TOD기반 복합환승센타/역세권 개발 및 활성화 방안

김문섭,이부원,최기주,Kim, Moon-Sup,Lee, Boo-Won,Choi, Kee-Choo 대한교통학회 2011 교통 기술과 정책 Vol.8 No.1

휠-레일 점착 퍼지 모델에 의한 고속전철의 주행 시뮬레이션에 관한 연구

김문섭(Moon Sup Kim),김동우(Dong Woo Kim),신두진(Doo Jin Shin),허욱열(Uk Youl Huh) 대한전기학회 1998 대한전기학회 학술대회 논문집 Vol.1998 No.11

광나무 꽃의 꿀벌 방화와 화밀 분비 특성

김문섭(Kim Moon Sup),김세현(Kim Sea Hyun),송정호(Song Jeong Ho),김혜수(Kim Hyeusoo),오득실(Oh Deuk Sil) 한국양봉학회 2014 韓國養蜂學會誌 Vol.29 No.4

This study was conducted to provide a basic data such as honeybee visiting and secreted nectar for possibility of development as a honey plant, Ligustrum japonicum Thunb. in Jeonnam Wando Arboretum. The surveyed trees flowers bloom and secrete nectar during 7 days in 2014. It is showed that more Apis mellifera visited flowers in priority than other pollinators, so the percentage of A. mellifera which visited flowers was 57%. In addition, number of visited honeybees per flowering lateral bunch can be assumed 173.8 honeybees for a day. Between visited number of A. mellifera and meteorologic traits (temperature, relative humidity) signified correlation, which mean that honeybee activity was influenced by temperature and relative humidity. Secondly, we found that both secreted nectar volume is more secreted in the morning. On average, volume of total and dry nectar secreted by nectary were 1.07 ul and 0.15 ul from one flower, respectively. Finally, the surveyed L. japonicum is considered as possible sub-honey plant because of its nectar volume and visited honeybee data.

황칠나무 꽃의 곤충 방화, 화밀의 분비 특성과 당 함량 및 아미노산 분석

김문섭(Kim Moon-Sup),김혜수(Kim Hyeusoo),김소담(Kim So-Dam),박상진(Park Sang-Jin),송정호(Song Jeong-Ho),김세현(Kim Sea-Hyun) 한국양봉학회 2015 韓國養蜂學會誌 Vol.30 No.4

This study was conducted to provide a basic data such as honeybee visit and secreted nectar for possibility of development as a honey plant, Dendropanax morbifera Lev. The surveyed tree’s flowers bloom during 23 days in U-san park, Gwang-yang city in 2014. It is showed that more Apis mellifera visited flowers in priority than other pollinators, so the percentage of A. mellifera which visited flowers was 69%. And number of visited honeybees per flowering lateral bunch can be assumed 47.3 honeybees for a day. Nectar volume secreted by nectary was 4.5㎕ and dry nectar volume was 0.48㎕ from one flower. Nectar concentration showed 20.9% and sugar contents of a flower is 79.4㎍, averagely. So, the minimum estimates of honey harvest for a flower was 93.4㎍. Analysis of amino acid showed that Phenylalanine, Glutamine and Proline were more abundant. Especially, Phenylalanine ratio shows 67.2%. Finally, the surveyed D. morbifera is considered as possible sub-honey plant because of its visited honeybee data and nectar characteristics such as nectar sugar and amino acid contents.

밀원수종 아까시나무의 근삽시 발근촉진제와 삽목시기 효과

김문섭(Kim Moon Sup),김혜수(Kim Hyeusoo),김세현(Kim Sea Hyun) 한국양봉학회 2015 韓國養蜂學會誌 Vol.30 No.4

As a part of the breeding program to preserve and cultivate of Robinia pseudoacacia L. which is the major honey tree of Korea, this study was conducted to demonstrate effects of growth regulators, cutting time and shoot rate on cutting position and growth patterns. In terms of effects of growth regulators, IBA 1,000ppm which is the dipping treated group showed the highest rooting rate, 87%. The soaking treated group such as IBA 100ppm and IBA 500ppm, which had the rooting rate of 85% and IBA 50ppm had the rooting rate of 78%. Also, IBA 2,000ppm had the rooting rate of 65%, by the way the control groups show rooting rate of 45%. This proved effects of growth regulators on the rooting rate. There was, also, a significant difference between treatments with Duncan’s multiple range test. Even though the beginning of sprouting delayed to 3~4 weeks after cutting, the earlier cutting time was, the higher the shoot rate after 10 weeks got. The shoot rate of a group cut on March 25 and April 5 were over 85%. On the other hand, the later cutting time was, the more even the shoot developed in each period. In respect of the growth of sapling, the group cut on April 5 were all good in seedling height, ramification, diameter of shoots. As a rooting rate by cuttings position of root cuttings, the middle part of root showed the highest rooting rate, 88%. The upper part’s show 83% and the lower part’s show rooting rate of 62%

