enzymatic (paraquat/NADPH) and nonenzymatic (ascorbate/$Fe^{+2}$)systems were used for in vitro lipid peroxidation. As results, polyacetylene compounds inhibited liver lipid peroxidation and panaxynol lowered serum lipid peroxide levels caused by $CCl_4$. Polyacetylene pretreatment prevented leakage of LDH to serum but elevated GOT and GPT levels were not changed. $CCl_4$ caused marked losses in cytochrome P-450 and reduced the activities of aniline hydroxylase and aminopyrine demethylase. Polyacetylene treatment without $CCl_4$ induced aminopyrine demethylase and panaxydol and panaxynol induced aniline hydroxylase while the content of cytochrome P-450 were not affected. In vitro microsomal lipid peroxidation was inhibited by polyacetylenes and DL-${\alpha}$-tocopherol in a dose-dependent manner. The results demonstrate that polyacetylenes protected against hepatotoxicity of $CCl_4$ by inhibiting lipid peroxidation in vivo and in vitro possibly through the mechanism of direct action on liver microsomes.

Protective Effect of Panaxytriol Against Tumorigenesis in Mouse Skin

김혜영,이유희,김신일,KimJun, Hye-Young,Lee, You-Hui,Kim, Shin-Il 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

파낙시트리올의 항산화작용을 이용하여 생쥐 표피조직내 glutathione peroxidase 활성, ornithine decarboxylase 유도 및 MCA로 유발된 피부암 발생에 대한 영향을 조사하였다. 파낙시트리올의 파두유로 유도된 생쥐 귀 부종 현상에 대한 효과도 관찰되었다. 결과로서, 파낙시트리올은 파두유로 감소된 glutathione peroxidase와 파두유로 유도된 ornithine decarboxylase에 보호작용을 나타내었으며, 생쥐 귀 부종을 유의적으로 억제하였다. MCA를 도포한 BALB/c 생쥐의 피부암 발생은 파낙시트리올 도포로 피부암의 진전이 감소되었다. 이와 같은 결과들은 파낙시트리올이 발암과정 중 자유라디칼로 야기되는 여러 반응들을 억제함으로써 피부암 발생을 감소시킬 수 있음을 시사해 준다. Based on the antioxidant activity of panaxytriol, its effect on glutathione peroxidase activity, ornithine decarboxylase induction in mouse epidermis and 3-methylcholanthrene (MCA)induced skin tumorigenesis were investigated. The effect of panaxytriol on croton oil-induced edema of mouse ears was also investigated. As results, panaxytriol significantly prevented decrease in gluthathione peroxidase activity and ornithine decarboxylase induction caused by croton oil. In addition, panaxytriol significantly inhibited croton oil-induced edema. In BALB/c mice receiving MCA, panaxytriol afforded protection by delaying the subsequent development of skin tumors. It is suggested that panaxytriol have potential for modifying the risk of skin tumorigenecity, which may be supported in part by protection from free radical-induced reactions.

고려인삼의 간독성에 대한 보호성분 : 과산화지질 형성에 미치는 영향

김헤영,이유희,김신일 ( Hye Young Kim,You Hui Lee,Shin Il Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1

Three polyacetylene compounds, panaxydol, panaxynol and panaxytriol were isolated from Korean ginseng and their inhibitory effects on lipid peroxidation in vivo and in vitro were investigated. Lipid peroxide levels both in serum and liver microsome and serum enzyme (GOT, GPT, LDH) activities of CCl₄ -administered rats were determined after pretreatment of polyacetylenes. Hepatic microsomal cytochrome P-450-dependent monooxygenases were also measured to investigate the mechanism of action. Enzymatic (paraquat/NADPH) and nonenzymatic (ascorbate/Fe^(+2) systems were used for in vitro lipid peroxidation. As results, polyacetylene compounds inhibited liver lipid peroxidation and panaxynol lowered serum lipid peroxide levels caused by CC1₄. Polyacetylene pretreatment prevented leakage of LDH to serum but elevated GOT and GPT levels were not changed. CCl₄ caused marked losses in cytochrome P-450 and reduced the activities of aniline hydroxylase and aminopyrine demethylase. Polyacetylene treatment without CCl₄ induced aminopyrine demethylase and panaxydol and panaxynol induced aniline hydroxylase while the content of cytochrome P-450 were not affected. In vitro microsomal lipid peroxidation was inhibited by polyacetylenes and DL-α-tocopherol in a dose-dependent manner. The results demonstrate that polyacetylenes protected against hepatotoxicity of CCl₄ by inhibiting lipid peroxidation in vivo and in vitro possibly through the mechanism of direct action on liver microsomes.

파낙시트리올의 생쥐 피부암 발생에 대한 보호작용

김혜영,이유희,김신일 ( Hye Young Kim ( Jun ),You Hui Lee,Shin Il Kim ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

Based on the antioxidant activity of panaxytriol, its effect on glutathione peroxidase activity, ornithine decarboxylase induction in mouse epidermis and 3-methylcholanthrene (MCA)induced skin tumorigenesis were investigated. The effect of panaxytriol on croton oil-induced edema of mouse ears was also investigated. As results, panaxytriol significantly prevented decrease in gluthathione peroxidase activity and ornithine decarboxylase induction caused by croton oil. In addition, panaxytriol significantly inhibited croton oil-induced edema. In BALB/c mice receiving MCA, panaxytriol afforded protection by delaying the subsequent development of skin tumors. It is suggested that panaxytriol have potential for modifying the risk of skin tumorigenecity, which may be supported in part by protection from free radical-induced reactions.

보문 : 누룩곰팡이 분리균의 다양성 및 당화능 분석과 독소생산능 조사

김민식 ( Min Sik Kim ),김신일 ( Sin Il Kim ),하병석 ( Byeong Seok Ha ),박혜영 ( Hye Young Park ),백성열 ( Seong Yeol Baek ),여수환 ( Soo Hwan Yeo ),노현수 ( Hyeon Su Ro ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.3

"Nuruk samples collected from various regions in Korea were investigated in terms of fungal contents and diversity. In measurement of colony forming unit (CFU) in Nuruk suspensions on DRBC agar, Nuruk samples MS4, MS8, and MS10 were among the highest fungal density, with 1,278.9±21.6 (×104), 1,868.0±27.7 (×104), and 775.1±19.2 (×104) were among the samples showing the highest fungal density. CFU per 20 mg Nuruk, respectively. The majority of fungal components were yeasts, including Pichia anomala, P. kudriavzevii, Kluyveromyces marxianus, and Saccharomycopsis fibuligera, whereas Aspergillus oryzae and Rhizopus oryzae, the representative Nuruk fungi, were predominant only in the low fungal density Nuruks (MS2, MS5, and MS11). Saccharification capability of the fungal isolates was assessed by measurement of amylase activity in the culture broth. The highest amylase activity was found in A. niger and A. luchuensis, followed by S. fibuligera. A. oryzae and R. oryzae showed fair amylase activity but significantly lower than those of the three fungal species. R. oryzae was suggested to play an additional role in degradation of β-glucan in crop component of Nuruk since R. oryzae was the only fungus that showed β-glucanase activity among the fungal isolates. To confirm the safety of Nuruk, aflatoxigenicity of the isolated Aspergillus was estimated using the DNA markers norB-cypA, aflR, and omtA. All of the isolates turned out to be non-aflatoxigenic as evidenced by the deletion of gene markers, norB-cypA and aflR, and the absence of aflatoxin in the culture supernatants shown by TLC analysis."