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Baeuveria sp. C208의 대량 배양을 위한 생산배지의 최적화
문기혁,김병혁,윤정원,성재모,김승욱,Moon, Ki-Hyuk,Kim, Pyong-Hyok,Yoon, Jeong-Weon,Sung, Jae-Mo,Kim, Seung-Wook 한국미생물 · 생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.6
Entomogenous fungi which attack living insects are powerful means for microbiological insecticide. The purpose of this study is to establish the culture conditions and media for mass production of Beauveria sp. C208 which has a broad host range as a potential microbiological pesticide. The temperature and pH range for optimal cultivation of this strain were 28$circ$C and pH 5.0-7.0. For Beauveria sp. C 208, 2% rice straw and 0.6% tryptone were found as the proper carbon and nitrogen sources, considering cell mass, enzyme activities such as chitinase, protease and lipase, and spore concentration.
Metarhizium anisoplies ARS 2231 의 대량배양을 위한 생산배지의 최적화
김승욱,강성우,윤정원,문기혁 고려대학교 공학기술연구소 1999 공학논문집 Vol.37 No.1
Chemical pesticides which have been commonly used for crop protection have many problems such as high production cost and environmental hazards. Entomogeneous fungi which attack living insects are very powerful means for microbiological insecticide. The purpose of this study is to investigate the culture conditions for mass production of Metarhizium anisopliae ARS 2231 which is a potential microbiological pesticide. The temperature and pH range for optimal cultivation were 28℃ and pH 5.0∼7.0, respectively. For M. anisopliae ARS 2231, 3.0% (w/v) rice bran, 0.8% (w/v) hydrolyzed casein, and 0.3% (w/v) K₂HPO₄ were found as the proper nutrients, considering cell mass, enzyme activities, and spore concentration.