중국 산사나무의 꿀벌 방화와 화밀 분비 특성

김문섭(Moon Sup Kim),김세현(Sea Hyun Kim),한진규(Jingyu Han),강문수(Moon Su Kang),박영기(Young Ki Park) 한국양봉학회 2011 韓國養蜂學會誌 Vol.26 No.2

This study was conducted to provide a basic data such as honeybee visiting and nectar secretion for possibility of development as a new honey plant, Crataegus pinnatifida Bunge in Suwon. The whole flowers of individual trees bloom during the 12days in 2010. Each of the flowers absorbs and secretes nectar that lasted for 3days in the some flowers. The honeybee visiting increases when cumulative flowering rate increases, however, gradually honeybee visiting decreases when the whole flowers of individual trees are in full bloom. The amount of nectar secretion is 13.80±2.7μl, which value is average, from one flower. Nectar secretion pattern per one flower dunring the 2days is diverse. After first dehiscence, the amount of nectar secretion at the next 9 A.M. is bigger quantity than the others value. The surveyed C. pinnatifida is considered as possible major honey tree because its amount of nectar secretion is higher than the some species such as Robinia pseudoacacia (2.20±1.2㎕), Tilia amurensis (3.58±1.0㎕), Hovenia dulcis (4.15±1.1㎕) that extracted precedent study.

2분과 : 밀원수종 쉬나무 수꽃과 암꽃의 화밀분비량, 당 함량 및 아미노산 분석

김문섭 ( Moon Sup Kim ),김세현 ( Sea Hyun Kim ),송정호 ( Jeong Ho Song ),김혜수 ( Hyeu Soo Kim ) 한국임학회 2014 한국산림과학회지 Vol.103 No.1

본 연구는 밀원수종으로 관심의 대상이 되고 있는 쉬나무를 대상으로 수꽃과 암꽃의 화밀 분비량, 당 함량및 아미노산을 분석하고 수확할 수 있는 꿀의 양을 추정하기 위하여 실시하였다. 경기도 화성시의 2012년 쉬나무 수꽃의 개화 최성기는 7월 24일부터 26일이었으며, 평균 화밀분비량은 2.73±0.73 μL, 화밀 당도는 17.4%를 나타냈다. 2012년 암꽃의 개화 최성기는 8월 7일부터 9일이었으며 평균 화밀분비량은 0.63±0.49 μL, 화밀 당도는 25.7%를 나타냈다. 기상인자와 상관분석 결과, 화밀 분비량과 화밀 당도는 온도와 습도의 영향을 많이 받는다는 것을 알 수 있었다. 반면, 꽃 한 개당 당 함량을 산출한 결과, 수꽃은 48.0±5.2 μg, 암꽃은 37.8±8.7 μg으로 분석되었으나, 유의적인 차이는 없었다(Mann-Whitney’s U-test, p=0.400). 이러한 당 함량을 가지고 수꽃 1개 화서에서는 최소 67.8 g, 암꽃 1개 화서에서는 최소 53.3 g의 꿀을 수확할 수 있을 것으로 추정하였다. 또한 아미노산 분석 결과, Serine, Glycine및 Alanine은 수꽃에서 더 많은 함량이 검출된 반면, Asparatate, Glutamate, Asparagine 및 Glutamine은 암꽃에서 더많은 함량을 나타내어 수꽃과 암꽃에서 7개의 아미노산 함량이 유의적으로 차이가 있음을 알 수 있었다. The purpose of study was to analyze secreted nectar volume, nectar sugar content and amino acid in addition to estimating honey quantities that can ultimately reap in male and female flowers of Evodia danielliiHemsl.. The maximum blooming period of male flowers was on 24 to 26 July in 2012. On average, nectarvolume secreted by nectary was 2.73±0.73 μL from one male flower and nectar concentration showed 17.4%. The maximum blooming period of female flowers was on 7 to 9 August in 2012. Nectar volume secreted bynectary was 0.63±0.49 μL from one female flower and nectar concentration showed 25.7%, averagely. As results of correlation analysis between the meteorological factors and nectar characteristics, we found that nectar quantities and concentration were influenced by temperature and relative humidity. Sugar content was calculated at 48.0±5.2 μg per a male flower and 37.8±8.7 μg per a female flower, which meant that both values were not significantly different (Mann-Whitney`s U-test, p=0.400). The minimum estimates of honey harvest for a male and female inflorescence were 67.8 g and 53.5 g, respectively. Analysis of amino acid showed that Serine, Glycine and Alanine were more abundant in male flowers, however Asparatate, Glutamate, Asparagine and Glutamine were more abundant in female flowers.

산수유나무 선발집단의 꽃의 형태적 특성과 선발효과 추정

김문섭(Moon Sup Kim),한진규(Jingyu Han),김혜수(Hyeusoo Kim),김세현(Sea Hyun Kim) 한국양봉학회 2012 韓國養蜂學會誌 Vol.27 No.4

A member of the Cornaceae family, Cornus officinalis S. et Z. is a China native tree, which is deciduous and sub tall tree. It can grow to a height of 7m and up to 40m wide. Ecologically, this shrub grows well in hillside thickets. This research investigates quantitative morphological characteristics of flower among the C. officinalis in South Korea and then considers its relationship on the basis of raw data among the 10 selected populations. This study will give us invaluable information about growing condition, reasonable management and breeding by selection of C. officinalis in South Korea. The main results obtained from this study are summarized as follows; Suwon and Gunwi populations were larger in flower characteristics compared with other populations and Namwon population was smallest among populations. According to cluster analysis based on the flower morphological characteristics, the natural C. officinalis populations were classified into two groups such as the first group of Gurye, Suncheon, Jinju, Namwon, Hwasun population, the second group of Gunwi, Suwon, Chuncheon, Chungju, Icheon. Selection effects with 5% selection level was estimated at 130.3% in number of inflorescence per fructify lateral branch, 125.2% in number of flower per inflorescence and 121.9% in width of calyx.

섬피나무의 꿀벌 방화 및 화밀 분비 특성과 기상과의 관계

김문섭(Kim Moon Sup),김세현(Kim Sea Hyun),송정호(Song Jeong Ho),김혜수(Kim Hyeusoo) 한국양봉학회 2013 韓國養蜂學會誌 Vol.28 No.5

This study was conducted to provide a basic data such as honeybee visiting and secreted nectar for possibility of development as honey plant, Tilia insularis Nakai in Suwon. The surveyed trees flowers bloom and secrete nectar during 7 days in 2012. One of the flowers secretes and absorbs nectar, which lasted for 2 days. Apis mellifera and Bombus ignitus visited flowers in priority, number of visited pollinator per flowering lateral bunch can be assumed 160 honeybees and 46 carpenter bees for a day. Honeybee visiting was concentrated at around 3 pm. On average, volume of total and dry nectar secreted by nectary were 12.0±4.5μl and 1.0±0.4μl from one flower, respectively. As results of correlation analysis between the meteorological traits and surveyed data, we found that honeybees activity was influenced by air temperature and relative humidity. Volume of total nectar was influenced by mostly meteorological traits and volume of dry nectar was less influenced by air temperature and relative humidity, respectively. To select honey plant or individual tree, volume of dry nectar must be major judgement factor on breeding because it was less influenced by meteorological traits than volume of total nectar. Finally, the surveyed T. insularis is considered as possible honey plant because of its nectar volume and honeybee visiting. Honey is made of nectar sugar as well as by digestive system of honeybee, therefore a more associated study is necessary to analyze free sugar component in nectar and accurately presume to harvest honey quantity.

한국에서 수집된 돌배나무의 유전적 다양성

김문섭(Moon-Sup Kim),김세현(Sea-Hyun Kim),한진규(Jin-Gyu Han),송정호(Jeong-Ho Song),김혜수(Hyeu-Soo Kim),김도훈(Do-Hoon Kim),권용삼(Yong-Sham Kwon) 한국육종학회 2015 한국육종학회지 Vol.47 No.1

The objective of this study was carried out to assess genetic diversity of Korean local accessions of wild pear using morphological traits and microsatellite markers. Assessment of 14 phenotypic traits showed high variation among wild pear accessions. These parameters were not applicable genetic diversity analysis of wild pear collection due to quantitatively inherit and their expression is affected by environmental factors. Microsatellite markers were used to evaluate the genetic similarity of 62 accessions. Among 50 tested SSR markers, 16 primer pairs were selected to profile genetic diversity on the basis of high level polymorphism. These microsatellite markers showed 1 or 2 discrete amplified fragment for all of the accessions. The sixteen microsatellite loci amplified 278 alleles, with 10 to 27 alleles (average 17.385) per locus. The mean of observed heterozygosity and polymorphism information content were 0.776 and 0.836, respectively. The Jaccard’s similarity coefficient ranged from 0.09 to 1.00. Two major groups were produced from all the accessions by UPGMA cluster analysis. Fifty five accessions could be discriminated except for 2 pairs. These results would be provided useful information about valuable genetic resources though assessment of genetic diversity and relationship in local pear accessions.